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The interruption of spinal circuitry following spinal cord injury (SCI) disrupts neural activity and is followed by a failure to mount an effective regenerative response resulting in permanent neurological disability. Functional recovery requires the enhancement of axonal and synaptic plasticity of spared as well as injured fibres, which need to sprout and/or regenerate to form new connections. Here, we have investigated whether the epigenetic stimulation of the regenerative gene expression program can overcome the current inability to promote neurological recovery in chronic SCI with severe disability. We delivered the CBP/p300 activator CSP-TTK21 or vehicle CSP weekly between week 12 and 22 following a transection model of SCI in mice housed in an enriched environment. Data analysis showed that CSP-TTK21 enhanced classical regenerative signalling in dorsal root ganglia sensory but not cortical motor neurons, stimulated motor and sensory axon growth, sprouting, and synaptic plasticity, but failed to promote neurological sensorimotor recovery. This work provides direct evidence that clinically suitable pharmacological CBP/p300 activation can promote the expression of regeneration-associated genes and axonal growth in a chronic SCI with severe neurological disability.
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Regeneración Nerviosa , Traumatismos de la Médula Espinal , Animales , Axones/metabolismo , Ratones , Regeneración Nerviosa/fisiología , Plasticidad Neuronal/fisiología , Recuperación de la Función/fisiología , Traumatismos de la Médula Espinal/metabolismoRESUMEN
Aging is the main risk factor of cognitive neurodegenerative diseases such as Alzheimer's disease, with epigenome alterations as a contributing factor. Here, we compared transcriptomic/epigenomic changes in the hippocampus, modified by aging and by tauopathy, an AD-related feature. We show that the cholesterol biosynthesis pathway is severely impaired in hippocampal neurons of tauopathic but not of aged mice pointing to vulnerability of these neurons in the disease. At the epigenomic level, histone hyperacetylation was observed at neuronal enhancers associated with glutamatergic regulations only in the tauopathy. Lastly, a treatment of tau mice with the CSP-TTK21 epi-drug that restored expression of key cholesterol biosynthesis genes counteracted hyperacetylation at neuronal enhancers and restored object memory. As acetyl-CoA is the primary substrate of both pathways, these data suggest that the rate of the cholesterol biosynthesis in hippocampal neurons may trigger epigenetic-driven changes, that may compromise the functions of hippocampal neurons in pathological conditions.
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Enfermedad de Alzheimer , Colesterol , Hipocampo , Ratones Transgénicos , Neuronas , Animales , Enfermedad de Alzheimer/metabolismo , Enfermedad de Alzheimer/genética , Hipocampo/metabolismo , Colesterol/biosíntesis , Colesterol/metabolismo , Neuronas/metabolismo , Ratones , Epigenómica , Epigénesis Genética , Ratones Endogámicos C57BL , Envejecimiento/metabolismo , Envejecimiento/genética , Masculino , Proteínas tau/metabolismo , Proteínas tau/genéticaRESUMEN
The development of robust electrocatalysts with low platinum content for acidic hydrogen evolution reaction (HER) is paramount for large scale commercialization of proton exchange membrane electrolyzers. Herein, a simple strategy is reported to synthesize a well anchored, low Pt containing Vulcan carbon catalyst using ZnO as a sacrificial template. Pt containing ZnO (PZ) is prepared by a simultaneous borohydride reduction. PZ is then loaded onto Vulcan carbon to produce a very low Pt content electrocatalyst, PZ@VC. PZ@VC with 2 wt.% Pt shows excellent performance for acidic HER in comparison to the commercial Pt/C (20 wt.%) catalyst. PZ@VC with a very low Pt loading shows significantly low η10 and η100 values (15 and 46 mV, respectively). PZ@VC on coating with Nafion (PZ@VC-N) shows further improvement in its performance (η10 of 7 mV, η100 of 28 mV) with ≈300 h of stability (≈10 mA cm-2 ) with only 4 µgPt cm-2 . PZ@VC-N shows a record high mass activity of 71 A mgPt -1 (32 times larger than Pt/C (20 wt.%) at 50 mV of overpotential. Post reaction characterizations reveal Pt nanoparticles are embedded onto VC with no traces of zinc, suggestive of a strong metal-support interaction leading to this high stability at low Pt loading.
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Aqueous electrochemical nitrogen reduction (ENR) to ammonia (NH3 ) under ambient conditions is considered as an alternative to the energy-intensive Haber-Bosch process for ammonia production. Many metal, non-metal, carbon-based materials along with metal-chalcogenides, metal-nitrides have been explored for their ENR activity. The reported NH3 production through ENR is still in the micro-gram level and often falls in the range of NH3 and NOx contaminations from the surrounding. The quantification of NH3 at very low concentration possess enormous challenge in this field and thus many reported ENR electrocatalysts suffer from reproducibility issue. This review highlights in detail the challenges associated with ENR in aqueous medium and necessitates standardization of protocols to quantify the low concentration of NH3 free of false-positives. It concludes the prospects of electrochemical NH3 production through lithium-mediated N2 reduction.
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Amoníaco , Nitrógeno , Reproducibilidad de los Resultados , CarbonoRESUMEN
Solution phase room-temperature phosphorescence (RTP) from organic phosphors is seldom realized. Herein we report one of the highest quantum yield solution state RTP (ca. 41.8 %) in water, from a structurally simple phthalimide phosphor, by employing an organic-inorganic supramolecular scaffolding strategy. We further use these supramolecular hybrid phosphors as a light-harvesting scaffold to achieve delayed fluorescence from orthogonally anchored Sulforhodamine acceptor dyes via an efficient triplet to singlet Förster resonance energy transfer (TS-FRET), which is rarely achieved in solution. Electrostatic cross-linking of the inorganic scaffold at higher concentrations further facilitates the formation of self-standing hydrogels with efficient RTP and energy-transfer mediated long-lived fluorescence.
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Sodium cobalt metaphosphate [NaCo(PO3 )3 ] has CoO octahedra (CoO6 ) and shows superior oxygen evolution reaction (OER) activity in alkaline solution, comparable with the state-of-the-art precious-metal RuO2 catalyst. OER catalysts of this metaphosphate are prepared by combustion (Cb) and solid-state (SS) methods. The combustion-assisted method offers a facile synthesis and one-step carbon composite formation. Unusually high catalytic activity was observed in NCoM-Cb-Ar and could be due to chemical coupling effects between NaCo(PO3 )3 and partially graphitized carbon. This novel electrocatalyst exhibits very small overpotential of 340â mV with high mass activity of 532â A g-1 . Good charge transfer abilities and chemical coupling between NaCo(PO3 )3 and amorphous carbon gives the OER activity in NCoM-Cb-Ar.
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Ambient solution and amorphous state room temperature phosphorescence (RTP) from purely organic chromophores is rarely achieved. Remarkable stabilization of triplet excitons is realized to obtain deep red phosphorescence in water and in amorphous film state under ambient conditions by a unique supramolecular hybrid assembly between inorganic laponite clay and heavy atom core substituted naphthalene diimide (NDI) phosphor. Structural rigidity and oxygen tolerance of the inorganic template along with controlled molecular organization via supramolecular scaffolding are envisaged to alleviate the unprecedented aqueous phase phosphorescence.
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Self-assembly of metal-organic framework (MOF) nanoparticles (NPs) with a functional material can result in MOF nanocomposites having new and advanced properties along with the fabrication of new nanoscopic structures. However, such assembly of MOFs has not been realized to date. Here we report self-assembled nanocomposites of the zeolitic imidazolate framework (ZIF-8) and layered aminoclay (AC) for the first time, and the ZIF-8@AC composites exhibit significantly enhanced adsorption properties in comparison to those of pristine ZIF-8 nanoparticles. Four different composites denoted as ZIF-8@AC-1, ZIF-8@AC-2, ZIF-8@AC-3, and ZIF-8@AC-4 were synthesized by varying the clay content, and their AC contents were found to be 12.1, 18.3, 22.2, and 27.2 wt %, respectively. The composites were thoroughly characterized by PXRD, FTIR, Raman, and various microscopic techniques (FESEM, TEM, and STEM). The formation of the composites is driven by the specific interaction between unsaturated Zn(II) sites of ZIF-8 nanoparticles and NH2 groups of the aminoclay, which was validated from ζ potential and Raman spectroscopic measurements. The adsorption studies of the desolvated composites were also carried out in detail. The best performance is achieved with one of the composites, which exhibits a 42% increase in BET surface area while CO2 uptake at 298 K is doubled in comparison to the ZIF-8 nanoparticles.
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An innovative technique to obtain high-surface-area mesostructured carbon (2545â m(2) g(-1)) with significant microporosity uses Teflon as the silica template removal agent. This method not only shortens synthesis time by combining silica removal and carbonization in a single step, but also assists in ultrafast removal of the template (in 10â min) with complete elimination of toxic HF usage. The obtained carbon material (JNC-1) displays excellent CO2 capture ability (ca. 26.2â wt % at 0 °C under 0.88â bar CO2 pressure), which is twice that of CMK-3 obtained by the HF etching method (13.0â wt %). JNC-1 demonstrated higher H2 adsorption capacity (2.8â wt %) compared to CMK-3 (1.2â wt %) at -196 °C under 1.0â bar H2 pressure. The bimodal pore architecture of JNC-1 led to superior supercapacitor performance, with a specific capacitance of 292â F g(-1) and 182â F g(-1) at a drain rate of 1â A g(-1) and 50â A g(-1) , respectively, in 1 m H2 SO4 compared to CMK-3 and activated carbon.
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Although the brain functions of specific acetyltransferases such as the CREB-binding protein (CBP) and p300 have been well documented using mutant transgenic mice models, studies based on their direct pharmacological activation are still missing due to the lack of cell-permeable activators. Here we present a small-molecule (TTK21) activator of the histone acetyltransferases CBP/p300, which, when conjugated to glucose-based carbon nanosphere (CSP), passed the blood-brain barrier, induced no toxicity, and reached different parts of the brain. After intraperitoneal administration in mice, CSP-TTK21 significantly acetylated histones in the hippocampus and frontal cortex. Remarkably, CSP-TTK21 treatment promoted the formation of long and highly branched doublecortin-positive neurons in the subgranular zone of the dentate gyrus and reduced BrdU incorporation, suggesting that CBP/p300 activation favors maturation and differentiation of adult neuronal progenitors. In addition, mRNA levels of the neuroD1 differentiation marker and BDNF, a neurotrophin required for the terminal differentiation of newly generated neurons, were both increased in the hippocampus concomitantly with an enrichment of acetylated-histone on their proximal promoter. Finally, we found that CBP/p300 activation during a spatial training, while not improving retention of a recent memory, resulted in a significant extension of memory duration. This report is the first evidence for CBP/p300-mediated histone acetylation in the brain by an activator molecule, which has beneficial implications for the brain functions of adult neurogenesis and long-term memory. We propose that direct stimulation of acetyltransferase function could be useful in terms of therapeutic options for brain diseases.
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Proteína de Unión a CREB/metabolismo , Activadores de Enzimas/farmacología , Memoria/efectos de los fármacos , Neurogénesis/efectos de los fármacos , Factores de Transcripción p300-CBP/metabolismo , Acetiltransferasas/metabolismo , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo , Encéfalo/crecimiento & desarrollo , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Recuento de Células , Núcleo Celular/metabolismo , Inmunoprecipitación de Cromatina , Dendritas/metabolismo , Dendritas/ultraestructura , Técnica del Anticuerpo Fluorescente , Hipocampo/citología , Hipocampo/metabolismo , Histona Acetiltransferasas/metabolismo , Histonas/aislamiento & purificación , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos C57BL , Nanosferas , Neuronas/metabolismo , Neuronas/ultraestructura , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
Several covalent strategies towards surface charge-reversal in nanochannels have been reported with the purpose of manipulating ion transport. However, covalent routes lack dynamism, modularity and post-synthetic flexibility, and hence restrict their applicability in different environments. Here, we introduce a facile non-covalent approach towards charge-reversal in nanochannels (<10â nm) using strong charge-transfer interactions between dicationic viologen (acceptor) and trianionic pyranine (donor). The polarity of ion transport was switched from anion selective to ambipolar to cation selective by controlling the extent of viologen bound to the pyranine. We could also regulate the ion transport with respect to pH by selecting a donor with pH-responsive functional groups. The modularity of this approach further allows facile integration of various functional groups capable of responding to stimuli such as light and temperature to modulate the transport of ions as well as molecules.
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Nanoestructuras/química , Transporte Iónico , Electrodos de Iones SelectosRESUMEN
The electrochemical reduction of nitrate (NO3-) ions to ammonia (NH3) provides an alternative method to eliminate harmful NO3- pollutants in water as well as to produce highly valuable NH3 chemicals. The NH3 yield rate however is still limited to the µmol h-1 cm-2 range when dealing with dilute NO3- concentrations found in waste streams. Copper (Cu) has attracted much attention because of its unique ability to effectively convert NO3- to NH3. We have reported a simple and scalable electrochemical method to produce a Cu foil having its surface covered with a porous Cu nanostructure enriched with (100) facets, which efficiently catalyzes NO3- to NH3. The Cu(100)-rich electrocatalyst showed a very high NH3 production rate of 1.1 mmol h-1 cm-2 in NO3- concentration as low as 14 mM NO3-, which is 4-5 times higher than the best-reported values. Increasing the NO3- concentration (140 mM) resulted in an NH3 production yield rate of 3.34 mmol h-1 cm-2. The durability test conducted for this catalyst foil in a flow cell system showed greater than 100 h stability with a Faradaic efficiency greater than 98%, demonstrating its potential to be used on an industrially relevant scale. Further, density functional theory (DFT) calculations have been performed to understand the better catalytic activity of Cu(100) compared to Cu(111) facets toward NO3-RR.
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Light-harvesting hybrids have gained much importance as they are considered as potential mimics for photosynthetic systems. In this Concept article we introduce the design concepts involved in the building up of light-harvesting hybrids; these resemble the well-studied organic-based assemblies for energy transfer. We have structured this article into three parts based on the strategies adopted in the synthesis of hybrid assemblies, as covalent, semicovalent, and noncovalent procedures. Furthermore, the properties and structural features of the hybrids and analogous organic assemblies are compared. We also emphasize the challenges involved in the processability of these hybrid materials for device applications and present our views and results to address this issue through the design of soft-hybrids by a solution-state, noncovalent, self-assembly process.
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BACKGROUND: Intrinsically fluorescent glucose derived carbon nanospheres (CSP) efficiently enter mammalian cells and also cross the blood brain barrier (BBB). However, the mechanistic details of CSP entry inside mammalian cells and its specificity are not known. RESULTS: In this report, the biochemical and cellular mechanism of CSP entry into the living cell have been investigated. By employing confocal imaging we show that CSP entry into the mammalian cells is an ATP-dependent clathrin mediated endocytosis process. Zeta potential studies suggest that it has a strong preference for cells which possess high levels of glucose transporters such as the glial cells, thereby enabling it to target individual organs/tissues such as the brain with increased specificity. CONCLUSION: The endocytosis of Glucose derived CSP into mammalian cells is an ATP dependent process mediated by clathrin coated pits. CSPs utilize the surface functional groups to target cells containing glucose transporters on its membrane thereby implicating a potential application for specific targeting of the brain or cancer cells.
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Adenosina Trifosfato/metabolismo , Carbono/farmacocinética , Clatrina/metabolismo , Nanosferas/química , Receptores de Superficie Celular/metabolismo , Animales , Encéfalo/metabolismo , Química Encefálica , Carbono/química , Línea Celular Tumoral , Desoxiglucosa/metabolismo , Endocitosis , Células HeLa , Humanos , Ratones , Ratones Endogámicos BALB C , Microscopía Confocal , Células 3T3 NIH , Sacarosa , TemperaturaRESUMEN
Synthesis of ammonia through electrochemical nitrogen reduction (ENR) is emerging as one of the attractive research areas in recent years, notwithstanding the enormous challenges it faces in quantification of ammonia at very low concentrations. Several reports claiming high production rate are unwittingly compromised by the accuracy of analyzing a very low concentration (<1 ppm) of ammonia in the electrolyte post-ENR reaction using the indophenol method. Therefore, in this work, we have highlighted the significance of selecting and standardizing a right protocol encompassing admissible levels of oxidants and a complexing agent, citrate (to mitigate the effect of interfering metal ions), through elaborate control experiments. In addition, the importance of setting the lowest limit of ammonia concentration that can be accurately quantified by the indophenol method is also justified. Further, the experimental observations were summarized into a protocol, which was followed to re-evaluate the performance of two well-claimed electrocatalysts for ENR reported recently in the literature.
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NiFe layered double hydroxide (NiFe LDH) grown in the presence of MoS2 (rich in 1T phase) shows exceptional performance metrics for alkaline oxygen evolution reaction (OER) in this class of composites. The as-prepared NiFe LDH/MoS2 composite (abbreviated as MNF) exhibits a low overpotential (η10) of 190 mV; a low Tafel slope of 31 mV dec-1; and more importantly, a high stability in its performance manifested by the delivery of current output for 45 h. It is important to note that this could be achieved with an exceedingly low loading of 0.14 mg cm-2. The mass activity of this composite (97 A g-1) is about 14 times greater than that of the conventional RuO2 (7 A g-1) at η = 200 mV. When normalized with respect to the total metal content, a mass activity of 1000 A g-1 (η = 300 mV) was achieved. Impedance analysis further reveals that the significant reduction in charge-transfer resistance and hence high current density (5 times greater as compared to NiFe LDH at η = 300 mV) observed for MNF is associated with interfacial adsorption kinetics of intermediates (R1). Significant enhancement in the intrinsic activity of MNF over LDH has been observed through normalization of current with the electrochemically active surface area. Computational studies suggest that the Ni centers in the composite act as the active sites for OER, which is well-corroborated with the observed postreaction appearance of Ni3+ species.
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The master epigenetic regulator lysine acetyltransferase (KAT) p300/CBP plays a pivotal role in neuroplasticity and cognitive functions. Recent evidence has shown that in several neurodegenerative diseases, including Alzheimer's disease (AD), the expression level and function of p300/CBP are severely compromised, leading to altered gene expression causing pathological conditions. Here, we show that p300/CBP activation by a small-molecule TTK21, conjugated to carbon nanosphere (CSP) ameliorates Aß-impaired long-term potentiation (LTP) induced by high-frequency stimulation, theta burst stimulation, and synaptic tagging/capture (STC). This functional rescue was correlated with CSP-TTK21-induced changes in transcription and translation. Mechanistically, we observed that the expression of a large number of synaptic plasticity- and memory-related genes was rescued, presumably by the restoration of p300/CBP mediated acetylation. Collectively, these results suggest that small-molecule activators of p300/CBP could be a potential therapeutic molecule for neurodegenerative diseases like AD.
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Nanosferas , Acetilación , Acetiltransferasas/metabolismo , Carbono/metabolismo , Glucosa/metabolismo , Hipocampo/metabolismo , Histonas/metabolismo , Células Piramidales/metabolismoRESUMEN
Existing phenol production processes tend to be energy-consuming and produce unwanted by-products. We report an efficient process using a shell-and-tube reactor, in which a gaseous mixture of benzene and oxygen is fed into a porous alumina tube coated with a palladium thin layer and hydrogen is fed into the shell. Hydrogen dissociated on the palladium layer surface permeates onto the back and reacts with oxygen to give active oxygen species, which attack benzene to produce phenol. This one-step process attained phenol formation selectivities of 80 to 97% at benzene conversions of 2 to 16% below 250 degrees C (phenol yield: 1.5 kilograms per kilogram of catalyst per hour at 150 degrees C).
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Temporal regulation of mass transport across the membrane is a vital feature of biological systems. Such regulatory mechanisms rely on complex biochemical reaction networks, often operating far from equilibrium. Herein, we demonstrate biochemical reaction mediated temporal regulation of mass transport in nanochannels of mesoporous silica sphere. The rationally designed nanochannels with pH responsive electrostatic gating are fabricated through a hetero-functionalization approach utilizing propylamine and carboxylic acid moieties. At basic pH, cationic small molecules can diffuse into the nanochannels which release back to the solution at acidic pH. The transient ion transport is temporally controlled using a base as fuel along with esterase enzyme as the mediator. The slow enzymatic hydrolysis of a dormant deactivator (ethyl acetate) determines the lifetime of transient encapsulated state, which can be programmed easily by modulating the enzymatic activity of esterase. This system represents a unique approach to create autonomous artificial cellular models.
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Glioblastoma multiforme (GBM), the highly invasive form of glioma, exhibits the highest mortality in patients with brain malignancies. Increasing glioma patients' survivability is challenging, as targeting only tumor-associated malignant cells would not reduce the overall aggressiveness of the tumor mass. This is due to the inadequacy in countering pro-proliferative, invasive and metastatic factors released by tumor-mass associated macrophages (TAMs). Hence, strategically, dual targeting both tumor cells and TAMs is necessary for effective glioma treatment and increased survivability. Conventional FR-targeting systems can easily target cancer cells that overtly express folate receptors (FRs). However, FRs are expressed only moderately in both glioma cells and in TAMs. Hence, it is more challenging to coordinate dual targeting of glioma cells and TAMs with lower levels of FR expression. A recently developed carbon nanosphere (CSP) with effective blood-brain barrier (BBB) penetrability was modified with a new folic acid-cationic lipid conjugate (F8) as a targeting ligand. The uniqueness of the cationic lipid-folate conjugate is that it stably associates with the negatively charged CSP surface at about >22 mol% surface concentration, a concentration at least 5-fold higher than what is achieved for conventional FR-targeting delivery systems. This enabled dual uptake of the CSP on TAMs and tumor cells via FRs. A doxorubicin-associated FR-targeting formulation (CFD), in an orthotopic glioma model and in a glioma subcutaneous model, induced the maximum anticancer effect with enhanced average mice survivability twice that of untreated mice and without any systemic liver toxicity. Additionally, we observed a significant decrease of TAM-released pro-aggressive factors, TGF-ß, STAT3, invasion and migration related sICAM-1, and other cytokines indicating anti-TAM activity of the CFD. Taken together, we principally devised, to the best of our knowledge, the first FR-targeting nano-delivery system for targeting brain-associated TAMs and tumor cells as an efficient glioma therapeutic.