RESUMEN
The authors describe a case with sciatic pain caused by a large extrapelvic aneurysm of the inferior gluteal artery. Differential diagnosis and treatment are discussed.
Asunto(s)
Aneurisma/complicaciones , Ciática/etiología , Aneurisma/diagnóstico , Diagnóstico Diferencial , Femenino , Humanos , Persona de Mediana Edad , Ciática/diagnóstico , Compresión de la Médula Espinal/diagnósticoAsunto(s)
Trasplante de Corazón , Inmunología del Trasplante , Enfermedad Aguda , Adulto , Animales , Arterias/patología , Azatioprina/uso terapéutico , Enfermedad Crónica , Vasos Coronarios/patología , Perros , Edema , Electrocardiografía , Corazón/efectos de la radiación , Hemorragia , Histocompatibilidad , Humanos , Isoenzimas , L-Lactato Deshidrogenasa/sangre , Masculino , Microscopía Electrónica , Persona de Mediana Edad , Monocitos , Miocardio/patología , Necrosis , Pericardio/irrigación sanguínea , Prednisona/uso terapéutico , Factores de Tiempo , Trasplante HomólogoAsunto(s)
Trasplante de Corazón , Corazón/efectos de la radiación , Efectos de la Radiación , Inmunología del Trasplante , Animales , Arritmias Cardíacas , Azatioprina/farmacología , Perros , Edema , Electrocardiografía , Corazón/efectos de los fármacos , Frecuencia Cardíaca , Mitocondrias Musculares , Miocardio/patología , Penicilinas/farmacología , Estreptomicina/farmacología , Trasplante HomólogoAsunto(s)
Histerosalpingografía , Infertilidad Femenina/diagnóstico , Aire , Anomalías Congénitas/diagnóstico , Trompas Uterinas , Femenino , Enfermedades de los Genitales Femeninos/diagnóstico , Humanos , Infertilidad Femenina/terapia , Métodos , Embarazo , Enfermedades Uterinas/diagnóstico , Útero/anomalíasAsunto(s)
Hipertensión Renal/diagnóstico , Hipertensión Renovascular/diagnóstico , Obstrucción de la Arteria Renal/diagnóstico , Adulto , Angioplastia de Balón , Antihipertensivos/uso terapéutico , Femenino , Furosemida/uso terapéutico , Humanos , Hipertensión Renovascular/cirugía , Hipertensión Renovascular/terapia , Calidad de Vida , Obstrucción de la Arteria Renal/cirugía , Obstrucción de la Arteria Renal/terapia , Renina/sangreRESUMEN
Proton and 13C chemical shift assignments are reported for the neuropeptide Met-enkephalin (ME) in both aqueous solution and in the presence of 50 mM sodium dodecyl sulfate (SDS). Rotating frame nuclear Overhauser enhancement spectroscopy was used to qualitatively describe interproton distances. These distances were then used as restraints in the distance geometry based molecular modeling program Dspace, developed by Hare Research to generate sets of conformations of ME. The resulting aqueous solution conformations of ME were determined to exhibit characteristic of an extended random-coil polypeptide with no distinguishable secondary structure. The resulting set of solution conformations of ME in the presence of 50 mM SDS exhibited characteristics of an amphiphilic type IV beta turn that are stabilized by hydrophobic aromatic-aromatic interactions between the side chains of Tyr1 and Phe4.
Asunto(s)
Encefalina Metionina/química , Secuencia de Aminoácidos , Espectroscopía de Resonancia Magnética , Micelas , Modelos Moleculares , Datos de Secuencia Molecular , Conformación Proteica , Dodecil Sulfato de Sodio , SolucionesRESUMEN
Ultraviolet-visible spectroscopy has been used as a rapid method to evaluate the hydrophobic interactions between a series of cationic and zwitterionic neuropeptides and dipeptides with the hydrophobic core of two membrane model systems; sodium dodecyl sulfate and lysophosphatidylcholine micelles. If a hydrophobic interaction occurs, a 1-nm bathochromic shift is observed in the uv-visible spectrum of the aromatic side chains when going from aqueous solution to a micellar solution. The aromatic residues of substance P, bradykinin, and Des-Arg9 bradykinin all exhibited the 1-nm bathochromic shift in the presence of sodium dodecyl sulfate while those of Met-enkephalin did not. The opposite effects were observed in the presence of lysophosphatidylcholine micelles.
Asunto(s)
Membranas/metabolismo , Neuropéptidos/metabolismo , Secuencia de Aminoácidos , Lisofosfatidilcolinas , Membranas/química , Micelas , Modelos Biológicos , Datos de Secuencia Molecular , Neuropéptidos/química , Dodecil Sulfato de Sodio , Espectrofotometría Ultravioleta , Agua/químicaRESUMEN
A videomicroscopic method was used to quantitatively analyze villous motility in the dog small intestine. The frequency and duration of villous contractions (retractions) were measured in the duodenum, midjejunum, and distal ileum under controlled conditions. A pronounced gradient of villous motility was evident along the bowel. The duodenum exhibited the highest frequency (7.3 +/- 0.1/min) and longest duration (2.6 +/- 0.1 s) of contraction; the jejunum exhibited an intermediate frequency and duration of contraction (4.0 +/- 0.1/min, 2.1 +/- 0.1 s), and the lowest values were measured in the ileum (2.0 +/- 0.1/min and 1.8 +/- 0.1 s). In contrast to the retraction movements, the frequency of pendular villous movements (whipping, swaying movements without shortening) was highest in the jejunum and lowest in the duodenum. The frequency and duration of villous contractions (retractions) remained relatively constant over a 2-h observation period. Reducing mucosal surface temperature from 38 to 30 degrees C caused the frequency of contraction to fall by 33% and the duration to increase by 106%. Varying the suffusate pH within the physiological range of 5.0-7.4 produced no significant effects on jejunal villous motility. Suffusion with glucose (140 and 280 mM) failed to alter villous motility. However, amino acid (15 and 30 mM) and fatty acid (10 mM) solutions significantly increased contraction frequency by 30-50% and 90%, respectively. The videomicroscopic method provides useful quantitative information, which should extend current knowledge regarding the regulation and physiological importance of villous motility.