Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition.

In both plants and animals, nucleotide-binding leucine-rich repeat (NLR) immune receptors play critical roles in pathogen recognition and activation of innate immunity. In plants, NLRs recognise pathogen-derived effector proteins and initiate effector-triggered immunity (ETI). However, the molecular mechanisms that link NLR-mediated effector recognition and downstream signalling are not fully understood. By exploiting the well-characterised tomato Prf/Pto NLR resistance complex, we identified the 14-3-3 proteins TFT1 and TFT3 as interacting partners of both the NLR complex and the protein kinase MAPKKKα. Moreover, we identified the helper NRC proteins (NLR-required for cell death) as integral components of the Prf /Pto NLR recognition complex. Notably our studies revealed that TFTs and NRCs interact with distinct modules of the NLR complex and, following effector recognition, dissociate facilitating downstream signalling. Thus, our data provide a mechanistic link between activation of immune receptors and initiation of downstream signalling cascades.

Phloem: At the center of action in plant defense against aphids.

The location of the phloem deep inside the plant, the high hydrostatic pressure in the phloem, and the composition of phloem sap, which is rich in sugar with a high C:N ratio, allows phloem sap feeding insects to occupy a unique ecological niche. The anatomy and physiology of aphids, a large group of phytophagous insects that use their mouthparts, which are modified into stylets, to consume large amounts of phloem sap, has allowed aphids to successfully exploit this niche, however, to the detriment of agriculture and horticulture. The ability to reproduce asexually, a short generation time, the development of resistance to commonly used insecticides, and their ability to vector viral diseases makes aphids among the most damaging pests of plants. Here we review how plants utilize their ability to occlude sieve elements and accumulate antibiotic and antinutritive factors in the phloem sap to limit aphid infestation. In addition, we summarize progress on understanding how plants perceive aphids to activate defenses in the phloem.