Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 41
Filtrar
1.
Bioinformatics ; 39(7)2023 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-37449883

RESUMEN

SUMMARY: Viral genes, that are frequently small genes and/or with large overlaps, are still difficult to predict accurately. To help predict all genes in viral genomes, we provide CodingDiv that detects SNP-level microdiversity of all potential coding regions, using metagenomic reads and/or similar sequences from external databases. Protein coding regions can then be identified as the ones containing more synonymous SNPs than unfavorable nonsynonymous substitutions SNPs. AVAILABILITY AND IMPLEMENTATION: CodingDiv is released under the GPL license. Source code is available at https://github.com/ericolo/codingDiv. The software can be installed and used through a docker container.


Asunto(s)
Polimorfismo de Nucleótido Simple , Programas Informáticos , Bases de Datos Factuales , Genoma Viral , Metagenómica
2.
Curr Microbiol ; 81(9): 266, 2024 Jul 14.
Artículo en Inglés | MEDLINE | ID: mdl-39003664

RESUMEN

DNA Stable Isotope Probing is emerging as a potent methodology for investigating host-virus interactions, based on the essential reliance of viruses on host organisms for the production of virions. Despite the anticipated link between host isotopic compositions and the generated virions, the application of stable isotope probing to viral DNA has never been evaluated on simple biological models. In this study, we assessed the efficacy of this method on the bacteriophage T4 and its host, Escherichia coli. Through the cultivation of E. coli cells on a 13C-enriched substrate and subsequent propagation of T4 bacteriophage, we examine the degree of isotopic enrichment in viral DNA. Our investigation reveals a strong correlation between the proportion of 13C6-D-glucose in the growth substrate and the buoyant density in CsCl gradient of T4 DNA, confirming the validity of DNA SIP in viral ecology. These findings underscore the potential of DNA SIP as a robust tool for characterizing the diversity of viruses infecting hosts with specific metabolic activities and provide then a foundation for further exploration in viral ecology research.


Asunto(s)
Bacteriófago T4 , ADN Viral , Escherichia coli , Bacteriófago T4/genética , Bacteriófago T4/fisiología , Bacteriófago T4/metabolismo , Escherichia coli/virología , Escherichia coli/genética , Escherichia coli/metabolismo , ADN Viral/genética , Interacciones Microbiota-Huesped , Glucosa/metabolismo
3.
Arch Virol ; 168(2): 74, 2023 Jan 23.
Artículo en Inglés | MEDLINE | ID: mdl-36683075

RESUMEN

This article summarises the activities of the Bacterial Viruses Subcommittee of the International Committee on Taxonomy of Viruses for the period of March 2021-March 2022. We provide an overview of the new taxa proposed in 2021, approved by the Executive Committee, and ratified by vote in 2022. Significant changes to the taxonomy of bacterial viruses were introduced: the paraphyletic morphological families Podoviridae, Siphoviridae, and Myoviridae as well as the order Caudovirales were abolished, and a binomial system of nomenclature for species was established. In addition, one order, 22 families, 30 subfamilies, 321 genera, and 862 species were newly created, promoted, or moved.


Asunto(s)
Bacteriófagos , Caudovirales , Siphoviridae , Virus , Humanos , Virus/genética , Myoviridae
4.
Environ Microbiol ; 24(10): 4853-4868, 2022 10.
Artículo en Inglés | MEDLINE | ID: mdl-35848130

RESUMEN

Diversity of viruses infecting non-extremophilic archaea has been grossly understudied. This is particularly the case for viruses infecting methanogenic archaea, key players in the global carbon biogeochemical cycle. Only a dozen of methanogenic archaeal viruses have been isolated so far. In the present study, we implemented an original coupling between stable isotope probing and complementary shotgun metagenomic analyses to identify viruses of methanogens involved in the bioconversion of formate, which was used as the sole carbon source in batch anaerobic digestion microcosms. Under our experimental conditions, the microcosms were dominated by methanogens belonging to the order Methanobacteriales (Methanobacterium and Methanobrevibacter genera). Metagenomic analyses yielded several previously uncharacterized viral genomes, including a complete genome of a head-tailed virus (class Caudoviricetes, proposed family Speroviridae, Methanobacterium host) and several near-complete genomes of spindle-shaped viruses. The two groups of viruses are predicted to infect methanogens of the Methanobacterium and Methanosarcina genera and represent two new virus families. The metagenomics results are in good agreement with the electron microscopy observations, which revealed the dominance of head-tailed virus-like particles and the presence of spindle-shaped particles. The present study significantly expands the knowledge on the viral diversity of viruses of methanogens.


Asunto(s)
Virus de Archaea , Virus , Archaea/genética , Carbono , Formiatos , Genoma Viral , Isótopos , Metagenómica/métodos , Methanobacterium , Virus/genética
5.
Syst Biol ; 69(1): 110-123, 2020 01 01.
Artículo en Inglés | MEDLINE | ID: mdl-31127947

RESUMEN

Tailed bacteriophages are the most abundant and diverse viruses in the world, with genome sizes ranging from 10 kbp to over 500 kbp. Yet, due to historical reasons, all this diversity is confined to a single virus order-Caudovirales, composed of just four families: Myoviridae, Siphoviridae, Podoviridae, and the newly created Ackermannviridae family. In recent years, this morphology-based classification scheme has started to crumble under the constant flood of phage sequences, revealing that tailed phages are even more genetically diverse than once thought. This prompted us, the Bacterial and Archaeal Viruses Subcommittee of the International Committee on Taxonomy of Viruses (ICTV), to consider overall reorganization of phage taxonomy. In this study, we used a wide range of complementary methods-including comparative genomics, core genome analysis, and marker gene phylogenetics-to show that the group of Bacillus phage SPO1-related viruses previously classified into the Spounavirinae subfamily, is clearly distinct from other members of the family Myoviridae and its diversity deserves the rank of an autonomous family. Thus, we removed this group from the Myoviridae family and created the family Herelleviridae-a new taxon of the same rank. In the process of the taxon evaluation, we explored the feasibility of different demarcation criteria and critically evaluated the usefulness of our methods for phage classification. The convergence of results, drawing a consistent and comprehensive picture of a new family with associated subfamilies, regardless of method, demonstrates that the tools applied here are particularly useful in phage taxonomy. We are convinced that creation of this novel family is a crucial milestone toward much-needed reclassification in the Caudovirales order.


Asunto(s)
Caudovirales/clasificación , Filogenia , Caudovirales/genética , Clasificación , Genoma Viral/genética
6.
Arch Virol ; 166(11): 3239-3244, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-34417873

RESUMEN

In this article, we - the Bacterial Viruses Subcommittee and the Archaeal Viruses Subcommittee of the International Committee on Taxonomy of Viruses (ICTV) - summarise the results of our activities for the period March 2020 - March 2021. We report the division of the former Bacterial and Archaeal Viruses Subcommittee in two separate Subcommittees, welcome new members, a new Subcommittee Chair and Vice Chair, and give an overview of the new taxa that were proposed in 2020, approved by the Executive Committee and ratified by vote in 2021. In particular, a new realm, three orders, 15 families, 31 subfamilies, 734 genera and 1845 species were newly created or redefined (moved/promoted).


Asunto(s)
Virus de Archaea/clasificación , Bacteriófagos/clasificación , Sociedades Científicas/organización & administración , Archaea/virología , Bacterias/virología
7.
Int J Mol Sci ; 22(24)2021 Dec 14.
Artículo en Inglés | MEDLINE | ID: mdl-34948244

RESUMEN

The virome associated with the corkscrew shaped bacterium Leptospira, responsible for Weil's disease, is scarcely known, and genetic tools available for these bacteria remain limited. To reduce these two issues, potential transposable prophages were searched in Leptospiraceae genomes. The 236 predicted transposable prophages were particularly abundant in the most pathogenic leptospiral clade, being potentially involved in the acquisition of virulent traits. According to genomic similarities and phylogenies, these prophages are distantly related to known transposable phages and are organized into six groups, one of them encompassing prophages with unusual TA-TA ends. Interestingly, structural and transposition proteins reconstruct different relationships between groups, suggesting ancestral recombinations. Based on the baseplate phylogeny, two large clades emerge, with specific gene-contents and high sequence divergence reflecting their ancient origin. Despite their high divergence, the size and overall genomic organization of all prophages are very conserved, a testimony to the highly constrained nature of their genomes. Finally, similarities between these prophages and the three known non-transposable phages infecting L. biflexa, suggest gene transfer between different Caudovirales inside their leptospiral host, and the possibility to use some of the transposable prophages in that model strain.


Asunto(s)
Genoma Bacteriano , Genoma Viral , Leptospira , Filogenia , Profagos/genética , Enfermedad de Weil/genética , Humanos , Leptospira/genética , Leptospira/virología , Análisis de Secuencia de ADN
8.
9.
Bioinformatics ; 33(19): 3113-3114, 2017 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-28957499

RESUMEN

SUMMARY: WIsH predicts prokaryotic hosts of phages from their genomic sequences. It achieves 63% mean accuracy when predicting the host genus among 20 genera for 3 kbp-long phage contigs. Over the best current tool, WisH shows much improved accuracy on phage sequences of a few kbp length and runs hundreds of times faster, making it suited for metagenomics studies. AVAILABILITY AND IMPLEMENTATION: OpenMP-parallelized GPL-licensed C ++ code available at https://github.com/soedinglab/wish. CONTACT: clovis.galiez@mpibpc.mpg.de or soeding@mpibpc.mpg.de. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.


Asunto(s)
Bacteriófagos/genética , Metagenómica/métodos , Programas Informáticos , Archaea/virología , Bacterias/virología , Mapeo Contig
10.
Environ Microbiol ; 18(3): 889-903, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26472517

RESUMEN

Microbial communities from hypersaline ponds, dominated by halophilic archaea, are considered specific of such extreme conditions. The associated viral communities have accordingly been shown to display specific features, such as similar morphologies among different sites. However, little is known about the genetic diversity of these halophilic viral communities across the Earth. Here, we studied viral communities in hypersaline ponds sampled on the coast of Senegal (8-36% of salinity) using metagenomics approach, and compared them with hypersaline viromes from Australia and Spain. The specificity of hyperhalophilic viruses could first be demonstrated at a community scale, salinity being a strong discriminating factor between communities. For the major viral group detected in all samples (Caudovirales), only a limited number of halophilic Caudovirales clades were highlighted. These clades gather viruses from different continents and display consistent genetic composition, indicating that they represent related lineages with a worldwide distribution. Non-tailed hyperhalophilic viruses display a greater rate of gene transfer and recombination, with uncharacterized genes conserved across different kind of viruses and plasmids. Thus, hypersaline viral communities around the world appear to form a genetically consistent community that are likely to harbour new genes coding for enzymes specifically adapted to these environments.


Asunto(s)
Caudovirales/genética , Genoma Viral/genética , Estanques/virología , Salinidad , Australia , Caudovirales/aislamiento & purificación , Mapeo Cromosómico , Variación Genética , Metagenómica , Senegal , España
11.
BMC Bioinformatics ; 15: 76, 2014 Mar 19.
Artículo en Inglés | MEDLINE | ID: mdl-24646187

RESUMEN

BACKGROUND: Metagenomics, based on culture-independent sequencing, is a well-fitted approach to provide insights into the composition, structure and dynamics of environmental viral communities. Following recent advances in sequencing technologies, new challenges arise for existing bioinformatic tools dedicated to viral metagenome (i.e. virome) analysis as (i) the number of viromes is rapidly growing and (ii) large genomic fragments can now be obtained by assembling the huge amount of sequence data generated for each metagenome. RESULTS: To face these challenges, a new version of Metavir was developed. First, all Metavir tools have been adapted to support comparative analysis of viromes in order to improve the analysis of multiple datasets. In addition to the sequence comparison previously provided, viromes can now be compared through their k-mer frequencies, their taxonomic compositions, recruitment plots and phylogenetic trees containing sequences from different datasets. Second, a new section has been specifically designed to handle assembled viromes made of thousands of large genomic fragments (i.e. contigs). This section includes an annotation pipeline for uploaded viral contigs (gene prediction, similarity search against reference viral genomes and protein domains) and an extensive comparison between contigs and reference genomes. Contigs and their annotations can be explored on the website through specifically developed dynamic genomic maps and interactive networks. CONCLUSIONS: The new features of Metavir 2 allow users to explore and analyze viromes composed of raw reads or assembled fragments through a set of adapted tools and a user-friendly interface.


Asunto(s)
Genoma Viral , Metagenoma , Metagenómica/métodos , Humanos , Intestinos/virología , Filogenia , Programas Informáticos
13.
Virologie (Montrouge) ; 17(4): 229-242, 2013 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-31910596

RESUMEN

Viruses are the most abundant biological entities observed in environmental samples. They display an extraordinary morphological and genetic richness. In addition to their pathogenicity, viruses are now considered as of major influence on biogeochemical cycles, microorganisms' population regulation and more generally on the evolution of cellular genomes throughout the history of life. Viral metagenomics, i.e. the random sequencing of encapsidated nucleic acids in samples, has provided important new insights into viral diversity. These data reveals an overwhelming genetic diversity in the biosphere's viral communities sampled so far, especially in marine environments. Generally, the type of biome or environmental niche from which samples were obtained seems to be the main determinant of its composition. In addition, virome sequences obtained are generally quite distant from the reference sequences available in databases. This fact leads to the inescapable conclusion that nearly all of the virosphere's vast population is currently unknown to science, and emphasizes the need for pushing efforts toward surveying and characterizing the viral diversity.

14.
Virus Evol ; 9(1): veac123, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36694818

RESUMEN

Small circular single-stranded DNA viruses of the Microviridae family are both prevalent and diverse in all ecosystems. They usually harbor a genome between 4.3 and 6.3 kb, with a microvirus recently isolated from a marine Alphaproteobacteria being the smallest known genome of a DNA phage (4.248 kb). A subfamily, Amoyvirinae, has been proposed to classify this virus and other related small Alphaproteobacteria-infecting phages. Here, we report the discovery, in meta-omics data sets from various aquatic ecosystems, of sixteen complete microvirus genomes significantly smaller (2.991-3.692 kb) than known ones. Phylogenetic analysis reveals that these sixteen genomes represent two related, yet distinct and diverse, novel groups of microviruses-amoyviruses being their closest known relatives. We propose that these small microviruses are members of two tentatively named subfamilies Reekeekeevirinae and Roodoodoovirinae. As known microvirus genomes encode many overlapping and overprinted genes that are not identified by gene prediction software, we developed a new methodology to identify all genes based on protein conservation, amino acid composition, and selection pressure estimations. Surprisingly, only four to five genes could be identified per genome, with the number of overprinted genes lower than that in phiX174. These small genomes thus tend to have both a lower number of genes and a shorter length for each gene, leaving no place for variable gene regions that could harbor overprinted genes. Even more surprisingly, these two Microviridae groups had specific and different gene content, and major differences in their conserved protein sequences, highlighting that these two related groups of small genome microviruses use very different strategies to fulfill their lifecycle with such a small number of genes. The discovery of these genomes and the detailed prediction and annotation of their genome content expand our understanding of ssDNA phages in nature and are further evidence that these viruses have explored a wide range of possibilities during their long evolution.

15.
Nat Microbiol ; 8(5): 986-998, 2023 05.
Artículo en Inglés | MEDLINE | ID: mdl-37037943

RESUMEN

The gut microbiome is shaped through infancy and impacts the maturation of the immune system, thus protecting against chronic disease later in life. Phages, or viruses that infect bacteria, modulate bacterial growth by lysis and lysogeny, with the latter being especially prominent in the infant gut. Viral metagenomes (viromes) are difficult to analyse because they span uncharted viral diversity, lacking marker genes and standardized detection methods. Here we systematically resolved the viral diversity in faecal viromes from 647 1-year-olds belonging to Copenhagen Prospective Studies on Asthma in Childhood 2010, an unselected Danish cohort of healthy mother-child pairs. By assembly and curation we uncovered 10,000 viral species from 248 virus family-level clades (VFCs). Most (232 VFCs) were previously unknown, belonging to the Caudoviricetes viral class. Hosts were determined for 79% of phage using clustered regularly interspaced short palindromic repeat spacers within bacterial metagenomes from the same children. Typical Bacteroides-infecting crAssphages were outnumbered by undescribed phage families infecting Clostridiales and Bifidobacterium. Phage lifestyles were conserved at the viral family level, with 33 virulent and 118 temperate phage families. Virulent phages were more abundant, while temperate ones were more prevalent and diverse. Together, the viral families found in this study expand existing phage taxonomy and provide a resource aiding future infant gut virome research.


Asunto(s)
Bacteriófagos , Microbioma Gastrointestinal , Lactante , Humanos , Estudios Prospectivos , Bacteriófagos/genética , Lisogenia , Heces/microbiología , Microbioma Gastrointestinal/genética , Bacterias/genética
16.
Bioinformatics ; 27(21): 3074-5, 2011 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-21911332

RESUMEN

SUMMARY: Metavir is a web server dedicated to the analysis of viral metagenomes (viromes). In addition to classical approaches for analyzing metagenomes (general sequence characteristics, taxonomic composition), new tools developed specifically for viral sequence analysis make it possible to: (i) explore viral diversity through automatically constructed phylogenies for selected marker genes, (ii) estimate gene richness through rarefaction curves and (iii) perform cross-comparison against other viromes using sequence similarities. Metavir is thus unique as a platform that allows a comprehensive virome analysis. AVAILABILITY: Metavir is freely available online at: http://metavir-meb.univ-bpclermont.fr. CONTACT: simon.roux@univ-bpclermont.fr. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.


Asunto(s)
Genoma Viral , Metagenómica/métodos , Filogenia , Programas Informáticos , Virus/clasificación , Internet
18.
Med Sci (Paris) ; 38(12): 999-1007, 2022 Dec.
Artículo en Francés | MEDLINE | ID: mdl-36692279

RESUMEN

Despite their large number, viruses present in the environment remain largely unknown. Metagenomic approaches, targeting viruses specifically or not, have allowed us a better understanding of the composition of natural viral communities, with Caudoviricetes, Microviridae, Cressdnaviricota or Phycodnaviridae being the most frequently found viral groups. Metagenomes are gradually revealing the extent of the diversity of these groups and their structure, highlighting the large number of species, genera and even viral families, most of which being seen for the first time. Within these groups, the gene content, infected hosts and inhabited ecosystems are often consistent with the evolutionary history traced with marker genes. Thus, the diversity of viruses and their genes is more a reflection of their ancient origin and long coevolution with their hosts than of their ability to mutate rapidly.


Title: Mieux connaître les virus présents sur Terre grâce aux métagénomes. Abstract: En dépit de leur très grand nombre, les virus qui peuplent l'environnement restent largement méconnus. Les approches de métagénomique ont permis depuis vingt ans de mieux connaître la composition des communautés virales naturelles, notamment les groupes viraux les plus fréquemment trouvés, et de lever peu à peu le voile sur l'étendue de leur diversité, révélant le grand nombre d'espèces, de genres et même de familles virales, pour la plupart identifiés pour la première fois. Au sein de ces groupes, le contenu en gènes, les hôtes infectés et les écosystèmes habités sont souvent cohérents avec l'histoire évolutive, reflet de l'origine très ancienne des virus et de leur très longue coévolution avec leurs hôtes, plus que de leur capacité à muter rapidement.


Asunto(s)
Metagenoma , Virus , Humanos , Ecosistema , Filogenia , Virus/genética , Evolución Biológica , Genoma Viral
19.
Virus Evol ; 8(2): veac070, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36533142

RESUMEN

The Microviridae family represents one of the major clades of single-stranded DNA (ssDNA) phages. Their cultivated members are lytic and infect Proteobacteria, Bacteroidetes, and Chlamydiae. Prophages have been predicted in the genomes from Bacteroidales, Hyphomicrobiales, and Enterobacteriaceae and cluster within the 'Alpavirinae', 'Amoyvirinae', and Gokushovirinae. We have isolated 'Ascunsovirus oldenburgi' ICBM5, a novel phage distantly related to known Microviridae. It infects Sulfitobacter dubius SH24-1b and uses both a lytic and a carrier-state life strategy. Using ICBM5 proteins as a query, we uncovered in publicly available resources sixty-five new Microviridae prophages and episomes in bacterial genomes and retrieved forty-seven environmental viral genomes (EVGs) from various viromes. Genome clustering based on protein content and phylogenetic analysis showed that ICBM5, together with Rhizobium phages, new prophages, episomes, and EVGs cluster within two new phylogenetic clades, here tentatively assigned the rank of subfamily and named 'Tainavirinae' and 'Occultatumvirinae'. They both infect Rhodobacterales. Occultatumviruses also infect Hyphomicrobiales, including nitrogen-fixing endosymbionts from cosmopolitan legumes. A biogeographical assessment showed that tainaviruses and occultatumviruses are spread worldwide, in terrestrial and marine environments. The new phage isolated here sheds light onto new and diverse branches of the Microviridae tree, suggesting that much of the ssDNA phage diversity remains in the dark.

SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA