Voriconazole incorporated nanofiber formulations for topical application: preparation, characterization and antifungal activity studies against Candida species.

In this study, voriconazole (VCZ) incorporated polyvinyl alcohol/sodium alginate electrospun nanofibers were produced and, then crosslinked with glutaraldehyde for topical antifungal treatment. The nanofibers were characterized in terms of fiber size, surface morphology, and compatibility between drug-polymer and polymer-polymer using scanning electron microscopy, atomic force microscopy, attenuated total reflection-Fourier transform infrared spectroscopy, and high pressure liquid chromatography. After optimization studies, in vitro drug release, skin penetration, and deposition studies were performed using Franz diffusion cells. Antifungal activities of the nanofiber formulations against Candida albicans, Candida tropicalis, and Candida parapysilosis strains were evaluated using susceptibility test and subsequently time-kill study was performed against C. albicans. The cytotoxicity study was performed using 4-succinate dehydrogenase viability assay on mouse fibroblast cell line. The release rate of VCZ from crosslinked nanofibers was slower than that of non-crosslinked nanofibers and Higuchi kinetic model best fitted to the in vitro release data of both of formulations. VCZ deposited in deeper skin layers from nanofiber formulations was higher than that of the control formulation (VCZ solution in propylene glycol (1% (w/v)). According to the susceptibility and time-kill studies, all of the nanofiber formulations showed antifungal activity against C. albicans with confirming no cytotoxicity on mouse fibroblast cells.

The ameliorating approach of nanorobotics in the novel drug delivery systems: a mechanistic review.

Nanoscale robotics have the ability that it can productively transform multiple energy sources into motion and strength which reflects an expeditiously appearing and captivating area for research of robotics. In today's plethora, biomedical nanorobotics played an intricate character with numerous units of robots working at the pathological site in a coordinated manner. The synergistic action of the several nanorobotics has been employed for the fulfilment of the task such as large-scale detoxification, delivery of the large pharmacological/therapeutic efficacious payloads, etc. that is nearly unfeasible or unalterable practically by using single nanorobot. The collective intelligence of the nanorobot is advancing progressively at the nanoscale to reinforce their precision treatment potentially. Conclusively, after obtaining certain consideration regarding the nanorobotics sciences, many professionals are compendiously involving in the emerging highly efficacious therapeutic technology that encourages the scientist or designing of the tissues specific for the site-specific nanorobotic diagnostic devices. As a result, the closed and professional type between the field of Nanotechnology and Medical Sciences will provide another new highly oriented level to the domain of nanorobotics.

Preparation and characterization of naftifine-loaded poly(vinyl alcohol)/sodium alginate electrospun nanofibers

In this study, naftifine (a topical antifungal drug) loaded poly(vinyl) alcohol (PVA)/sodium alginate (SA) nanofibrous mats were prepared using the single-needle electrospinning technique. The produced nanofibers were crosslinked with glutaraldehyde (GTA) vapor. The morphology and diameter of the electrospun nanofibers were studied by scanning electron microscopy (SEM). SEM images showed the smoothness of the nanofibers and indicated that the fiber diameter increased with crosslinking and drug loading. Atomic force microscopy (AFM) images confirmed the uniform production of the scaffolds, and elemental mapping via energy dispersive X-ray spectroscopy (EDS) showed the uniform distribution of the drug within the nanofibers. An attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy study demonstrated that naftifine has sufficient secondary interactions with the polymer blend. The crosslinking treatment decreased the burst drug release effectively and the release mechanism followed Korsmeyer-Peppas Super Case-II transport. Overall, these findings suggest the potential use of naftifine-loaded PVA/SA nanofibers as a topical antifungal drug delivery system.