RESUMEN
Adult neural stem/progenitor (B1) cells within the walls of the lateral ventricles generate different types of neurons for the olfactory bulb (OB). The location of B1 cells determines the types of OB neurons they generate. Here we show that the majority of mouse B1 cell precursors are produced between embryonic days (E) 13.5 and 15.5 and remain largely quiescent until they become reactivated postnatally. Using a retroviral library carrying over 100,000 genetic tags, we found that B1 cells share a common progenitor with embryonic cells of the cortex, striatum, and septum, but this lineage relationship is lost before E15.5. The regional specification of B1 cells is evident as early as E11.5 and is spatially linked to the production of neurons that populate different areas of the forebrain. This study reveals an early embryonic regional specification of postnatal neural stem cells and the lineage relationship between them and embryonic progenitor cells.
Asunto(s)
Células Madre Adultas/citología , Linaje de la Célula , Embrión de Mamíferos/citología , Células-Madre Neurales/citología , Bulbo Olfatorio/citología , Células Madre Adultas/clasificación , Animales , Ratones , Células-Madre Neurales/clasificación , Prosencéfalo/citologíaRESUMEN
Canonical Wnt signaling requires inhibition of Glycogen Synthase Kinase 3 (GSK3) activity, but the molecular mechanism by which this is achieved remains unclear. Here, we report that Wnt signaling triggers the sequestration of GSK3 from the cytosol into multivesicular bodies (MVBs), so that this enzyme becomes separated from its many cytosolic substrates. Endocytosed Wnt colocalized with GSK3 in acidic vesicles positive for endosomal markers. After Wnt addition, endogenous GSK3 activity decreased in the cytosol, and GSK3 became protected from protease treatment inside membrane-bounded organelles. Cryoimmunoelectron microscopy showed that these corresponded to MVBs. Two proteins essential for MVB formation, HRS/Vps27 and Vps4, were required for Wnt signaling. The sequestration of GSK3 extended the half-life of many other proteins in addition to ß-Catenin, including an artificial Wnt-regulated reporter protein containing GSK3 phosphorylation sites. We conclude that multivesicular endosomes are essential components of the Wnt signal-transduction pathway.
Asunto(s)
Glucógeno Sintasa Quinasa 3/metabolismo , Cuerpos Multivesiculares/metabolismo , Transducción de Señal , Proteínas Wnt/metabolismo , Animales , Línea Celular , Embrión no Mamífero/metabolismo , Humanos , Ratones , Cuerpos Multivesiculares/ultraestructura , Fosforilación , Estabilidad Proteica , XenopusRESUMEN
The transcriptional control of primary cilium formation and ciliary motility are beginning to be understood, but little is known about the transcriptional programs that control cilium number and other structural and functional specializations. One of the most intriguing ciliary specializations occurs in multiciliated cells (MCCs), which amplify their centrioles to nucleate hundreds of cilia per cell, instead of the usual monocilium. Here we report that the transcription factor MYB, which promotes S phase and drives cycling of a variety of progenitor cells, is expressed in postmitotic epithelial cells of the mouse airways and ependyma destined to become MCCs. MYB is expressed early in multiciliogenesis, as progenitors exit the cell cycle and amplify their centrioles, then switches off as MCCs mature. Conditional inactivation of Myb in the developing airways blocks or delays centriole amplification and expression of FOXJ1, a transcription factor that controls centriole docking and ciliary motility, and airways fail to become fully ciliated. We provide evidence that MYB acts in a conserved pathway downstream of Notch signaling and multicilin, a protein related to the S-phase regulator geminin, and upstream of FOXJ1. MYB can activate endogenous Foxj1 expression and stimulate a cotransfected Foxj1 reporter in heterologous cells, and it can drive the complete multiciliogenesis program in Xenopus embryonic epidermis. We conclude that MYB has an early, crucial and conserved role in multiciliogenesis, and propose that it promotes a novel S-like phase in which centriole amplification occurs uncoupled from DNA synthesis, and then drives later steps of multiciliogenesis through induction of Foxj1.
Asunto(s)
Centriolos/metabolismo , Cilios/metabolismo , Factores de Transcripción Forkhead/metabolismo , Proteínas Proto-Oncogénicas c-myb/metabolismo , Animales , Encéfalo/embriología , Encéfalo/metabolismo , Diferenciación Celular , Movimiento Celular , Células Cultivadas , Centriolos/genética , Cilios/genética , Epéndimo/embriología , Epéndimo/metabolismo , Células Epiteliales/metabolismo , Factores de Transcripción Forkhead/biosíntesis , Pulmón/embriología , Pulmón/metabolismo , Ratones/embriología , Ratones Transgénicos , Transducción de Señal , Tráquea/embriología , Tráquea/metabolismo , Xenopus laevis/embriologíaRESUMEN
The intensity of the BMP signal is determined by cell surface receptors that phosphorylate Smad1/5/8 at the C-terminus. In addition to this BMP-activated phosphorylation, recent studies have shown that sequential phosphorylations by MAPK and GSK3 kinases can negatively regulate the activity of the pSmad1Cter signal. These phosphorylations in the linker region cause Smad1 to be transported to the centrosomal region, polyubiquitinylated and degraded by the proteasomal machinery. In Xenopus embryos, Wnt signals, which regulate GSK3, induce ectoderm to adopt an epidermal fate, and this Wnt effect requires an active BMP-Smad1/5/8 signaling pathway. These findings have profound implications for understanding how dorsal-ventral and anterior-posterior patterning are seamlessly integrated in the early embryonic morphogenetic field.
Asunto(s)
Tipificación del Cuerpo/fisiología , Proteína Smad1/fisiología , Proteína Smad5/fisiología , Proteína Smad8/fisiología , Secuencia de Aminoácidos , Animales , Tipificación del Cuerpo/genética , Embrión no Mamífero , Humanos , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Proteínas Quinasas Activadas por Mitógenos/fisiología , Modelos Biológicos , Datos de Secuencia Molecular , Fosforilación , Transducción de Señal , Proteína Smad1/antagonistas & inhibidores , Proteína Smad1/genética , Proteína Smad1/metabolismo , Proteína Smad5/genética , Proteína Smad8/genética , Xenopus/embriología , Xenopus/genéticaRESUMEN
Mitotic cell division ensures that two daughter somatic cells inherit identical genetic material. Previous work has shown that signaling by the Smad1 transcription factor is terminated by polyubiquitinylation and proteasomal degradation after essential phosphorylations by MAPK and glycogen synthase kinase 3 (GSK3). Here, we show that, unexpectedly, proteins specifically targeted for proteasomal degradation are inherited preferentially by one mitotic daughter during somatic cell division. Experiments with dividing human embryonic stem cells and other mammalian cultured cell lines demonstrated that in many supposedly equal mitoses the segregation of proteins destined for degradation (Smad1 phosphorylated by MAPK and GSK3, phospho-beta-catenin, and total polyubiquitinylated proteins) was asymmetric. Transport of pSmad1 targeted for degradation to the centrosome required functional microtubules. In vivo, an antibody specific for Mad phosphorylated by MAPK showed that this antigen was associated preferentially with one of the two centrosomes in Drosophila embryos at cellular blastoderm stage. We propose that this remarkable cellular property may be explained by the asymmetric inheritance of peripheral centrosomal proteins when centrioles separate and migrate to opposite poles of the cell, so that one mitotic daughter remains pristine. We conclude that many mitotic divisions are unequal, unlike what was previously thought.
Asunto(s)
Mitosis , Poliubiquitina/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Proteínas/metabolismo , Ubiquitinación , Animales , Blastodermo/citología , Blastodermo/metabolismo , Proteínas Morfogenéticas Óseas/metabolismo , Células COS/metabolismo , Línea Celular , Centrosoma/metabolismo , Chlorocebus aethiops , Drosophila/citología , Drosophila/embriología , Drosophila/metabolismo , Células Madre Embrionarias/citología , Células Madre Embrionarias/metabolismo , Glucógeno Sintasa Quinasa 3/metabolismo , Humanos , Microtúbulos/metabolismo , Quinasas de Proteína Quinasa Activadas por Mitógenos/metabolismo , Modelos Biológicos , Fosforilación , Transporte de Proteínas , Proteína Smad1/metabolismo , beta Catenina/metabolismoRESUMEN
The adult mouse brain contains an extensive neurogenic niche in the lateral walls of the lateral ventricles. This epithelium, which has a unique pinwheel organization, contains multiciliated ependymal (E1) cells and neural stem cells (B1). This postnatal germinal epithelium develops from the embryonic ventricular zone, but the lineage relationship between E1 and B1 cells remains unknown. Distinct subpopulations of radial glia (RG) cells in late embryonic and early postnatal development either expand their apical domain >11-fold to form E1 cells or retain small apical domains that coalesce into the centers of pinwheels to form B1 cells. Using independent methods of lineage tracing, we show that individual RG cells can give rise to clones containing E1 and B1 cells. This study reveals key developmental steps in the formation of the postnatal germinal niche and the shared cellular origin of E1 and B1 cells.
Asunto(s)
Epéndimo/embriología , Células-Madre Neurales/metabolismo , Neurogénesis/genética , Animales , Humanos , RatonesRESUMEN
The pons controls crucial sensorimotor and autonomic functions. In humans, it grows sixfold postnatally and is a site of paediatric gliomas; however, the mechanisms of pontine growth remain poorly understood. We show that the murine pons quadruples in volume postnatally; growth is fastest during postnatal days 0-4 (P0-P4), preceding most myelination. We identify three postnatal proliferative compartments: ventricular, midline and parenchymal. We find no evidence of postnatal neurogenesis in the pons, but each progenitor compartment produces new astroglia and oligodendroglia; the latter expand 10- to 18-fold postnatally, and are derived mostly from the parenchyma. Nearly all parenchymal progenitors at P4 are Sox2(+)Olig2(+), but by P8 a Sox2(-) subpopulation emerges, suggesting a lineage progression from Sox2(+) 'early' to Sox2(-) 'late' oligodendrocyte progenitor. Fate mapping reveals that >90% of adult oligodendrocytes derive from P2-P3 Sox2(+) progenitors. These results demonstrate the importance of postnatal Sox2(+)Olig2(+) progenitors in pontine growth and oligodendrogenesis.
Asunto(s)
Células Precursoras de Oligodendrocitos/fisiología , Puente/crecimiento & desarrollo , Animales , Animales Recién Nacidos/crecimiento & desarrollo , Proliferación Celular , Cuarto Ventrículo/citología , Ratones , Neurogénesis , Factor de Transcripción 2 de los Oligodendrocitos/metabolismo , Oligodendroglía/fisiología , Puente/citología , Factores de Transcripción SOXB1/metabolismoRESUMEN
Neural stem cells in different locations of the postnatal mouse ventricular-subventricular zone (V-SVZ) generate different subtypes of olfactory bulb (OB) interneurons. High Sonic hedgehog (SHH) signaling in the ventral V-SVZ regulates the production of specific subtypes of neurons destined for the OB. Here we found a transient territory of high SHH signaling in the dorsal V-SVZ beneath the corpus callosum (CC). Using intersectional lineage tracing in neonates to label dorsal radial glial cells (RGCs) expressing the SHH target gene Gli1, we demonstrate that this region produces many CC cells in the oligodendroglial lineage and specific subtypes of neurons in the OB. The number of oligodendroglial cells generated correlated with the levels of SHH signaling. This work identifies a dorsal domain of SHH signaling, which is an important source of oligodendroglial cells for the postnatal mammalian forebrain.
Asunto(s)
Encéfalo/crecimiento & desarrollo , Proteínas Hedgehog/metabolismo , Células-Madre Neurales/citología , Bulbo Olfatorio/citología , Oligodendroglía/citología , Transducción de Señal , Animales , Encéfalo/citología , Encéfalo/metabolismo , Linaje de la Célula , Cuerpo Calloso/citología , Cuerpo Calloso/crecimiento & desarrollo , Cuerpo Calloso/metabolismo , Expresión Génica , Factores de Transcripción de Tipo Kruppel/genética , Factores de Transcripción de Tipo Kruppel/metabolismo , Ratones , Células-Madre Neurales/metabolismo , Bulbo Olfatorio/crecimiento & desarrollo , Bulbo Olfatorio/metabolismo , Oligodendroglía/metabolismo , Proteína con Dedos de Zinc GLI1RESUMEN
The mammalian neocortex is composed of two major neuronal cell types with distinct origins: excitatory pyramidal neurons and inhibitory interneurons, generated in dorsal and ventral progenitor zones of the embryonic telencephalon, respectively. Thus, inhibitory neurons migrate relatively long distances to reach their destination in the developing forebrain. The role of lineage in the organization and circuitry of interneurons is still not well understood. Utilizing a combination of genetics, retroviral fate mapping, and lineage-specific retroviral barcode labeling, we find that clonally related interneurons can be widely dispersed while unrelated interneurons can be closely clustered. These data suggest that migratory mechanisms related to the clustering of interneurons occur largely independent of their clonal origin.
Asunto(s)
Movimiento Celular/fisiología , Interneuronas/fisiología , Red Nerviosa/fisiología , Inhibición Neural , Células-Madre Neurales/fisiología , Telencéfalo/citología , Animales , Linaje de la Célula , Células Cultivadas , Corteza Cerebral/citología , Embrión de Mamíferos , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Técnicas In Vitro , Captura por Microdisección con Láser , Ratones , Proteínas del Tejido Nervioso/metabolismo , Células-Madre Neurales/clasificación , Técnicas de Cultivo de Órganos , Telencéfalo/embriología , Transducción GenéticaRESUMEN
Astrocytes have many roles within the brain parenchyma, and a subpopulation restricted to germinal niches functions as neural stem cells (NSCs) that produce various types of neuronal progeny in relation to spatiotemporal factors. A growing body of evidence supports the concept of morphological and molecular differences between astrocytes in different brain regions, which might relate to their derivation from regionally patterned radial glia. Indeed, the notion that astrocytes are molecularly and functionally heterogeneous could help explain how the central nervous system (CNS) retains embryonic positional information into adulthood. Here, we discuss recent evidence for regionally encoded functions of astrocytes in the developing and adult CNS to provide an integrated concept of the origin and possible function of astrocyte heterogeneity. We focus on the regionalization of NSCs in the ventricular-subventricular zone (V-SVZ) of the adult mammalian brain and emerging evidence for a segmental organization of astrocytes in the developing spinal cord and forebrain. We propose that astrocytes' diversity will provide fundamental clues to understand regional brain organization and function.
Asunto(s)
Astrocitos/fisiología , Ventrículos Cerebrales/embriología , Mamíferos/fisiología , Modelos Neurológicos , Células-Madre Neurales/fisiología , Neuroglía/fisiología , Médula Espinal/citología , Adulto , Animales , Ventrículos Cerebrales/citología , Humanos , Neuroglía/citologíaRESUMEN
Throughout life, neural stem cells (NSCs) in different domains of the ventricular-subventricular zone (V-SVZ) of the adult rodent brain generate several subtypes of interneurons that regulate the function of the olfactory bulb. The full extent of diversity among adult NSCs and their progeny is not known. Here, we report the generation of at least four previously unknown olfactory bulb interneuron subtypes that are produced in finely patterned progenitor domains in the anterior ventral V-SVZ of both the neonatal and adult mouse brain. Progenitors of these interneurons are responsive to sonic hedgehog and are organized into microdomains that correlate with the expression domains of the Nkx6.2 and Zic family of transcription factors. This work reveals an unexpected degree of complexity in the specification and patterning of NSCs in the postnatal mouse brain.
Asunto(s)
Interneuronas/clasificación , Interneuronas/fisiología , Microdominios de Membrana/fisiología , Células-Madre Neurales/citología , Factores de Edad , Animales , Ventrículos Cerebrales/citología , Ventrículos Cerebrales/crecimiento & desarrollo , Regulación del Desarrollo de la Expresión Génica/genética , Regulación del Desarrollo de la Expresión Génica/fisiología , Proteína Ácida Fibrilar de la Glía/genética , Proteína Ácida Fibrilar de la Glía/metabolismo , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Proteínas de Homeodominio/genética , Proteínas de Homeodominio/metabolismo , Factores de Transcripción de Tipo Kruppel/metabolismo , Ratones , Neuroglía/fisiología , ARN no Traducido/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Transducción Genética , Proteína con Dedos de Zinc GLI1RESUMEN
Major developments in the neural stem cell (NSC) field in recent years provide new insights into the nature of the NSC niche. In this perspective, we integrate recent anatomical data on the organization of the two main neurogenic niches in the adult brain, the ventricular-subventricular zone (V-SVZ) and the subgranular zone (SGZ), with signaling pathways that control the behavior of NSCs. NSCs in the adult brain stretch into physiologically distinct compartments of their niche. We propose how adult NSCs' morphology may allow these cells to integrate multiple signaling pathways arising from unique locations of their niche.
Asunto(s)
Células Madre Adultas/citología , Encéfalo/citología , Células-Madre Neurales/citología , Neuronas/citología , Nicho de Células Madre , Adulto , Células Madre Adultas/fisiología , Humanos , Células-Madre Neurales/fisiología , Transducción de SeñalRESUMEN
Astrocytes, the most abundant cell population in the central nervous system (CNS), are essential for normal neurological function. We show that astrocytes are allocated to spatial domains in mouse spinal cord and brain in accordance with their embryonic sites of origin in the ventricular zone. These domains remain stable throughout life without evidence of secondary tangential migration, even after acute CNS injury. Domain-specific depletion of astrocytes in ventral spinal cord resulted in abnormal motor neuron synaptogenesis, which was not rescued by immigration of astrocytes from adjoining regions. Our findings demonstrate that region-restricted astrocyte allocation is a general CNS phenomenon and reveal intrinsic limitations of the astroglial response to injury.
Asunto(s)
Astrocitos/fisiología , Encéfalo/citología , Movimiento Celular , Neuronas Motoras/fisiología , Médula Espinal/citología , Sinapsis/fisiología , Animales , Proteínas Bacterianas/metabolismo , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Encéfalo/anomalías , Encéfalo/fisiología , Lesiones Encefálicas/fisiopatología , Proteínas Fluorescentes Verdes , Proteína Homeobox Nkx-2.2 , Proteínas de Homeodominio/metabolismo , Integrasas/genética , Proteínas Luminiscentes/metabolismo , Ratones , Ratones Transgénicos , Proteínas del Tejido Nervioso/genética , Proteínas/metabolismo , ARN no Traducido , Médula Espinal/anomalías , Médula Espinal/fisiología , Traumatismos de la Médula Espinal/fisiopatología , Factores de Transcripción/metabolismo , Transcripción Genética , Proteínas de Pez CebraRESUMEN
A key question in developmental biology is how growth factor signals are integrated to generate pattern. In this study we investigated the integration of the Drosophila BMP and Wingless/GSK3 signaling pathways via phosphorylations of the transcription factor Mad. Wingless was found to regulate the phosphorylation of Mad by GSK3 in vivo. In epistatic experiments, the effects of Wingless on wing disc molecular markers (senseless, distalless and vestigial) were suppressed by depletion of Mad with RNAi. Wingless overexpression phenotypes, such as formation of ectopic wing margins, were induced by Mad GSK3 phosphorylation-resistant mutant protein. Unexpectedly, we found that Mad phosphorylation by GSK3 and MAPK occurred in segmental patterns. Mad depletion or overexpression produced Wingless-like embryonic segmentation phenotypes. In Xenopus embryos, segmental border formation was disrupted by Smad8 depletion. The results show that Mad is required for Wingless signaling and for the integration of gradients of positional information.
Asunto(s)
Tipificación del Cuerpo , Proteínas de Unión al ADN/fisiología , Proteínas de Drosophila/metabolismo , Proteínas de Drosophila/fisiología , Drosophila/embriología , Transducción de Señal/fisiología , Factores de Transcripción/fisiología , Alas de Animales/embriología , Proteína Wnt1/metabolismo , Animales , Proteínas de Unión al ADN/genética , Proteínas de Drosophila/genética , Mutación , Fenotipo , Interferencia de ARN , Factores de Transcripción/genéticaRESUMEN
BMP receptors determine the intensity of BMP signals via Smad1 C-terminal phosphorylations. Here we show that a finely controlled cell biological pathway terminates this activity. The duration of the activated pSmad1(Cter) signal was regulated by sequential Smad1 linker region phosphorylations at conserved MAPK and GSK3 sites required for its polyubiquitinylation and transport to the centrosome. Proteasomal degradation of activated Smad1 and total polyubiquitinated proteins took place in the centrosome. Inhibitors of the Erk, p38, and JNK MAPKs, as well as GSK3 inhibitors, prolonged the duration of a pulse of BMP7. Wnt signaling decreased pSmad1(GSK3) antigen levels and redistributed it from the centrosome to cytoplasmic LRP6 signalosomes. In Xenopus embryos, it was found that Wnts induce epidermis and that this required an active BMP-Smad pathway. Epistatic experiments suggested that the dorsoventral (BMP) and anteroposterior (Wnt/GSK3) patterning gradients are integrated at the level of Smad1 phosphorylations during embryonic pattern formation.