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1.
Nanotechnology ; 33(43)2022 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-35830770

RESUMEN

Antimicrobial peptides (AMPs) and lipopeptides (LPs) represent very promising molecules to fight resistant bacterial infections due to their broad-spectrum of activity, their first target, i.e. the bacterial membrane, and the rapid bactericidal action. For both types of molecules, the action mechanism starts from the membrane of the pathogen agents, producing a disorganization of their phase structure or the formation of pores of different size altering their permeability. This mechanism of action is based on physical interactions more than on a lock-and-key recognition event and it is difficult for the pathogens to rapidly develop an effective resistance. Very small differences in the sequence of both AMPs and LPs might lead to very different effects on the target membrane. Therefore, a correct understanding of their mechanism of action is required with the aim of developing new synthetic peptides, analogues of the natural ones, with specific and more powerful bactericidal activity. Atomic force microscopy (AFM), with its high resolution and the associated force spectroscopy resource, provides a valuable technique to investigate the reorganization of lipid bilayers exposed to antimicrobial or lipopeptides. Here, we present AFM results obtained by ours and other groups on the action of AMPs and LPs on supported lipid bilayers (SLBs) of different composition. We also consider data obtained by fluorescence microscopy to compare the AFM data with another technique which can be used on different lipid bilayer model systems such as SLBs and giant unilamellar vesicles. The outcomes here presented highlight the powerful of AFM-based techniques in detecting nanoscale peptide-membrane interactions and strengthen their use as an exceptional complementary tool toin vivoinvestigations. Indeed, the combination of these approaches can help decipher the mechanisms of action of different antimicrobials and lipopeptides at both the micro and nanoscale levels, and to design new and more efficient antimicrobial compounds.


Asunto(s)
Antiinfecciosos , Membrana Dobles de Lípidos , Antibacterianos/química , Membrana Dobles de Lípidos/química , Lipopéptidos/farmacología , Lipopolisacáridos , Microscopía de Fuerza Atómica/métodos
2.
Sensors (Basel) ; 21(9)2021 Apr 27.
Artículo en Inglés | MEDLINE | ID: mdl-33925730

RESUMEN

Nucleic acid (NA) extraction is a basic step for genetic analysis, from scientific research to diagnostic and forensic applications. It aims at preparing samples for its application with biomolecular technologies such as isothermal and non-isothermal amplification, hybridization, electrophoresis, Sanger sequencing and next-generation sequencing. Multiple steps are involved in NA collection from raw samples, including cell separation from the rest of the specimen, cell lysis, NA isolation and release. Typically, this process needs molecular biology facilities, specialized instrumentation and labor-intensive operations. Microfluidic devices have been developed to analyze NA samples with high efficacy and sensitivity. In this context, the integration within the chip of the sample preparation phase is crucial to leverage the promise of portable, fast, user-friendly and economic point-of-care solutions. This review presents an overview of existing lab-on-a-chip (LOC) solutions designed to provide automated NA extraction from human raw biological fluids, such as whole blood, excreta (urine and feces), saliva. It mainly focuses on LOC implementation aspects, aiming to describe a detailed panorama of strategies implemented for different human raw sample preparations.


Asunto(s)
Técnicas Analíticas Microfluídicas , Ácidos Nucleicos , Humanos , Dispositivos Laboratorio en un Chip , Microfluídica , Técnicas de Amplificación de Ácido Nucleico , Sistemas de Atención de Punto
3.
Biophys J ; 116(3): 503-517, 2019 02 05.
Artículo en Inglés | MEDLINE | ID: mdl-30665697

RESUMEN

Understanding the lateral organization of biological membranes plays a key role on the road to fully appreciate the physiological functions of this fundamental barrier between the inside and outside regions of a cell. Ternary lipid bilayers composed of a high and a low melting temperature lipid and cholesterol represent a model system that mimics some of the important thermodynamical features of much more complex lipid mixtures such as those found in mammal membranes. The phase diagram of these ternary mixtures can be studied exploiting fluorescence microscopy in giant unilamellar vesicles, and it is typically expected to give rise, for specific combinations of composition and temperature, to regions of two-phase coexistence and a region with three-phase coexistence, namely, the liquid-ordered, liquid-disordered, and solid phases. Whereas the observation of two-phase coexistence is routinely possible using fluorescence microscopy, the three-phase region is more elusive to study. In this article, we show that particular lipid mixtures containing diphytanoyl-phosphatidylcholine and cholesterol plus different types of sphingomyelin (SM) are prone to produce bilayer regions with more than two levels of fluorescence intensity. We found that these intensity levels occur at low temperature and are linked to the copresence of long and asymmetric chains in SMs and diphytanoyl-phosphatidylcholine in the lipid mixtures. We discuss the possible interpretations for this observation in terms of bilayer phase organization in the presence of sphingolipids. Additionally, we also show that in some cases, liposomes in the three-phase coexistence state exhibit extreme sensitivity to lateral tension. We hypothesize that the appearance of the different phases is related to the asymmetric structure of SMs and to interdigitation effects.


Asunto(s)
Esfingomielinas/química , Liposomas Unilamelares/química , Colesterol/química , Estrés Mecánico , Temperatura
4.
Biochim Biophys Acta ; 1848(5): 1258-67, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-25620773

RESUMEN

Amphiphilic molecules which have a biological effect on specific membrane proteins, could also affect lipid bilayer properties possibly resulting in a modulation of the overall membrane behavior. In light of this consideration, it is important to study the possible effects of amphiphilic molecule of pharmacological interest on model systems which recapitulate some of the main properties of the biological plasma membranes. In this work we studied the effect of a neurosteroid, Allopregnanolone (3α,5α-tetrahydroprogesterone or Allo), on a model bilayer composed by the ternary lipid mixture DOPC/bSM/chol. We chose ternary mixtures which present, at room temperature, a phase coexistence of liquid ordered (Lo) and liquid disordered (Ld) domains and which reside near to a critical point. We found that Allo, which is able to strongly partition in the lipid bilayer, induces a marked increase in the bilayer area and modifies the relative proportion of the two phases favoring the Ld phase. We also found that the neurosteroid shifts the miscibility temperature to higher values in a way similarly to what happens when the cholesterol concentration is decreased. Interestingly, an isoform of Allo, isoAllopregnanolone (3ß,5α-tetrahydroprogesterone or isoAllo), known to inhibit the effects of Allo on GABAA receptors, has an opposite effect on the bilayer properties.


Asunto(s)
Colesterol/química , Membrana Dobles de Lípidos , Microscopía de Fuerza Atómica , Neurotransmisores/química , Fosfatidilcolinas/química , Pregnanolona/química , Esfingomielinas/química , Tensoactivos/química , Isomerismo , Neurotransmisores/farmacología , Pregnanolona/farmacología , Tensoactivos/farmacología , Temperatura de Transición
5.
Biochim Biophys Acta ; 1848(5): 1268-76, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-25660752

RESUMEN

Amphiphilic molecules supposed to affect membrane protein activity could strongly interact also with the lipid component of the membrane itself. Neurosteroids are amphiphilic molecules that bind to plasma membrane receptors of cells in the central nervous system but their effect on membrane is still under debate. For this reason it is interesting to investigate their effects on pure lipid bilayers as model systems. Using the micropipette aspiration technique (MAT), here we studied the effects of a neurosteroid, allopregnanolone (3α,5α-tetrahydroprogesterone or Allo) and of one of its isoforms, isoallopregnanolone (3ß,5α-tetrahydroprogesterone or isoAllo), on the physical properties of pure lipid bilayers composed by DOPC/bSM/chol. Allo is a well-known positive allosteric modulator of GABAA receptor activity while isoAllo acts as a non-competitive functional antagonist of Allo modulation. We found that Allo, when applied at nanomolar concentrations (50-200 nM) to a lipid bilayer model system including cholesterol, induces an increase of the lipid bilayer area and a decrease of the mechanical parameters. Conversely, isoAllo, decreases the lipid bilayer area and, when applied, at the same nanomolar concentrations, it does not affect significantly its mechanical parameters. We characterized the kinetics of Allo uptake by the lipid bilayer and we also discussed its aspects in relation to the slow kinetics of Allo gating effects on GABAA receptors. The overall results presented here show that a correlation exists between the modulation of Allo and isoAllo of GABAA receptor activity and their effects on a lipid bilayer model system containing cholesterol.


Asunto(s)
Colesterol/química , Membranas Artificiales , Neurotransmisores/química , Fosfatidilcolinas/química , Pregnanolona/química , Esfingomielinas/química , Tensoactivos/química , Colesterol/metabolismo , Isomerismo , Cinética , Neurotransmisores/metabolismo , Neurotransmisores/farmacología , Fosfatidilcolinas/metabolismo , Pregnanolona/metabolismo , Pregnanolona/farmacología , Receptores de GABA-A/química , Receptores de GABA-A/efectos de los fármacos , Receptores de GABA-A/metabolismo , Esfingomielinas/metabolismo , Succión , Tensoactivos/metabolismo , Tensoactivos/farmacología
6.
Biochim Biophys Acta ; 1828(2): 405-11, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-23036932

RESUMEN

γ-Hemolysins are bicomponent ß-barrel pore forming toxins produced by Staphylococcus aureus as water-soluble monomers, which assemble into oligomeric pores on the surface of lipid bilayers. Here, after investigating the oligomeric structure of γ-hemolysins on supported lipid bilayers (SLBs) by atomic force microscopy (AFM), we studied the effect produced by this toxin on the structure of SLBs. We found that oligomeric structures with different number of monomers can assemble on the lipid bilayer being the octameric form the stablest one. Moreover, in this membrane model we found that γ-hemolysins can form clusters of oligomers inducing a curvature in the lipid bilayer, which could probably enhance the aggressiveness of these toxins at high concentrations.


Asunto(s)
Proteínas Hemolisinas/química , Membrana Dobles de Lípidos/química , Microscopía de Fuerza Atómica/métodos , Staphylococcus aureus/metabolismo , Proteínas Bacterianas/química , Biofisica/métodos , Cristalografía por Rayos X/métodos , Transferencia Resonante de Energía de Fluorescencia/métodos , Procesamiento de Imagen Asistido por Computador , Lípidos/química , Liposomas/química , Modelos Moleculares , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Factores de Tiempo
7.
Soft Matter ; 10(37): 7145-64, 2014 Oct 07.
Artículo en Inglés | MEDLINE | ID: mdl-25090108

RESUMEN

We review the capabilities of Atomic Force Microscopy (AFM) in the study of phase transitions in Supported Lipid Bilayers (SLBs). AFM represents a powerful technique to cover the resolution range not available to fluorescence imaging techniques and where spectroscopic data suggest what the relevant lateral scale for domain formation might be. Phase transitions of lipid bilayers involve the formation of domains characterized by different heights with respect to the surrounding phase and are therefore easily identified by AFM in liquid solution once the bilayer is confined to a flat surface. Even if not endowed with high time resolution, AFM allows light to be shed on some aspects related to lipid phase transitions in the case of both a single lipid component and lipid mixtures containing sterols also. We discuss here the obtained results in light of the peculiarities of supported lipid bilayer model systems.


Asunto(s)
Membrana Dobles de Lípidos/química , Lípidos/química , Microscopía de Fuerza Atómica/métodos , Transición de Fase , Difusión , Concentración de Iones de Hidrógeno , Iones , Cinética , Ensayo de Materiales , Fosfatidiletanolaminas/química , Fosfatidilgliceroles/química , Fosfolípidos/química , Electricidad Estática , Temperatura , Termodinámica
8.
Biophys J ; 103(1): 38-47, 2012 Jul 03.
Artículo en Inglés | MEDLINE | ID: mdl-22828330

RESUMEN

Biological membranes are constantly exposed to forces. The stress-strain relation in membranes determines the behavior of many integral membrane proteins or other membrane related-proteins that show a mechanosensitive behavior. Here, we studied by force spectroscopy the behavior of supported lipid bilayers (SLBs) subjected to forces perpendicular to their plane. We measured the lipid bilayer mechanical properties and the force required for the punch-through event characteristic of atomic force spectroscopy on SLBs as a function of the interleaflet coupling. We found that for an uncoupled bilayer, the overall tip penetration occurs sequentially through the two leaflets, giving rise to two penetration events. In the case of a bilayer with coupled leaflets, penetration of the atomic force microscope tip always occurred in a single step. Considering the dependence of the jump-through force value on the tip speed, we also studied the process in the context of dynamic force spectroscopy (DFS). We performed DFS experiments by changing the temperature and cantilever spring constant, and analyzed the results in the context of the developed theories for DFS. We found that experiments performed at different temperatures and with different cantilever spring constants enabled a more effective comparison of experimental data with theory in comparison with previously published data.


Asunto(s)
Membrana Dobles de Lípidos/química , Microscopía de Fuerza Atómica/métodos , Temperatura
9.
J Mol Recognit ; 24(3): 387-96, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-21504015

RESUMEN

The current view of the biological membrane is that in which lipids and proteins mutually interact to accomplish membrane functions. The lateral heterogeneity of the lipid bilayer can induce partitioning of membrane-associated proteins, favoring protein-protein interaction and influence signaling and trafficking. The Atomic Force Microscope allows to study the localization of membrane-associated proteins with respect to the lipid organization at the single molecule level and without the need for fluorescence staining. These features make AFM a technique of choice to study lipid/protein interactions in model systems or native membranes. Here we will review the technical aspects inherent to and the main results obtained by AFM in the study of protein partitioning in lipid domains concentrating in particular on GPI-anchored proteins, lipidated proteins, and transmembrane proteins. Whenever possible, we will also discuss the functional consequences of what has been imaged by Atomic Force Microscopy.


Asunto(s)
Membrana Dobles de Lípidos/química , Lípidos/química , Microscopía de Fuerza Atómica/métodos , Proteínas/química , Unión Proteica
10.
Chem Res Toxicol ; 24(8): 1283-96, 2011 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-21728342

RESUMEN

The cytolytic action of palytoxin (PlTX) was recognized long ago, but its features have remained largely undetermined. We used biochemical, morphological, physiological, and physical tools, to study the cytolytic response in MCF-7 cells, as our model system. Cytolysis represented a stereotyped response induced by the addition of isotonic phosphate buffer (PBS) to cells that had been exposed to PlTX, after toxin removal and under optimal and suboptimal experimental conditions. Cytolysis was sensitive to osmolytes present during cell exposure to PlTX but not in the course of the lytic phase. Fluorescence microscopy showed that PlTX caused cell rounding and rearrangement of the actin cytoskeleton. Atomic force microscopy (AFM) was used to monitor PlTX effects in real time, and we found that morphological and mechanical properties of MCF-7 cells did not change during toxin exposure, but increased cell height and decreased stiffness at its surface were observed when PBS was added to PlTX-treated cells. The presence of an osmolyte during PlTX treatment prevented the detection of changes in morphological and mechanical properties caused by PBS addition to toxin-treated cells, as detected by AFM. By patch-clamp technique, we confirmed that PlTX action involved the transformation of the Na(+),K(+)-ATPase into a channel and found that cell membrane capacitance was not changed by PlTX, indicating that the membrane surface area was not greatly affected in our model system. Overall, our findings show that the cytolytic response triggered by PlTX in MCF-7 cells includes a first phase, which is toxin-dependent and osmolyte-sensitive, priming cells to lytic events taking place in a separate phase, which does not require the presence of the toxin and is osmolyte-insensitive but is accompanied by marked reorganization of actin-based cytoskeleton and altered mechanical properties at the cell's surface. A model of the two-step process of PlTX-induced cytolysis is presented.


Asunto(s)
Acrilamidas/toxicidad , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Venenos de Cnidarios , Humanos , Microscopía de Fuerza Atómica , Microscopía Fluorescente , Concentración Osmolar , Técnicas de Placa-Clamp , ATPasa Intercambiadora de Sodio-Potasio/metabolismo
11.
Biophys J ; 99(11): 3675-83, 2010 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-21112292

RESUMEN

We show that the activity of an ion channel is correlated with the phase state of the lipid bilayer hosting the channel. By measuring unitary conductance, dwell times, and open probability of the K(+) channel KcsA as a function of temperature in lipid bilayers composed of POPE and POPG in different relative proportions, we obtain that all those properties show a trend inversion when the bilayer is in the transition region between the liquid-disordered and the solid-ordered phase. These data suggest that the physical properties of the lipid bilayer influence ion channel activity likely via a fine-tuning of its conformations. In a more general interpretative framework, we suggest that other parameters such as pH, ionic strength, and the action of amphiphilic drugs can affect the physical behavior of the lipid bilayer in a fashion similar to temperature changes resulting in functional changes of transmembrane proteins.


Asunto(s)
Proteínas Bacterianas/metabolismo , Activación del Canal Iónico/fisiología , Transición de Fase , Fosfatidiletanolaminas/química , Fosfatidilgliceroles/química , Canales de Potasio/metabolismo , Streptomyces lividans/metabolismo , Conductividad Eléctrica , Membrana Dobles de Lípidos/química , Temperatura , Factores de Tiempo
12.
J Am Chem Soc ; 132(21): 7445-53, 2010 Jun 02.
Artículo en Inglés | MEDLINE | ID: mdl-20455538

RESUMEN

The hydroquinone/benzoquinone redox couple involves the exchange of two electrons and two protons in its oxidation/reduction reaction in aqueous buffered solutions. In this work, we employed Electrochemical Scanning Tunneling Microscopy and Spectroscopy (EC-STM, EC-STS) to study the interfacial electron transfer properties of hydroquinone incorporated in a Self Assembled Monolayer on a Au(111) substrate. The exchange of electrons between the STM tip and the substrate is regulated by the redox levels of the sandwiched molecule and showed the presence of two regions of tunneling enhancement in the tunneling current/overvoltage relationship. The two regions can be attributed to the presence of two one-electron transfer processes whose equilibrium positions shift upon pH variations. This is the first time a redox molecule involving the exchange of both electrons and protons is studied by EC-STM and EC-STS. The hydroquinone/benzoquinone redox couple can be exploited to obtain an electrochemically or a pH gated transistor.

13.
Nanotechnology ; 20(23): 235101, 2009 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-19448298

RESUMEN

Functional DNA scaffolds can be defined as DNA-based structures comprising chemical moieties facilitating and guiding the immobilization of additional nanocomponents. Due to the limited reactivity of DNA there is currently a need to develop rapid routes to expand its chemical repertoire and increase its versatility as a nanostructuring scaffold. We report a simple synthetic strategy for generating linear and stable double-stranded DNA scaffolds functionalized with multiple sites reactive towards free thiols, and the utility of this approach is demonstrated by immobilizing a model protein containing an accessible free thiol. This procedure is very versatile and could be easily expanded to other types of chemistries. This approach could also potentially be employed for the specific, oriented immobilization of various biomolecules and nanoparticles on predefined DNA architectures.


Asunto(s)
ADN/química , Proteínas Inmovilizadas/química , Nanoestructuras/química , Nanotecnología/métodos , Compuestos Alílicos/química , Compuestos Alílicos/metabolismo , Citocromos c/química , Citocromos c/metabolismo , ADN/metabolismo , Proteínas Inmovilizadas/metabolismo , Microscopía de Fuerza Atómica , Modelos Químicos , Nanoestructuras/ultraestructura , Compuestos de Sulfhidrilo/química , Compuestos de Sulfhidrilo/metabolismo , Uridina Trifosfato/análogos & derivados , Uridina Trifosfato/química , Uridina Trifosfato/metabolismo
14.
Anal Chim Acta ; 1087: 121-130, 2019 Dec 09.
Artículo en Inglés | MEDLINE | ID: mdl-31585560

RESUMEN

Protein misfolding and aggregation are the common mechanisms in a variety of aggregation-dependent diseases. The compromised proteins often assemble into toxic, accumulating amyloid-like structures of various lengths and their toxicity can also be transferred both in vivo and in vitro a prion-like behavior. The characterization of protein interactions, degradation and conformational dynamics in biological systems still represents an analytical challenge in the prion-like protein comprehension. In our work, we investigated the nature of a transferable cytotoxic agent, presumably a misfolded protein, through the coupling of a multi-detector, non-destructive separation platform based on hollow-fiber flow field-flow fractionation with imaging and downstream in vitro tests. After purification with ion exchange chromatography, the transferable cytotoxic agentwas analyzed with Atomic Force Microscopy and statistical analysis, showing that the concentration of protein dimers and low n-oligomer forms was higher in the cytotoxic sample than in the control preparation. To assess whether the presence of these species was the actual toxic and/or self-propagating factor, we employed HF5 fractionation, with UV and Multi-Angle Light Scattering detection, to define proteins molar mass distribution and abundance, and fractionate the sample into size-homogeneous fractions. These fractions were then tested individually in vitro to investigate the direct correlation with cytotoxicity. Only the later-eluted fraction, which contains high-molar mass aggregates, proved to be toxic onto cell cultures. Moreover, it was observed that the selective transfer of toxicity also occurs for one lower-mass fraction, suggesting that two different mechanisms, acute and later induced toxicity, are in place. These results strongly encourage the efficacy of this platform to enable the identification of protein toxicants.


Asunto(s)
Proteínas Amiloidogénicas/análisis , Priones/análisis , Agregado de Proteínas , Proteínas Amiloidogénicas/aislamiento & purificación , Proteínas Amiloidogénicas/toxicidad , Línea Celular Tumoral , Cromatografía por Intercambio Iónico , Fraccionamiento de Campo-Flujo , Humanos , Luz , Microscopía de Fuerza Atómica , Tamaño de la Partícula , Priones/aislamiento & purificación , Priones/toxicidad , Dispersión de Radiación
15.
Microsc Res Tech ; 71(4): 274-8, 2008 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-18058826

RESUMEN

We report on a novel use of electrochemical scanning force microscopy (SFM) for the investigation of morphological modifications occurring in plasma membranes containing voltage-gated ion channels, on membrane potential variation. Membrane patches of Xenopus laevis oocytes microinjected with exogenous KAT1 cRNA, deposited by a stripping method at the surface of a derivatized gold film in inside-out configuration, have been imaged by SFM in an electrochemical cell. A potentiostat was used to maintain a desired potential drop across the membrane. Performing imaging at potential values corresponding to open (-120 mV) and closed (+20 mV) states for KAT1, morphological differences in localized sample zones were observed. Particularly, cross-shaped features involving a significant membrane portion appear around putative channel locations. The reported approach constitutes the first demonstration of an SPM-based experimental technique suitable to investigate the rearrangements occurring to the plasma membrane containing voltage-gated channels on transmembrane potential variation.


Asunto(s)
Proteínas de Arabidopsis/metabolismo , Membrana Celular , Activación del Canal Iónico , Microscopía de Fuerza Atómica/métodos , Oocitos , Canales de Potasio de Rectificación Interna/metabolismo , Animales , Proteínas de Arabidopsis/genética , Membrana Celular/metabolismo , Membrana Celular/ultraestructura , Conductividad Eléctrica , Electroquímica , Oocitos/metabolismo , Oocitos/ultraestructura , Canales de Potasio de Rectificación Interna/genética , Canales de Potasio con Entrada de Voltaje , Xenopus laevis
16.
Biophys Chem ; 229: 39-45, 2017 10.
Artículo en Inglés | MEDLINE | ID: mdl-28818313

RESUMEN

An innovative platform for the study of the molecular mechanisms at the basis of mechanotransduction has been implemented, developing an experimental approach capable of providing controlled dynamic compression stimuli and retrieving the biomolecular response with single-cell sensitivity. The system provides the ability to perform compression-release cycles on single cells with controlled forces in the nN range and a user-defined repetition rate. Experimental procedures to perform qPCR from a small set of single cells were finely tuned. The experimental platform was tested in the context of bone (cell line hFOB 1.19), a physiological environment highly subjected to mechanical stimuli. Target genes were identified in the literature, based on their involvement in the osteogenesis process or in the bone response to mechanical stimuli. qPCR analysis shows an increase in expression of the chosen targets, and confirms the effectiveness of the presented approach for studying living single cells response to dynamic compression.


Asunto(s)
Estrés Mecánico , Transcriptoma , Actinas/genética , Actinas/metabolismo , Línea Celular , Humanos , Mecanotransducción Celular , Proteína Quinasa 1 Activada por Mitógenos/genética , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Proteína Quinasa 3 Activada por Mitógenos/genética , Proteína Quinasa 3 Activada por Mitógenos/metabolismo , Proyectos Piloto , ARN/aislamiento & purificación , ARN/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Análisis de la Célula Individual , Transcriptoma/genética
17.
J Nanosci Nanotechnol ; 6(8): 2382-5, 2006 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-17037844

RESUMEN

In this paper, we report the metallization of a dsDNA template using a novel photography-derived two-step strategy in which dsDNA is first complexed with Ag(I) ions and then irradiated with UV light at 254 nm. The nucleobases act as light harvesters and sensitizers, triggering the photoreduction of the complexed silver ions. This process yields a silver nanoparticles blueprint along the DNA strand. The silver latent image is then developed by depositing metallic nickel through an electroless plating process. This photography-derived procedure generates very homogeneous and evenly distributed strings of silver-core/nickel-shell nanoparticles. Although still discontinuous, we believe that such chains can serve as the base for obtaining continuous metal nanowires. Furthermore, this process can most likely be extended to other plating metals, resulting in a broadly general procedure for metallizing DNA with a variety of different materials. Because of the intrinsic simplicity in using light as the key step, this methodology might be amenable to large-scale development, eventually leading to a very efficient molecular-photolithography process.


Asunto(s)
ADN/química , Electroquímica/métodos , Nanoestructuras/química , Silicatos de Aluminio/química , Biofisica/métodos , Galvanoplastia/métodos , Iones , Microscopía de Fuerza Atómica , Microscopía Electrónica de Rastreo , Nanopartículas/química , Níquel/química , Impresión , Plata/química , Rayos Ultravioleta
18.
Sci Rep ; 6: 37779, 2016 11 24.
Artículo en Inglés | MEDLINE | ID: mdl-27883075

RESUMEN

We have devised a supramolecular edifice involving His-tagged protein A and antibodies to yield surface immobilized, uniformly oriented, IgG-type, antibody layers with Fab fragments exposed off an electrode surface. We demonstrate here that we can affect the conformation of IgGs, likely pushing/pulling electrostatically Fab fragments towards/from the electrode surface. A potential difference between electrode and solution acts on IgGs' charged aminoacids modulating the accessibility of the specific recognition regions of Fab fragments by antigens in solution. Consequently, antibody-antigen affinity is affected by the sign of the applied potential: a positive potential enables an effective capture of antigens; a negative one pulls the fragments towards the electrode, where steric hindrance caused by neighboring molecules largely hampers the capture of antigens. Different experimental techniques (electrochemical quartz crystal microbalance, electrochemical impedance spectroscopy, fluorescence confocal microscopy and electrochemical atomic force spectroscopy) were used to evaluate binding kinetics, surface coverage, effect of the applied electric field on IgGs, and role of charged residues on the phenomenon described. These findings expand the concept of electrical control of biological reactions and can be used to gate electrically specific recognition reactions with impact in biosensors, bioactuators, smart biodevices, nanomedicine, and fundamental studies related to chemical reaction kinetics.


Asunto(s)
Inmunoglobulina G/química , Anticuerpos/química , Antígenos/química , Técnicas Biosensibles/métodos , Electricidad , Electrodos , Fragmentos Fab de Inmunoglobulinas/química , Conformación Molecular , Análisis Espectral/métodos , Proteína Estafilocócica A/química , Propiedades de Superficie
19.
Biophys Chem ; 208: 26-33, 2016 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-26259784

RESUMEN

The ability to sense mechanical stimuli and elaborate a response to them is a fundamental process in all organisms, driving crucial mechanisms ranging from cell volume regulation up to organ development or regeneration. Nevertheless, only in few cases the underlying molecular players are known. In particular, mammals possess a large variety of mechanoreceptors, providing highly specialized functions in sensory cells, but also several housekeeping molecular systems are involved in the complex mechanism of mechanotransduction. Recently, a new class of almost ubiquitous membrane channels has been identified in mammalians, namely piezo1 and piezo2, that is thought to play a crucial role in the mechanobiology of mammals. This review focuses on recent findings on these novel channels, and highlights open biophysical questions that largely remain to be addressed.


Asunto(s)
Canales Iónicos/metabolismo , Animales , Humanos , Canales Iónicos/química , Canales Iónicos/genética
20.
Ultramicroscopy ; 104(3-4): 220-5, 2005 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-15927397

RESUMEN

Nanomachining and beam-assisted Pt deposition by a focused ion beam (FIB) was used to modify AFM probes for improved electric force measurements. Si(3)N(4) cantilevers have been endowed with a nano-electrode at the tip apex to confine the electro-sensitive area at the very tip. This action results in both a marked decrease of the parasitic capacitive effect and in an improved electric force microscopy (EFM) contrast and resolution, with respect to usual, full metal-coated cantilevers. This fabrication approach is suited to the development of innovative electro-sensitive probes, useful in different scanning probe techniques.

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