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1.
Molecules ; 24(7)2019 Apr 03.
Artículo en Inglés | MEDLINE | ID: mdl-30987110

RESUMEN

The hippocampus is thought to encode information by altering synaptic strength via synaptic plasticity. Some forms of synaptic plasticity are induced by lipid-based endocannabinoid signaling molecules that act on cannabinoid receptors (CB1). Endocannabinoids modulate synaptic plasticity of hippocampal pyramidal cells and stratum radiatum interneurons; however, the role of endocannabinoids in mediating synaptic plasticity of stratum oriens interneurons is unclear. These feedback inhibitory interneurons exhibit presynaptic long-term potentiation (LTP), but the exact mechanism is not entirely understood. We examined whether oriens interneurons produce endocannabinoids, and whether endocannabinoids are involved in presynaptic LTP. Using patch-clamp electrodes to extract single cells, we analyzed the expression of endocannabinoid biosynthetic enzyme mRNA by reverse transcription and then real-time PCR (RT-PCR). The cellular expression of calcium-binding proteins and neuropeptides were used to identify interneuron subtype. RT-PCR results demonstrate that stratum oriens interneurons express mRNA for both endocannabinoid biosynthetic enzymes and the type I metabotropic glutamate receptors (mGluRs), necessary for endocannabinoid production. Immunohistochemical staining further confirmed the presence of diacylglycerol lipase alpha, an endocannabinoid-synthesizing enzyme, in oriens interneurons. To test the role of endocannabinoids in synaptic plasticity, we performed whole-cell experiments using high-frequency stimulation to induce long-term potentiation in somatostatin-positive cells. This plasticity was blocked by AM-251, demonstrating CB1-dependence. In addition, in the presence of a fatty acid amide hydrolase inhibitor (URB597; 1 µM) and MAG lipase inhibitor (JZL184; 1 µM) that increase endogenous anandamide and 2-arachidonyl glycerol, respectively, excitatory current responses were potentiated. URB597-induced potentiation was blocked by CB1 antagonist AM-251 (2 µM). Collectively, this suggests somatostatin-positive oriens interneuron LTP is CB1-dependent.


Asunto(s)
Endocannabinoides/biosíntesis , Hipocampo/fisiología , Potenciación a Largo Plazo , Receptor Cannabinoide CB1/genética , Receptor Cannabinoide CB1/metabolismo , Somatostatina/metabolismo , Animales , Biomarcadores , Regulación Enzimológica de la Expresión Génica , Genes Reporteros , Inmunohistoquímica , Ratones , Ratones Noqueados
2.
J Neurosci ; 37(45): 10943-10954, 2017 11 08.
Artículo en Inglés | MEDLINE | ID: mdl-29038246

RESUMEN

The VTA is necessary for reward behavior with dopamine cells critically involved in reward signaling. Dopamine cells in turn are innervated and regulated by neighboring inhibitory GABA cells. Using whole-cell electrophysiology in juvenile-adolescent GAD67-GFP male mice, we examined excitatory plasticity in fluorescent VTA GABA cells. A novel CB1-dependent LTD was induced in GABA cells that was dependent on metabotropic glutamate receptor 5, and cannabinoid receptor 1 (CB1). LTD was absent in CB1 knock-out mice but preserved in heterozygous littermates. Bath applied Δ9-tetrahydrocannabinol depressed GABA cell activity, therefore downstream dopamine cells will be disinhibited; and thus, this could potentially result in increased reward. Chronic injections of Δ9-tetrahydrocannabinol occluded LTD compared with vehicle injections; however, a single exposure was insufficient to do so. As synaptic modifications by drugs of abuse are often tied to addiction, these data suggest a possible mechanism for the addictive effects of Δ9-tetrahydrocannabinol in juvenile-adolescents, by potentially altering reward behavioral outcomes.SIGNIFICANCE STATEMENT The present study identifies a novel form of glutamatergic synaptic plasticity in VTA GABA neurons, a currently understudied cell type that is critical for the brain's reward circuit, and how Δ9-tetrahydrocannabinol occludes this plasticity. This study specifically addresses a potential unifying mechanism whereby marijuana could exert rewarding and addictive/withdrawal effects. Marijuana use and legalization are a pressing issue for many states in the United States. Although marijuana is the most commonly abused illicit drug, the implications of legalized, widespread, or continued usage are speculative. This study in juvenile-adolescent aged mice identifies a novel form of synaptic plasticity in VTA GABA cells, and the synaptic remodeling that can occur after Δ9-tetrahydrocannabinol use.


Asunto(s)
Cannabis , Plasticidad Neuronal/efectos de los fármacos , Neuronas/efectos de los fármacos , Receptor Cannabinoide CB1/efectos de los fármacos , Área Tegmental Ventral/efectos de los fármacos , Ácido gamma-Aminobutírico/metabolismo , Animales , Neuronas Dopaminérgicas/efectos de los fármacos , Dronabinol/farmacología , Glutamato Descarboxilasa/genética , Masculino , Ratones , Ratones Noqueados , Plasticidad Neuronal/genética , Técnicas de Placa-Clamp , Receptor Cannabinoide CB1/genética , Recompensa
3.
Addict Biol ; 23(5): 1079-1093, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-28901722

RESUMEN

Dopamine (DA) neuron excitability is regulated by inhibitory GABAergic synaptic transmission and modulated by nicotinic acetylcholine receptors (nAChRs). The aim of this study was to evaluate the role of α6 subunit-containing nAChRs (α6*-nAChRs) in acute ethanol effects on ventral tegmental area (VTA) GABA and DA neurons. α6*-nAChRs were visualized on GABA terminals on VTA GABA neurons, and α6*-nAChR transcripts were expressed in most DA neurons, but only a minority of VTA GABA neurons from GAD67 GFP mice. Low concentrations of ethanol (1-10 mM) enhanced GABAA receptor (GABAA R)-mediated spontaneous and evoked inhibition with blockade by selective α6*-nAChR antagonist α-conotoxins (α-Ctxs) and lowered sensitivity in α6 knock-out (KO) mice. Ethanol suppression of VTA GABA neuron firing rate in wild-type mice in vivo was significantly reduced in α6 KO mice. Ethanol (5-100 mM) had no effect on optically evoked GABAA R-mediated inhibition of DA neurons, and ethanol enhancement of VTA DA neuron firing rate at high concentrations was not affected by α-Ctxs. Ethanol conditioned place preference was reduced in α6 KO mice compared with wild-type controls. Taken together, these studies indicate that relatively low concentrations of ethanol act through α6*-nAChRs on GABA terminals to enhance GABA release onto VTA GABA neurons, in turn to reduce GABA neuron firing, which may lead to VTA DA neuron disinhibition, suggesting a possible mechanism of action of alcohol and nicotine co-abuse.


Asunto(s)
Etanol/farmacología , Neuronas GABAérgicas/efectos de los fármacos , Receptores Nicotínicos/metabolismo , Recompensa , Área Tegmental Ventral/efectos de los fármacos , Animales , Etanol/metabolismo , Neuronas GABAérgicas/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Modelos Animales , Transmisión Sináptica/efectos de los fármacos , Área Tegmental Ventral/metabolismo
4.
Hippocampus ; 27(9): 985-998, 2017 09.
Artículo en Inglés | MEDLINE | ID: mdl-28653801

RESUMEN

GPR55, an orphan G-protein coupled receptor, is activated by lysophosphatidylinositol (LPI) and the endocannabinoid anandamide, as well as by other compounds including THC. LPI is a potent endogenous ligand of GPR55 and neither GPR55 nor LPIs' functions in the brain are well understood. While endocannabinoids are well known to modulate brain synaptic plasticity, the potential role LPI could have on brain plasticity has never been demonstrated. Therefore, we examined not only GPR55 expression, but also the role its endogenous ligand could play in long-term potentiation, a common form of synaptic plasticity. Using quantitative RT-PCR, electrophysiology, and behavioral assays, we examined hippocampal GPR55 expression and function. qRT-PCR results indicate that GPR55 is expressed in hippocampi of both rats and mice. Immunohistochemistry and single cell PCR demonstrates GPR55 protein in pyramidal cells of CA1 and CA3 layers in the hippocampus. Application of the GPR55 endogenous agonist LPI to hippocampal slices of GPR55+/+ mice significantly enhanced CA1 LTP. This effect was absent in GPR55-/- mice, and blocked by the GPR55 antagonist CID 16020046. We also examined paired-pulse ratios of GPR55-/- and GPR55+/+ mice with or without LPI and noted significant enhancement in paired-pulse ratios by LPI in GPR55+/+ mice. Behaviorally, GPR55-/- and GPR55+/+ mice did not differ in memory tasks including novel object recognition, radial arm maze, or Morris water maze. However, performance on radial arm maze and elevated plus maze task suggests GPR55-/- mice have a higher frequency of immobile behavior. This is the first demonstration of LPI involvement in hippocampal synaptic plasticity.


Asunto(s)
Potenciales Postsinápticos Excitadores/genética , Regulación de la Expresión Génica/genética , Hipocampo/citología , Hipocampo/fisiología , Receptores de Cannabinoides/metabolismo , Animales , Animales Recién Nacidos , Compuestos de Azabiciclo/farmacología , Benzoatos/farmacología , Estimulación Eléctrica , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Regulación de la Expresión Génica/efectos de los fármacos , Glutamato Descarboxilasa/genética , Glutamato Descarboxilasa/metabolismo , Ácido Glutámico/farmacología , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Lisofosfolípidos/farmacología , Masculino , Aprendizaje por Laberinto/fisiología , Ratones , Ratones Transgénicos , Técnicas de Placa-Clamp , Ratas , Ratas Sprague-Dawley , Receptores de Cannabinoides/genética , Reconocimiento en Psicología/fisiología
5.
Neurochem Int ; 145: 105002, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-33617930

RESUMEN

The ventral tegmental area (VTA) in the midbrain is essential in incentive salience of reward behavior. Drugs of abuse increase midbrain dopamine cell activity and/or dopamine levels, and can alter endogenous VTA glutamate plasticity, leading to addiction or dependence. VTA dopamine cells are regulated by local inhibitory GABA cells, which exhibit a form of pre-synaptic cannabinoid receptor 1-dependent long-term depression of their glutamatergic inputs. Our current aim was to determine cocaine's influence on VTA GABA cell glutamate plasticity and circuity. Using whole cell voltage-clamp electrophysiology in VTA slices of GAD67-GFP knock-in mice, we recorded excitatory inputs on VTA GABA cells. Acute and chronic injections of cocaine were sufficient to occlude long-term depression. The plasticity could be reversed to the naïve state however, as long-term depression was again observed following a 7-day abstinence from acute cocaine exposure. Furthermore, chronic cocaine decreased AMPA/NMDA ratios at glutamate synapses onto VTA GABA cells, compared to vehicle injection controls, the opposite change noted in dopamine cells. Collectively, our data suggest the cellular mechanism of cocaine-mediated synaptic modification that may result in dependence/withdrawal could involve changes in glutamate input to VTA GABA circuitry in addition to VTA dopamine cells. Therefore VTA GABA cells may also play a role, possibly in a synergistic manner with the dopamine circuit, in cocaine-induced changes to the VTA reward pathway than previously known.


Asunto(s)
Cocaína/administración & dosificación , Inhibidores de Captación de Dopamina/administración & dosificación , Neuronas GABAérgicas/efectos de los fármacos , Depresión Sináptica a Largo Plazo/efectos de los fármacos , Área Tegmental Ventral/efectos de los fármacos , Animales , Femenino , Neuronas GABAérgicas/fisiología , Técnicas de Sustitución del Gen/métodos , Depresión Sináptica a Largo Plazo/fisiología , Masculino , Ratones , Ratones Transgénicos , Área Tegmental Ventral/fisiología
6.
Neurosci Lett ; 712: 134472, 2019 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-31499135

RESUMEN

Changes in synaptic strength between hippocampal CA1 pyramidal cell synapses are partly responsible for memory acquisition. This plasticity is modulated by feedforward inhibitory interneurons in the stratum radiatum. While radiatum interneurons experience either long-term depression (LTD), short-term depression (STD), or lack plasticity, it is unclear whether plasticity correlates to specific interneuron subtypes. Using whole-cell electrophysiology and real-time quantitative PCR, we characterized the plasticity expressed by different interneuron subtypes. We first analyzed calcium binding proteins and cholecystokinin mRNA expression patterns to determine cell subtype. We then assessed endocannabinoid (eCB) biosynthetic enzyme mRNA expression, including diacylglycerol lipase α, N-acyl-phosphatidylethanolamine phospholipase D, and 12-lipoxygenase, and metabotropic glutamate receptors that often mediate plasticity. Neurons exhibiting LTD tended to co-express mRNA for at least one eCB biosynthetic enzyme and the metabotropic glutamate receptor 5 (mGluR5). Conversely, mGluR5 was not expressed by neurons exhibiting STD or no plasticity. Neurons that exhibited STD tended to express mRNA for at least one eCB biosynthetic enzyme and mGluR1, but not mGluR5. This suggests that plasticity of stratum radiatum interneurons could be predicted based on type I mGluR expression.


Asunto(s)
Hipocampo/metabolismo , Interneuronas/metabolismo , Plasticidad Neuronal/fisiología , Receptores de Glutamato Metabotrópico/metabolismo , Animales , Proteínas de Unión al Calcio/metabolismo , Colecistoquinina/metabolismo , Lipoproteína Lipasa/metabolismo , Masculino , Técnicas de Placa-Clamp , Fosfolipasa D/metabolismo , Ratas , Ratas Sprague-Dawley , Receptor del Glutamato Metabotropico 5/metabolismo , Sinapsis/fisiología , Transmisión Sináptica/fisiología
7.
Sci Rep ; 7: 46464, 2017 04 18.
Artículo en Inglés | MEDLINE | ID: mdl-28418030

RESUMEN

The novel nuclear protein nBMP2 is synthesized from the BMP2 gene by translational initiation at an alternative start codon. We generated a targeted mutant mouse, nBmp2NLStm, in which the nuclear localization signal (NLS) was inactivated to prevent nuclear translocation of nBMP2 while still allowing the normal synthesis and secretion of the BMP2 growth factor. These mice exhibit abnormal muscle function due to defective Ca2+ transport in skeletal muscle. We hypothesized that neurological function, which also depends on intracellular Ca2+ transport, could be affected by the loss of nBMP2. Age-matched nBmp2NLStm and wild type mice were analyzed by immunohistochemistry, behavioral tests, and electrophysiology to assess nBMP2 expression and neurological function. Immunohistochemical staining of the hippocampus detected nBMP2 in the nuclei of CA1 neurons in wild type but not mutant mice, consistent with nBMP2 playing a role in the hippocampus. Mutant mice showed deficits in the novel object recognition task, suggesting hippocampal dysfunction. Electrophysiology experiments showed that long-term potentiation (LTP) in the hippocampus, which is dependent on intracellular Ca2+ transport and is thought to be the cellular equivalent of learning and memory, was impaired. Together, these results suggest that nBMP2 in the hippocampus impacts memory formation.


Asunto(s)
Proteína Morfogenética Ósea 2/genética , Proteína Morfogenética Ósea 2/metabolismo , Región CA1 Hipocampal/metabolismo , Memoria/fisiología , Empalme Alternativo , Animales , Proteína Morfogenética Ósea 2/química , Región CA1 Hipocampal/fisiología , Calcio/metabolismo , Núcleo Celular/metabolismo , Codón Iniciador , Potenciación a Largo Plazo , Masculino , Ratones , Mutación , Señales de Localización Nuclear
8.
Sci Rep ; 5: 16176, 2015 Nov 10.
Artículo en Inglés | MEDLINE | ID: mdl-26553597

RESUMEN

The ventral tegmental area (VTA) is involved in adaptive reward and motivation processing and is composed of dopamine (DA) and GABA neurons. Defining the elements regulating activity and synaptic plasticity of these cells is critical to understanding mechanisms of reward and addiction. While endocannabinoids (eCBs) that potentially contribute to addiction are known to be involved in synaptic plasticity mechanisms in the VTA, where they are produced is poorly understood. In this study, DA and GABAergic cells were identified using electrophysiology, cellular markers, and a transgenic mouse model that specifically labels GABA cells. Using single-cell RT-qPCR and immunohistochemistry, we investigated mRNA and proteins involved in eCB signaling such as diacylglycerol lipase α, N-acyl-phosphatidylethanolamine-specific phospholipase D, and 12-lipoxygenase, as well as type I metabotropic glutamate receptors (mGluRs). Our results demonstrate the first molecular evidence of colocalization of eCB biosynthetic enzyme and type I mGluR mRNA in VTA neurons. Further, these data reveal higher expression of mGluR1 in DA neurons, suggesting potential differences in eCB synthesis between DA and GABA neurons. These data collectively suggest that VTA GABAergic and DAergic cells have the potential to produce various eCBs implicated in altering neuronal activity or plasticity in adaptive motivational reward or addiction.


Asunto(s)
Dopamina/metabolismo , Endocannabinoides/biosíntesis , Neuronas GABAérgicas/metabolismo , Regulación de la Expresión Génica/fisiología , Receptores de Glutamato Metabotrópico/genética , Área Tegmental Ventral/metabolismo , Animales , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Fenómenos Electrofisiológicos , Masculino , Ratones , Ratones Transgénicos , Plasticidad Neuronal , Neuropéptidos/genética , Neuropéptidos/metabolismo , ARN Mensajero/metabolismo , Ratas , Ratas Sprague-Dawley , Receptores de Glutamato Metabotrópico/metabolismo , Transcriptoma
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