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1.
Medicina (Kaunas) ; 60(6)2024 May 29.
Artículo en Inglés | MEDLINE | ID: mdl-38929515

RESUMEN

Background and Objectives: The purpose of the study was to analyze the relationships among several clinical factors and also the tumor topography and surgical strategies used in patients with colorectal cancer. Materials and Methods: We designed an analytical, observational, retrospective study that included patients admitted to our emergency surgical department and diagnosed with colorectal cancer. The study group inclusion criteria were: patients admitted during 2020-2022; patients diagnosed with colorectal cancer (including the ileocecal valve); patients who benefited from a surgical procedure, either emergency or elective. Results: In our study group, consisting of 153 patients, we accounted for 56.9% male patients and 43.1% female patients. The most common clinical manifestations were pain (73.2% of the study group), followed by abdominal distension (69.3% of the study group) and absence of intestinal transit (38.6% of the study group). A total of 69 patients had emergency surgery (45.1%), while 84 patients (54.9%) benefited from elective surgery. The most frequent topography of the tumor was the sigmoid colon, with 19.60% of the patients, followed by the colorectal junction, with 15.68% of the patients, and superior rectum and inferior rectum, with 11.11% of the patients in each subcategory. The most frequent type of procedure was right hemicolectomy (21.6% of the study group), followed by rectosigmoid resection (20.9% of the study group). The surgical procedure was finished by performing an anastomosis in 49% of the patients, and an ostomy in 43.1% of the patients, while for 7.8% of the patients, a tumoral biopsy was performed. Conclusions: Colorectal cancer remains one of the most frequent cancers in the world, with a heavy burden that involves high mortality, alterations in the quality of life of patients and their families, and also the financial costs of the medical systems.


Asunto(s)
Neoplasias Colorrectales , Procedimientos Quirúrgicos Electivos , Humanos , Masculino , Femenino , Neoplasias Colorrectales/cirugía , Estudios Retrospectivos , Anciano , Persona de Mediana Edad , Estudios de Cohortes , Anciano de 80 o más Años , Adulto , Urgencias Médicas
2.
Medicina (Kaunas) ; 59(5)2023 May 02.
Artículo en Inglés | MEDLINE | ID: mdl-37241107

RESUMEN

Introduction: Despite the improvement of early diagnosis methods for multiple pathological entities belonging to the digestive tract, bowel obstruction determined by multiple etiologies represents an important percentage of surgical emergencies. General data: Although sometimes obstructive episodes are possible in the early stages of colorectal cancer, the most commonly installed intestinal obstruction has the significance of an advanced evolutionary stage of neoplastic disease. Development of Obstructive Mechanism: The spontaneous evolution of colorectal cancer is always burdened by complications. The most common complication is low bowel obstruction, found in approximately 20% of the cases of colorectal cancer, and it can occur either relatively abruptly, or is preceded by initially discrete premonitory symptoms, non-specific (until advanced evolutionary stages) and generally neglected or incorrectly interpreted. Success in the complex treatment of a low neoplastic obstruction is conditioned by a complete diagnosis, adequate pre-operative preparation, a surgical act adapted to the case (in one, two or three successive stages), and dynamic postoperative care. The moment of surgery should be chosen with great care and is the result of the experience of the anesthetic-surgical team. The operative act must be adapted to the case and has as its main objective the resolution of intestinal obstruction and only in a secondary way the resolution of the generating disease. Conclusions: The therapeutic measures adopted (medical-surgical) must have a dynamic character in accordance with the particular situation of the patient. Except for certain or probably benign etiologies, the possibility of colorectal neoplasia should always be considered, in low obstructions, regardless of the patient's age.


Asunto(s)
Neoplasias Colorrectales , Obstrucción Intestinal , Humanos , Obstrucción Intestinal/etiología , Obstrucción Intestinal/cirugía , Neoplasias Colorrectales/complicaciones , Neoplasias Colorrectales/patología , Resultado del Tratamiento , Estudios Retrospectivos
3.
Medicina (Kaunas) ; 59(9)2023 Aug 23.
Artículo en Inglés | MEDLINE | ID: mdl-37763635

RESUMEN

Rhabdomyosarcoma is a rare tumor that is diagnosed mostly in children and adolescents, rarely in adults, representing 2-5% of all soft tissue sarcomas. It has four subtypes that are recognized: embryonal (50%), alveolar (20%), pleomorphic (20%), and spindle cell/sclerosing (10%). The diagnosis of rhabdomyosarcoma is based on the histological detection of rhabdomyoblasts and the expression of muscle-related biomarkers. Spindle cell/sclerosing rhabdomyosarcoma consists morphologically of fusiform cells with vesicular chromatin arranged in a storiform pattern or long fascicles, with occasional rhabdomyoblasts. Also, dense, collagenous, sclerotic stroma may be seen more commonly in adults. We present a rare case of an adult who presented to the hospital with a tumor in the left inguinal area, was first diagnosed with a left strangulated inguinal hernia and was operated on as an emergency, although the diagnosis was ultimately a spindle cell rhabdomyosarcoma of the inguinal region.

4.
Chirurgia (Bucur) ; 118(6): 642-653, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-38228596

RESUMEN

Introduction: Bilateral inguinal hernia can be safely repaired simultaneously, open or minimally invasive, in an elective sce-nario. The choice of surgical approach depends on the patient's status, hernia characteristics, surgeons and patient preferences. Whether age criteria should be considered when selecting between the two approaches is still a matter of debate. Considering that there is no consensus regarding the best repair in bilateral inguinal herniorrhaphy, the aim of the study is to perform an analysis regarding elective surgical approach of patients with bilateral inguinal hernias. Material and Methods: To study the relationship between exposure to an open versus laparoscopic approach in patients with bilateral inguinal hernia, we conducted a case-control study. In our retrospective analysis, cases (23 patients) were the open-approach hernia repair, and controls (82 patients) were laparoscopic hernia repair. We analyzed two sets of variables: first, related to patient characteristics (age 65 years, BMI 30 kg/m2, smoking habit, HTA status, COPD status, DM status, use of anticoagulants, presence of neoplastic status) and second, variables related to hernia features (inguinoscrotal hernia, recurrent hernia and complicated hernia). Results: The mean age for cases was 73.26 (Ã+-12.99) years and that of controls, was 56.48 (Ã+-15.15) years. Univariate analysis demonstrated four variables with statistical significance: age 65 years, inguinoscrotal hernia, neo-plastic status, and anticoagulant use. When introduced into the multivariate analysis, we noted that only two variables, age 65 years (OR=4.183, 95% CI [1.289, 13.572], p=0.017) and use of anticoagulants (OR=38.876, 95% CI [1.305, 1158.011], p=0.035) reached statistical significance. Conclusion: This study demonstrates that when we refer to bilateral inguinal hernia repair, patients aged 65 years are at risk of having an open procedure at least fourfold more than patients aged 65 years. In addition, the use of anticoagulants increases the risk of open hernia repair 38 times more than that of minimally invasive repair for the same age group. Interestingly, in our study, hernia characteristics were not found to be associated with open hernia repair and age 65 years. In our study we found that age 65 years is associated with electing open hernia repair over minimally invasive repair, which can be linked to age-related risk factors. Further re-search is needed to investigate the impact of age and age-related risk factors on surgical outcomes of bilateral inguinal hernia repair.


Asunto(s)
Hernia Inguinal , Laparoscopía , Humanos , Hernia Inguinal/cirugía , Estudios de Casos y Controles , Estudios Retrospectivos , Resultado del Tratamiento , Laparoscopía/métodos , Herniorrafia/métodos , Anticoagulantes
5.
Medicina (Kaunas) ; 59(1)2022 Dec 31.
Artículo en Inglés | MEDLINE | ID: mdl-36676724

RESUMEN

The COVID-19 pandemic has brought infectious and contagious diseases back to the forefront of medical concerns worldwide [...].


Asunto(s)
COVID-19 , Neoplasias , Humanos , Pandemias
6.
Chirurgia (Bucur) ; 116(6): 669-677, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34967712

RESUMEN

Post-traumatic morbid associations may evolve by themselves, or can interact, with effects of adding or potentiating gravity. Sometimes cumulative or potentiating effects are associated with clinical and paraclinical confusing manifestations induced by one of the components of the lesion complex, or by mutually aggravating effects. It is also the case of vertebromedular traumas associated with other post-traumatic lesions, where the neurological signal is distorted, and may induce particularities of associated lesions. Posttraumatic spinal shock, autonomic dysreflexia and motor, sensory and autonomous after effects, bring distortions of the neurogenic signal, which will significantly affect other traumatized territories, generating clinical manifestations and atypical reaction elements. The prototype of this post-traumatic morbid association is represented by the lesion complex vertebromedular trauma - abdominal trauma.


Asunto(s)
Disreflexia Autónoma , Traumatismos de la Médula Espinal , Humanos , Traumatismos de la Médula Espinal/complicaciones , Traumatismos de la Médula Espinal/diagnóstico , Resultado del Tratamiento
7.
Chirurgia (Bucur) ; 116(6 Suppl): S54-S68, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-35274612

RESUMEN

Introduction: Traumatic injuries are a major public health problem, being one of the main causes of death, the subsequent decline in the quality of life of the patients involved, as well as the costs of hospitalization in the health system. Material and Method: We conducted a 5-year retrospective study on 798 hospitalized patients with traumatic injuries, by collecting a series of general, clinical, imaging and biological parameters. Results: The results showed a higher proportion of male patients, the main lesion mechanism being represented by road accidents, followed by falls from another level. The most common traumatic intraabdominal parenchymal lesions were splenic, followed by hepatic and renal lesions. In addition to intraabdominal trauma, the lesion balance also included thoracic, spinal cord, head and limb injuries. A total of 288 patients underwent emergency surgery, but there were also cases of delayed surgical procedures or reoperations. We had 67 deceased patients. Conclusions: Trauma is a major public health problem, both socially and economically, being responsible for a significant number of deaths, but also in terms of the significant number of hospitalizations and the subsequent impact on the quality of life of those involved.


Asunto(s)
Accidentes de Tránsito , Calidad de Vida , Accidentes por Caídas , Humanos , Masculino , Estudios Retrospectivos , Resultado del Tratamiento
8.
Chirurgia (Bucur) ; 114(1): 73-82, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30830847

RESUMEN

Introduction: Diaphragmatic injuries are produced by blunt or penetrating thoracoabdominal trauma. They are potentially life-threatening due to the herniation of abdominal organs into the pleural cavities and severe associated lesions. The aim of this retrospective study was to analyse the clinical presentation and management of patients admitted with diaphragmatic rupture in our institution. Material and Method: We performed a 5-year retrospective study of patients admitted with acute blunt or penetrating diaphragmatic rupture in the Department of General Surgery of "Bagdasar- Arseni" Emergency Hospital. We have studied sex, age, mechanism of trauma, side-location, timeto-diagnosis, concomitant injuries, surgical treatment and outcome. Results: Fifteen patients (8 males, 7 females, mean age: 42 years) with diaphragmatic rupture (left-side: 13, right-side: 2) following blunt (8 patients) or penetrating (7) trauma were included. Patients with blunt diaphragmatic injury had larger tears and abdominal viscera herniation was observed in 6of these cases. A direct suture was performed for all 15 patients. Laparotomy was the surgical approach preferred in most of the cases. Mortality rate was 20%, mainly caused by severe associated lesions. Conclusion: Although traumatic diaphragmatic lesions are frequently associated with severe cerebral and thoraco-abdominal trauma that is also the main cause of death, a prompt diagnosis and treatment can lead to good outcome.


Asunto(s)
Diafragma/lesiones , Hernia Diafragmática Traumática/diagnóstico , Hernia Diafragmática Traumática/cirugía , Adulto , Diafragma/cirugía , Femenino , Hernia Diafragmática Traumática/etiología , Humanos , Masculino , Traumatismo Múltiple/complicaciones , Estudios Retrospectivos , Rotura , Resultado del Tratamiento , Heridas no Penetrantes/complicaciones , Heridas Penetrantes/complicaciones
9.
Chirurgia (Bucur) ; 113(3): 353-362, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29981666

RESUMEN

Frequently appearing as a complication of pancreatitis, pancreatic pseudocysts are rare appearances in current medical practice. Multiple therapeutical options are available, from minimum invasive methods to complex open surgical techniques. The indications of drainage include symptomatic pseudocysts, large pseudocysts along with the development of complications such as hemorrhage, secondary infection, and rupture. Choosing the best drainage technique is often a challenge. Multiple factors need to be considered in managing a pseudoocyst, including its location, dimension and the patient's general state and comorbidities. Thus, a therapeutical algorithm may be of great use in choosing the most feasible technique for the diagnosed lesion that is also patient-adapted.


Asunto(s)
Drenaje , Pancreatectomía , Seudoquiste Pancreático/etiología , Seudoquiste Pancreático/cirugía , Pancreatitis/complicaciones , Diagnóstico Diferencial , Drenaje/efectos adversos , Drenaje/métodos , Humanos , Seudoquiste Pancreático/diagnóstico por imagen , Factores de Riesgo , Resultado del Tratamiento
10.
Surg Innov ; 24(3): 223-232, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28193123

RESUMEN

AIM: The aim of this article was to report a new transomphalic extraperitoneal surgical technique for treatment of hydrocephalus, called ventriculoportal shunt. MATERIALS AND METHODS: We performed ventriculoportal shunt on an experimental animal (pig). The particularity of ventriculoportal shunt consists in the fact that the distal end of the catheter is inserted transomphalic extraperitoneally in the portal system through reopened umbilical vein. We present technical details regarding this new surgical technique. RESULTS AND DISCUSSION: The animal had favorable outcome, without any postoperative early or late morbidity. We discuss indications, contraindications, possible complications in humans and advantages of ventriculoportal shunt compared with ventriculocardiac and ventriculoperitoneal drainages and possibilities to avoid complications specific to classic shunt procedures. CONCLUSIONS: Ventriculoportal shunt is a new surgical technique for treatment of hydrocephalus. The distal end of the catheter introduced into reopened umbilical vein, drains cerebrospinal fluid into the portal system. Ventriculoportal shunt is safe and easy to perform. With ventriculoportal shunt specific complications of ventriculoperitoneal or ventriculocardiac drainages can be potentially avoided. Ventriculoportal shunt combines advantages of vascular shunt with those of having an immunological barrier for cerebrospinal fluid before entering the systemic circulation. Theoretically, indications for surgery are extended, and ventriculoportal shunt can be performed in patients former contraindicated for ventriculoperitoneal shunt. Further research is needed and this surgical technique must be performed on human subjects with hydrocephalus.


Asunto(s)
Hidrocefalia/cirugía , Derivación Ventriculoperitoneal/métodos , Adulto , Animales , Cateterismo , Humanos , Prótesis e Implantes , Porcinos , Venas Umbilicales/cirugía
11.
Neurol Neurochir Pol ; 51(4): 290-298, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28528738

RESUMEN

INTRODUCTION: Hydrocephalus represents impairment in cerebrospinal fluid (CSF) dynamics. If the treatment of hydrocephalus is considered difficult, the repeated revisions of ventriculo-peritoneal (VP) shunts are even more challenging. OBJECTIVE: The aim of this article is to evaluate the efficiency of ventriculo-epiplooic (VEp) shunt as a feasible alternative in hydrocephalic patients. MATERIAL AND METHODS: A technical modification regarding the insertion of peritoneal catheter was imagined: midline laparotomy 8-10cm long was performed in order to open the peritoneal cavity; the great omentum was dissected between its two layers; we placed the distal end of the catheter between the two epiplooic layers; a fenestration of 4cm in diameter into the visceral layer was also performed. A retrospective study of medical records of 15 consecutive patients with hydrocephalus treated with VEp shunt is also presented. RESULTS: Between 2008 and 2014 we performed VEp shunt in 15 patients: 5 with congenital hydrocephalus, 8 with secondary hydrocephalus and 2 with normal pressure hydrocephalus. There were 7 men and 8 women. VEp shunt was performed in 13 patients with multiple distal shunt failures and in 2 patients, with history of abdominal surgery, as de novo extracranial drainage procedure. The outcome was favorable in all cases, with no significant postoperative complications. CONCLUSIONS: VEp shunt is a new, safe and efficient surgical technique for the treatment of hydrocephalus. VEp shunt is indicated in patients with history of recurrent distal shunt failures, and in patients with history of open abdominal surgery and high risk for developing abdominal complications.


Asunto(s)
Ventrículos Cerebrales/cirugía , Derivaciones del Líquido Cefalorraquídeo/métodos , Hidrocefalia/cirugía , Epiplón/cirugía , Adolescente , Adulto , Anciano , Niño , Preescolar , Estudios de Factibilidad , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Derivación Ventriculoperitoneal , Ventriculostomía , Adulto Joven
12.
Chirurgia (Bucur) ; 111(3): 274-8, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27452942

RESUMEN

Osteosarcomas are the most frequent primary malignant bone tumors in children and adolescents. Like brain metastases in osteosarcomas, the bowel metastases are very rare. We present the case of a 23-year-old female patient, diagnosed and operated in 2008 of osteosarcoma at the tibia, for which she had sessions of neoadjuvant and adjuvant chemotherapy, but presented lungs metastases for which she underwent surgery in 2014. Then, in March 2015, she was diagnosed with an intracranial expansive process, an osteosarcoma metastasis, for which a total ablation of the tumor was performed during the early postoperatory period, being transferred to the General Surgery Clinic for abdominal pain, abdominal distention, vomiting, and lack of intestinal transit regarding faeces and intestinal gas. Both clinically and imagistically, the diagnosis was of bowel obstruction. This was the reason for performing surgery, thus discovering a bowel obstruction secondary to a metastasis of the terminal ileum and liver metastases that were confirmed as osteosarcoma metastases from an anatomopathological and immunohistochemical point of view. The bowel metastases and the osteosarcoma brain metastases are very rare entities and, their association, most often with young patients, is exceptional. However, bowel metastases must be taken into account as a possible cause of bowel obstruction in patients with osteosarcoma.


Asunto(s)
Neoplasias Óseas/patología , Neoplasias Encefálicas/secundario , Neoplasias Intestinales/secundario , Obstrucción Intestinal/etiología , Neoplasias Primarias Múltiples , Osteosarcoma/secundario , Neoplasias Peritoneales/secundario , Tibia , Adulto , Neoplasias Óseas/cirugía , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/cirugía , Femenino , Humanos , Neoplasias Intestinales/diagnóstico , Neoplasias Intestinales/cirugía , Obstrucción Intestinal/diagnóstico , Obstrucción Intestinal/cirugía , Osteosarcoma/diagnóstico , Osteosarcoma/cirugía , Neoplasias Peritoneales/diagnóstico , Neoplasias Peritoneales/cirugía , Resultado del Tratamiento
13.
Chirurgia (Bucur) ; 111(6): 517-521, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-28044956

RESUMEN

Periampullary duodenal diverticula are associated with the presence of common bile duct stones, being encountered more frequently with the increase of age. We present the case of a 76 years old female patient, who underwents emergency surgery for a perforated lithiasic gangrenous acute cholecystitis and for whom we perform a cholecystectomy and an external biliary drainage using a transcystic tube. Both preoperative and postoperative imaging and endoscopic examinations certify the presence of a periampullary duodenal diverticulum. Postoperative cholangiography performed on the transcystic tube raises the suspicion of retained common bile duct lithiasis. An endoscopic retrograde cholangiopancreatography is performed, initially failing to cannulate the common bile duct. A precut sphincterotomy fistula technnique is performed, using as reference a guide inserted on the transcystic tube, with the extraction of biliay sludge from the common bile duct, and with subsequently favorable development. Association between common bile duct lithiasis and a periampullary duodenal diverticulum may represent a therapeutic challenge because of the increased risk of failure of the endoscopic treatment.


Asunto(s)
Colangiopancreatografia Retrógrada Endoscópica , Colecistectomía , Divertículo , Enfermedades Duodenales/complicaciones , Enfermedades Duodenales/cirugía , Cálculos Biliares/complicaciones , Cálculos Biliares/cirugía , Anciano , Colecistectomía/métodos , Divertículo/diagnóstico , Divertículo/cirugía , Drenaje/instrumentación , Enfermedades Duodenales/diagnóstico , Femenino , Cálculos Biliares/diagnóstico , Gangrena/patología , Humanos , Resultado del Tratamiento
14.
Hepatogastroenterology ; 61(130): 463-8, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24901163

RESUMEN

BACKGROUND/AIMS: Pancreaticoduodenal resection is the procedure of choice for tumors of the pancreatic head and periampullary region. During pancreaticoduodenectomy resection, early neck division may be impossible or inadequate in case of hepatic artery anatomic variants, suspected involvement of the superior mesenteric vessels or intraductal papillary mucinous tumors. This paper aims to reveal the effectiveness of a modified posterior approach pancreaticoduodenectomy in selected indications and to assess the preliminary results. METHODOLOGY: We describe our early approach to the retropancreatic vasculature during pancreaticoduodenal resection by mesopancreas first dissection before any pancreatic or digestive transection. RESULTS: We used this approach in 45 patients. Thirty had hepatic artery anatomic variant. Hepatic artery reconstruction was required in 2 cases. In nine patients with intraductal papillary mucinous tumors the pancreaticoduodenal resection was extended to the body in 6 and totalized in 3 patients. In six patients with adenocarcinoma involving the portomesenteric axis, venous resection and reconstruction was necessary. CONCLUSIONS: Mesopancreas first dissection is useful tool in selected cases of pancreaticoduodenal resection to improve safety and radicality of the procedure.


Asunto(s)
Páncreas/anatomía & histología , Páncreas/irrigación sanguínea , Pancreaticoduodenectomía/métodos , Adulto , Anciano , Disección , Femenino , Arteria Hepática/patología , Arteria Hepática/cirugía , Humanos , Masculino , Arteria Mesentérica Superior/anatomía & histología , Persona de Mediana Edad , Complicaciones Posoperatorias
15.
Life (Basel) ; 14(7)2024 Jul 05.
Artículo en Inglés | MEDLINE | ID: mdl-39063604

RESUMEN

BACKGROUND: Surgical site infections (SSIs) represent a noteworthy contributor to both morbidity and mortality in the context of patients who undergo colorectal surgery. Several risk factors have been identified; however, their relative significance remains uncertain. METHODS: We conducted a meta-analysis of observational studies from their inception up until 2023 that investigated risk factors for SSIs in colorectal surgery. A random-effects model was used to pool the data and calculate the odds ratio (OR) and 95% confidence interval (CI) for each risk factor. RESULTS: Our analysis included 26 studies with a total of 61,426 patients. The pooled results showed that male sex (OR = 1.45), body mass index (BMI) ≥ 25 kg/m2 (OR = 1.09), American Society of Anesthesiologists (ASA) score ≥ 3 (OR = 1.69), were all independent risk factors for SSIs in colorectal surgery. Conversely, laparoscopic surgery (OR = 0.70) was found to be a protective factor. CONCLUSIONS: The meta-analysis conducted revealed various risk factors, both modifiable and non-modifiable, associated with surgical site infections (SSIs) in colorectal surgery. These findings emphasize the significance of targeted interventions, including optimizing glycemic control, minimizing blood loss, and using laparoscopic techniques whenever feasible in order to decrease the occurrence of surgical site infections in this particular group of patients.

16.
Biomolecules ; 14(3)2024 Mar 19.
Artículo en Inglés | MEDLINE | ID: mdl-38540784

RESUMEN

A hybrid material, consisting of commercially available nitrocellulose (NC) membrane non-covalently modified with amino-polyethylene glycol functionalized reduced graphene oxide (NH2-PEG-rGO) nanoparticles, was successfully synthesized for oligonucleotide extraction. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the modification of the NC membrane, revealing characteristic peaks of both compounds, i.e., NC and NH2-PEG-rGO. Scanning Electron Microscopy (SEM) exhibited morphological changes in the NC/NH2-PEG-rGO hybrid membrane, marked by the introduction of NH2-PEG-rGO particles, resulting in a distinctly smothered surface compared to the porous surface of the NC control membrane. Wettability assays revealed hydrophobic behavior for the NC/NH2-PEG-rGO hybrid membrane, with a water contact angle exceeding 90°, contrasting with the hydrophilic behavior characterized by a 16.7° contact angle in the NC membrane. The performance of the NC/NH2-PEG-rGO hybrid membrane was evaluated for the extraction of ssDNA with fewer than 50 nucleotides from solutions containing various ionic species (MnCl2, MgCl2, and MnCl2/MgCl2). The NC/NH2-PEG-rGO hybrid membrane exhibited optimal performance when incubated in MgCl2, presenting the highest fluorescence emission at 525 relative fluorescence units (r.f.u.). This corresponds to the extraction of approximately 610 pg (≈13%) of the total oligo-DNA, underscoring the efficacy of the pristine material, which extracts 286 pg (≈6%) of oligo-DNA in complex solutions.


Asunto(s)
Grafito , Óxidos , Óxidos/química , Colodión , Grafito/química , Agua , ADN de Cadena Simple , Extracción en Fase Sólida
17.
Maedica (Bucur) ; 19(1): 195-200, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38736918

RESUMEN

Inguinoscrotal hernia (ISH) is an entity for which treatment is under debate. Open surgery is the standard approach, but the laparoscopic technique has a great outcome when used by experienced surgeons. Seroma is one of the complications following laparoscopic hernia repair for these scrotal hernias, which is due to the large hernia sac, usually transected, leaving in place a remnant of the sac. Conservative measures can be applied for the treatment of seroma; however, in symptomatic cases, puncture and aspiration of fluid are recommended. Sometimes, these seromas can reach a large size and require surgery to remove the remnant sac. Herein, we report the case of a 49-year-old male with a large seroma that occurred four days after the laparoscopic approach - total extraperitoneal repair (TEP) for inguinoscrotal hernia. Conservative measures failed, and because the symptoms were not alleviated, reintervention was opted for. The distal sac was excised and the patient recovered uneventfully. The six-month follow-up did not show any recurrence of the hernia or seroma. We emphasize the importance of sac management in large ISH cases and discuss seromas after laparoscopic hernia repair in such instances.

18.
J Surg Case Rep ; 2024(4): rjae232, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38605699

RESUMEN

Cryptorchidism is defined as the extra-scrotal position of the testes. It is a common disorder in male children, but rarely in adult patients. The association of cryptorchidism with hernia is a common finding in childhood, but is not frequent in adults or the elderly. Herein, we report a series of three cases (28-, 24-, and 34-year-old men) of adult inguinal hernia combined with cryptorchidism successfully managed by laparoscopic surgery under the same operative view. Laparoscopic transabdominal preperitoneal repair and orchiectomy were performed in all patients. No complications occurred in the postoperative period, and the patients were discharged on the first or second postoperative day. Pathological examination of the specimens revealed atrophic testes without malignancy. No hernia recurrence was observed during follow-up. The laparoscopic approach in the combined pathology of inguinal hernia and cryptorchidism is feasible in adult patients and has multiple advantages in terms of diagnosis and management.

19.
Rom J Morphol Embryol ; 64(3): 399-410, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37867357

RESUMEN

AIM: The authors set out to evaluate the correlations between three of the main morphological aortic parameters (elastic fibers - FE, collagen fibers - FCOL, and smooth muscle fibers - FM) and the cause of death. MATERIALS AND METHODS: Study groups included 25 cases died of a vascular disease (V_P), 37 cases died of a non-vascular disease (NV_P) and 28 cases died of a violent/suspect non-pathological cause of death (V_Dth), the latter group representing also the control group. Four aortic cross-sections (base, arch, thoracic, and abdominal regions) were collected during autopsy from the selected cases, fixed in 10% buffered formalin and first of all photographed together with a calibrating ruler. Then, they were embedded in paraffin, sectioned off at 4 µm and stained with Hematoxylin-Eosin (HE) and Orcein. The obtained histological slides were transformed into virtual slides. Fibrillary components amounts were using a custom-made software, developed in MATLAB (MathWorks, USA). Statistical tools used were Pearson's correlation test, t-test (two-sample assuming equal variances) and one-way analysis of variance (ANOVA) test. RESULTS AND DISCUSSIONS: The amounts of the three fibrillary components of the aortic tunica media had a synchronous variation in all aortic regions in each of the three groups, excepting FCOL in the group of patients died from vascular pathology, which presented only a trend of synchronous variation along the aorta. FE had their lowest values and FCOL had their highest values in patients died from vascular pathology. FCOL had always higher levels than FE in people died from any pathological condition, vascular or non-vascular. FM had always at least two times lower level than that of the other types of fibers, regardless of whether the person died due to a pathological condition or not. CONCLUSIONS: The different pathological conditions causing death are influencing the fibrillary composition of aortic tunica media. Further studies are required to reveal other changes in the morphology of aortic wall in particular and vascular wall in general that could be related with different pathological conditions affecting the entire organism.


Asunto(s)
Aorta , Fludrocortisona , Humanos , Causas de Muerte , Aorta/patología , Túnica Media/patología , Túnica Íntima/patología
20.
Maedica (Bucur) ; 18(4): 598-606, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-38348087

RESUMEN

Introduction: Bilateral inguinal hernia is a distinct entity in the inguinal hernia category. Open and minimally invasive techniques for the treatment of bilateral inguinal hernia have been previously described. If resources and surgeon expertise are available, guidelines recommend laparoscopic repair for this entity. Methods:We analyzed data from 83 patients who underwent laparoscopic inguinal hernia repair (total extraperitoneal repair - TEP) of 158 hernias (146 inguinal hernias and 12 other types). Patients had bilateral symptomatic hernias. Results:Male predominance, with a mean age of 56.7 years, was noted. Lateral hernias (according to EHS classification) were prevalent (71.08%). In the majority of cases (77.11%), meshes made up of a custom polypropylene monofilament mesh were used, followed by Bard 3D Max mesh and Ultralight mesh. Regarding postoperative complications, seroma was the most frequently encountered one in our series (7.23%), followed by urinary retention and 'feeling' of mesh (2.41%). Hydrocele, wound hematoma, cord hematoma and chronic pain were seen in 1.20% of patients. No wound infections were observed. The average operative time was 97.77 minutes (SD=17.08); when associated surgery was present, it prolonged the operative time, and we found statistical significance (p=0.002). Similarly, the presence of recurrent hernia extended the operative time, which was found to be statistically significant (p=0.003). The conversion rate in our data was 2.41%. Drainage, which was performed in 13 patients (15.66%), decreased the incidence of complications, especially seroma (p=0.026). The mean length of hospital stay was 2.93 days (SD=1.81), with most of the patients having been discharged on the second postoperative day (37.35%). Only one recurrence was identified (1.20%). Conclusion:The laparoscopic approach for bilateral inguinal hernia treatment is feasible and has been proven to be advantageous. Our study emphasizes that the TEP procedure has low rates of complications, conversion and recurrence; hence, we recommend bilateral hernia repair.

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