RESUMEN
BACKGROUND: This study evaluated the effectiveness of treating pseudarthrosis in rats by using bone marrow cell suspensions or cultures of bone marrow mesenchymal stromal cells METHODS: Thirty-eight specific pathogen-free (SPF) animals were randomly assigned to four groups: Group 1, Control, without surgical intervention; Group 2 (Placebo), experimental model of femoral pseudarthrosis treated only with saline solution; Group 3, experimental model of femoral pseudarthrosis treated with heterologous bone marrow cells suspension; Group 4, experimental model of femoral pseudarthrosis treated with cultures of heterologous mesenchymal stromal cells from bone marrow. When pseudarthrosis was confirmed by simple radiological studies, digital radiography and histopathology after a 120-day postoperative period, Groups 2, 3 and 4 were treated as above. At 30, 60 and 90 days after the treatment, all animals were evaluated by simple radiological studies, and at the end of the experiment, the animals were assessed by computed axial tomography and anatomopathological and histomorphometric examinations. RESULTS: Injected cells were detected in the areas affected by pseudarthrosis using scintigraphy within the first 24 hours after their administration. After 60 days, the animals of Group 3 showed callus formation while the animals of Group 4 presented periosteal reaction and had some consolidated areas. In contrast, Group 2 showed a predominance of fibro-osteoid tissue. After 90 days, bone consolidation and remodeling was observed in all animals from Group 3 whereas animals from Group 4 exhibited partial consolidation and those ones from Group 2 persisted with pseudarthrosis. CONCLUSION: The treatment with heterologous bone marrow cells suspension proved to be effective in the treatment of pseudarthrosis whereas cultures of heterologous bone marrow mesenchymal stromal cells did not show the same potential to aid bone healing.
Asunto(s)
Fémur/patología , Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas/citología , Seudoartrosis/terapia , Animales , Fémur/diagnóstico por imagen , Fémur/cirugía , Inyecciones , Claudicación Intermitente , Masculino , Placebos , Seudoartrosis/diagnóstico por imagen , Seudoartrosis/cirugía , Radiografía , Cintigrafía , Ratas , TomografíaRESUMEN
INTRODUCTION: several pancreatectomy techniques in rats have been described and utilized for research concerning the pancreas endocrine and exocrine functions. However, we did not find a description of any kind of laparoscopic pancreatectomy in rats in the consulted databases. The objective of this study is to describe a laparoscopic splenic lobe pancreatectomy in rats. METHODS: ten Wistars rats weighting more then 300 g were operated for standardizing the laparoscopic pancreatosplenectomy technique, aided by previous descriptions of laparoscopic splenectomy and open pancreatectomy in rats. Adjustments have been progressively adopted for technical refinement. RESULTS: In five animals a low-cost rat laparoscopic set was used. In other five animals we used a standard laparoscopic set. Three rats died early due to different causes: transection of the gastroesophageal junction, hemorrhagic shock and inadvertent colonic injury. The postoperative period of the other seven rats was uneventful until the seventh postoperative day. DISCUSSION: laparoscopic distal pancreatectomy and splenectomy in rats is feasible and safe, even with a low-cost set, in which the results were alike the standard laparoscopic set.
Asunto(s)
Laparoscopía , Neoplasias Pancreáticas , Animales , Laparoscopía/efectos adversos , Laparoscopía/métodos , Modelos Teóricos , Pancreatectomía/efectos adversos , Pancreatectomía/métodos , Neoplasias Pancreáticas/cirugía , Ratas , Ratas Wistar , Esplenectomía/efectos adversos , Esplenectomía/métodosRESUMEN
To verify the viability and functionality of cryopreserved thyroid autotransplantation in rats who underwent total thyroidectomy in the treatment of postoperative hypothyroidism. Thirty-two Wistar rats were randomly assigned into groups (G) with eight animals each: control (CG); simulation (SG); hypothyroidism (HTG) and transplanted (TG). At the beginning and in the 13th week of the experiment, serum levels of total T3, free T4, TSH and calcium were determined. In both the first and 14th weeks, scintigraphic examinations, 99m-Tc pertechnetate radioisotope biodistribution and histopathology were performed. In the 14th week, the expression of proliferating cell nuclear antigen (PCNA) and cellular apoptosis (caspase-3) were also evaluated. In the 13th week, the transplanted animals had normal serum levels of total T3 and free T4. TSH levels showed a tendency towards normality. In the 14th week, scintigraphic exams displayed graft isotopic uptake in all animals in the TG group. Histological examinations 13 weeks after transplantation showed the viability and functionality of thyroid follicles. PCNA revealed significant immunoreactivity of the graft (p < 0.001) when the TG was compared to the CG. There was no difference between CG and TG considering the expression of activated caspase-3. The experimental study confirmed the viability and functionality of thyroid autotransplantation implanted in skeletal muscle with evidence of cell proliferation without cellular apoptosis. This surgical strategy was effective in the treatment of postoperative hypothyroidism.
Asunto(s)
Hipotiroidismo/cirugía , Complicaciones Posoperatorias/cirugía , Glándula Tiroides/trasplante , Tiroidectomía/efectos adversos , Animales , Hipotiroidismo/sangre , Hipotiroidismo/etiología , Masculino , Complicaciones Posoperatorias/sangre , Complicaciones Posoperatorias/etiología , Ratas , Ratas Wistar , Tiroxina/sangre , Trasplante Autólogo , Triyodotironina/sangreRESUMEN
PURPOSE: To evaluate a novel and adapted low-cost set model for laparoscopic surgery in rats. METHODS: Nine Wistar rats underwent two different laparoscopic procedures, splenectomy (n=3) and distal pancreatectomy with splenectomy (n = 6), after assembling a low-cost set replacing the conventional one (monitor, micro camera, image processor, light source, laparoscope and insufflator). The new set included an Android Tablet 10.5 ", a 5mm USB Endoscope and semiautomatic sphygmomanometer monitor. RESULTS: The same surgeon performed the laparoscopic procedures. Total surgical time ranged from 36 to 60 minutes with a mean of 45.8 minutes. Three rats died during the distal pancreatic and splenectomy procedure (33.3%), due to respiratory failure (n = 1), uncontrolled abdominal hemorrhage (n=1) and iatrogenic gastric perforation (n = 1). We followed the other six rats (66.6%) for seven days with no further evidence of complications. CONCLUSIONS: The laparoscopic partial pancreatectomy and splenectomy can be performed with the novel low-cost set assembled in the present experimental study. Both specific training and skills development are required to validate more advanced laparoscopic procedures and achieve a desirable outcome.
Asunto(s)
Laparoscopía/educación , Pancreatectomía/educación , Esplenectomía/educación , Animales , Costos y Análisis de Costo , Laparoscopía/economía , Laparoscopía/métodos , Modelos Animales , Pancreatectomía/economía , Pancreatectomía/métodos , Ratas , Ratas Wistar , Esplenectomía/economía , Esplenectomía/métodosRESUMEN
PURPOSE: To evaluate the most frequent surgical techniques of high-risk colorectal anastomoses in rats. METHODS: Wistar rats were enrolled in three different models comprising inflammatory (TNBS enema), vascular (portal vein occlusion) or obstructive (a non-ischemic constricting ring) mechanisms associated with colonic anastomosis that had accomplished after these former lesions. Histological analyses (Hematoxylin and eosin and Picrosirius red) were performed. RESULTS: All anastomoses techniques were associated with risk factors and had complications, mainly anastomotic leakage. In Study 1, the use of a pharmacological agent, trinitrobenzene sulfonic acid (TNBS) mimicked an inflammatory bowel disease such as Crohn's disease with 50% of anastomosis leakage, the higher percentage among all models tested. In Study 2, after portal ischemia followed by reperfusion it was observed a dense neutrophil infiltrate in the midst of necrotic tissue and fibrin at the anastomotic site and 5 days after the anastomosis, no collagen was produced. In Study 3, 5 days after the mechanical obstruction some denuded areas of epithelium with marked oedema of mucosa and submucosa were seen, at the anastomotic site and anastomosis group showed some reduction of collagen density when compared with Control/Sham group. CONCLUSION: All the experimental surgical techniques tested in rats were associated with high-risk colorectal anastomoses and were useful to study colonic anastomotic healing and intestinal leakage.
Asunto(s)
Fuga Anastomótica , Colon/cirugía , Recto/cirugía , Anastomosis Quirúrgica/efectos adversos , Anastomosis Quirúrgica/métodos , Fuga Anastomótica/diagnóstico por imagen , Fuga Anastomótica/patología , Animales , Modelos Animales de Enfermedad , Ratas , Ratas Wistar , Cicatrización de HeridasRESUMEN
PURPOSE:: To evaluate whether undergraduate students feel motivated to develop surgical skills and know their impression about the importance of having a surgical technique discipline in the curriculum of a medical school. METHODS:: A prospective study including three classes in a row, from the 8th period (n = 265) evaluated the knowledge acquired at the Center of Experimental Surgery, Department of Surgery, School of Medicine of Federal University of Rio de Janeiro (UFRJ). The importance of the discipline for medical training as a way of encouragement to arouse and deepen the interest in surgical technique was emphasized. The questions were scored from 1 to 5 (worst to best grade) . RESULTS:: Concerning the importance of the discipline for medical training, 78% and 18% of the students assigned a score 5 and 4, respectively. Regarding the stimulus to improve their surgical technical skills, 40% and 32% attributed the score 5 and 4, respectively. CONCLUSION:: Undergraduate students from the Medical School of UFRJ effectively shared the understanding that the operative technique bases improve the formative process with significant impact, not only in the development of skills, but also to arouse vocations and stimulate new attitudes aimed to knowledge acquirement in the field of Surgery.
Asunto(s)
Educación de Pregrado en Medicina , Cirugía General/educación , Estudiantes de Medicina , Brasil , Femenino , Humanos , Masculino , Estudios ProspectivosRESUMEN
PURPOSE: To evaluate the actual incidence of both microlithiasis and acute cholecystitis during treatment with intravenous ceftriaxone in a new rabbit model. METHODS: New Zealand rabbits were treated with intravenous ceftriaxone or saline for 21 days. Ultrasound monitoring of the gallbladder was performed every seven days until the 21st day when histopathology, immunohistochemistry for proliferating cell nuclear antigen (PCNA), pro-caspase-3 and CD68, liver enzyme biochemistry, and chromatography analysis of the bile and sediments were also performed. RESULTS: All animals treated with ceftriaxone developed acute cholecystitis, confirmed by histopathology (P<0.05) and biliary microlithiasis, except one that exhibited sediment precipitation. In the group treated with ceftriaxone there was an increase in pro-caspase-3, gamma-glutamyl transpeptidase concentration, PCNA expression and in the number of cells positive for anti-CD68 (P<0.05). In the ceftriaxone group, the cholesterol and lecithin concentrations increased in the bile and a high concentration of ceftriaxone was found in the microlithiasis. CONCLUSION: Ceftriaxone administered intravenously at therapeutic doses causes a high predisposition for lithogenic bile formation and the development of acute lithiasic cholecystitis.
Asunto(s)
Antibacterianos/efectos adversos , Ceftriaxona/efectos adversos , Colecistectomía , Colecistitis Aguda/inducido químicamente , Colelitiasis/inducido químicamente , Administración Intravenosa , Animales , Antibacterianos/administración & dosificación , Ceftriaxona/administración & dosificación , Colecistectomía Laparoscópica , Colecistitis Aguda/metabolismo , Colelitiasis/metabolismo , Modelos Animales de Enfermedad , Vesícula Biliar/patología , Conejos , Investigación Biomédica TraslacionalRESUMEN
The authors detail the experimental development of a technique for the reconstruction of the ureter using a tubular shape, muscle flap of the abdominal wall. the preliminary results indicate the feasibility of this surgical technique.
Asunto(s)
Colgajos Quirúrgicos , Uréter/cirugía , Pared Abdominal/cirugía , Animales , Masculino , Conejos , Procedimientos Quirúrgicos Urológicos/métodosRESUMEN
OBJECTIVE: To gather and clarify the actual effects of low-level laser therapy on wound healing and its most effective ways of application in human and veterinary medicine. METHODS: We searched original articles published in journals between the years 2000 and 2011, in Spanish, English, French and Portuguese languages, belonging to the following databases: Lilacs, Medline, PubMed and Bireme; Tey should contain the methodological description of the experimental design and parameters used. RESULTS: doses ranging from 3 to 6 J/cm(2) appear to be more effective and doses 10 above J/cm(2) are associated with deleterious effects. The wavelengths ranging from 632.8 to 1000 nm remain as those that provide more satisfactory results in the wound healing process. CONCLUSION: Low-level laser can be safely applied to accelerate the resolution of cutaneous wounds, although this fact is closely related to the election of parameters such as dose, time of exposure and wavelength.
Asunto(s)
Terapia por Luz de Baja Intensidad , Cicatrización de Heridas/efectos de la radiación , Animales , HumanosRESUMEN
Abstract Purpose: To evaluate a novel and adapted low-cost set model for laparoscopic surgery in rats. Methods: Nine Wistar rats underwent two different laparoscopic procedures, splenectomy (n=3) and distal pancreatectomy with splenectomy (n = 6), after assembling a low-cost set replacing the conventional one (monitor, micro camera, image processor, light source, laparoscope and insufflator). The new set included an Android Tablet 10.5 ", a 5mm USB Endoscope and semiautomatic sphygmomanometer monitor. Results: The same surgeon performed the laparoscopic procedures. Total surgical time ranged from 36 to 60 minutes with a mean of 45.8 minutes. Three rats died during the distal pancreatic and splenectomy procedure (33.3%), due to respiratory failure (n = 1), uncontrolled abdominal hemorrhage (n=1) and iatrogenic gastric perforation (n = 1). We followed the other six rats (66.6%) for seven days with no further evidence of complications. Conclusions: The laparoscopic partial pancreatectomy and splenectomy can be performed with the novel low-cost set assembled in the present experimental study. Both specific training and skills development are required to validate more advanced laparoscopic procedures and achieve a desirable outcome.
Asunto(s)
Animales , Ratas , Pancreatectomía/educación , Esplenectomía/educación , Laparoscopía/educación , Pancreatectomía/economía , Pancreatectomía/métodos , Esplenectomía/economía , Esplenectomía/métodos , Ratas Wistar , Laparoscopía/economía , Laparoscopía/métodos , Costos y Análisis de Costo , Modelos AnimalesRESUMEN
Abstract Purpose: To evaluate the most frequent surgical techniques of high-risk colorectal anastomoses in rats. Methods: Wistar rats were enrolled in three different models comprising inflammatory (TNBS enema), vascular (portal vein occlusion) or obstructive (a non-ischemic constricting ring) mechanisms associated with colonic anastomosis that had accomplished after these former lesions. Histological analyses (Hematoxylin and eosin and Picrosirius red) were performed. Results: All anastomoses techniques were associated with risk factors and had complications, mainly anastomotic leakage. In Study 1, the use of a pharmacological agent, trinitrobenzene sulfonic acid (TNBS) mimicked an inflammatory bowel disease such as Crohn's disease with 50% of anastomosis leakage, the higher percentage among all models tested. In Study 2, after portal ischemia followed by reperfusion it was observed a dense neutrophil infiltrate in the midst of necrotic tissue and fibrin at the anastomotic site and 5 days after the anastomosis, no collagen was produced. In Study 3, 5 days after the mechanical obstruction some denuded areas of epithelium with marked oedema of mucosa and submucosa were seen, at the anastomotic site and anastomosis group showed some reduction of collagen density when compared with Control/Sham group. Conclusion: All the experimental surgical techniques tested in rats were associated with high-risk colorectal anastomoses and were useful to study colonic anastomotic healing and intestinal leakage.
Asunto(s)
Animales , Ratas , Recto/cirugía , Colon/cirugía , Fuga Anastomótica/patología , Fuga Anastomótica/diagnóstico por imagen , Cicatrización de Heridas , Anastomosis Quirúrgica/efectos adversos , Anastomosis Quirúrgica/métodos , Ratas Wistar , Modelos Animales de EnfermedadRESUMEN
Abstract Purpose: To evaluate whether undergraduate students feel motivated to develop surgical skills and know their impression about the importance of having a surgical technique discipline in the curriculum of a medical school. Methods: A prospective study including three classes in a row, from the 8th period (n = 265) evaluated the knowledge acquired at the Center of Experimental Surgery, Department of Surgery, School of Medicine of Federal University of Rio de Janeiro (UFRJ). The importance of the discipline for medical training as a way of encouragement to arouse and deepen the interest in surgical technique was emphasized. The questions were scored from 1 to 5 (worst to best grade) . Results: Concerning the importance of the discipline for medical training, 78% and 18% of the students assigned a score 5 and 4, respectively. Regarding the stimulus to improve their surgical technical skills, 40% and 32% attributed the score 5 and 4, respectively. Conclusion: Undergraduate students from the Medical School of UFRJ effectively shared the understanding that the operative technique bases improve the formative process with significant impact, not only in the development of skills, but also to arouse vocations and stimulate new attitudes aimed to knowledge acquirement in the field of Surgery.
Asunto(s)
Humanos , Masculino , Femenino , Estudiantes de Medicina , Cirugía General/educación , Educación de Pregrado en Medicina , Brasil , Estudios ProspectivosRESUMEN
Abstract Purpose: To evaluate the actual incidence of both microlithiasis and acute cholecystitis during treatment with intravenous ceftriaxone in a new rabbit model. Methods: New Zealand rabbits were treated with intravenous ceftriaxone or saline for 21 days. Ultrasound monitoring of the gallbladder was performed every seven days until the 21st day when histopathology, immunohistochemistry for proliferating cell nuclear antigen (PCNA), pro-caspase-3 and CD68, liver enzyme biochemistry, and chromatography analysis of the bile and sediments were also performed. Results: All animals treated with ceftriaxone developed acute cholecystitis, confirmed by histopathology (P<0.05) and biliary microlithiasis, except one that exhibited sediment precipitation. In the group treated with ceftriaxone there was an increase in pro-caspase-3, gamma-glutamyl transpeptidase concentration, PCNA expression and in the number of cells positive for anti-CD68 (P<0.05). In the ceftriaxone group, the cholesterol and lecithin concentrations increased in the bile and a high concentration of ceftriaxone was found in the microlithiasis. Conclusion: Ceftriaxone administered intravenously at therapeutic doses causes a high predisposition for lithogenic bile formation and the development of acute lithiasic cholecystitis.
Asunto(s)
Animales , Ratas , Ceftriaxona/efectos adversos , Colecistectomía , Colelitiasis/inducido químicamente , Colecistitis Aguda/inducido químicamente , Antibacterianos/efectos adversos , Ceftriaxona/administración & dosificación , Colelitiasis/metabolismo , Colecistectomía Laparoscópica , Colecistitis Aguda/metabolismo , Modelos Animales de Enfermedad , Investigación Biomédica Traslacional , Administración Intravenosa , Vesícula Biliar/patología , Antibacterianos/administración & dosificaciónRESUMEN
PURPOSE: The aim of this study was to describe an experimental surgical technique to be used in the evaluation of bone grafts in rabbits. METHODS: The operative technique was performed in jaws, with external approach from the labial commissure to the last molar tooth. An incision about 1cm extended forward to the alveolar ridge, followed by a exposing the cervical region of the alveolar bone and dental elements was done. Thus, after extraction of first molar with forceps, the tooth socket left was filled with hydroxyapatite. The bone gain was analyzed by histopathological studies. RESULTS: The histological analyses indicated formed bone surrounding the biomaterial. CONCLUSION: The experimental model using the tooth socket of the rabbit molar is a feasible procedure for studies of bone grafts.
Asunto(s)
Materiales Biocompatibles/administración & dosificación , Durapatita/administración & dosificación , Diente Molar/cirugía , Osteogénesis/fisiología , Extracción Dental , Alveolo Dental/cirugía , Animales , Trasplante Óseo/métodos , Masculino , Modelos Animales , Conejos , Reproducibilidad de los Resultados , Alveolo Dental/anatomía & histologíaRESUMEN
PURPOSE: To propose a novel model of pseudarthrosis in a small animal and to investigate the ability of backscatter parameters from ultrasound signals in differentiating normal bone from those ones with pseudarthrosis. METHODS: Twelve Rattus norvegicus albinus free from pathogenic species (SPF) were randomly divided in two groups, with six animals each. In the Control group a surgical approach to the femur was made, followed by the synthesis of the muscle and skin layers. The Experimental group was submitted to an osteotomy of the femur and a vascularized flap of the fascia lata was interposed in the line of the fractured bone. Then the alignment and bone stabilization were accomplished, by using nylon stitch in U shape introduced in holes made in the proximal and distal fractured bone. Bone samples were scanned with ultrasound and signals were collected for each one to analyze the parameter Apparent Integrated Backscatter - AIB. RESULTS: Radiological and anatomopathologic studies revealed the absence of bone consolidation with persistence of fiber-osteoid tissue. Values of the ultrasound parameter AIB from normal bones were statistically different from those with pseudarthrosis. CONCLUSION: The experimental model was suitable for pseudarthrosis development in rats and the ultrasound backscatter parameters were able to identify such a bone disease in vitro.
Asunto(s)
Seudoartrosis/diagnóstico por imagen , Animales , Modelos Animales de Enfermedad , Fémur/diagnóstico por imagen , Fémur/cirugía , Distribución Aleatoria , Ratas , Estadísticas no Paramétricas , UltrasonografíaRESUMEN
OBJECTIVE: To gather and clarify the actual effects of low-level laser therapy on wound healing and its most effective ways of application in human and veterinary medicine. METHODS: We searched original articles published in journals between the years 2000 and 2011, in Spanish, English, French and Portuguese languages, belonging to the following databases: Lilacs, Medline, PubMed and Bireme; Tey should contain the methodological description of the experimental design and parameters used. RESULTS: doses ranging from 3 to 6 J/cm2 appear to be more effective and doses 10 above J/cm2 are associated with deleterious effects. The wavelengths ranging from 632.8 to 1000 nm remain as those that provide more satisfactory results in the wound healing process. CONCLUSION: Low-level laser can be safely applied to accelerate the resolution of cutaneous wounds, although this fact is closely related to the election of parameters such as dose, time of exposure and wavelength. .
OBJETIVO: reunir e esclarecer quais os reais efeitos da laserterapia de baixa potência sobre feridas cutâneas e suas formas mais eficazes de aplicação na medicina humana e veterinária. MÉTODOS: foram pesquisados Artigos Originais publicados em periódicos pertencentes às seguintes bases de dados: Lilacs, MedLine, Bireme e PubMed entre os anos de 2000 e 2011, na línguas espanhola, inglesa, francesa e portuguesa, que contivessem a descrição metodológica do modelo experimental e parâmetros utilizados no estudo. RESULTADOS: doses compreendidas entre 3-6 J/cm2 parecem ser mais eficazes e que doses acima de 10 J/cm2 estão associadas a efeitos deletérios. Os comprimentos de onda compreendidos entre 632,8-1000nm seguem como aqueles que apresentam resultados mais satisfatórios no processo de cicatrização tecidual. CONCLUSÃO: o laser de baixa potência pode ser indicado com segurança para acelerar a resolução de feridas cutâneas, muito embora este fato esteja intimamente ligado à eleição de parâmetros como dose, tempo e comprimento de onda. .
Asunto(s)
Animales , Humanos , Terapia por Luz de Baja Intensidad , Cicatrización de Heridas/efectos de la radiaciónRESUMEN
OBJECTIVES: To establish a model of chronic renal failure in rabbits, with perspectives of its use for therapeutic and repairing actions. METHODS: Nineteen males, adults rabbits (New Zealand) randomly distributed into three groups were used: Group 1 - Control (n =5); Group 2-Sham (n =7); and Group 3 - Experimental (n =7). They were anaesthetized by using intramuscular Cetamine, Diazepam and Fentanyl followed by Sevorane with vaporizer device. In Group 3, a bipolar left nephrectomy was carried out and after four weeks, it was also done a right nephrectomy. All the samples of the renal tissue were weighed. The Group 2 was only submitted to both abdominal laparotomies, without nephrectomy. Biochemical evaluations, with urea, creatinina, sodium and potassium measurement; abdominal ultrasound scan; scintigraphy and histological analysis were performed in all animals. RESULTS: In group 3 there was a progressive increase of urea (p=0.0001), creatinine (p=0.0001), sodium (p = 0,0002) and potassium (p=0,0003). The comparison of these results with those one of the Groups 1 and 2, in all intervals, revealed blood rising with statistical significant level (p < 0,05). In Group 3, the ultrasound scan identified an increasing of the left kidney size, after 16 weeks and at the 4th week the scintigraphy confirmed the loss of 75% of the left renal mass. In the same group, the histological evaluation showed subcapsular and intersticial fibrosis and also tubular regeneration. CONCLUSION: The experimental model of IRC is feasible, with animal's survival in middle term which allows the use of this interval like a therapeutic window for testing different approaches in order to repair the kidney damages.
Asunto(s)
Modelos Animales de Enfermedad , Fallo Renal Crónico/cirugía , Animales , Masculino , ConejosRESUMEN
OBJECTIVE: We sought to describe a new model of pseudoarthrosis in rats free of pathogenic species (Rattus norvegicus albinus - SPF). METHODS: The animals were randomly distributed into two groups were used: Group 1 - Control (n =6); Group 2 - Experimental (n =6). In the Control group an access to the femur followed by the synthesis of the muscular and skin layers was done. The Experimental group was submitted to osteotomy of the femur and a pedicle flap of the fascia lata was interposed between the segments resulting from the surgically induced fracture. The procedure was carried on with both alignment and approximation of the fractured bone through the use of mononylon thread introduced into holes created in the proximal and distal. RESULTS: Radiological and anatomopathological studies showed the absence of bone consolidation with persistence of fibro-osteoid tissue up to 120 days of follow-up. CONCLUSION: The experimental model is suitable for the development of a pseudoarthrosis in rats.
Asunto(s)
Modelos Animales de Enfermedad , Fracturas no Consolidadas/complicaciones , Osteotomía/métodos , Seudoartrosis/etiología , Animales , Femenino , Masculino , Seudoartrosis/diagnóstico por imagen , Seudoartrosis/patología , Radiografía , Distribución Aleatoria , RatasRESUMEN
OBJECTIVE: To evaluate extra-capsular technique for the treatment of the anterior cruciate ligament rupture, in dogs. METHODS: It was performed an extra-articulate surgery without arthrotomy of the anterior cruciate ligament by using a strip of the autogenous fascia lata for stabilization of the knee joint in six animals which had severe claudication and drawer movement sign positive. RESULTS: The extra articulate technique was effective with good knee stabilization and satisfactory outcome. CONCLUSION: The use of the fascia lata with an extra capsular technique to fix the rupture of the anterior cruciate ligament seems to be useful once it is a simple and rapid procedure with minimum tissue damage and efficient postoperative recovery.
Asunto(s)
Lesiones del Ligamento Cruzado Anterior , Ligamento Cruzado Anterior/cirugía , Fascia Lata/trasplante , Animales , Modelos Animales de Enfermedad , Perros , Modelos Animales , Procedimientos OrtopédicosRESUMEN
PURPOSE: The aim of this study was to describe an experimental surgical technique to be used in the evaluation of bone grafts in rabbits. METHODS: The operative technique was performed in jaws, with external approach from the labial commissure to the last molar tooth. An incision about 1cm extended forward to the alveolar ridge, followed by a exposing the cervical region of the alveolar bone and dental elements was done. Thus, after extraction of first molar with forceps, the tooth socket left was filled with hydroxyapatite. The bone gain was analyzed by histopathological studies. RESULTS: The histological analyses indicated formed bone surrounding the biomaterial. CONCLUSION: The experimental model using the tooth socket of the rabbit molar is a feasible procedure for studies of bone grafts.
OBJETIVO: Descrever uma técnica cirúrgica em coelhos para avaliação de enxertos ósseos. MÉTODOS: A técnica operatória consiste numa incisão na topografia da comissura labial até o ultimo molar. Uma incisão de 1cm no rebordo para expor o osso alveolar e a região cervical dos molares. Realizou-se a exodontia com fórceps e o alvéolo é preenchido com hidroxiapatita. Após o tempo de cicatrização é realizada a avaliação histológica. RESULTADOS: A análise histológica mostrou o novo osso formado rodeando biomaterial. CONCLUSÃO: O modelo cirúrgico para avaliação de enxertos ósseos utilizando molares de coelhos se mostrou factível.