Occurrence of polycyclic aromatic hydrocarbons in the Yellow River delta: Sources, ecological risks, and microbial response.

This research investigated the distribution, sources, and ecological risks of polycyclic aromatic hydrocarbons (PAHs) in the Yellow River Delta (YRD), China, emphasizing the response of soil microorganisms. The study involved quantitative analyses of 16 PAHs specified by the U.S. Environmental Protection Agency (USEPA) in both water and soil, utilizing metagenomic technique to determine the response of microbial communities and metabolism within the soil. Results noted that PAHs in the water mainly originate from pyrogenic source and in the soil originate from mixture source, with higher concentrations found in wetland areas compared to river regions. The ecological risk assessment revealed low-to-moderate risk. Microbial analysis demonstrated increased diversity and abundance of bacteria associated with PAHs in areas with higher PAHs pollution. Metagenomic insights revealed significant effects of organic carbon on PAHs degradation genes (ko00624 and ko00626), as well as significant differences in specific metabolic pathways including phenanthrene degradation, with key enzymes showing significant differences between the two environments. The study underscores the importance of understanding PAHs distribution and microbial responses to effectively manage and mitigate pollution in estuarine environments.

Highly efficient phosphorous removal in constructed wetland with iron scrap: Insights into the microbial removal mechanism.

Excessive phosphorus (P) in surface water can lead to serious eutrophication and economic losses. Iron-based constructed wetland (CW) is considered as a promising solution to eliminate P effectively due to the advantage of low-cost. However, there is limited available information on the microbial removal mechanism of P in iron-based CW up to now. Therefore, CW with iron scrap was constructed to investigate the treatment performance and microbial removal mechanism in this study. Results showed that efficient and stable P removal (97.09 ± 1.90%) was achieved in iron scrap-based CW during the experiment period, which was attributed to the precipitation of iron and P and improved microbially mediated P removal. Metagenomic analysis showed that microbial diversity was enhanced and phosphate accumulating organisms (e.g., Dechloromonas and Tetrasphaera) were enriched in CW with iron scrap, which explained higher P removal reasonably. In addition, the abundance of genes involved in the P starvation (e.g., phoB), uptake and transport (e.g., pstB) were enhanced in iron scrap-based CW. Enrichment analysis demonstrated that phosphotransferase pathway was also significantly up-regulated in CW with iron scraps, indicating that the energy supply of microbial P removal was enhanced. These findings provide a better understanding of the microbial removal mechanism of P in iron-based CW.

MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma.

BACKGROUND: Non-small cell lung cancer (NSCLC) is still one of the diseases with the highest mortality and morbidity, and lung adenocarcinoma (LUAD) accounts for more than half of all NSCLC cases in most countries. miRNA can be used as a potential biological marker and treatment for lung adenocarcinoma. However, the effect of miR-937-3p to the invasion and metastasis of LUAD cells is not clear. METHODS: miRNA microarray is used to analyze the expression of miRNA in lung adenocarcinoma tissue. Transwell migration, Wound-healing assay and Western blot analysis are used to analyze cell migration, invasion and epithelial-mesenchymal transition (EMT) capabilities. Tube formation is used to assess angiogenesis ability. In addition, dual luciferase reporter gene detection is used to identify the potential binding between miRNA and target mRNA. In vivo experiments were performed on male NOD/SCID nude mice by tail vein injection to establish a transplanted tumor model. The CHIP experiment is used to verify the transcription factors of miRNA. RESULT: In our study, miR-937-3p was high-regulated in LUAD cell lines and tissues, and its expression level was related to tumor progression. We found that miR-937-3p high-expression has an effect on cell invasion and metastasis. In molecular mechanism, miR-937-3p causes SOX11 reduction by directly binding to the 3'-UTR of SOX11.In addition, MYC affects miR-937-3p transcription by binding to its promoter region. CONCLUSIONS: Our research shows that miR-937-3p is mediated by MYC and can control the angiogenesis, invasion and metastasis of LUAD by regulating SOX11, thereby promoting the progress of LUAD. We speculate that miR-937-3p can be used as a therapeutic target and potential biomarker for LUAD.

Optimizing agricultural biomass application to enhance nitrogen removal in vertical flow constructed wetlands for treating low-carbon wastewater.

Agricultural biomass waste in rural areas has been identified as an economical solid carbon sources in constructed wetlands (CWs) for treating low C/N ratio domestic sewage. However, little information is available regarding its optimal utilization as a media amendment for enhancing nitrogen removal in CWs. In this study, vertical flow CWs with different walnut peel amendment proportions (0%, 25%, 50%, 75%) were developed to explore the effects of biomass dosage on the treatment performance, nitrous oxide (N2O) emission and microbial metabolites. Results showed that the addition of biomass significantly enhanced the denitrification performance in all CWs, and the higher total nitrogen (TN) removal efficiency (91.14-97.16%) was achieved in CWs with the optimal dosage of 25%. While the addition of biomass resulted in a slight increase in N2O emission (20.56-270.13 µg m-2 h-1) compared with control systems. Additionally, the biomass addition increased the accumulation of extracellular polymeric substances (EPS) by facilitating microbial processes. Higher total EPS production was observed in CW with 25% biomass, and the proportion of tightly bound EPS (48%) dominated in the total EPS in different CWs.

Distributions and interactions of dissolved organic matter and heavy metals in shallow groundwater in Guanzhong basin of China.

Groundwater as the primary source of fresh water particularly in semi-arid regions is heavily threatened by various pollutants such as dissolved organic matter (DOM) and heavy metals due to anthropogenic activities. In this study, 113 shallow groundwater samples were collected from Guanzhong basin of China to explore spatial distributions and interactions of DOM and heavy metals (Fe, Mn and Cu). Fluorescence excitation-emission spectrophotometry with parallel factor analysis showed that DOM in groundwater mainly contained three humic-like and two protein-like substances with an average dissolved organic carbon (DOC) concentration of 12.85 mg L-1. Average Mn and Cu concentrations in groundwater were 19.92 µg L-1 and 7.05 µg L-1 with an increasing trend from west to east, whereas Fe concentration in central regions was much higher (34.23 µg L-1). Structural equation modeling analysis indicated that DOM in groundwater could be significantly affected by surface water, and heavy metals were influenced by urbanization. Moreover, DOM could strongly influence the bioavailability, migration, and transformation of Mn in groundwater. These findings would be beneficial for the effective utilization and protection of groundwater resources.

Circular RNA LPAR3 sponges microRNA-198 to facilitate esophageal cancer migration, invasion, and metastasis.

In this study, we explored expression and functions of circular RNA LPAR3 (circLPAR3) in esophageal squamous cell carcinoma (ESCC). The differential expression of circular RNAs (circRNAs) in 10 ESCC and corresponding paracarcinoma tissues was analyzed through circRNA microarray, then the candidate circRNAs were detected and verified through quantitative RT-PCR, and a novel circRNA was screened, which was circLPAR3. Circular RNA LPAR3 showed apparently high expression in ESCC tissues and cells, which was closely correlated with the clinical stage and lymph node metastasis of ESCC patients. Circular RNA LPAR3 was mainly located in the cytoplasm of ESCC cells, which was more stable than the baseline gene. Circular RNA LPAR3 upregulated MET gene expression through sponge adsorption of microRNA (miR)-198, activated the RAS/MAPK and the PI3K/Akt pathways, and promoted ESCC cell migration, invasion, and metastasis in vivo and in vitro. However, it had no effect on ESCC cell proliferation. Circular RNA LPAR3 can regulate the miR-198-MET signal axis to promote the migration, invasion, and metastasis of esophageal cancer cells, which can thereby serve as a potential diagnostic and therapeutic target of esophageal cancer.

Recent advances in the enhanced nitrogen removal by oxygen-increasing technology in constructed wetlands.

Constructed wetland has attracted more and more attention for wastewater purification due to its low construction cost and convenient operation recently. However, the unique waterflooding structure of constructed wetland makes the low dissolved oxygen level, which limits the effect of nitrogen removal in the system. Therefore, it is necessary to develop the oxygen-increasing technology to overcome the drawback in constructed wetlands. In this review, the mechanism of nitrogen removal in constructed wetland is discussed and oxygen is main influence factor is concluded. In addition, oxygen-increasing technologies in recent advances which improve the nitrogen removal efficiency greatly, are emphatically introduced. Finally, some future perspectives about oxygen-increasing techniques are also put forward in order to provide reference for further research and engineering application.

MicroRNA-1204 promotes cell proliferation by regulating PITX1 in non-small-cell lung cancer.

MicroRNA-1204 (miR-1204), a member of the PVT1 region, may improve B cell differentiation and metastasis in breast cancer. However, the role of miR-1204 in non-small-cell lung cancer (NSCLC) and its mechanism remain unclear. The GEO public database was first employed to find differentially expressed genes. The expression level of miR-1204 in patient tissues and NSCLC cell lines was determined using qRT-PCR. Cell proliferation assays were performed to investigate the impact of miR-1204 on cell growth. Bioinformatics analysis and dual-luciferase reporter assays were conducted to find potential target genes. Finally, we performed in vivo experiments to identify the effect of miR-1204 on tumor formation in nude mice. It was first found that miR-1204 was overexpressed in NSCLC tissues and cells. miR-1204 increased the proliferation of NSCLC cells and reduced cell cycle arrest in vitro. PITX1 (paired like homeodomain 1) was found as a potential target gene. In addition, PITX1 was also found to be low in expression in NSCLC tissues and cells. To show that PITX1 reversed the function of miR-1204 in promoting proliferation, confirmatory experiments were performed. Moreover, high miR-1204 and low PITX1 expression was highly correlated with tumor size, lymph node metastasis, and the TNM stage in patients diagnosed with NSCLC. Our results suggested that upregulated miR-1204 in NSCLC is associated with NSCLC progression and promotes NSCLC cell proliferation by downregulating PITX1. miR-1204 may act as a poor prognostic factor and a potential therapeutic target for NSCLC.

Electrically Switched Ion Exchange Based on Carbon-Polypyrrole Composite Smart Materials for the Removal of ReO4- from Aqueous Solutions.

A simple and rapid process of ReO4- (as a surrogate of TcO4-) removal from aqueous solutions based on the electrically switched ion exchange (ESIX) method has been demonstrated in this work. Activated carbon-Polypyrrole (AC-PPy) was synthesized from activated carbon and pyrrole by electrodeposition method which was served as an electrically switched ion exchanger for ReO4- removal. The characterization results show that the AC-PPy composite exhibited an excellent loading capacity and a high stability for ions uptake and release. Chronoamperometric studies show that the ESIX treatment could be completed within 60 s, demonstrating the rapid uptake and release of ions. Uptake and release of ReO4- was verified by electrochemical quartz crystal microbalance with dissipation shift (EQCMD) studies. By modulating the electrochemical potential of the AC-PPy, the uptake and release of ReO4- ions can be controlled. Similar trends of uptake and release of ReO4- were observed in cyclic voltammetry (-0.4 to 0.8 V) for five cycles with the EQCMD. X-ray photoelectron spectroscopy (XPS) confirmed the process of ReO4- removal in the AC-PPy composite. Conclusively, the smart material shows excellent efficiency and selectivity for the removal of ReO4- from aqueous solutions.

Intensified nutrients removal in constructed wetlands by integrated Tubifex tubifex and mussels: Performance and mechanisms.

The synergy of Tubifex tubifex (T. tubifex) and mussels on SFCWs (named SFCW-MT) performance was well studied in laboratory throughout a year. The SFCW-MT were steady operated with high TN and TP treatment, with the removal efficiencies of 37.85 ±â€¯5.22% and 39.26 ±â€¯5.20% even in winter. The mussels had excellent NH4-N removal efficiency, and avoid the shortage of NH4-N removal with T. tubifex in winter. Simultaneously, the SFCW-MT improved the NO3-N treatment by 51% than that in control group. The plant growth was improved in SFCW-MT, which reflected in the improvement of total chlorophyll contents and plant heights. The N and P absorbed by wetland plants and adsorbed by substrate were both increased with mussels. Microbial analysis results revealed that, the mussels could keep the abundance of nitrifiers despite the negative effect of T. tubifex. On that basis, the improved proportions of denitrifiers (Firmicutes) have a significantly recognized role in NO3-N transformation in SFCW-MT. The gut and membrane sections of mussels, as well as T. tubifex, also has proportions of denitrifiers and part of nitrifiers, and thus changed the microbial community in substrate. This evidence indicated that the co-existence of T. tubifex and mussels have potential application for simultaneous removal of NH4-N and NO3-N in CWs.

Rapid and efficient removal of Pb(II) from aqueous solutions using biomass-derived activated carbon with humic acid in-situ modification.

This study developed an humic acid (HA) in-situ modified activated carbon adsorbent (AC-HA) for the rapid and efficient removal of Pb(II) from aqueous media, and adsorption mechanisms are discussed. The physicochemical characteristics of activated carbons (AC) were investigated via N2 adsorption/desorption, scanning electron microscopy (SEM), Boehm's titration method and Fourier transform infrared spectroscopy (FTIR). AC-HA exhibited richer oxygen-containing functional groups than the original AC. In addition, the removal performance of AC-HA (250.0mg/g) toward Pb(II) was greatly improved compared with the original AC (166.7mg/g). The batch adsorption study results revealed that the Pb(II) adsorption data were best fit by the pseudo-second-order model of kinetics and Langmuir isotherm of isothermals, and therefore, the effect of the solution pH was studied. The superior performance of AC-HA was attributed to the HA modification, which contains numbers of groups and has a strong π-π interaction binding energy with AC and Pb(II) species. The adsorption mechanisms were confirmed via the XPS study. More importantly, the modified method is simple and has a low cost of production.

The crucial factor for microplastics removal in large-scale subsurface-flow constructed wetlands.

Constructed wetlands (CWs) are an effective method for removing microplastics (MPs). Nevertheless, the understanding of the impact of various parameters on MPs removal within CWs remains incomplete. Through field investigations of large-scale CWs and the application of machine learning methods with an interpretable attribution technique (the Shapley Additive Explanation), we investigated the critical factors influencing MPs removal within CWs. The MPs abundance in the influent and the inlet of Z-CW (400.1 ± 20.8 items/L and 699.6 ± 50.6 items/kg) was significantly higher compared to that in M-CW (138.8 ± 20.5 items/L and 166.5 ± 36.8 items/kg), with no significant difference observed in the effluent. The primary characteristic of MPs is their fibrous and transparent appearance. The MPs removal range from 87.9% to 95.5 %, influenced by the types and characteristics of MPs, physical and chemical parameters, biofilms, and different processes. Among these factors, dissolved organic carbon with high humic content, aromaticity, and carboxyl abundance may serve as a crucial factor in MPs removal. The results of this study highlight the significance of physical and chemical parameters for the MPs removal in CWs, providing the necessary theoretical data for the construction of future large-scale engineering applications.

Critical role of benthic fauna in enhancing nanoplastics removal in constructed wetland: Performance, fate and mechanism.

The presence of nanoplastics (NPs) in wastewater poses a considerable risk to ecosystems. Although constructed wetlands (CWs) have the potential to removal NPs, their efficiency is limited by insufficient consideration of ecosystem integrity. Herein, three typical benthic fauna (Corbicula fluminea, Chironomus riparius and Tubifex tubifex) were added to CWs to improve the ecological integrity of CWs, and further enhance the ecological benefits. Results indicated that the addition of C. fluminea, C. riparius and T. tubifex increased NPs removal by 19.14 %, 17.02 %, and 15.76 % than that without benthic faunas, respectively. Based on fluorescence signal analysis, the presence of benthic fauna could intake NPs, and enhanced the adsorption of NPs by plants. The addition of C. fluminea significantly increased catalase (1541.82 ± 41.35 U/g), glutathione S-transferase (0.34 ± 0.02 U/g), and superoxide dismutase (116.33 ± 6.91 U/g) activities (p < 0.05) as a defense mechanism against NPs-induced oxidative stress. Metagenomic analysis revealed that the abundances of key enzymes involved in glycolysis, the tricarboxylic acid cycle, and polystyrene metabolism pathways were increased when C. fluminea was added, corresponding to the microbial degradation of NPs. Overall, the results of this study implied that the benthic fauna can efficiently remove NPs from wastewater in CWs.

Biotic/abiotic transformation mechanisms of phenanthrene in iron-rich constructed wetland under redox fluctuation.

Iron-rich constructed wetlands (CWs) could promote phenanthrene bioremediation efficiently through biotic and abiotic pathways, which have gained increasing attention. However, the biotic/abiotic transformation mechanisms of trace organic contaminants in iron-rich CW are still ambiguous. Herein, three CWs (i.e., CW-A: Control; CW-B: Iron-rich CW, CW-C: Iron-rich CW + tidal flow) were constructed to investigate the transformation mechanisms of phenanthrene through Mössbauer spectroscopy and metagenomics. Results demonstrated CW-C achieved the highest phenanthrene removal (94.0 %) and bacterial toxicity reduction (92.1 %) due to the optimized degradation pathway, and subsequently achieved the safe transformation of phenanthrene. Surface-bound/low-crystalline iron regulated hydroxyl radical (·OH) production predominantly, and its utilization was promoted in CW-C, which also improved electron transfer capacity. The enhanced electron transfer capacity led to the enrichment of PAH-degrading microorganisms (e.g., Thauera) and keystone species (Sphingobacteriales bacterium 46-32) in CW-C. Additionally, the abundances of phenanthrene transformation (e.g., EC:1.14.12.-) and tricarboxylic-acid-cycle (e.g., EC:2.3.3.1) enzyme were up-regulated in CW-C. Further analysis indicated that the safe transformation of phenanthrene was mainly attributed to the combined effect of abiotic (·OH and surface-bound/low-crystalline iron) and biotic (microbial community and diversity) mechanisms in CW-C, which contributed similarly. Our study revealed the essential role of active iron in the safe transformation of phenanthrene, and was beneficial for enhanced performance of iron-rich CW.

Natural product fargesin interferes with H3 histone lactylation via targeting PKM2 to inhibit non-small cell lung cancer tumorigenesis.

Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors. There is an urgent need to find more effective drugs that inhibit NSCLC. Fargesin (FGS) has demonstrated anti-tumor effects; however, its efficacy and the molecular mechanism of inhibiting NSCLC are unclear. Herein, we investigated FGS' inhibitory effects on NSCLC by CCK8 and EdU assays and cell cycle analysis of A549 cells in vitro and in a nude mouse tumor transplantation model in vivo. FGS (10-50 µM) significantly inhibited cell proliferation and down-regulated expression levels of CDK1 and CCND1. Transcriptomic analysis showed that FGS regulated the cell metabolic process pathway. Differential metabolites with FGS treatment were enriched in glycolysis and pyruvate pathways. Cell metabolism assay were used to evaluate the oxygen consumption rate (OCR), Extracellular acidification rate (ECAR) in A549 cells. FGS also inhibited the production of cellular lactate and the expression of LDHA, LDHB, PKM2, and SLC2A1. These genes were identified as important oncogenes in lung cancer, and their binding to FGS was confirmed by molecular docking simulation. Notably, the over-expression and gene silencing experiments signified PKM2 as the molecular target of FGS for anti-tumorigenesis. Moreover, the H3 histone lactylation, were correlated with tumorigenesis, were inhibited with FGS treatment. Conclusively, FGS inhibited the aerobic glycolytic and H3 histone lactylation signaling pathways in A549 NSCLC cells by targeting PKM2. These findings provide evidence of the therapeutic potential of FGS in NSCLC.

Efficient stabilization of arsenic migration and conversion in soil with surfactant-modified iron-manganese oxide: Environmental effects and mechanistic insights.

The use of iron-manganese oxide (FMO) as a promising amendment for remediating arsenic (As) contamination in soils has gained attention, but its application is limited owing to agglomeration issues. This study aims to address agglomeration using surfactant-modified FMO and investigate their stabilization behavior towards As and resulting environmental changes upon amendments. The results confirmed the efficacy of surfactants and demonstrated that cetyltrimethylammonium-bromide-modified FMO significantly reduced the leaching concentration of As by 92.5 % and effectively suppressed the uptake of As by 85.8 % compared with the control groups. The ratio of the residual fraction increased from 30.5-41.6 % in unamended soil to 67.9-69.2 %. The number of active sites was through the introduction of surfactants and immobilized As via complexation, ion exchange, and redox reactions. The study also revealed that amendments and the concentration of As influenced the soil physicochemical properties and enriched bacteria associated with As and Fe reduction and changed the distribution of C, N, Fe, and As metabolism genes, which promoted the stabilization of As. The interactions among cetyltrimethylammonium bromide, FMO, and microorganisms were found to have the greatest effect on As immobilization.

New insights for simultaneous nutrient removal enhancement and greenhouse gas emissions reduction of constructed wetland by optimizing its redox environment through manganese oxide addition.

Manganese oxide (MnOx) is receiving increased interest in the nutrient removal of constructed wetlands (CWs); however, its service effectiveness for simultaneous greenhouse gas (GHG) emissions reduction is still vague. In this study, three vertical flow CWs, i.e., volcanics (CCW), manganese sand uniformly mixing with volcanics (Mn-CW) and MnOx doped volcanics (MnV-CW), were constructed to investigate the underlying mechanisms of MnOx on nutrient removal enhancement and greenhouse gas (GHG) emissions reduction. The results showed that the MnOx doped volcanics optimized the oxidation-reduction potential surrounding the substrate (-164.0 ∼ +141.1 mv), and resulted in the lowest GHG emissions (CO2-equivalent) from MnV-CW, 16.8-36.5 % lower than that of Mn-CW and CCW. This was mainly ascribed to mitigation of N2O produced during the NO3--N reduction process, according to results of 15N stable isotope labeling. Analysis of the microbial community structure revealed that due to the optimized redox conditions through chemical doping of MnOx on volcanics, the abundance of microbe involved in denitrification and Mn-oxidizing process in the MnV-CW was significantly increased at genus level, which led to a higher Mn cycling efficiency between biogenic MnOx and Mn2+, and enhanced denitrification efficiency and N2O emission reduction. This study would help to understand and provide a preferable reference for future applications for manganese-based CW.

Synergetic effect of pyrrhotite and zero-valent iron on Hg(â ¡) removal in constructed wetland: Mechanisms of electron transfer and microbial reaction.

Effective removal of mercury (Hg) from wastewater is significant due to its high toxicity, especially methylmercury (MeHg). Reducing of Hg(II) to Hg(0) in constructed wetlands (CWs) using iron-based materials is an effective strategy for preventing the formation of MeHg. However, the surface passivation of zero-valent iron (ZVI) limits its application. Herein, synergetic ZVI and pyrrhotite were utilized to enhance Hg removal in CWs. Results indicated that the removal of total Hg, dissolved Hg, and particulate Hg in CWs with ZVI and pyrrhotite were improved by 21.68 ± 0.76 %, 13.02 ± 0.88 %, and 22.27 ± 0.76 % compared to that with single ZVI or pyrrhotite. Pyrrhotite increased the surface corrosion of ZVI, thereby facilitating the process of iron reduction. The redox of iron promoted the generation of EPS, which could provide electrons for Hg(II) reduction. The sulfur also participates in electron transfer by driving the methylation of Hg and provides sulfides to form FeS-Hg complexes and HgS precipitation. The abundance of key enzymes that involved in iron reduction and Hg transformation was enhanced with the addition of ZVI and pyrrhotite. The synergetic of pyrrhotite and ZVI enhances the removal of Hg in CW, offering a promising technology for high-efficiency treatment of Hg.

Overlooked dissemination risks of antimicrobial resistance through green tide proliferation.

Green tides, particularly those induced by Enteromorpha, pose significant environmental challenges, exacerbated by climate change, coastal eutrophication, and other anthropogenic impacts. More concerningly, these blooms may influence the spread of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) within ecosystems. However, the manner in which Enteromorpha blooms affect the distribution and spread of antimicrobial resistance (AMR) remains uncertain. This study investigated ARG profiles, dynamic composition, and associated health risks within the Enteromorpha phycosphere and surrounding seawater in typical bays (Jiaozhou, Aoshan, and Lingshan) in the South Yellow Sea. The Enteromorpha phycosphere exhibited significantly higher ARG abundance (p < 0.05) but lower diversity compared to the surrounding seawater. Source-tracking and metagenomic analyses revealed that the phycosphere was the main contributor to the resistome of surrounding seawater. Moreover, resistant pathogens, especially ESKAPE pathogens, with horizontal gene transfer (HGT) potential, were more abundant in the phycosphere than in the surrounding seawater. The phycosphere released high-risk ARGs to the surrounding seawater during Enteromorpha blooms, posing serious health and ecological AMR risks in marine environments. This study highlights the significant role of Enteromorpha blooms in ARG spread and associated risks, urging a reassessment of AMR burden from a public health perspective.

Enhanced removal of phenanthrene and nutrients in wetland sediment with metallic biochar: Performance and mechanisms.

Polycyclic aromatic hydrocarbon (PAHs) are persistent organic pollutants and pose high risk in aquatic environment. The utilization of biochar is a strategy for PAHs-contaminated remediation but is challenging due to the adsorption saturation and reoccurrence of PAHs desorbed back into water. In this study, iron (Fe) and manganese (Mn) were provided as electron acceptors for biochar modification to enhance anaerobic biodegradation of phenanthrene (Phe). Results revealed that, the Mn(Ⅳ) and Fe(Ⅲ) modification improved the removal of Phe by 24.2% and 31.4% than that of biochar, respectively. Additionally, nitrate removal was improved by 19.5% with Fe(Ⅲ) amendment. The Mn-and Fe-biochar decreased Phe contents by 8.7% and 17.4% in sediment, 10.3% and 13.8% in biochar than that of biochar. Much higher DOC contents were observed with Mn- and Fe-biochar, which provided bioavailable carbon source for microbes and contributed to microbial degradation of Phe. The greater degree of humification, higher proportions of humic and fulvic acid like components in metallic biochar participated in electron transport and further enhancing the degradation of PAHs. Microbial analysis proved the high abundance of Phe-degrading bacteria (e.g. PAH-RHDα, Flavobacterium and Vibrio), nitrogen removal microbes (e.g. amoA, nxrA, and nir), Fe and Mn bioreduction or oxidation (e. g. Bacillus, Thermomonas, Deferribacter) with metallic biochar. Based on the results, the Fe and Mn modification, especially Fe-modified biochar provided well performance for PAHs removal in aquatic sediment.