Aberrant P-cadherin expression is associated to aggressive feline mammary carcinomas.

BACKGROUND: Cadherins are calcium-dependent cell-to-cell adhesion glycoproteins playing a critical role in the formation and maintenance of normal tissue architecture. In normal mammary gland, E-cadherin is expressed by luminal epithelial cells, while P-cadherin is restricted to myoepithelial cells. Changes in the expression of classical E- and P-cadherins have been observed in mammary lesions and related to mammary carcinogenesis. P-cadherin and E-cadherin expressions were studied in a series of feline normal mammary glands, hyperplastic/dysplastic lesions, benign and malignant tumours by immunohistochemistry and double-label immunofluorescence. RESULTS: In normal tissue and in the majority of hyperplastic/dysplastic lesions and benign tumours, P-cadherin was restricted to myoepithelial cells, while 80% of the malignant tumours expressed P-cadherin in luminal epithelial cells. P-cadherin expression was significantly related to high histological grade of carcinomas (p <0.0001), tumour necrosis (p = 0.001), infiltrative growth (p = 0.0051), and presence of neoplastic emboli (p = 0.0401). Moreover, P-cadherin positive carcinomas had an eightfold likelihood of developing neoplastic emboli than negative tumours. Cadherins expression profile in high grade and in infiltrative tumours was similar, the majority expressing P-cadherin, regardless of E-cadherin expression status. The two cadherins were found to be co-expressed in carcinomas with aberrant P-cadherin expression and preserved E-cadherin. CONCLUSIONS: The results demonstrate a relationship between P-cadherin expression and aggressive biological behaviour of feline mammary carcinomas, suggesting that P-cadherin may be considered an indicator of poor prognosis in this animal species. Moreover, it indicates that, in queens, the aberrant expression of P-cadherin is a better marker of mammary carcinomas aggressive behaviour than the reduction of E-cadherin expression. Further investigation with follow-up studies in feline species should be conducted in order to evaluate the prognostic value of P-cadherin expression in E-cadherin positive carcinomas.

Distribution of Inflammatory Infiltrate in Feline Mammary Lesions: Relationship With Clinicopathological Features.

Inflammation is a frequent finding in feline mammary neoplasms. Recent research suggests that the presence and location of tumour-associated immune cells might play a significant role in the clinical outcome of feline mammary carcinomas. The present study aimed to characterise the overall inflammatory infiltrates in healthy, hyperplastic/dysplastic, benign and malignant lesions of the feline mammary gland, and to evaluate its association with clinicopathological features. Perilesional and intralesional inflammatory foci were evaluated in 307 lesions from 185 queens, and categorised according to its distribution and intensity. The presence, location and density of tertiary lymphoid structures were also assessed. A control group included 24 queens without mammary changes. The presence of intralesional and perilesional inflammatory infiltrate was observed in a majority of the lesions (80.8% and 90.2%, respectively), but differed according to the type of mammary lesion, being more remarkable in malignant neoplasms. Only scarce individual cells were observed in 28.1% of the normal mammary glands. Data analysis revealed statistically significant associations (p < 0.05) between the presence of a more prominent intralesional and perilesional inflammatory reaction and several clinicopathological features associated with worse prognosis, including clinical stage, tumour size, mitotic count, lymphovascular invasion and lymph node metastasis. Furthermore, tertiary lymphoid structures were significantly more frequent in tumours with an infiltrative growth and lymph node metastasis. According to our results, the inflammatory reaction present in different types of feline mammary lesions is associated with the development of more aggressive tumours.

Eyelid Reconstruction and Infraorbital Repair Using a Mustardé Flap Technique in a Dog.

This case report describes a 5-year-old dog with a defect in the right eyelid, absence of orbicularis musculature, and absence of cutaneous tissue in the infraorbital region, submitted to the Mustardé flap technique. A large rotational flap was performed, including a cartilage graft from the outer ear, to correct the defect in the lower eyelid and infraorbital region. Also, euryblepharon correction of the upper eyelid was performed with wedge excision. The techniques were performed in a single surgical step and with appropriated functional and aesthetic results. The described approach may be employed as a surgical option in large eyelid defects.

Characterization of α-, ß- and p120-Catenin Expression in Feline Mammary Tissues and their Relation with E- and P-Cadherin.

BACKGROUND: Abnormal catenin expression has been related to mammary carcinogenesis in both human and canine species and they are considered tumor- and invasion-suppressor molecules; however, in feline mammary tissues they have been scarcely studied. MATERIALS AND METHODS: The immunohistochemical expression of α-, ß- and p120-catenin was studied in a series of normal feline mammary glands, hyperplastic/dysplastic lesions and benign and malignant mammary tumors. Their relationship with clinicopathological parameters and with E- and P-cadherin expression was assessed. RESULTS: Normal tissues, hyperplastic/dysplastic lesions and benign tumors expressed α-, ß- and p120-catenin in the membrane of more than 75% of the luminal epithelial cells, while in malignant tumors, there was a reduction in their membranous expression and a p120-catenin cytoplasmic expression in 40%. Reduced α-catenin expression was related to tumor features with prognostic value, namely tumor size (p=0.0203) and necrosis (p=0.0205). The expression of α-, ß- and p120-catenin were individually related to each other and collectively associated with E-cadherin expression. CONCLUSION: The results demonstrate a relationship between feline mammary carcinogenesis and decreased expression of catenins, suggesting that they may represent a valuable tool in the diagnosis of feline mammary neoplasms.

P-cadherin expression in feline mammary tissues.

The search for molecular markers in the feline mammary gland, namely, the adhesion molecules belonging to the cadherin family, is useful in the understanding of the development of mammary carcinomas in felines and humans. To study P-cadherin expression in the feline mammary gland, 61 samples of normal (n = 4), hyperplastic (n = 12), and neoplastic (n = 45) feline mammary tissues were examined. In both normal and hyperplastic mammary tissues as well as in benign tumours, P-cadherin immunolabelling was restricted to myoepithelial cells. In malignant tumours, however, there was an aberrant epithelial P-cadherin immunoexpression in 64.1% (n = 25) of cases, with a membranous and/or cytoplasmic pattern of distribution. A statistically significant relationship was seen between epithelial P-cadherin expression and malignant mammary lesions (P = 0.0001). In malignant mammary tumours, there was likewise a statistically significant relationship between aberrant P-cadherin immunoexpression and histological grade (P = 0.0132). Aberrant epithelial P-cadherin expression seems to be related to malignancy in the feline mammary gland. To confirm the results of this investigation, further studies with larger samples and follow-up studies are warranted.

Relatório de estágio de intervenção comunitária: hábitos de vida saudáveis dos adolescentes da Escola Secundária Mouzinho da Silveira de Portalegre / Community intervention internship report: healthy lifestyle habits of adolescents at the Mouzinho da Silveira Secondary School in Portalegre

A adolescência deve ser considerada como uma etapa do ciclo vital do ser humano, onde ocorrem importantes processos de crescimento e desenvolvimento. É um período de transição da infância para a idade adulta, sendo difícil estabelecer uma delimitação etária do princípio e do seu fim, uma vez que existem «muitas adolescências¼ conforme cada infância, cada fase de maturação, cada família, cada época, cada cultura. Enquanto se tenta entender a si próprio e aos outros, o adolescente experiencia novas sensações, diferentes sentimentos, passando por situações únicas na sua vida. Coabitam nesta fase desejos ambivalentes de crescimento e regressão, de autonomia e dependência, ligado ao passado, vontade de se projetar no futuro. É durante a adolescência que se poderão verificar comportamentos e o estabelecer de hábitos de vida prejudiciais à sua saúde, com implicações no momento e/ou a longo prazo. Políticas de Promoção da Saúde envolvem a implementação estratégica de Programas de Educação para a Saúde, com vista a capacitar os adolescentes para a aquisição e/ou manutenção de hábitos de vida saudáveis, possuindo deste modo um papel fundamental e decisivo na sua saúde. No âmbito do III Mestrado em Enfermagem, com a área de Especialização em Enfermagem Comunitária, o presente relatório ambiciona descrever, analisar e refletir as atividades desenvolvidas durante o estágio. O estágio sucedeu o Diagnóstico da Situação de Saúde, efetuado em grupo (4 mestrandas), tendo decorrido na Escola Secundaria Mouzinho da Silveira em Portalegre, junto dos adolescentes do 8.º e 9.º Anos, no Ano Letivo 2013/2014, durante o período de 16 de setembro de 2013 a 31 de janeiro de 2014. Elegeu-se a comunidade adolescente, dado o elevado interesse desta para a saúde comunitária, com o intuito de se promover a capacitação dos adolescentes na aquisição/manutenção de Hábitos de Vida Saudáveis. Com a efetivação do estágio, tornou-se um desafio para a mestranda a aquisição de competências como enfermeira Especialista em Enfermagem Comunitária.

The adolescence should be considered as a stage of the human being's life cycle where there are major growth and development processes. It is a transition period from childhood to adulthood, being difficult to establish bounds for its beginning and its end, since there are «many adolescences¼ depending on each childhood, maturation phase, family, era, culture. While trying to understand oneself and the others, the adolescent experiences new sensations, different feelings, going through unique situations in his life. In this phase there co-exist ambivalent desires of growth and regression, autonomy and dependence, attached to the past, willing to project himself into the future. It is during the adolescence that behaviours and the establishment of harmful living habits to one's health can be verified, with immediate implications and/or at long term. Health Promotion policies involve the strategic implementation of Education Programs for Health, aiming to enable adolescents for the acquisition and/or maintenance of healthy living habits, thus having an essential and decisive role in their health. Within the III Master's Degree in Nursing, with the area of specialisation in Community Nursing, this report aims to describe, analyse and reflect the activities developed during the internship. The internship succeeded the Health Situation Diagnosis, made in group (4 master's students), at the Secondary School Mouzinho da Silveira in Portalegre, among adolescents of the 8th and 9th grades, in the academic year 2013/2014, from 16th september 2013 to 31st january 2014. The adolescent community was elected given its high interest for the community health, in order to promote the adolescents capacity in the acquisition/maintenance of Healthy Living Habits. With the conclusion of the internship, the acquisition of skills as a Community Nursing Specialist nurse has become a challenge for the master's student.