RESUMEN
Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria.
Asunto(s)
Compuestos de Amonio/metabolismo , Bacterias/genética , Consorcios Microbianos/genética , Ácido Úrico/metabolismo , Bacterias/metabolismo , Biodegradación Ambiental , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Jordania , Mar Mediterráneo , Petróleo/metabolismo , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/genética , ARN Ribosómico 16S/metabolismo , Análisis de Secuencia de ADNRESUMEN
Helicobacter pylori is a bacterial pathogen which can lead to several human gastric diseases. Here we describe the genome sequences of three strains isolated from atrophic gastritis and gastric ulcers patients in China. The data will permit genomic characterization of traits that may contribute to various gastric diseases.
Asunto(s)
Gastritis Atrófica/microbiología , Infecciones por Helicobacter/microbiología , Helicobacter pylori/genética , Úlcera Gástrica/microbiología , China/epidemiología , Gastritis Atrófica/epidemiología , Infecciones por Helicobacter/epidemiología , Helicobacter pylori/clasificación , Humanos , Datos de Secuencia Molecular , Úlcera Gástrica/epidemiologíaRESUMEN
Pelagibacterium halotolerans B2(T) is a marine halotolerant bacterium that was isolated from a seawater sample collected from the East China Sea. Here, we present the complete genome sequence of the type strain P. halotolerans B2(T), which consists of one chromosome (3,944,837 bp; 61.4% G+C content) and one plasmid (4,050 bp; 56.1% G+C content). This is the first complete genome of a member of the Pelagibacterium genus.
Asunto(s)
Alphaproteobacteria/genética , Genoma Bacteriano , Datos de Secuencia Molecular , Océanos y Mares , Agua de Mar/microbiologíaRESUMEN
Most of the species in the family Planctomycetaceae are of interest for their eukaryotic-like cell structures and characteristics of resistance to extreme environments. Here, we report draft genome sequences of three aquatic parasitic species of this family, Singulisphaera acidiphila (DSM 18658T), Schlesneria paludicola (DSM 18645T), and Zavarzinella formosa (DSM 19928T).
Asunto(s)
Genoma Bacteriano , Planctomycetales/genética , Regulación Bacteriana de la Expresión Génica/fisiología , Datos de Secuencia Molecular , Planctomycetales/clasificación , Especificidad de la EspecieRESUMEN
The draft genome sequence (4,398,155 bp, with 65.35% G+C content) of Halobiforma lacisalsi AJ5, an extremely halophilic archaeon isolated from a salt lake, is reported here. This is the first genome report for a species of the Halobiforma genus.
Asunto(s)
Proteínas Arqueales/genética , Bacteriorodopsinas/genética , Genoma Arqueal , Halobacteriaceae/genética , Lagos/microbiología , Cloruro de Sodio/metabolismo , Proteínas Arqueales/metabolismo , Bacteriorodopsinas/metabolismo , Secuencia de Bases , Halobacteriaceae/clasificación , Halobacteriaceae/aislamiento & purificación , Halobacteriaceae/metabolismo , Lagos/química , Datos de Secuencia MolecularRESUMEN
Two of the largest crude oil-polluted areas in the world are the semi-enclosed Mediterranean and Red Seas, but the effect of chronic pollution remains incompletely understood on a large scale. We compared the influence of environmental and geographical constraints and anthropogenic forces (hydrocarbon input) on bacterial communities in eight geographically separated oil-polluted sites along the coastlines of the Mediterranean and Red Seas. The differences in community compositions and their biodegradation potential were primarily associated (P < 0.05) with both temperature and chemical diversity. Furthermore, we observed a link between temperature and chemical and biological diversity that was stronger in chronically polluted sites than in pristine ones where accidental oil spills occurred. We propose that low temperature increases bacterial richness while decreasing catabolic diversity and that chronic pollution promotes catabolic diversification. Our results further suggest that the bacterial populations in chronically polluted sites may respond more promptly in degrading petroleum after accidental oil spills.