RESUMEN
This study focused on the simultaneous recovery of carbohydrates (CHO) and phosphorus (P) from Desmodesmus sp. biomass cultivated in municipal wastewater, through a sequential pretreatment. The pretreatment consisted first of ultrasound to trigger cell disruption followed by ozonation to recover CHO and P. For ozone pretreatment, three different parameters were considered: ozone concentration (9, 15, 21, 27, 36, and 45 mg O3/L), contact time (15, 25 and 35 min), and pH (8 and 11). The maximum simultaneous release of 84% of CHO and 58% of P was achieved at the experimental parameters of ozone concentration of 45 mg O3/L, contact time of 35 min, and pH of 11. Also, P was concentrated in solution by 8- to 14-fold with respect to municipal wastewater. The sequential pretreatment was conducted at alkaline pH of 11 and atmospheric conditions, which may considerably reduce energy demand and reagents, in comparison to a traditional hydrolysis pretreatment. The results found suggest that the sequential pretreatment could be feasible on a large scale.
Asunto(s)
Ozono , Aguas Residuales , Biomasa , Carbohidratos , FósforoRESUMEN
This study evaluates the effect of ultrasound and ozone pretreatments for the subsequent recovery of Desmodesmus sp. biocomponents-lipids, proteins, and carbohydrates-using a response surface methodology. Both pretreatments impact on the recovered lipids quality, solvent waste production and extraction time is analysed for process intensification purposes. For ultrasound pretreatment, independent parameters were energy applied (50-200â¯kWh/kg dry biomass), biomass concentration (25-75â¯g/L), and ultrasonic intensity (0.32 and 0.53â¯W/mL). While for ozone pretreatment, independent parameters were ozone concentration (3-9â¯mg O3/L), biomass concentration (25-75â¯g/L), and contact time (5-15â¯min). In the case of ultrasound pretreatment, recovery yield reached 97⯱â¯0.4%, 89⯱â¯3%, and 73⯱â¯0.6% for proteins, carbohydrates and lipids respectively. Given process required: energy applied of 50â¯kWh/kg dry biomass, 75â¯g/L of biomass concentration, 0.32â¯W/mL of ultrasonic intensity, and 56â¯min of time process. Ultrasound caused high cell disruption releasing all proteins, thereby obviating downstream processing for its recovery. Ozone pretreatment recovery yield was 85⯱â¯2%, 48⯱â¯1.4%, and 25⯱â¯1.3%, for carbohydrates, lipids and proteins respectively, under the following conditions: 9â¯mg O3/L of ozone concentration, 25â¯g/L of biomass concentration, and 5â¯min of contact time that depicts an energy consumption of 30.64â¯kWh/kg dry biomass. It was found that ultrasound and ozone pretreatments intensified the lysis and biocomponents recovery process by reducing solvent consumption by at least 92% and extraction time between 80% and 90% compared with extraction of untreated biomass biocomponents. Both pretreatments improve the composition of the recovered lipids. It was noted that the yield of neutral lipids increased from 28% to 67% for ultrasound pretreatment while for ozone pretreatment from 49% to 63%. The method used for lipid extraction may also have an effect but here it was kept constant.