Pesticide impacts on avian species with special reference to farmland birds: a review.

For decades, we have observed a major biodiversity crisis impacting all taxa. Avian species have been particularly well monitored over the long term, documenting their declines. In particular, farmland birds are decreasing worldwide, but the contribution of pesticides to their decline remains controversial. Most studies addressing the effects of agrochemicals are limited to their assessment under controlled laboratory conditions, the determination of lethal dose 50 (LD50) values and testing in a few species, most belonging to Galliformes. They often ignore the high interspecies variability in sensitivity, delayed sublethal effects on the physiology, behaviour and life-history traits of individuals and their consequences at the population and community levels. Most importantly, they have entirely neglected to test for the multiple exposure pathways to which individuals are subjected in the field (cocktail effects). The present review aims to provide a comprehensive overview for ecologists, evolutionary ecologists and conservationists. We aimed to compile the literature on the effects of pesticides on bird physiology, behaviour and life-history traits, collecting evidence from model and wild species and from field and lab experiments to highlight the gaps that remain to be filled. We show how subtle nonlethal exposure might be pernicious, with major consequences for bird populations and communities. We finally propose several prospective guidelines for future studies that may be considered to meet urgent needs.

Comparison of pesticide contamination between captive-reared and wild grey partridges: insights into environmental exposure disparities.

Pesticide contamination is often cited as a key factor in the global decline of farmland birds. However, the majority of studies on pesticide exposure in non-target fauna are not representative of what happens in nature because they are limited to artificial conditions. The aim of this study was to define and compare, for the first time, pesticide contamination in grey partridges (Perdix perdix) from two different contexts, i.e., captivity vs. the wild. Blood samples taken from 35 captive and 54 wild partridges in 2021-2022 were analysed for 94 pesticides most commonly used in French agriculture. Captive partridges had 29 molecules detected in their blood (12 herbicides, 14 fungicides, and three insecticides) compared to wild partridges, which had 50 molecules (13 herbicides, 23 fungicides, and 14 insecticides). Of these pesticide compounds found in individuals, 26 were banned. Captive partridges had significantly fewer pesticide molecules than wild partridges, with one to 14 pesticides per captive individual and 8 to 20 pesticides per wild individual. Nineteen molecules were common to both groups, with concentrations up to three times higher in wild partridges than in captive partridges. Our results thus show multiple exposures for most of our individuals, especially in wild partridges, which can lead to cocktail effects, which are never considered. Furthermore, the difference in contamination between the wild and captive partridges reflects the multiple routes of contamination in nature, in particular, due to the use of a wide range of habitats by wild partridges.

Neonicotinoids: Still present in farmland birds despite their ban.

Neonicotinoids (neonics) are the most widely used insecticides worldwide and are considered to be of low risk to non-target organisms such as vertebrates. Further, they are reported to be rapidly excreted and metabolized, reducing their potential toxicity. Nevertheless, growing evidence of adverse effects of neonics on farmland bird species raise questions about the purported harmless nature of these pesticides. We attempted to search for pesticide residues in species of different trophic levels and at different life stages, by using multiple bird monitoring programs on a Long-Term Socio-Ecological Research (LTSER) platform. Three passerine birds-the blackbird (Turdus merula), cirl bunting (Emberiza cirlus), and common nightingale (Luscinia megarhynchos)-that feed on seeds and invertebrates were monitored during their reproductive period, and the grey partridge (Perdix perdix) that feeds on seeds was monitored during its wintering period. We also monitored chicks of an apex predator-the Montagu's harrier (Circus pygargus)-that preys mostly upon common voles but also upon insects. We found that the birds' blood samples showed presence of residues of five neonics: three banned since 2018 in France-clothianidin, thiacloprid, and thiamethoxam-and two-dinotefuran and nitenpyram-used for veterinary purposes only. While none of these neonics was detected in blackbirds, all were present in grey partridges. Clothianidin was detected in all species, except blackbirds. Concentrations of the three banned neonics were similar or higher than concentrations found in birds monitored elsewhere before the ban. These findings raise questions about the persistence of neonics within the environment and the mode of exposure to wild fauna. Future investigations on the sublethal effects of these neonics on life-history traits of these farmland birds may help in providing a better understanding of the effects of exposure of bird populations to these insecticides, and also to the consequent effect on human health.

Feeding on grains containing pesticide residues is detrimental to offspring development through parental effects in grey partridge.

Numerous toxicological studies have shown that ingestion of pesticides can induce physiological stress in breeding birds, with adverse consequences on egg laying parameters and offspring quality through parental effects. However, previous studies do not mimic current levels of pesticide residues in typical landscapes, and they do not consider potential cocktail effects of pesticides as they occur in the wild. Herein, we explored whether realistic pesticide exposure affected reproduction parameters and offspring condition through parental effects in Grey partridge. We fed 24 breeding pairs with either seeds from conventional agriculture crops treated with various pesticides during cropping, or organic grains without pesticide residues as controls. The conventional and organic grain diets mimicked food options potentially encountered by wild birds in the field. The results showed that ingesting low pesticide doses over a long period had consequences on reproduction and offspring quality without altering mortality in parents or chicks. Compared with organic pairs, conventional pairs yielded smaller chicks at hatching that had a lower body mass index at 24 days old. Additionally, these chicks displayed lower haematocrit when body mass index was higher. Therefore, ingestion of conventional grains by parents resulted in chronic exposure to pesticide residues, even at low doses, and this had detrimental consequences on offspring. These results demonstrate a sublethal effect of pesticide residues through parental effects. The consequences of parental exposure on chicks might partly explain the decline in wild Grey partridge populations, which raises questions for avian conservation and demography if current agrosystem approaches are continued.