RESUMEN
Baicalin is one bioactive flavone with anti-inflammatory and neuroprotective activities. The neuroprotective effects of baicalin on pathological changes and behavioral deficits were explored in a mouse model of amyloid ß (Aß)(1-42) protein-induced Alzheimer's disease (AD). Mice received a bilateral injection of Aß(1-42) protein into the hippocampus, then they were treated with baicalin (30, 50 and 100 mg/kg body weight, orally) or Tween 80. The therapeutic effects of baicalin were monitored by Morris water maze trial and probe test. Then mice were sacrificed for immunohistochemistry and western blot analysis. After a relatively short-term treatment of 14 days, 100 mg/kg of baicalin significantly ameliorated memory impairment in the Morris water maze test and probe test, and also attenuated glial cell activations and increase of TNF-α and IL-6 expressions induced by Aß(1-42) protein. These results suggest that baicalin ameliorated Aß(1-42) protein-related pathology and cognitive dysfunction via its anti-neuroinflammatory activity, and may be a potential candidate for the treatment of AD.
Asunto(s)
Enfermedad de Alzheimer/tratamiento farmacológico , Enfermedad de Alzheimer/psicología , Péptidos beta-Amiloides/toxicidad , Antiinflamatorios no Esteroideos/uso terapéutico , Flavonoides/uso terapéutico , Trastornos de la Memoria/tratamiento farmacológico , Trastornos de la Memoria/etiología , Fragmentos de Péptidos/toxicidad , Enfermedad de Alzheimer/inducido químicamente , Animales , Trastornos del Conocimiento/tratamiento farmacológico , Trastornos del Conocimiento/psicología , Citocinas/metabolismo , Inmunohistoquímica , Activación de Macrófagos/efectos de los fármacos , Masculino , Aprendizaje por Laberinto/efectos de los fármacos , Trastornos de la Memoria/inducido químicamente , Ratones , Ratones Endogámicos ICR , Neuroglía/efectos de los fármacosRESUMEN
OBJECTIVE: To study the effects of podophyllic acid on proliferation and apoptosis in human leukemia K562 cells. METHODS: The inhibitory effects of podophyllic acid on K562 cells were investigated by clony-formation and MTT colorimetric assay. The apoptotic effects were observed by FCM. RESULTS: Podophylic acid could inhibit the proliferation of K562 cells and there were a significant difference between experimental and control groups (P < 0.01). There was a marked positive correlation between drug concentration and the inhibitory rates. Podophyllic acid could induce apoptosis of K562 cells. When K562 cells were treated with 5.0 microg/ml podophyllic acid for 48 hours, apoptosis occurred to the largest extent. The apoptosis rate of podophyllic acid was 33.5%. CONCLUSIONS: Podophyllic acid has antitumor effect, but the change of structure affects the function of derivatives of podophylloxin greatly.