Evaluation of Bond Strength and Fracture Load of Teeth With Flared Root Canals Restored With Prefabricated, Anatomical, and Computer-aided Design and Computer-aided Manufacturing Fiber Posts.

PURPOSE: This study compared the use of anatomical glass fiber posts using bulk-fill composite resin with computer-aided design and computer-aided manufacturing (CAD/CAM) milled glass fiber post in oversized root canals, through bond strength (BS) and fracture resistance (FR) tests (fracture load). METHODS AND MATERIALS: A total of 48 mandibular premolars were selected, half of them had their crowns removed at the cemento-enamel junction (CEJ) and the other half were sectioned 2 mm above the CEJ. Subsequently, teeth were endodontically treated. After 1 week, the standard preparation of the canals was carried out, and the roots were divided into three groups (n=16), according to the use of different restorative techniques (control: prefabricated glass fiber post [PFP], direct anatomical glass fiber post [AFP], and CAD/CAM milled glass fiber post [MFP]). After luting procedures using Single Bond Universal and RelyX Ultimate (3M ESPE), for eight teeth in each group, six specimens were obtained (two slices from each root third: cervical, middle, and apical). For the remaining eight roots of each group, standardized preparations for metal-free crowns, milling of 5 mol% yttria-stabilized tetragonal zirconia polycrystalline, cementation of the crowns, and periodontal ligament simulation were performed. Then, for each group, the BS was evaluated through the push-out test, and the FR was evaluated in compression. The data obtained from BS were submitted to two-way analysis of variance (ANOVA; group vs root region) and Tukey (α=0.05) and from FR to one-way ANOVA (group) and Tukey (α=0.05). RESULTS: For the BS test, the MFP group was statistically superior to the PFP group in all root regions and was statistically superior to the AFP group only in the cervical region, being statistically similar in the middle and apical root regions. For the FR test, the MFP group was statistically superior to the PFP and AFP groups. CONCLUSION: The milled fiber post technique can be a legitimate alternative in the restoration of weakened roots with flared root canals.

Advanced design of a x-ray absorption spectroscopy setup for measuring transition metals speciation in molten carbonates, hydroxides and hydrogenosulfates.

Battery recycling is currently becoming a crucial issue. One possible treatment path involves the use of molten salts. A mechanistic understanding of the underlying processes requires being able to analyze in situ speciation in molten salts at various temperatures. This can be advantageously achieved using x-ray absorption spectroscopy, the use of Quick-EXAFS facilities being particularly appropriate. Consequently, this paper presents the design and development of a new setup allowing carrying out Quick-EXAFS experiments in oxidizing molten salts at high temperatures. We describe the different components of a cell and the performance of the heating device. We illustrate the capabilities of the setup by analyzing the temperature evolution of Co speciation upon dissolution of LiCoO2, a typical battery electrode material, in molten carbonates, hydroxides, and hydrogenosulphates.

Impact of pH and application time of meta-phosphoric acid on resin-enamel and resin-dentin bonding.

PURPOSE: To evaluate the immediate microshear resin-enamel bond strength (µSBS) and the immediate and 6-month microtensile bond strength (µTBS) and nanoleakage (NL) of the adhesive interface performed by different pHs of 40% meta-phosphoric acid (MPA) were compared with conventional 37% ortho-phosphoric acid (OPA) under different application times. Additionally, the enamel etching patterns were evaluated and the chemical/morphological changes induced by these differents groups were evaluated. MATERIALS AND METHODS: One hundred and ninety-eight extracted human molars were randomly assigned into experimental groups according to the combination of independent variables: Acid [37% ortho-phosphoric acid (OPA), 40% meta-phosphoric acid (MPA) at pHs of: 0.5, 1 and 2] and Application Time [7, 15 and 30s]. Enamel-bond specimens were prepared and tested under µSBS. Resin-dentin beams were tested under µTBS tested immediately or after 6-months of water storage. Nanoleakage was evaluated using bonded-beams of each tooth/time-period. Enamel etching pattern and chemical and ultra-morphology analyses were also performed. The µSBS (MPa) data were subjected to a two-way repeated measures ANOVA (Acid vs. Application time). For µTBS, Acid vs application time vs storage time data were subjected to three-way ANOVA and Tukey's test (α = 0.05). RESULTS: MPA pH 0.5 showed µTBS similar to OPA, independently of the application time on enamel (p>0.05) or dentin (p>0.05). OPA provided higher nanoleakage values than MPA (p = 0.003). Significant decreases in TBS and increases in NL were only observed for OPA after 6 months (p = 0.001). An increase in the application time resulted in a more pronounced etching pattern for MPA. Chemical analysis showed that dentin demineralized by MPA depicted peaks of brushite and octacalcium phosphate. MPA exposed less collagen than OPA. However, optimal results for MPA were dependent on pH/application time. CONCLUSION: The use of 40% meta-phosphoric acid with a pH of 0.5 is an alternative acid-etching agent for dentin and enamel bonding. Furthermore, the use of MPA preserves the resin-dentin interface over a 6-months period, due to presence of brushite and octacalcium phosphate and a reduced demineralization pattern.

Three-Year Effects of Deproteinization on the In Vitro Durability of Resin/Dentin-Eroded Interfaces.

OBJECTIVE: To evaluate the effect of sodium hypochlorite on the immediate and three-year bonding properties of a resin-eroded dentin interface produced by one of two adhesive strategies. METHODS AND MATERIALS: Forty-eight molars were randomly assigned to six experimental groups, according to the combination of the adhesive strategy (etch-and-rinse and self-etch) and the dentin surface (control groups without erosion, eroded dentin surface [ED], and eroded dentin surface + NaOCl 5.2% [ED + NaOCl]). After completing restoration, specimens were stored in water (37°C) for 24 hours and then sectioned into resin-dentin beams (0.8 mm2) to be tested under tension (0.5 mm/min) immediately thereafter or after three years of water storage. To assess nanoleakage (NL), specimens were immersed in silver nitrate solution and examined by scanning electron microscopy at both time points. The dentin-etching pattern was examined under a scanning electron microscope. Data were subjected to appropriate statistical analysis (α=0.05) Results: In both strategies, a more pronounced and significant reduction of the microtensile bond strength (µTBS) values was observed for the ED groups ( p=0.0001) after three years. However, in the ED + NaOCl group, µTBS values were maintained after three years of water storage. Furthermore, application of NaOCl to eroded dentin significantly reduced the immediate NL values and also preserved these values after three years of water storage for both adhesive strategies ( p>0.05). When considering the ED group, a superficial removal of the smear layer and enlarged lumen tubules in comparison to control were present. However, for ED + NaOCl, there was a total removal of the smear layer and significant numbers of collagen fibrils were exposed. CONCLUSION: The use of NaOCl may maintain the long-term stability of a resin-eroded dentin interface formed by etch-and-rinse and self-etch adhesives.

Using CAD/CAM-Modified Correlation Mode to Produce Laminate Veneers: A Six-Month Case Report.

The expectation of an esthetically harmonious smile increases the level of difficulty when treating patients. Laminate veneers stand out as a treatment option for cosmetic rehabilitation in clinical practice, as they are a more conservative procedure and mimic dental structures. These laminate veneers are generally made with different techniques; the most common requires an impression of the prepared tooth, an impression antagonist, fabrication models, and extensive laboratory time. The computer-aided design/computer-aided manufacturing (CAD/CAM) system optimizes the fabrication of prosthetic structures, reducing chairside time and promoting good esthetic results. Thus, the purpose of this case report is to present the esthetic result of multiple CAD/CAM manufactured laminate veneers using a new self-etching glass ceramic primer with a lithium disilicate ceramic, using the modified correlation and biogeneric modes.

Four-year Follow-up of a Direct Anatomical Fiber Post and Esthetic Procedures: A Case Report.

Knowledge about the stability of fiber posts cemented in widened canal spaces over time is scarce in the literature. Thus, the purpose of this case report was to evaluate the performance of a direct anatomical post in a widened canal space over the course of four years. The present clinical case describes the rehabilitation of a widened canal space using a direct anatomical post (a resin composite combined with a prefabricated glass fiber post) associated with an all-ceramic crown and other restorative procedures. This technique is easy to perform and may solve some of the problems associated with the cementation of a poorly adapted fiber post in a widened canal space.

Use of a Direct Anatomic Post in a Flared Root Canal: A Three-year Follow-up.

The following case report describes the three-year follow-up after rehabilitation of a flared root canal using a direct anatomic post (a resin composite combined with a prefabricated glass fiber post) associated with metal-free ceramic restoration. The report presents the clinical protocol for the fabrication of the posts, which provide an intimate fit to the remaining root and mechanical properties similar to those of the dental structure. These posts serve as an alternative to conventional metal cores.

Differential effect of plant lectins on mast cells of different origins.

Histamine release induced by plant lectins was studied with emphasis on the carbohydrate specificity, external calcium requirement, metal binding sites, and mast cell heterogeneity and on the importance of antibodies bound to the mast cell membrane to the lectin effect. Peritoneal mast cells were obtained by direct lavage of the rat peritoneal cavity and guinea pig intestine and hamster cheek pouch mast cells were obtained by dispersion with collagenase type IA. Histamine release was induced with concanavalin A (Con A), lectins from Canavalia brasiliensis, mannose-specific Cymbosema roseum, Maackia amurensis, Parkia platycephala, Triticum vulgaris (WGA), and demetallized Con A and C. brasiliensis, using 1-300 microg/ml lectin concentrations applied to Wistar rat peritoneal mast cells, peaking on 26.9, 21.0, 29.1, 24.9, 17.2, 10.7, 19.9, and 41.5%, respectively. This effect was inhibited in the absence of extracellular calcium. The lectins were also active on hamster cheek pouch mast cells (except demetallized Con A) and on Rowett nude rat (animal free of immunoglobulins) peritoneal mast cells (except for mannose-specific C. roseum, P. platycephala and WGA). No effect was observed in guinea pig intestine mast cells. Glucose-saturated Con A and C. brasiliensis also released histamine from Wistar rat peritoneal mast cells. These results suggest that histamine release induced by lectins is influenced by the heterogeneity of mast cells and depends on extracellular calcium. The results also suggest that this histamine release might occur by alternative mechanisms, because the usual mechanism of lectins is related to their binding properties to metals from which depend the binding to sugars, which would be their sites to bind to immunoglobulins. In the present study, we show that the histamine release by lectins was also induced by demetallized lectins and by sugar-saturated lectins (which would avoid their binding to other sugars). Additionally, the lectins also released histamine from Rowett nude mast cells that are free of immunoglobulins.

Relationship of abomasal histology and parasite-specific immunoglobulin A with the resistance to Haemonchus contortus infection in three breeds of sheep.

This study was carried out to evaluate the relationship of abomasal inflammatory cells and parasite-specific immunoglobulin A (IgA) in mucus, with the resistance to Haemonchus contortus infection in three breeds of sheep naturally infected with gastrointestinal nematodes. The breeds were the native Santa Ines sheep, and the European Suffolk and Ile de France breeds. Mast cells, eosinophils and globule leucocytes were enumerated in abomasal mucosa. Eosinophils within the sub-mucosa also were counted separately. Histamine concentration was estimated in abomasal tissue samples. Enzyme-linked immunosorbent assay was carried out in mucus samples to determine the level of IgA anti-H. contortus third and fifth instar. There were no significant differences among group means of these variables (P>0.05). The correlation coefficients between fecal egg counts (FEC)xmast cells (r=-0.490; P<0.05) and FECxeosinophils in sub-mucosa (r=-0.714; P<0.01) was significant in the Santa Ines sheep. In the Ile de France group, the correlation coefficients between globule leucocytesxFEC (r=-0.879; P<0.001) and histaminexworm burden (r=-0.833; P<0.01) were also significant. In the Santa Ines and Ile de France sheep, correlation coefficients between IgA anti-L3xworm burden and IgA anti-L3xFEC were negative. In general, inflammatory cells and IgA-parasite-specific in abomasum were inversely associated with H. contortus worm burden and FEC indicating that they may impair parasite development or fecundity in the three breeds of sheep. However, similar mean values of inflammatory cells and IgA were found in the resistant (Santa Ines) and in the susceptible (Suffolk and Ile de France) breeds of sheep. The enumeration of cells by histological assessment does not provide information on their functional activity, which may be different among breeds. Thus, the effect of breed on the functional activity of these and other inflammatory cells is an important area for further study.

Impact of BAL in the management of pneumonia with treatment failure: positivity of BAL culture under antibiotic therapy.

BACKGROUND: Pneumonia is responsible for 50% of antibiotics prescribed in ICUs. Treatment failure, ie, absence of improvement or clinical deterioration under antibiotic therapy, presents a dilemma to physicians. BAL is an invasive method validated for etiologic diagnosis in pneumonia. STUDY OBJECTIVE: To evaluate in ICU patients the impact of BAL in the etiologic diagnosis, treatment, and outcome of pneumonia with treatment failure. DESIGN: Prospective clinical study. SETTING: Nonsurgical, medical ICU of a university hospital in Brazil. PATIENTS AND PARTICIPANTS: Sixty-two episodes of pneumonia treated for at least 72 h without clinical improvement in 53 patients hospitalized for diverse clinical emergencies. Mean duration of hospitalization was 14.2 days. Mean duration of previous antibiotic therapy was 11.4 days. INTERVENTIONS: Bronchoscopy and BAL were performed in each episode. BAL fluid was cultivated for aerobic and anaerobic bacteria; the cutoff considered positive was 10(4) cfu/mL; 10(3) cfu/mL was also analyzed if under treatment. Pneumocystis carinii, fungi, Legionella spp, and Mycobacterium spp were also researched. MEASUREMENTS AND RESULTS: Fifty-eight of 62 BAL were performed under antibiotics. The results showed positivity in 45 of 62 (72.6%); 42 of the 45 positive episodes (93.3%) had > 10(4) cfu/mL. The three cases with between 10(3) and 10(4) cfu/mL were considered positive and were treated according to BAL cultures. The main agents were Acinetobacter baumannii (37.1%), Pseudomonas aeruginosa (17.7%), and methicillin-resistant Staphylococcus aureus (MRSA; 16.1%); 46.7% of the episodes (21 of 45) were polymicrobial. BAL results directed a change of therapy in 34 episodes (54.8%). Overall mortality was 43.5%. There was no difference in mortality among positives, negatives, and patients who changed therapy guided by BAL culture. CONCLUSIONS: (1) BAL fluid examination was positive in 45 of 62 episodes (72.6%), with 58 of 62 BAL performed under antibiotics. This suggests that BAL may be a sensitive diagnostic method for treatment failures of clinically diagnosed pneumonias, even if performed under antibiotics; (2) the main pathogens in our study were A baumannii, P aeruginosa, and MRSA, and approximately 45% of infections were polymicrobial; (3) BAL culture results directed a change of therapy in 75.6% of positive episodes (34 of 45) and in 54.8% of all episodes of treatment failure (34 of 62); and (4) there was no difference in mortality among positives, negatives, and patients who changed therapy guided by BAL culture.

Black bean (Phaseolus vulgaris L.) as a protective agent against DNA damage in mice.

This study was designed to evaluate the toxicogenetic or protective effect of cooked and dehydrated black beans (Phaseolus vulgaris L.) in bone marrow and peripheral blood cells of exposed mice. The frequency of micronuclei detected using the bone marrow erythrocyte micronucleus test and level of DNA lesions detected by the comet assay were chosen as end-points reflecting mutagenic and genotoxic damage, respectively. Initially, Swiss male mice were fed with a 20% black bean diet in order to detect mutagenic and genotoxic activity. However, no increase in the frequency of bone marrow micronucleated polychromatic erythrocytes (MN PCEs) or DNA lesion in leukocytes was observed. In contrast, received diets containing 1, 10 or 20% of black beans, a clear, but not dose-dependent reduction in the frequency of MN PCEs were observed in animals simultaneously treated with cyclophosphamide, an indirect acting mutagen. Similar results were observed in leukocytes by the comet assay. Commercial anthocyanin was also tested in an attempt to identify the bean components responsible for this protective effect. However, instead of being protective, the flavonoid, at the highest dose administered (50 mg/kg bw), induced primary DNA lesion, as detected by the comet assay. These data indicate the importance of food components in preventing genetic damage induced by chemical mutagens, and also reinforce the role of toxicogenetic techniques in protecting human health.

Pharmacological antagonism of Anchietia salutaris extracts on the contraction induced by prostaglandin D2 and U46619 in guinea-pig lung parenchymal strips.

Anchietia salutaris tea is traditionally used in Brazil to treat allergies, suggesting it contains compounds with antagonistic activity on the allergic mediators. We have evaluated extracts and semi-purified fractions of Anchietia salutaris as a source of compounds having this type of antagonism on the contraction induced in guinea-pig lung parenchymal strips and on platelet aggregation and shape change. After 10 min pre-incubation dichloromethane extracts containing 30 or 100 microg mL(-1) inhibited the contraction induced by prostaglandin D2 (PGD2) in guinea-pig lung parenchymal strips with dose ratios (DR) of 0.76+/-0.14 and 0.93+/-0.19, respectively; the amount of inhibition depended both on the concentration and on the time of pre-incubation (DR after 30 min pre-incubation was 1.21+/-0.51). The dichloromethane extract and its semi-purified fractions also inhibited the contractions induced by U46619, a more potent, stable, synthetic agonist of thromboxane A2 (TxA2) prostanoid (TP) receptors, the receptors acted upon by PGD2 to produce lung contractions. The dichloromethane extract did not inhibit the lung parenchymal contractions induced by histamine, leukotriene D4 (LTD4) or platelet-activating factor (PAF). Platelet aggregation induced by U46619, adenosine 5'-diphosphate (ADP) or PAF was not inhibited by the dichloromethane extract. Indeed, the extract potentiated platelet aggregation induced by low concentrations of these agonists and also potentiated the shape change induced by U46619. These results imply that the dichloromethane extract of Anchietia salutaris and its semi-purified fractions contain an active principle that competitively inhibits TxA2 TP receptors, the stimulation of which causes lung parenchymal contraction. The inhibition seems to be selective for this receptor subtype, because the extract fails to inhibit platelet aggregation or shape change. This provides additional support of earlier reports suggesting the occurrence of TP receptor subtypes.

Effects of ethanol, acetaldehyde, and acetic acid on histamine secretion in guinea pig lung mast cells.

We studied the direct effects of ethanol and its metabolites on the guinea pig lung mast cell, and the alterations caused in the histamine release induced by different stimuli. Guinea pig lungs cells dispersed by collagenase were used throughout. High concentrations of ethanol (100 mg/ml), acetaldehyde (0.3-3 mg/ml) and acetic acid (3 mg/ml) induced histamine release that was not inhibited by sodium cyanide (0.3 mM). Lower concentration of ethanol (10 mg/ml) and acetic acid (0.3 mg/ml), but not acetaldehyde, inhibited the histamine release induced by antigen and ionophore A23187. The histamine release induced by phorbol 12-miristate 13-acetate (1 microM) was also inhibited by ethanol (10 mg/ml). Changes in the levels of calcium, glucose and phosphatidic acid did not influence the effect of ethanol. We conclude that high doses of ethanol, acetaldehyde, and acetic acid cause a cytotoxic histamine release by independent mechanisms. Low concentrations of acetic acid inhibit the histamine release by pH reduction. Ethanol acts by a generalized effect that is independent of calcium and glucose suggesting a nonspecific effect that, nevertheless, is not cytotoxic since it can be reversed by washing the cells.

[Legionella pneumophila associated with acute respiratory insufficiency. 1st isolation in Brazil]. / Legionella pneumophila associada a insuficiência respiratória aguda. Primeiro isolamento no Brasil (1).

Isolation of Legionella pneumophila sero-group 1 with serological evidence of present infection is reported from a 40 year-old male with serious respiratory infection which developed into acute respiratory failure. It was characterized by severe hypoxemia resistant to high inspired oxygen concentrations and radiographically by diffuse infiltrates in both lungs suggesting the clinical aspect of ARDS. Following the introduction of clindamycin, amikacin, ceftriaxone, volume-cycled ventilator and positive end expiratory pressure (PEEP) of 14 cm H2O, stabilization of clinical conditions and gradual recovery were achieved. Suspecting of legionellosis, blood and tracheal secretions specimens were collected for specific laboratory research. From tracheal secretion cultivated in BCYE medium, gran-negative bacilli were isolated and identified as Legionella pneumophila serogroup 1 through cultural and biochemical characteristics and direct immunofluorescence and slide agglutination tests. Serology (IFA) with blood samples collecting during the 1st, 3rd, 4th and 6th weeks of illness demonstrated antibody titers to the isolated microorganism of 128, 1024, 4096 and 8192, respectively. Definitive results were obtained during the patient's recovery. The authors emphasize: a) the demonstration of the presence of Legionella sp. as a pathogenic agent in Brazil; b) the importance of supportive care in the clinical outcome; c) the need of remembering this pathogen while making differential diagnosis of pneumonias and of continuing to pursue this etiology with specific laboratory methodology.

[Acute reduction of blood pressure: comparative study of nifedipine and clonidine]. / Redução aguda da pressão arterial: estudo comparativo entre nifedipina e clonidina.

PURPOSE: To compare the efficacy of clonidine and nifedipine in rapidly reducing blood pressure (BP). PATIENTS AND METHODS: Forty-four patients with arterial hypertension of any cause were selected and randomly divided in three groups: P group, consisting of 9 patients who received placebo; N group, consisting of 17 patients, 8 males, mean age of 47 years who received 10 mg of sublingual nifedipine; C group, consisting of 18 patients, 6 males, with mean age of 47 years who received 0.2 mg of clonidine per os. The BP were measured on admission and every 15 minutes for 1 hour. The final goal of treatment was to lower diastolic pressure in 20 mmHg or more. RESULTS: At the end of 60 minutes the nifedipine as well as clonidine were equally effective in lowering BP. The earliest manifestation of drug activity occurred at 25 minutes after administration. Side effects were noted only with clonidine in 23% of the cases, but without any clinical significance. CONCLUSION: Nifedipine as well as clonidine are equally effective in rapidly lowering BP with minimal side effects.

Effect of operator experience on the outcome of fiber post cementation with different resin cements.

OBJECTIVES: To evaluate the influence of operator experience (dentist vs student) and cementation system (Adper Scotchbond Multi-Purpose [SBMP] + RelyX ARC [1]; Adper Single Bond 2 [SB] + RelyX ARC [2] and RelyX U100 [3]) on the push-out bond strength (BS) of fiber post to radicular dentin. MATERIALS AND METHODS: The roots of 48 extracted human maxillary central incisors were prepared and divided into six groups (n=8), according to combination of the above factors. Glass fiber posts were cemented in accordance with the instructions of the manufacturer of each cementation system. After water storage at 37°C for one week, the roots were cross-sectioned into six 1-mm thick slices and the push-out test was performed (0.5 mm/min). Data were statistically analyzed by two-way analysis of variance and Tukey tests (α=0.05). The BS results obtained by dentist and student for each cementation system were compared using the Student t-test (α=0.05). RESULTS: Higher BS means were observed for the expert operators, irrespective of the cementation system used (p=0.006). RelyX U100 showed the highest bond strength, but it did not differ from SBMP + RelyX ARC. The Student t-test revealed that only RelyX U100 was not affected by the operator's experience. CONCLUSION: Within the limitations of this in vitro study, it can be concluded that the self-adhesive cement RelyX U100 showed the highest bond strength to the root canal in the student's group, and its performance was not affected by the operator's experience.

Efecto del grabado de la dentina radicular con ácido dietilenoaminotetra acético (EDTA) en la resistencia de unión de cementos autoadhesivos y autograbantes / Effect of engraving dietilenoaminotetra acetic acid (EDTA) root dentin in the resistance of cemented joints stickers and etching

Fue evaluado el efecto del grabado de la dentina radicular con EDTA en la resistencia de unión (RU) inmediata (IM) y después de termociclaje (TM) de un cemento autoadhesivo (AA) y uno auto grabador (AG) para la cementación de postes de fibra de vidrio. Fueron utilizadas 40 raíces de premolares humanos divididas en 4 grupos según tipo de cementación (n=10) G1 RelyX U100 (UC) según indicaciones del fabricante(IF); G2 grabado con EDTA 24% por 60 s antes de la aplicación del UC ; G3 Para Post Para Core Automix (PC) (IF)G4 - grabado con EDTA 24% por 60 s antes de la aplicación del (PC).Las raíces fueron cortadas en discos de 1 mm (dos discos por tercio radicular), la mitad de los discos fueron sometidos a ensayo expulsión IM (0,5mm/min) y la otra mitad después de TM (5º C e 55º C).los datos fueron analizados en test de ANOVA y Tukey (?=0,05).dando como resultado que el grabado con EDTA altero negativamente los valores de RU solo del tercio cervical en los dos cementos. Cuando fue comparado TM con IM con o sin aplicación de EDTA no se encontraron diferencias estadísticas significativas. Para todos los grupos testados.El cemento PC obtuvo mayores valores de RU (19,59 MPa) comparado con el UC (15,80 MPa).concluyéndose que el tratamiento con EDTA afecto la RU solo para el tercio cervical tanto en IM como TM en los dos cementos. El TM con o sin EDTA no afecto los valores de RU cuando comparado con IM

The effect of post-space treatment whit EDTA on the bond strength (BS) of fiber posts in different root regions was evaluated using two different type of resin cements Rely X U100 (UC) and Para Post Para Core automix (PC) . Fourty extracted premolars root canals were assigned to four groups of 10 roots each. G1 RelyX U100 (UC) according to manufacturer's instructions (MI), G2 etching whit EDTA 24% for 60 s before application of UC; G3 Para Post Para Core Automix (PC) MI; G4 Etching whit EDTA 24% for 60s before application off PC. After cementation the roots were sectioned in 6 slices 1mm each (two slides for root region) and randomly divided into two subgroups, depending on testing time immediate (IM) vs. after termocicling(TM) ), for expulsion test. Statistical analysis was performed with ANOVA and Tukey test (?=.05) No differences in BS was found among the groups whit and whit out EDTA 24 %. Etching whit EDTA adversely alter the BS values of the cervical third in the two cements. When TM was compared with IM with or without application of EDTA there was no statistically significant difference. For all groups tested, PC show higher values of BS (19.59 MPa) was compared with UC (15.80 MPa). Concluded that treatment with EDTA affected the BS only on the cervical third in both IM and TM in the two cements. The TM with or without EDTA did not affect the values of BS when compared with IM

Qualidade de méis produzidos por apicultores e méis provenientes de entrepostos registrados no Serviço de Inspeção Federal / Quality of honeys produced by beekeepers and honey from warehouses registered at the Federal Inspection Service

This study was carried out to compare the physiochemical quality of honeys from beekeepers in northern Zona da Mata, MG, and honeys from warehouses registered at the Serviço de Inspeção Federal (SIF) in the State of Minas Gerais. Physiochemical analysis involving 39 samples were done, with three samples from each of the 13 beekeepers and 18 samples from warehouses registered at SIF-MG. Differences in quality from the two origins occurred in: free acidity, ashes, hydroxymethylfurfural, apparent sucrose and insoluble solids, where honeys from warehouses were of better quality. In addition, for free acidity and insoluble solids, two samples from northern Zona da Mata showed values above the established by the Instrução Normativa n°11 relative to the year 2000, whereas the values for insoluble solids in 11 samples from northern Zona da Mata and 2 samples from warehouses were in disagreement with this legislation.