RESUMEN
Osteosarcoma (OS) has become one of the most common primary malignant tumors in the children and adolescents with a poor prognosis owing to its high malignant and metastatic potential. Although increasing evidence indicates that miR-451 could inhibit the growth and metastasis of OS, its effect on angiogenesis in OS is still very poor. What is more, the mechanism by which miR-451 affects the OS has not been fully elucidated. In the present study, miR-451 was reduced in human osteosarcoma tissues compared with the adjacent bone tissues, and the introduction of miR-451 dramatically inhibited the growth, migration and angiogenesis in OS. Additionally, it was suggested that IL 6R is a direct target gene of miR-451. Silencing of IL 6R suppressed the growth, migration and angiogenesis of OS, which was consistent with the effect of overexpression of miR-451. In conclusion, our data demonstrate that miR-451 may function as a potential suppressor of tumor growth, migration and angiogenesis in OS via down-regulating IL 6R, suggesting a promising therapeutic avenue for managing OS.
Asunto(s)
Neoplasias Óseas/genética , Huesos/patología , MicroARNs/genética , Neovascularización Patológica/genética , Osteosarcoma/genética , Receptores de Interleucina-6/genética , Animales , Secuencia de Bases , Neoplasias Óseas/irrigación sanguínea , Neoplasias Óseas/patología , Huesos/irrigación sanguínea , Huesos/metabolismo , Línea Celular Tumoral , Movimiento Celular , Proliferación Celular , Regulación hacia Abajo , Femenino , Regulación Neoplásica de la Expresión Génica , Células Endoteliales de la Vena Umbilical Humana , Humanos , Ratones Endogámicos BALB C , Ratones Desnudos , Neovascularización Patológica/patología , Osteosarcoma/irrigación sanguínea , Osteosarcoma/patologíaRESUMEN
Both bone marrow mesenchymal stromal cells (BMSCs) and adipose-derived mesenchymal stromal cells (ADSCs) are good sources for tissue engineering. To maximize therapeutic efficacy of MSCs, an appropriate source of MSCs should be selected according to their own inherent characteristics for future clinical application. Hence, this study was conducted to compare proliferative, differential and antiapoptosis abilities of both MSCs derived from exercised and sedentary rats under normal and hypoxia/serum deprivation conditions (H/SD). Our results showed that exercise may enhance proliferative ability and decrease adipogenic ability of BMSCs and ADSCs. However, positive effect of exercise on osteogenesis was only observed for BMSCs in either environment. Little effect was observed on the antiapoptotic ability of both MSC types. It was also suggested that biological characteristics of both types were partly changed. It is therefore believed that BMSCs derived from exercised rat on early passage may be a good cell source for bone tissue engineering.
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Diferenciación Celular/fisiología , Proliferación Celular/fisiología , Células Madre Mesenquimatosas/fisiología , Osteogénesis/fisiología , Condicionamiento Físico Animal/fisiología , Tejido Adiposo/citología , Animales , Apoptosis , Células de la Médula Ósea/fisiología , Células Cultivadas , Citometría de Flujo , Masculino , Ratas , Reacción en Cadena en Tiempo Real de la Polimerasa , Transducción de SeñalRESUMEN
The advent of artificial intelligence (AI), particularly ChatGPT/GPT-4, has led to advancements in various fields, including healthcare. This study explores the prospective role of ChatGPT/GPT-4 in various facets of spinal surgical practice, especially in supporting spinal surgeons during the perioperative management of endoscopic spinal surgery for patients with lumbar disc herniation. The AI-driven chatbot can facilitate communication between spinal surgeons, patients, and their relatives, streamline the collection and analysis of patient data, and contribute to the surgical planning process. Furthermore, ChatGPT/GPT-4 may enhance intraoperative support by providing real-time surgical navigation information and physiological parameter monitoring, as well as aiding in postoperative rehabilitation guidance. However, the appropriate and supervised use of ChatGPT/GPT-4 is essential, considering the potential risks associated with data security and privacy. The study concludes that ChatGPT/GPT-4 can serve as a valuable lighthouse for spinal surgeons if used correctly and responsibly.
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Cirujanos , Cirugía Asistida por Computador , Humanos , Inteligencia Artificial , Procedimientos Neuroquirúrgicos , Programas InformáticosRESUMEN
Biomedical engineering is a relatively young interdisciplinary field based on engineering, biology, and medicine. Of note, the rapid progress of artificial intelligence (AI)-based technologies has made a significant impact on the biomedical engineering field, and continuously bring innovations and breakthroughs. Recently, ChatGPT, an AI chatbot developed by OpenAI company, has gained tremendous attention due to its powerful natural language generation and understanding ability. In this study, we explored potential of GPT-4 in the eight branches of biomedical engineering including medical imaging, medical devices, bioinformatics, biomaterials, biomechanics, gene and cell engineering, tissue engineering, and neural engineering. Our results show that the application of GPT-4 will bring new opportunities for the development of this field.
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Inteligencia Artificial , Ingeniería Biomédica , Bioingeniería , Ingeniería de Tejidos , Materiales BiocompatiblesRESUMEN
The emergence of artificial intelligence (AI) offers unprecedented opportunities for joint arthroplasty surgery. Notably, on 14th March, 2023, the OpenAI company officially launched its latest version GPT-4, which once again become the focus of discussion on social media. Although more than 200 articles have reported the potential role of ChatGPT/GPT-4 in various areas, there are no studies that discussed the potential of GPT-4 as an AI-powered virtual assistant for surgeons specialized in joint arthroplasty. In this study, we summarized the five major roles of GPT-4 including scientific research, disease diagnosis, treatment options, preoperative planning, intraoperative support, and postoperative rehabilitation for arthroplasty doctors. Of note, in parallel to enjoy AI dividend, it is also necessary to pay attention to protect the data from misuse with ethical considerations in place.
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Inteligencia Artificial , Cirujanos , Humanos , ArtroplastiaRESUMEN
In the era of big data, generative artificial intelligence (AI) models are currently in a boom. The Chatbot Generative Pre-trained Transformer (ChatGPT), a large language model (LLM) developed by OpenAI (San Francisco, CA), is a type of AI software that could generate text based on the input it receives. In this study, in order to explore how ChatGPT could give reflections and suggestions about the sudden outbreak of Mpox in 2022 from the AI dimensions, our group talked with ChatGPT with several questions about Mpox. We hope this talk could enrich our knowledge on Mpox from the new AI dimensions and also explore the possibility of human and AI fight shoulder to shoulder for prevention and containment of the potential epidemics or pandemics in future.
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Inteligencia Artificial , Mpox , Humanos , Programas Informáticos , Brotes de Enfermedades , Suministros de Energía EléctricaRESUMEN
Sports medicine, an essential branch of orthopedics, focuses on preserving, restoring, improving, and rebuilding the function of the human motor system. As a thriving interdisciplinary field, sports medicine attracts not only the interest of the orthopedic community, but also artificial intelligence (AI). In this study, our team summarized the potential applications of GPT-4 in sports medicine including diagnostic imaging, exercise prescription, medical supervision, surgery treatment, sports nutrition, and science research. In our opinion, it is impossible that GPT-4 could make sports physicians obsolete. Instead, it could become an indispensable scientific assistant for sport doctors in future.
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Procedimientos Ortopédicos , Médicos , Medicina Deportiva , Deportes , Humanos , Inteligencia ArtificialRESUMEN
Over the past month, a new AI model called Chatbot Generative Pre-trained Transformer (ChatGPT), has received enormous attention in the media and scientific communities due to its ability to process and respond to commands in a humanistic fashion. As reported, five days after its launch, the number of registered users of ChatGPT exceeded one million, and its monthly active users had exceeded 100 million two months later, making it the most rapidly growing consumer application in history. The advent of ChatGPT has further brought about new ideas and challenges in the realm of infectious disease. In view of this, in order to evaluate the potential use of ChatGPT in clinical practice and scientific research of infectious disease, we conducted a brief online survey by using the publicly available ChatGPT webpage. Also, the present study also talks about the relevant social and ethical issues related to this program.
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Inteligencia Artificial , Enfermedades Transmisibles , Humanos , Programas Informáticos , Suministros de Energía EléctricaRESUMEN
Background: This study investigated the association between post-traumatic chronic osteomyelitis (COM) and peripheral leukocyte telomere length (PLTL) and explored factors associated with PLTL in COM. Methods: A total of 56 patients with post-traumatic COM of the extremity and 62 healthy control subjects were recruited. The PLTL was measured by real-time PCR. Binary logistic regression analysis was used to identify factors in correlation with telomere length. Sex, age, white blood cell (WBC) count, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and infection duration were included as independent variables in the logistic regression model. Results: Post-traumatic COM patients had significantly shorter PLTLs (5.39 ± 0.40) than healthy control subjects (5.69 ± 0.46; p < 0.001). Binary logistic regression analysis showed that PLTL had a statistically significant association with age (B = -0.072; p = 0.013) and CRP (B = -0.061; p = 0.033). The logistic regression model was statistically significant and explained 31.4% (Nagelkerke R2) of the change in telomere length and correctly classified 69.6% of the cases. Conclusions: Patients with post-traumatic COM have shorter PLTLs than healthy subjects. The PLTL erosion of post-traumatic COM was partially explained by age and CRP.
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Leucocitos/patología , Osteomielitis/genética , Osteomielitis/patología , Telómero/genética , Proteína C-Reactiva , Humanos , Recuento de LeucocitosRESUMEN
INTRODUCTION: The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. MATERIAL AND METHODS: The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. Thereafter, BMSCs were subjected to 10% elongation using a Flexcell strain unit, combined with 20 IU/ml EPO. The proliferation of BMSCs was detected by Cell Counting Kit-8, colony formation assay, and cell cycle assay; meanwhile, the mRNA expression levels of Ets-1, C-myc, Ccnd1, and C-fos were detected by reverse transcription and real-time quantitative PCR (qPCR). The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. The role of the extracellular signal-regulated kinases 1/2 (ERK1/2) in the osteogenesis of BMSCs stimulated by mechanical stretch combined with 20 IU/ml EPO was examined by Western blot. RESULTS: Our results showed that effects of EPO on BMSCs included a dose-response relationship, with the 20 IU/ml EPO yielding the largest. Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. The increase in ALP, mineral deposition, and osteoblastic genes induced by the mechanical stretch-EPO combination was inhibited by U0126, an ERK1/2 inhibitor. CONCLUSION: EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. The underlying mechanism may be related to the activation of the ERK1/2 signaling pathway.
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It is understood that mechanical loading may affect tendon properties. However, how different mechanical loading conditions may affect tendons remains unknown. The present study aimed to investigate the effect of treadmill running at various intensities on rat Achilles tendon. A total of 18 male Wistar rats were randomly assigned to one of three groups: Control (CON), medium-intensity running (MIR), and high-intensity running (HIR). Following 8 weeks of treadmill running protocols, all Achilles tendons were harvested for histological observation and gene expression analysis. Significant morphological changes were observed with regular and large diameter collagen fibrils in the MIR group, whereas irregular and small diameter collagen fibrils were observed in the HIR group. Collagen type I was significantly upregulated in the MIR group compared with the CON group, and downregulated in the HIR group compared with the CON or MIR groups (P<0.05). However, collagen type III was significantly upregulated in the HIR group in comparison with the CON or MIR groups (P<0.05). Furthermore, the expression of matrix metallopeptidase-13 was significantly increased in the MIR and HIR groups compared with the CON group (P<0.05). The expression of tissue inhibitor of metalloproteinases-1 was increased in the MIR group compared with the CON group, but decreased in the HIR group compared with the CON and MIR groups (P<0.05). Additionally, decorin expression was significantly higher in the MIR group compared with the CON group, and significantly decreased in the HIR group compared with the CON or MIR groups (P<0.05). A converse pattern of changes in biglycan expression was identified among the three groups. Aggrecan expression was significantly higher in the HIR group compared with the CON or MIR groups (P<0.05). These findings indicated that moderate exercise may induce increased collagen synthesis and organize regular and large collagen fibers, thus benefiting the Achilles tendon. However, overuse during exercise may result in collagen degradation and disturbance, which predisposes individuals to injury.
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Decorin is widely understood to affect collagen fibrillogenesis. However, little is understood about its response to various mechanical loading conditions. In the present study, 36 Wistar rats were randomly divided into control (CON), moderate treadmill running (MTR) and strenuous treadmill running (STR) groups. Animals in the MTR and STR groups were subjected to a 4 or 8week treadmill running protocol. Subsequently, all Achilles tendons were harvested to perform histological and biochemical analyses. Decorin expression was markedly increased in the MTR group compared with the CON group at 4 and 8 weeks. Conversely, decorin expression was markedly decreased in the STR group compared with the CON and MTR group at 4 and 8 weeks. Furthermore, between the two time points, decorin expression levels were significantly increased in the MTR group, whereas they were markedly decreased in the STR group. These results suggested that MTR exercise may induce increased decorin expression via a balance of MMP2 and TIMP2, improving tendon structure and function. However, STR exercise may result in degradation of decorin due to an imbalance of MMP2 and TIMP2, with a bias to MMP2, resulting in a predisposition to tendinopathy.
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Decorina/farmacología , Condicionamiento Físico Animal , Esfuerzo Físico/efectos de los fármacos , Animales , Conducta Animal , Biomarcadores , Inmunohistoquímica , Masculino , RatasRESUMEN
Previous studies have shown that MiR-451 plays an important role in human osteosarcoma carcinogenesis, but the underlying mechanism by which MiR-451 affects the osteosarcoma has not been fully understood. This study intends to uncover the mechanism by which MiR-451 functions as a tumor suppressor. The expression of MiR-451 in osteosarcoma tissues and osteosarcoma cell lines was monitored by real-time PCR. The proliferation ability was examined by MTT and cell cycle assay. The migration and apoptosis of cells were monitored by migration assay and flow cytometry, respectively. Moreover, the angiogenesis of HUVEC cells transfected with MiR-451 mimics was examined by tube formation assay. The effect of MiR-451 on MIF was determined by luciferase assays and Western blot assay. The results showed that MiR-451 expression level was significantly reduced in the osteosarcoma compared with normal bone tissues. Overexpression of MiR-451 significantly attenuated the proliferation and migration, and induced the apoptosis of osteosarcoma cells. Furthermore, the angiogenesis of HUVEC cells transfected with MiR-451 mimics was assayed and the decreased angiogenic ability was detected compared to the controls. Finally, we demonstrated that MiR-451 overexpression inhibited the malignant behavior of osteosarcoma by downregulating MIF. These findings suggest that MiR-451 may act as a tumor suppressor in osteosarcoma. MiR-451 inhibited cell proliferation, migration and angiogenesis and promoted apoptosis of human osteosarcoma cells, at least partially, by inhibiting the expression of MIF. MiR-451/MIF may be a novel therapeutic target in treatment of osteosarcoma.
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Apoptosis/genética , Neoplasias Óseas/genética , Movimiento Celular/genética , Proliferación Celular/genética , Oxidorreductasas Intramoleculares/genética , Factores Inhibidores de la Migración de Macrófagos/genética , MicroARNs/genética , Osteosarcoma/genética , Neoplasias Óseas/patología , Ciclo Celular/genética , Línea Celular , Línea Celular Tumoral , Regulación hacia Abajo/genética , Regulación Neoplásica de la Expresión Génica/genética , Genes Supresores de Tumor/fisiología , Células Endoteliales de la Vena Umbilical Humana , Humanos , Invasividad Neoplásica/genética , Neovascularización Patológica/genética , Neovascularización Patológica/patología , Osteosarcoma/patologíaRESUMEN
This study was aimed to investigate the spatial and temporal changes of subchondral bone and its overlying articular cartilage in rats following knee immobilization. A total of 36 male Wistar rats (11-13 months old) were assigned randomly and evenly into 3 groups. For each group, knee joints in 6 rats were immobilized unilaterally for 1, 4, or 8 weeks, respectively, while the remaining rats were allowed free activity and served as external control groups. For each animal, femurs at both sides were dissected after sacrificed. The distal part of femur was examined by micro-CT. Subsequently, femoral condyles were collected for further histological observation and analysis. For articular cartilage, significant changes were observed only at 4 and 8 weeks of immobilization. The thickness of articular cartilage and chondrocytes numbers decreased with time. However, significant changes in subchondral bone were defined by micro-CT following immobilization in a time-dependent manner. Immobilization led to a thinner and more porous subchondral bone plate, as well as a reduction in trabecular thickness and separation with a more rod-like architecture. Changes in subchondral bone occurred earlier than in articular cartilage. More importantly, immobilization-induced changes in subchondral bone may contribute, at least partially, to changes in its overlying articular cartilage.