RESUMEN
BACKGROUND AND PURPOSE: The carotid stent placement as a therapeutic option for carotid stenosis has been increasing among years; therefore, studies are required to evaluate the security and efficacy of its materials. The purpose of this study was to evaluate the distal filter and the proximal balloon-guided catheter with flow inversion as protection devices during carotid angioplasty and stenting. METHODS: This is a retrospective, observational study of patients diagnosed with carotid stenosis treated with angioplasty between January 1, 2014, and June 30, 2020; we analyzed a radiology service database to compare the distal filter and the proximal balloon-guided catheter as protection devices during angioplasty. RESULTS: One hundred seventy-five angioplasties were performed, the distal filter was the most prevalent embolic protection device used (66%), patients baseline characteristics did not differ between groups with different embolic protection devices, except for history of dyslipidemia (p < 0.000). As well, we did not find any significant differences between the groups in the device related complications, intervention time (p = 0.140), unrelated complications (p = 0.693) and functional independence at 90 days (p = 0.096). CONCLUSIONS: In our study the proximal balloon-guided catheter and the distal filter protection device as protection devices during the carotid stenting didn't show significant differences regarding complications related to the system.
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Angioplastia de Balón , Estenosis Carotídea , Humanos , Estenosis Carotídea/diagnóstico por imagen , Estenosis Carotídea/cirugía , Colombia , Resultado del Tratamiento , Catéteres , StentsRESUMEN
INTRODUCTION: A third of hypertensive patients have major depression, a relationship that is associated with a worse prognosis. The objective of the study was to estimate the association between depression and high blood pressure, as well as to establish the possible bidirectionality of the conditions. MATERIALS AND METHODS: Retrospective cohort study. People between 18 and 65 years old with high blood pressure, depression or use of medications for their management were included. To analyze the antecedent, a comorbidity model was performed. A bivariate analysis was performed and then a multivariate logistic regression. The association was estimated using the Chi-square test and the odds ratios that were crude and adjusted to the other variables included in the analysis. The Hosmer-Lemeshow test was used to assess the goodness of fit. SPSS® v.21 was used as the statistical package. RESULTS: A total of 1,721 people were included in the study. The prevalence of depression in patients with and without hypertension was 17.4 and 12.6%, respectively, with a 43% risk of hypertension in people with depression. In patients with depression, it preceded the diagnosis of hypertension in 64.8% of cases and in hypertensive patients, 22.2% were later diagnosed with depression. The association between high blood pressure and major depression remained significant after adjusting for the other risk factors. CONCLUSIONS: Depression was found as a risk factor for high blood pressure, with a 2-way risk relationship between depression and high blood pressure.
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Trastorno Depresivo Mayor/epidemiología , Hipertensión/epidemiología , Adolescente , Adulto , Anciano , Estudios de Cohortes , Colombia , Trastorno Depresivo Mayor/complicaciones , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores de Riesgo , Adulto JovenRESUMEN
Here we report the cloning of a cDNA encoding the first nucleoside diphosphate kinase (NDPK) isolated from plant mitochondria. Amplification of a 317 nt product was performed by PCR, using oligonucleotide primers based on partial amino acid sequences of the pea mitochondria NDPK and other NDPK isoforms. By screening of a pea cDNA library with this PCR product, a full length clone was obtained. Northern analysis revealed the presence of a 1.1 kb single transcript, with high expression in young leaves and reproductive tissues. The clone encodes a precursor protein of 232 amino acids (26 kDa), including an N-terminal extension of 80 amino acids (9 kDa). Analysis of the deduced amino acid sequence confirmed its identity with the sequences obtained from the purified mature pea mitochondrial NDPK. In vitro import experiments carried out in isolated pea mitochondria showed targeting and processing of the 27 kDa precursor into a 16.5 kDa mature form. Phylogenetic analysis of some vertebrate and plant isoforms of NDPK showed that the pea mtNDPK groups together with the NDPK3 isoform from A. thaliana and the chloroplastic NDPK III from spinach. We suggest that it is possible to design a novel classification of the different NDPK isoforms according to their subcellular localisation and origin.
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Mitocondrias/enzimología , Nucleósido-Difosfato Quinasa/genética , Pisum sativum/enzimología , Pisum sativum/genética , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Cartilla de ADN/genética , ADN Complementario/genética , ADN Complementario/aislamiento & purificación , ADN de Plantas/genética , ADN de Plantas/aislamiento & purificación , Expresión Génica , Datos de Secuencia Molecular , Filogenia , Homología de Secuencia de AminoácidoRESUMEN
In isolated pea (Pisum sativum L.) mitochondria incorporation of 35S-methionine into newly synthesised proteins was influenced by the presence of site-specific inhibitors of the respiratory electron-transport chain. These effects were not produced by changes in the rate of respiratory electron transport itself nor by changes in ATP concentration. Protein synthesis was inhibited by inhibitors of ubiquinone reduction but not by inhibitors of ubiquinol oxidation. By the use of additional inhibitors at specific sites of the respiratory chain, different oxidation-reduction states were obtained for the different complexes in the electron-transport chain. It was found that electron transport through succinate:ubiquinone oxidoreductase (respiratory complex II) was specifically required for protein synthesis, even when all the other conditions for protein synthesis were satisfied. We suggest that a subunit of complex II, or a component closely associated with complex II, is involved in a regulatory system that couples electron transport to protein synthesis.
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Mitocondrias/metabolismo , Complejos Multienzimáticos/metabolismo , Oxidorreductasas/metabolismo , Pisum sativum/metabolismo , Proteínas de Plantas/biosíntesis , Biosíntesis de Proteínas , Succinato Deshidrogenasa/metabolismo , Ciclo del Ácido Cítrico , Complejo II de Transporte de Electrones , Regulación Fúngica de la Expresión Génica , Consumo de OxígenoRESUMEN
In this work we have further characterized the first mitochondrial nucleoside diphosphate kinase (mtNDPK) isolated from plants. The mitochondrial isoform was found to be especially abundant in reproductive and young tissues. Expression of the pea (Pisum sativum L. cv Oregon sugarpod) mtNDPK was not affected by different stress conditions. However, the pea mtNDPK was found to interact with a novel 86-kD protein, which is de novo synthesized in pea leaves upon exposure to heat. Thus, we have evidence for the involvement of mtNDPK in mitochondrial heat response in pea in vivo. Studies on oligomerization revealed that mtNDPK was found in complexes of various sizes, corresponding to the sizes of e.g. hexamers, tetramers, and dimers, indicating flexibility in oligomerization. This flexibility, also found for other NDPK isoforms, has been correlated with the ability of this enzyme to interact with other proteins. We believe that the mtNDPK is involved in heat stress response in pea, possibly as a modulator of the 86-kD protein.
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Respuesta al Choque Térmico , Mitocondrias/enzimología , Nucleósido-Difosfato Quinasa/metabolismo , Pisum sativum/fisiología , Proteínas de Plantas/metabolismo , Biopolímeros , Western Blotting , Inmunohistoquímica , Pisum sativum/enzimología , Pisum sativum/metabolismo , Pruebas de PrecipitinaRESUMEN
Polymorphisms in CC chemokine receptor 5 (CCR5), the major coreceptor of human immunodeficiency virus 1 (HIV-1) and simian immunodeficiency virus (SIV), have a major influence on HIV-1 transmission and disease progression. The effects of these polymorphisms may, in part, account for the differential pathogenesis of HIV-1 (immunosuppression) and SIV (natural resistance) in humans and non-human primates, respectively. Thus, understanding the genetic basis underlying species-specific responses to HIV-1 and SIV could reveal new anti-HIV-1 therapeutic strategies for humans. To this end, we compared CCR5 structure/evolution and regulation among humans, apes, Old World Monkeys, and New World Monkeys. The evolution of the CCR5 cis-regulatory region versus the open reading frame as well as among different domains of the open reading frame differed from one another. CCR5 cis-regulatory region sequence variation in humans was substantially higher than anticipated. Based on this variation, CCR5 haplotypes could be organized into seven evolutionarily distinct human haplogroups (HH) that we designated HHA, -B, -C, -D, -E, -F, and -G. HHA haplotypes were defined as ancestral to all other haplotypes by comparison to the CCR5 haplotypes of non-human primates. Different human and non-human primate CCR5 haplotypes were associated with differential transcriptional regulation, and various polymorphisms resulted in modified DNA-nuclear protein interactions, including altered binding of members of the NF-kappaB family of transcription factors. We identified novel CCR5 untranslated mRNA sequences that were conserved in human and non-human primates. In some primates, mutations at exon-intron boundaries caused loss of expression of selected CCR5 mRNA isoforms or production of novel mRNA isoforms. Collectively, these findings suggest that the response to HIV-1 and SIV infection in primates may have been driven, in part, by evolution of the elements controlling CCR5 transcription and translation.
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Evolución Molecular , VIH-1/genética , ARN Mensajero/genética , Receptores CCR5/genética , Virus de la Inmunodeficiencia de los Simios/genética , Factores de Transcripción/metabolismo , Transcripción Genética , Animales , Secuencia de Bases , VIH-1/patogenicidad , Haplotipos , Humanos , Datos de Secuencia Molecular , Polimorfismo Genético , Primates/genética , Unión Proteica , Empalme del ARN , Secuencias Reguladoras de Ácidos Nucleicos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Ácido Nucleico , Virus de la Inmunodeficiencia de los Simios/patogenicidadRESUMEN
Entre los años 1995 y 2003, fueron reimplantados un total de 490 unidades renales por técnica extravesical según Lich-Gregoir. Todos los casos correspondieron a pacientes pediátricos de ambos sexos portadores de reflujo vesico renal primario. Del total de unidades renales tratadas. 2 por ciento experimentaron persistencia del reflujo. El éxito global en el tratamiento de reflujo se logró en 480 unidades renales (98 por ciento). El porcentaje de hematuria macroscópica (4.08 por ciento) y disuria (2.71 por ciento) fue significativamente menor que el observado en las técnicas transvesicales (100 por ciento). El tiempo de estancia hospitalaria fue menos de 24 horas en 58 por ciento de los casos; entre 24 y 48 horas en 26 por ciento de los mismos, y sólo 16 por ciento de los infantes necesitaron 2 o mas días de hospitalización. Considerando los resultados, recomendamos la utilización de la técnica extravesical de Lich-Gregoir para el tratamiento del reflujo vesico renal primario de II, III y IV grado ya que ofrece menor morbilidad que las técnicas transvesicales.
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Humanos , Masculino , Femenino , Niño , Cateterismo Urinario , Infecciones Urinarias/diagnóstico , Infecciones Urinarias/terapia , Reflujo Vesicoureteral/diagnóstico , Reflujo Vesicoureteral/patología , Reflujo Vesicoureteral/terapia , Terapia de Reemplazo Renal/métodos , Trasplante de Riñón/métodos , Uréter/anomalías , Disuria/complicaciones , Disuria/diagnóstico , Hematuria/complicaciones , Hematuria/diagnóstico , PediatríaRESUMEN
Descripción de la técnica quirúrgica y evaluación en nuestra casuística del tratamiento de la incontinencia urinaria de esfuerzo con cinta vaginal libre de tensión (TVT). Se realizo una revisión de la literatura acerca de este nuevo método. Se revisaron las historias de las pacientes sometidas a este procedimiento en el Hospital Patrocinio Peñuela Ruíz, recolectando datos en tablas para la evaluación y análisis de la misma. Se encontraron un total de 14 casos, con un promedio de edad de 50 años. Se presentaron (2) casos de perforación de vejiga (14,2 por ciento), dos (2) casos presentaron retención urinaria en el postoperatorio inmediato (14,2 por ciento). La tasa de curación subjetiva fue de 81,8 por ciento. La mayoría de los casos fueron intervenidos en forma ambulatoria. Los resultados obtenidos son equiparables con los publicados en otras series. Concluimos se trata de una técnica sencilla, de fácil aprendizaje, con tiempo quirúrgico corto y corta estancia postoperatoria, siendo así una buena elección en el tratamiento de la incontinencia urinaria de esfuerzo.
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Humanos , Femenino , Anestesia Local/métodos , Incontinencia Urinaria de Esfuerzo/cirugía , Incontinencia Urinaria de Esfuerzo/diagnóstico , Incontinencia Urinaria de Esfuerzo/patología , Mallas Quirúrgicas , Vejiga Urinaria/cirugía , Polipropilenos , Procedimientos Quirúrgicos Urogenitales/métodosRESUMEN
Las fases hispatológicas de la Apendicitis Aguda se usan para la descripción de los hallazgos intraoperatorios por parte de los cirujanos. Siendo la fase en que se encuentra el apéndice importante para escoger el tratamiento subsecuente, actualmente no contamos con el estudio histopatológico en postoperatorio inmediato. Quisimos verificar si los hallazgos intraoperatorios coinciden con los histopatológicos. Se revisaron 160 casos con Diagnóstico de Apendicitis Aguda, en los años de 1999 y 2000, en el Hospital "Dr. Patrocinio Peñuela Ruíz. Se agrupo los hallazgos intraoperatorios e histopatológicos en las cuatro fases clásicas de la Apendicitis, luego se compararon los grupos por cada una de las fases. Se usó el Test de Fisher. Donde hubo mayor coincidencia fue en el grupo de apéndices sin alteraciones. En el resto se evidencia una tendencia similar a subclasificar el estado de la Apéndice macroscópicamente lo cual hace al método poco viable para establecer tratamientos.
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Humanos , Masculino , Femenino , Apendicitis/cirugía , Apendicitis/diagnóstico , Histología , Inflamación/complicaciones , Cuidados Posoperatorios/normas , Venezuela/epidemiologíaRESUMEN
Se estudiaron 149 casos de pacientes operados con diagnóstico histológico de apendicitis aguda, en el HPPR durante el período 1999-2000. El objetivo del mismo fue determinar la existencia o no de una correlación entre las horas de evolución y el diagnóstico histopatológico. Se recolectaron los siguientes datos de las historias clínicas: horas de evolución del dolor abdominal y diagnóstico histológico. Se clasificaron los pacientes en cuatro grupos según el tiempo de evolución del dolor (12 horas o menos, 12 a 24 horas, 24 a 48 horas). La mayor parte de los pacientes (47 por ciento) consultaron entre las 24 y 48 horas de iniciado el dolor. Para los cuatro grupos el hallazgo histológico más frecuente fue apendicitis flegmonosa y hemorrágica (46,51 por ciento, 65,51 por ciento, 55,32 por ciento y 40 por ciento respectivamente). Se concluye que no hay relación directa entre las horas de evolución del dolor y la fase histológica.