Correlation of Ductoscopic and Histopathological Findings and Their Relevance as Predictors for Malignancy: A German Multicenter Study.

BACKGROUND/AIM: The study aimed at investigating the correlation between ductoscopic and histopathological findings and clarify whether the former allow for accurate prediction of malignancy. PATIENTS AND METHODS: The prospective national multi-center study covered a sample of 224 patients with pathologic nipple discharge. A total of 214 patients underwent ductoscopy with subsequent extirpation of the mammary duct. The ductoscopic findings were categorized according to shape, number, color and surface structure of lesions and vascularity and compared to the histological results and analyses. RESULTS: Ductoscopy revealed lesions in 134 of 214 patients (62.2%). The criteria "multiple versus solitary lesion" differed significantly between malignant and benign lesions. All other criteria were not statistically significant. Malignant tumors were more frequently presented as multiple lesions, benign lesions or masses as solitary lesions (80% vs. 24.8%; p=0.018). CONCLUSION: The ductoscopic criterion "solitary vs. multiple lesion" appears to have a low diagnostic prediction of malignancy or benignity.

Cold Physical Plasma Selectively Elicits Apoptosis in Murine Pancreatic Cancer Cells In Vitro and In Ovo.

BACKGROUND/AIM: Poor prognosis of pancreatic cancer has remained almost unchanged in recent years. Cold physical plasma was suggested as an innovative anticancer strategy, but its selective killing activity of malignant over non-malignant cells has only partially been explored. The present study aimed at exploring the effect of cold physical plasma on cellular viability. MATERIALS AND METHODS: Induction of cell death and apoptosis by cold physical plasma was investigated in murine PDA6606 pancreatic cancer cells and primary murine fibroblasts in vitro (2D and 3D cultures) and in ovo. RESULTS: Plasma increased apoptosis in PDA6606 to a significantly higher extent compared to fibroblasts. Antioxidants abrogated these effects, suggesting a prime role of reactive oxygen species in plasma-induced apoptosis. Plasma increased apoptosis of 3D PDA6606 multicellular spheres grown in vitro and in ovo, to significantly higher rates compared to that of fibroblasts, with minimum in ovo inflammation or necrosis observed by hematoxylin and eosin staining (H&E). CONCLUSION: These data support the future intra-operative application of cold physical plasma for the treatment of microscopic residual tumor tissue after surgical resection.

Diagnosis and treatment of cardiac echinococcosis.

Cardiac echinococcosis is a rare manifestation of cystic echinococcosis (CE) caused by the tapeworm Echinococcus granulosus Among all patients suffering from CE, only 0.5%-2% exhibit a cardiac involvement. In addition, during the past years the number of CE cases reported in Western Europe remained roughly unchanged. However, we postulate that cases of CE in Western Europe will increase due to a growing number of refugees coming from endemic areas such as Southern Europe, Eastern Europe and the Middle East. Importantly, although cardiac echinococcosis is rare the disease can lead to many clinical complications, for instance acute heart failure and life-threatening arrhythmias. With respect to the increasing relevance of cardiac echinococcosis in Western Europe and the danger of fulminant disease courses, here we review diagnosis strategies and treatment options of the disease. Diagnosis of cardiac echinococcosis requires a detailed evaluation of the patients' case history, specific laboratory analyses and radiological imaging methods. Ultrasound, MRI and CT are key imaging tools for diagnosis, therapy control, prognosis estimation and disease course control. For the therapy of cardiac echinococcosis, a combination of surgical removal and drug treatment should be applied to symptomatic as well as asymptomatic patients. The complete surgical removal of the cyst(s) is the major prognosis factor of the cardiac manifestation of CE.

Pericardial Tamponade in an Adult Suffering from Acute Mumps Infection.

Here, we report a case of a 51-year-old man with acute pericardial tamponade requiring emergency pericardiocentesis after he suffered from sore throat, headache, malaise, and sweats for two weeks. Serological analyses revealed increased mumps IgM and IgG indicating an acute mumps infection whereas other bacterial and viral infections were excluded. In addition, MRI revealed atypical swelling of the left submandibular gland. Whereas mumps has become a rare entity in children due to comprehensive vaccination regimens in western civilizations, our case highlights mumps as an important differential diagnosis also in adults, where the virus can induce life-threatening complications such as pericardial tamponade.

Use of the reverse latissimus muscle flap for closure of complex back wounds in patients with spinal cord injury.

STUDY DESIGN: Our study was designed as a retrospective review of spinal cord patients with complex back wounds in whom the reverse latissimus muscle flap was used for closure. OBJECTIVES: The objective was to evaluate the efficacy and outcomes in these patients. SUMMARY OF BACKGROUND DATA: Earlier publications give only anecdotal reports on the treatment of complex back wounds in patients with spinal cord syndrome. The data so far available do not allow any comparison of the various methods used. METHODS: Fourteen patients with congenital or acquired spinal cord syndrome and with an average age of 31.2 years (range 12-76 years) had complex back wounds in the thoracic and lumbar regions, which were closed in each case with a reverse latissimus dorsi muscle flap. The patients had deep wound infection and wound dehiscence following spondylodesis (n = 6), dead space and wound dehiscence following laminectomy (n = 6), or decubitus ulcers over the spine (n = 2). The spinal cord syndrome was chronic in nine patients and acute in five. The pre- and postoperative treatment and the actual operation were all done in a spinal cord injury center. In nine patients, the back wound was closed in a primary procedure, and in five, a split-thickness skin graft was used to close it. In 11 patients, primary wound healing was achieved with no complications after closure, whereas in 3 patients, complications with the back wound or the donor site led to disturbances of wound healing lasting up to 25 weeks. Five operations had to be performed specifically because of these complications. RESULTS: At follow-up 27.4 months (mean; range 8-114 months) after the operation, all back wounds were closed, free of irritation, and completely healed. The patients did not report any handicaps in everyday life, loss of strength in the upper extremities, or functional restrictions. One patient had a deep vein thrombosis in one leg. No further complications linked with the spinal cord syndrome were ascertained. The instrumentation used during the spondylodesis operation was left in place in four cases. The spondylodesis became solidly fused in each of these cases. CONCLUSION: Use of the reverse latissimus muscle flap is a reliable method of closing complex back wounds in patients with spinal cord syndrome. Should there be complications in the area of the flap, secondary wound healing can be achieved by wound debridement and closure of the wound by means of split-thickness skin grafting. There is no loss of function in the upper extremities. The complications typically seen in spinal cord patients can be avoided when the treatment is carried out in the conditions that obtain in a specialized spinal cord injury center.