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This chapter covers the fundamental aspects of bacterial S-layers: what are S-layers, what is known about them, and what are their main features that makes them so interesting for the production of nanostructures. After a detailed introduction of the paracrystalline protein lattices formed by S-layer systems in nature the chapter explores the engineering of S-layer-based materials. How can S-layers be used to produce "industry-ready" nanoscale bio-composite materials, and which kinds of nanomaterials are possible (e.g., nanoparticle synthesis, nanoparticle immobilization, and multifunctional coatings)? What are the advantages and disadvantages of S-layer-based composite materials? Finally, the chapter highlights the potential of these innovative bacterial biomolecules for future technologies in the fields of metal filtration, catalysis, and bio-functionalization.
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Bacterias/química , Glicoproteínas de Membrana/química , Nanocompuestos/químicaRESUMEN
Chemical functional groups of surface layer (S-layer) proteins were chemically modified in order to evaluate the potential of S-layer proteins for the introduction of functional molecules. S-layer proteins are structure proteins that self-assemble into regular arrays on surfaces. One general feature of S-layer proteins is their high amount of carboxylic and amino groups. These groups are potential targets for linking functional molecules, thus producing reactive surfaces. In this work, these groups were conjugated with the amino acid tryptophan. In another approach, SH-groups were chemically inserted in order to extend the spectrum of modifiable groups. The amount of modifiable carboxylic groups was further evaluated by potentiometric titration in order to evaluate the potential efficiency of S-layer proteins to work as matrix for bioconjugations. The results proved that S-layer proteins can work as effective matrices for the conjugation of different molecules. The advantage of using chemical modification methods over genetic methods lies in its versatile usage enabling the attachment of biomolecules, as well as fluorescent dyes and inorganic molecules. Together with their self-assembling properties, S-layer proteins are suitable as targets for bioconjugates, thus enabling a nanostructuring and bio-functionalization of surfaces, which can be used for different applications like biosensors, filter materials, or (bio)catalytic surfaces.
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Proteínas Bacterianas/química , Proteínas Inmovilizadas/química , Glicoproteínas de Membrana/química , Triptófano/química , Bacillaceae/química , Proteínas Bacterianas/aislamiento & purificación , Biocatálisis , Técnicas Biosensibles , Colorantes Fluorescentes/química , Proteínas Inmovilizadas/aislamiento & purificación , Glicoproteínas de Membrana/aislamiento & purificación , Nanotecnología/métodos , Potenciometría , Unión Proteica , Compuestos de Sulfhidrilo/química , Propiedades de SuperficieRESUMEN
Surface layer proteins (S-layer) of Lysinibacillus sphaericus JG-B53 are biological compounds with several bio-based technical applications such as biosorptive materials for metal removal or rare metals recovery from the environment. Despite their well-described applications, a deeper understanding of their metal sorption behavior still remains challenging. The metal sorption ability of Au(3+), Pd(2+), Pt(2+) and Eu(3+) was investigated by ICP-MS, AFM and QCM-D which enables the sorption detection in real-time during in situ experiments. Results indicate a high binding of Pd, followed by Au, Eu and Pt to the proteins. The comparison between different methods allowed a deeper understanding of the metal sorption of isolated S-layer either frees in liquid, adsorbed forming a protein layer or as the bacteria surface.
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Bacillaceae/metabolismo , Proteínas Bacterianas/química , Glicoproteínas de Membrana/química , Metales Pesados/química , Adsorción , Cristalización , Contaminantes Ambientales/química , Europio/química , Oro/química , Microscopía de Fuerza Atómica , Paladio/química , Platino (Metal)/química , Tecnicas de Microbalanza del Cristal de Cuarzo , Desintoxicación por Sorción , Espectrofotometría AtómicaRESUMEN
The numerical extraction of vortex cores from time-dependent fluid flow attracted much attention over the past decades. A commonly agreed upon vortex definition remained elusive since a proper vortex core needs to satisfy two hard constraints: it must be objective and Lagrangian. Recent methods on objectivization met the first but not the second constraint, since there was no formal guarantee that the resulting vortex coreline is indeed a pathline of the fluid flow. In this paper, we propose the first vortex core definition that is both objective and Lagrangian. Our approach restricts observer motions to follow along pathlines, which reduces the degrees of freedoms: we only need to optimize for an observer rotation that makes the observed flow as steady as possible. This optimization succeeds along Lagrangian vortex corelines and will result in a non-zero time-partial everywhere else. By performing this optimization at each point of a spatial grid, we obtain a residual scalar field, which we call vortex deviation error. The local minima on the grid serve as seed points for a gradient descent optimization that delivers sub-voxel accurate corelines. The visualization of both 2D and 3D vortex cores is based on the separation of the movement of the vortex core and the swirling flow behavior around it. While the vortex core is represented by a pathline, the swirling motion around it is visualized by streamlines in the correct frame. We demonstrate the utility of the approach on several 2D and 3D time-dependent vector fields'.
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Trajectory data consisting of a low number of smooth parametric curves are standard data sets in visualization. For a visual analysis, not only the behavior of the individual trajectories is of interest but also the relation of the trajectories to each other. Moving objects represented by the trajectories may rotate around each other or around a moving center. We present an approach to compute and visually analyze such rotational behavior in an objective way. We introduce trajectory vorticity (TRV), a measure of rotational behavior of a low number of trajectories. We show that it is objective and that it can be introduced in two independent ways: by approaches for unsteadiness minimization and by considering the relative spin tensor. We compare TRV against single-trajectory methods and apply it to a number of constructed and real trajectory data sets, including drifting buoys in the Atlantic, midge swarm tracking data, pedestrian tracking data, pigeon flocks, and a simulated vortex street.
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Lysinibacillus sphaericus JG-B53 was isolated from the uranium mining waste pile Haberland near Johanngeorgenstadt, Germany. Previous studies have shown that many bacteria that have been isolated from these heavy metal contaminated environments possess surface layer (S-layer) proteins that enable the bacteria to survive by binding metals with high affinity. Conversely, essential trace elements are able to cross the filter layer and reach the interior of the cell. This is especially true of the S-layer of L. sphaericus JG-B53, which possesses outstanding recrystallization and metal-binding properties. In this study, S-layer protein gene sequences encoded in the genome of L. sphaericus JG-B53 were identified using next-generation sequencing technology followed by bioinformatic analyses. The genome of L. sphaericus JG-B53 encodes at least eight putative S-layer protein genes with distinct differences. Using mRNA analysis the expression of the putative S-layer protein genes was studied. The functional S-layer protein B53 Slp1 was identified as the dominantly expressed S-layer protein in L. sphaericus JG-B53 by mRNA studies, SDS-PAGE and N-terminal sequencing. B53 Slp1 is characterized by square lattice symmetry and a molecular mass of 116 kDa. The S-layer protein B53 Slp1 shows a high similarity to the functional S-layer protein of L. sphaericus JG-A12, which was isolated from the same uranium mining waste pile Haberland and has been described by previous research. These similarities indicate horizontal gene transfer and DNA rearrangements between these bacteria. The presence of multiple S-layer gene copies may enable the bacterial strains to quickly adapt to changing environments.
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Bacillaceae/genética , Expresión Génica , Glicoproteínas de Membrana/genética , Bacillaceae/clasificación , Bacillaceae/aislamiento & purificación , Biología Computacional , ADN Bacteriano/química , ADN Bacteriano/genética , Microbiología Ambiental , Perfilación de la Expresión Génica , Alemania , Secuenciación de Nucleótidos de Alto Rendimiento , Glicoproteínas de Membrana/biosíntesis , Glicoproteínas de Membrana/química , Datos de Secuencia Molecular , Peso Molecular , Homología de Secuencia de AminoácidoRESUMEN
The Gaussian mixture model (GMM) describes the distribution of random variables from several different populations. GMMs have widespread applications in probability theory, statistics, machine learning for unsupervised cluster analysis and topic modeling, as well as in deep learning pipelines. So far, few efforts have been made to explore the underlying point distribution in combination with the GMMs, in particular when the data becomes high-dimensional and when the GMMs are composed of many Gaussians. We present an analysis tool comprising various GPU-based visualization techniques to explore such complex GMMs. To facilitate the exploration of high-dimensional data, we provide a novel navigation system to analyze the underlying data. Instead of projecting the data to 2D, we utilize interactive 3D views to better support users in understanding the spatial arrangements of the Gaussian distributions. The interactive system is composed of two parts: (1) raycasting-based views that visualize cluster memberships, spatial arrangements, and support the discovery of new modes. (2) overview visualizations that enable the comparison of Gaussians with each other, as well as small multiples of different choices of basis vectors. Users are supported in their exploration with customization tools and smooth camera navigations. Our tool was developed and assessed by five domain experts, and its usefulness was evaluated with 23 participants. To demonstrate the effectiveness, we identify interesting features in several data sets.
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Previous microarray analyses revealed that in Bradyrhizobium japonicum, about 100 genes are induced by genistein, an isoflavonoid secreted by soybean. This includes the three genes freC, freA, and freB (systematic designations bll4319, bll4320, and bll4321), which are likely to form a genistein-, daidzein-, and coumestrol-inducible operon and to encode a multidrug efflux system. Upstream of freCAB and in the opposite orientation, FrrA (systematic designation Blr4322), which has similarity to TetR-type regulators, is encoded. A deletion of frrA leads to increased expression of freB in the absence of an inducer. We identified the correct translational start codon of frrA and showed that the gene is inducible by genistein and daidzein. The protein, which was heterologously expressed and purified from Escherichia coli, binds to two palindrome-like DNA elements (operator A and operator B), which are located in the intergenic region between freC and frrA. The replacement of several nucleotides or the insertion of additional spacer nucleotides prevented binding. Binding of FrrA was also affected by the addition of genistein. By mapping the transcription start sites, we found that operator A covers the transcriptional start site of freC and operator B is probably located between the -35 regions of the two divergently oriented genes. Operator A seems to be conserved in a few similar gene constellations in other proteobacteria. Our data indicate that in B. japonicum, besides NodD1 (the LysR family) and NodVW (a two-component response regulator), a third regulator type (a TetR family member) which responds to the plant signal molecules genistein and daidzein exists.
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Proteínas Bacterianas/metabolismo , Bradyrhizobium/metabolismo , Flavonoides/farmacología , Regulación Bacteriana de la Expresión Génica/fisiología , Proteínas Bacterianas/genética , Bradyrhizobium/efectos de los fármacos , Bradyrhizobium/genética , Codón Iniciador , Mutación , Nodulación de la Raíz de la Planta , Glycine max/microbiología , Glycine max/fisiologíaRESUMEN
BACKGROUND: Escherichia coli is one of the best studied microorganisms and finds multiple applications especially as tool in the heterologous production of interesting proteins of other organisms. The heterologous expression of special surface (S-) layer proteins caused the formation of extremely long E. coli cells which leave transparent tubes when they divide into single E. coli cells. Such natural structures are of high value as bio-templates for the development of bio-inorganic composites for many applications. In this study we used genetically modified filamentous Escherichia coli cells as template for the design of polyelectrolyte tubes that can be used as carrier for functional molecules or particles. Diversity of structures of biogenic materials has the potential to be used to construct inorganic or polymeric superior hybrid materials that reflect the form of the bio-template. Such bio-inspired materials are of great interest in diverse scientific fields like Biology, Chemistry and Material Science and can find application for the construction of functional materials or the bio-inspired synthesis of inorganic nanoparticles. RESULTS: Genetically modified filamentous E. coli cells were fixed in 2% glutaraldehyde and coated with alternating six layers of the polyanion polyelectrolyte poly(sodium-4styrenesulfonate) (PSS) and polycation polyelectrolyte poly(allylamine-hydrochloride) (PAH). Afterwards we dissolved the E. coli cells with 1.2% sodium hypochlorite, thus obtaining hollow polyelectrolyte tubes of 0.7 µm in diameter and 5-50 µm in length. For functionalisation the polyelectrolyte tubes were coated with S-layer protein polymers followed by metallisation with Pd(0) particles. These assemblies were analysed with light microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and transmission electron microscopy. CONCLUSION: The thus constructed new material offers possibilities for diverse applications like novel catalysts or metal nanowires for electrical devices. The novelty of this work is the use of filamentous E. coli templates and the use of S-layer proteins in a new material construct.
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Cápsulas Bacterianas/química , Cápsulas Bacterianas/metabolismo , Electrólitos/metabolismo , Escherichia coli/metabolismo , Microbiología Industrial/métodos , Cápsulas Bacterianas/genética , Cápsulas Bacterianas/ultraestructura , Electrólitos/química , Escherichia coli/química , Escherichia coli/genética , Escherichia coli/ultraestructura , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Nanopartículas/microbiologíaRESUMEN
The field of smooth vector graphics explores the representation, creation, rasterization, and automatic generation of light-weight image representations, frequently used for scalable image content. Over the past decades, several conceptual approaches on the representation of images with smooth gradients have emerged that each led to separate research threads, including the popular gradient meshes and diffusion curves. As the computational models matured, the mathematical descriptions diverged and papers started to focus more narrowly on subproblems, such as on the representation and creation of vector graphics, or the automatic vectorization from raster images. Most of the work concentrated on a specific mathematical model only. With this survey, we describe the established computational models in a consistent notation to spur further knowledge transfer, leveraging the recent advances in each field. We therefore categorize vector graphics papers from the last decades based on their underlying mathematical representations as well as on their contribution to the vector graphics content creation pipeline, comprising representation, creation, rasterization, and automatic image vectorization. This survey is meant as an entry point for both artists and researchers. We conclude this survey with an outlook on promising research directions and challenges to overcome in the future.
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The number of online news articles available nowadays is rapidly increasing. When exploring articles on online news portals, navigation is mostly limited to the most recent ones. The spatial context and the history of topics are not immediately accessible. To support readers in the exploration or research of articles in large datasets, we developed an interactive 3D globe visualization. We worked with datasets from multiple online news portals containing up to 45,000 articles. Using agglomerative hierarchical clustering, we represent the referenced locations of news articles on a globe with different levels of detail. We employ two interaction schemes for navigating the viewpoint on the visualization, including support for hand-held devices and desktop PCs, and provide search functionality and interactive filtering. Based on this framework, we explore additional modules for jointly exploring the spatial and temporal domain of the dataset and incorporating live news into the visualization.
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Corporate meetings are a crucial part of business activities. While numerous academic papers investigated how to make the scheduling process of meetings faster or even automatic, little work has been done yet to facilitate the retrospective reasoning about how time is spent on meetings. Traditional calendar applications do not allow users to extract actionable statistics although it has been shown that reflection-oriented design can increase the users' understanding of their habits and can thereby encourage a shift towards better practices. In this paper, we present MineTime Insight, a tool made of multiple coordinated views for the exploration of personal calendar data, with the overarching goal of improving short and long-term scheduling decisions. Despite being focused on the working environment, our work builds upon recent results in the field of Personal Visual Analytics, as it targets users not necessarily expert in visualization and data analysis. We demonstrate the potential of MineTime Insight, when applied to the agenda of an executive manager. Finally, we discuss the results of an informal user study and a field study. Our results suggest that our visual representations are perceived as easy to understand and helpful towards a change in the scheduling habits.
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In recent years, deep learning has opened countless research opportunities across many different disciplines. At present, visualization is mainly applied to explore and explain neural networks. Its counterpart-the application of deep learning to visualization problems-requires us to share data more openly in order to enable more scientists to engage in data-driven research. In this paper, we construct a large fluid flow data set and apply it to a deep learning problem in scientific visualization. Parameterized by the Reynolds number, the data set contains a wide spectrum of laminar and turbulent fluid flow regimes. The full data set was simulated on a high-performance compute cluster and contains 8000 time-dependent 2D vector fields, accumulating to more than 16 TB in size. Using our public fluid data set, we trained deep convolutional neural networks in order to set a benchmark for an improved post-hoc Lagrangian fluid flow analysis. In in-situ settings, flow maps are exported and interpolated in order to assess the transport characteristics of time-dependent fluids. Using deep learning, we improve the accuracy of flow map interpolations, allowing a more precise flow analysis at a reduced memory IO footprint.
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Aprendizaje Profundo , Gráficos por Computador , Aprendizaje Automático , Redes Neurales de la ComputaciónRESUMEN
Escherichia coli is one of the best studied micro-organisms and is the most widely used host in genetic engineering. The Gram-negative single cells are rod-shaped, and filaments are usually not found. Here, we describe the reproducible formation of elongated E. coli cells. During heterologous expression of the silent surface (S)-layer protein gene sllB from Lysinibacillus sphaericus JG-A12 in E. coli BL21(DE3), the cells were arranged as long chains which were surrounded by highly stable sheaths. These filaments had a length of >100 µm. In the stationary growth phase, microscopic analyses demonstrated the formation of unusually long transparent tube-like structures which were enclosing separate single cells. The tube-like structures were isolated and analysed by SDS-PAGE, infrared-spectroscopy and different microscopic methods in order to identify their unusual composition and structure. The tube-like structures were found to be like outer membranes, containing high levels of proteins and to which the recombinant S-layer proteins were attached. Despite the entire structure being indicative of a disordered cell division, the bacterial cells were highly viable and stable. To our knowledge, this is the first time that the induction of drastic morphological changes in E. coli by the expression of a foreign protein has been reported.
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Bacillaceae/genética , Proteínas de la Membrana Bacteriana Externa/genética , Escherichia coli/crecimiento & desarrollo , Expresión Génica , Glicoproteínas de Membrana/genética , Proteínas de la Membrana Bacteriana Externa/química , Proteínas de la Membrana Bacteriana Externa/metabolismo , Escherichia coli/química , Escherichia coli/genética , Escherichia coli/metabolismo , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/metabolismo , Estabilidad Proteica , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismoRESUMEN
Almost all properties of vector fields, including magnitude, direction, λ2 and vorticity change under arbitrary movements of the observer. This is undesirable since measurements of physical properties should ideally not depend on the way the (virtual) measurement device moves. There are some properties that are invariant under certain types of reference frame transformations: Galilean invariance (invariance under equal-speed translation) and objectivity (invariance under any smooth rotation and translation of the reference frame). In this paper, we introduce even harder conditions than objectivity: we demand invariance under any smooth similarity transformation (rotation, translation and uniform scale) as well as invariance under any smooth affine transformation of the reference frame. We show that these new hyper-objective measures allow the extraction of vortices that change their volume or deform. Further, we present a generic approach that transforms almost any vortex measure into a hyper-objective one. We apply our methods to vortex extraction in 2D and 3D vector fields, and analyze the numerical robustness, extraction time and the minimization residuals for the Galilean invariant, objective, and the two new hyper-objective approaches.
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The topological analysis of unsteady vector fields remains to this day one of the largest challenges in flow visualization. We build up on recent work on vortex extraction to define a time-dependent vector field topology for 2D and 3D flows. In our work, we split the vector field into two components: a vector field in which the flow becomes steady, and the remaining ambient flow that describes the motion of topological elements (such as sinks, sources and saddles) and feature curves (vortex corelines and bifurcation lines). To this end, we expand on recent local optimization approaches by modeling spatially-varying deformations through displacement transformations from continuum mechanics. We compare and discuss the relationships with existing local and integration-based topology extraction methods, showing for instance that separatrices seeded from saddles in the optimal frame align with the integration-based streakline vector field topology. In contrast to the streakline-based approach, our method gives a complete picture of the topology for every time slice, including the steps near the temporal domain boundaries. With our work it now becomes possible to extract topological information even when only few time slices are available. We demonstrate the method in several analytical and numerically-simulated flows and discuss practical aspects, limitations and opportunities for future work.
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In this article, we address three different topics in scientific visualization. The first part introduces optimization strategies that determine the visibility of line and surface geometry, such that a balance between occlusion avoidance and preservation of context is found. The second part proposes new methods for the visualization of time-dependent fluid flows, including the accurate depiction of Lagrangian scalar fields, as well as a new category of vortex identification methods. The third part introduces finite-sized particles as new application area for flow visualization, covering geometry-based methods, particle separation, topology, vortex corelines, and the determination of the origin of finite-sized particles.
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Time-dependent fluid flows often contain numerous hyperbolic Lagrangian coherent structures, which act as transport barriers that guide the advection. The finite-time Lyapunov exponent is a commonly-used approximation to locate these repelling or attracting structures. Especially on large numerical simulations, the FTLE ridges can become arbitrarily sharp and very complex. Thus, the discrete sampling onto a grid for a subsequent direct volume rendering is likely to miss sharp ridges in the visualization. For this reason, an unbiased Monte Carlo-based rendering approach was recently proposed that treats the FTLE field as participating medium with single scattering. This method constructs a ground truth rendering without discretization, but it is prohibitively slow with render times in the order of days or weeks for a single image. In this paper, we accelerate the rendering process significantly, which allows us to compute video sequence of high-resolution FTLE animations in a much more reasonable time frame. For this, we follow two orthogonal approaches to improve on the rendering process: the volumetric light path integration in gradient domain and an acceleration of the transmittance estimation. We analyze the convergence and performance of the proposed method and demonstrate the approach by rendering complex FTLE fields in several 3D vector fields.
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Over the past decades, scientific visualization became a fundamental aspect of modern scientific data analysis. Across all data-intensive research fields, ranging from structural biology to cosmology, data sizes increase rapidly. Dealing with the growing large-scale data is one of the top research challenges of this century. For the visual exploratory data analysis, interactivity, a view-dependent visibility optimization and frame coherence are indispensable. In this work, we extend the recent decoupled opacity optimization framework to enable a navigation without occlusion of important features through large geometric data. By expressing the accumulation of importance and optical depth in Fourier basis, the computation, evaluation and rendering of optimized transparent geometry become not only order-independent, but also operate within a fixed memory bound. We study the quality of our Fourier approximation in terms of accuracy, memory requirements and efficiency for both the opacity computation, as well as the order-independent compositing. We apply the method to different point, line and surface data sets originating from various research fields, including meteorology, health science, astrophysics and organic chemistry.
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Potential vorticity is among the most important scalar quantities in atmospheric dynamics. For instance, potential vorticity plays a key role in particularly strong wind peaks in extratropical cyclones and it is able to explain the occurrence of frontal rain bands. Potential vorticity combines the key quantities of atmospheric dynamics, namely rotation and stratification. Under suitable wind conditions elongated banners of potential vorticity appear in the lee of mountains. Their role in atmospheric dynamics has recently raised considerable interest in the meteorological community for instance due to their influence in aviation wind hazards and maritime transport. In order to support meteorologists and climatologists in the analysis of these structures, we developed an extraction algorithm and a visual exploration framework consisting of multiple linked views. For the extraction we apply a predictor-corrector algorithm that follows streamlines and realigns them with extremal lines of potential vorticity. Using the agglomerative hierarchical clustering algorithm, we group banners from different sources based on their proximity. To visually analyze the time-dependent banner geometry, we provide interactive overviews and enable the query for detail on demand, including the analysis of different time steps, potentially correlated scalar quantities, and the wind vector field. In particular, we study the relationship between relative humidity and the banners for their potential in indicating the development of precipitation. Working with our method, the collaborating meteorologists gained a deeper understanding of the three-dimensional processes, which may spur follow-up research in the future.