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1.
Proc Natl Acad Sci U S A ; 117(26): 14936-14947, 2020 06 30.
Artículo en Inglés | MEDLINE | ID: mdl-32541055

RESUMEN

Mre11 and Rad50 (M/R) proteins are part of an evolutionarily conserved macromolecular apparatus that maintains genomic integrity through repair pathways. Prior structural studies have revealed that this apparatus is extremely dynamic, displaying flexibility in the long coiled-coil regions of Rad50, a member of the structural maintenance of chromosome (SMC) superfamily of ATPases. However, many details of the mechanics of M/R chromosomal manipulation during DNA-repair events remain unclear. Here, we investigate the properties of the thermostable M/R complex from the archaeon Sulfolobus acidocaldarius using atomic force microscopy (AFM) to understand how this macromolecular machinery orchestrates DNA repair. While previous studies have observed canonical interactions between the globular domains of M/R and DNA, we observe transient interactions between DNA substrates and the Rad50 coiled coils. Fast-scan AFM videos (at 1-2 frames per second) of M/R complexes reveal that these interactions result in manipulation and translocation of the DNA substrates. Our study also shows dramatic and unprecedented ATP-dependent DNA unwinding events by the M/R complex, which extend hundreds of base pairs in length. Supported by molecular dynamic simulations, we propose a model for M/R recognition at DNA breaks in which the Rad50 coiled coils aid movement along DNA substrates until a DNA end is encountered, after which the DNA unwinding activity potentiates the downstream homologous recombination (HR)-mediated DNA repair.


Asunto(s)
Proteínas Arqueales/metabolismo , Endodesoxirribonucleasas/metabolismo , Exodesoxirribonucleasas/metabolismo , Proteína Homóloga de MRE11/metabolismo , Sulfolobus acidocaldarius/genética , Proteínas Arqueales/química , Proteínas Arqueales/genética , ADN de Archaea/química , ADN de Archaea/genética , ADN de Archaea/metabolismo , Endodesoxirribonucleasas/química , Endodesoxirribonucleasas/genética , Exodesoxirribonucleasas/química , Exodesoxirribonucleasas/genética , Proteína Homóloga de MRE11/química , Proteína Homóloga de MRE11/genética , Microscopía de Fuerza Atómica , Unión Proteica , Sulfolobus acidocaldarius/química , Sulfolobus acidocaldarius/enzimología , Sulfolobus acidocaldarius/metabolismo
2.
Biochem Soc Trans ; 48(6): 2359-2376, 2020 12 18.
Artículo en Inglés | MEDLINE | ID: mdl-33300987

RESUMEN

The bacterial SbcC/SbcD DNA repair proteins were identified over a quarter of a century ago. Following the subsequent identification of the homologous Mre11/Rad50 complex in the eukaryotes and archaea, it has become clear that this conserved chromosomal processing machinery is central to DNA repair pathways and the maintenance of genomic stability in all forms of life. A number of experimental studies have explored this intriguing genome surveillance machinery, yielding significant insights and providing conceptual advances towards our understanding of how this complex operates to mediate DNA repair. However, the inherent complexity and dynamic nature of this chromosome-manipulating machinery continue to obfuscate experimental interrogations, and details regarding the precise mechanisms that underpin the critical repair events remain unanswered. This review will summarize our current understanding of the dramatic structural changes that occur in Mre11/Rad50 complex to mediate chromosomal tethering and accomplish the associated DNA processing events. In addition, undetermined mechanistic aspects of the DNA enzymatic pathways driven by this vital yet enigmatic chromosomal surveillance and repair apparatus will be discussed. In particular, novel and putative models of DNA damage recognition will be considered and comparisons will be made between the modes of action of the Rad50 protein and other related ATPases of the overarching SMC superfamily.


Asunto(s)
Proteínas Bacterianas/química , Roturas del ADN de Doble Cadena , Reparación del ADN , Desoxirribonucleasas/química , Proteínas de Escherichia coli/química , Exonucleasas/química , Ácido Anhídrido Hidrolasas/metabolismo , Adenosina Trifosfatasas/química , Adenosina Trifosfato/química , Proteínas Arqueales/metabolismo , Proteínas Bacterianas/metabolismo , Ciclo Celular , ADN/química , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Desoxirribonucleasas/metabolismo , Endodesoxirribonucleasas/química , Endodesoxirribonucleasas/metabolismo , Proteínas de Escherichia coli/metabolismo , Exodesoxirribonucleasas/metabolismo , Exonucleasas/metabolismo , Humanos , Hidrólisis , Proteína Homóloga de MRE11/metabolismo , Mutación , Unión Proteica , Conformación Proteica , Zinc/química
3.
Angew Chem Int Ed Engl ; 59(31): 12698-12702, 2020 07 27.
Artículo en Inglés | MEDLINE | ID: mdl-32297692

RESUMEN

We report the use of DNA origami nanostructures, functionalized with aptamers, as a vehicle for delivering the antibacterial enzyme lysozyme in a specific and efficient manner. We test the system against Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) targets. We use direct stochastic optical reconstruction microscopy (dSTORM) and atomic force microscopy (AFM) to characterize the DNA origami nanostructures and structured illumination microscopy (SIM) to assess the binding of the origami to the bacteria. We show that treatment with lysozyme-functionalized origami slows bacterial growth more effectively than treatment with free lysozyme. Our study introduces DNA origami as a tool in the fight against antibiotic resistance, and our results demonstrate the specificity and efficiency of the nanostructure as a drug delivery vehicle.


Asunto(s)
Antibacterianos/farmacología , ADN/química , Portadores de Fármacos/química , Muramidasa/farmacología , Nanoestructuras/química , Animales , Antibacterianos/química , Aptámeros de Nucleótidos/química , Aptámeros de Nucleótidos/toxicidad , Bacillus subtilis/química , Bacillus subtilis/efectos de los fármacos , Células COS , Chlorocebus aethiops , ADN/toxicidad , Portadores de Fármacos/toxicidad , Enzimas Inmovilizadas/química , Enzimas Inmovilizadas/farmacología , Escherichia coli/química , Escherichia coli/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Muramidasa/química , Nanoestructuras/toxicidad , Conformación de Ácido Nucleico
4.
J Biol Chem ; 291(3): 1014-27, 2016 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-26546679

RESUMEN

The majority of biosynthetic secretory proteins initiate their journey through the endomembrane system from specific subdomains of the endoplasmic reticulum. At these locations, coated transport carriers are generated, with the Sar1 GTPase playing a critical role in membrane bending, recruitment of coat components, and nascent vesicle formation. How these events are appropriately coordinated remains poorly understood. Here, we demonstrate that Sar1 acts as the curvature-sensing component of the COPII coat complex and highlight the ability of Sar1 to bind more avidly to membranes of high curvature. Additionally, using an atomic force microscopy-based approach, we further show that the intrinsic GTPase activity of Sar1 is necessary for remodeling lipid bilayers. Consistent with this idea, Sar1-mediated membrane remodeling is dramatically accelerated in the presence of its guanine nucleotide-activating protein (GAP), Sec23-Sec24, and blocked upon addition of guanosine-5'-[(ß,γ)-imido]triphosphate, a poorly hydrolysable analog of GTP. Our results also indicate that Sar1 GTPase activity is stimulated by membranes that exhibit elevated curvature, potentially enabling Sar1 membrane scission activity to be spatially restricted to highly bent membranes that are characteristic of a bud neck. Taken together, our data support a stepwise model in which the amino-terminal amphipathic helix of GTP-bound Sar1 stably penetrates the endoplasmic reticulum membrane, promoting local membrane deformation. As membrane bending increases, Sar1 membrane binding is elevated, ultimately culminating in GTP hydrolysis, which may destabilize the bilayer sufficiently to facilitate membrane fission.


Asunto(s)
Vesículas Cubiertas por Proteínas de Revestimiento/metabolismo , Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/fisiología , Retículo Endoplásmico/metabolismo , GTP Fosfohidrolasas/metabolismo , Guanosina Trifosfato/metabolismo , Modelos Biológicos , Proteínas de Unión al GTP Monoméricas/metabolismo , Sustitución de Aminoácidos , Animales , Vesículas Cubiertas por Proteínas de Revestimiento/efectos de los fármacos , Vesículas Cubiertas por Proteínas de Revestimiento/ultraestructura , Caenorhabditis elegans/efectos de los fármacos , Caenorhabditis elegans/enzimología , Caenorhabditis elegans/ultraestructura , Proteínas de Caenorhabditis elegans/antagonistas & inhibidores , Proteínas de Caenorhabditis elegans/química , Proteínas de Caenorhabditis elegans/genética , Retículo Endoplásmico/ultraestructura , Inhibidores Enzimáticos/farmacología , GTP Fosfohidrolasas/antagonistas & inhibidores , GTP Fosfohidrolasas/química , GTP Fosfohidrolasas/genética , Proteínas Activadoras de GTPasa/antagonistas & inhibidores , Proteínas Activadoras de GTPasa/genética , Proteínas Activadoras de GTPasa/metabolismo , Guanilil Imidodifosfato/farmacología , Humanos , Membrana Dobles de Lípidos/química , Membrana Dobles de Lípidos/metabolismo , Microdominios de Membrana/efectos de los fármacos , Microdominios de Membrana/metabolismo , Microdominios de Membrana/ultraestructura , Microscopía de Fuerza Atómica , Proteínas de Unión al GTP Monoméricas/antagonistas & inhibidores , Proteínas de Unión al GTP Monoméricas/química , Proteínas de Unión al GTP Monoméricas/genética , Mutación , Forma de los Orgánulos/efectos de los fármacos , Interferencia de ARN , Proteínas Tirosina Quinasas Receptoras/genética , Proteínas Tirosina Quinasas Receptoras/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Proteínas de Transporte Vesicular/antagonistas & inhibidores , Proteínas de Transporte Vesicular/genética , Proteínas de Transporte Vesicular/metabolismo , Proteínas Activadoras de ras GTPasa/genética , Proteínas Activadoras de ras GTPasa/metabolismo
5.
J Biol Chem ; 288(2): 778-84, 2013 Jan 11.
Artículo en Inglés | MEDLINE | ID: mdl-23223336

RESUMEN

NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity. Here, we used atomic force microscopy (AFM) imaging to visualize activation-induced structural changes in the GluN1/GluN2A NMDA receptor reconstituted into a lipid bilayer. In the absence of agonist, AFM imaging revealed two populations of particles with heights above the bilayer surface of 8.6 and 3.4 nm. The taller, but not the shorter, particles could be specifically decorated by an anti-GluN1 antibody, which recognizes the S2 segment of the agonist-binding domain, indicating that the two populations represent the extracellular and intracellular regions of the receptor, respectively. In the presence of glycine and glutamate, there was a reduction in the height of the extracellular region to 7.3 nm. In contrast, the height of the intracellular domain was unaffected. Fast-scan AFM imaging combined with UV photolysis of caged glutamate permitted the detection of a rapid reduction in the height of individual NMDA receptors. The reduction in height did not occur in the absence of the co-agonist glycine or in the presence of the selective NMDA receptor antagonist D(-)-2-amino-5-phosphonopentanoic acid, indicating that the observed structural change was caused by receptor activation. These results represent the first demonstration of an activation-induced effect on the structure of the NMDA receptor at the single-molecule level. A change in receptor size following activation could have important functional implications, in particular by affecting interactions between the NMDA receptor and its extracellular synaptic partners.


Asunto(s)
Microscopía de Fuerza Atómica/métodos , Receptores de N-Metil-D-Aspartato/metabolismo , Células HEK293 , Humanos , Conformación Proteica , Receptores de N-Metil-D-Aspartato/química
6.
Nucleic Acids Res ; 39(11): 4525-31, 2011 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-21310716

RESUMEN

Much insight into the interactions of DNA and enzymes has been obtained using a number of single-molecule techniques. However, recent results generated using two of these techniques-atomic force microscopy (AFM) and magnetic tweezers (MT)-have produced apparently contradictory results when applied to the action of the ATP-dependent type III restriction endonucleases on DNA. The AFM images show extensive looping of the DNA brought about by the existence of multiple DNA binding sites on each enzyme and enzyme dimerisation. The MT experiments show no evidence for looping being a requirement for DNA cleavage, but instead support a diffusive sliding of the enzyme on the DNA until an enzyme-enzyme collision occurs, leading to cleavage. Not only do these two methods appear to disagree, but also the models derived from them have difficulty explaining some ensemble biochemical results on DNA cleavage. In this 'Survey and Summary', we describe several different models put forward for the action of type III restriction enzymes and their inadequacies. We also attempt to reconcile the different models and indicate areas for further experimentation to elucidate the mechanism of these enzymes.


Asunto(s)
ADN/metabolismo , Desoxirribonucleasas de Localización Especificada Tipo III/metabolismo , Modelos Biológicos , ADN/química , Microscopía de Fuerza Atómica , Transporte de Proteínas
7.
Biochemistry ; 51(2): 578-85, 2012 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-22225525

RESUMEN

G-Quadruplexes are nucleic acid secondary structures consisting of a planar arrangement of four guanine residues. Potential G-quadruplex-forming sequences are widely distributed throughout the genome. Significantly, they are present in telomeres and are enriched in gene promoters and first introns, raising the possibility that perturbation of G-quadruplex stability might have therapeutic potential, for example in the treatment of cancer. Ligands that interact selectively with G-quadruplexes include both proteins and small molecules, although the interactions between ligands and their G-quadruplex targets have been monitored using indirect methods. In addition, the G-quadruplex targets have often been short DNA fragments. Here, we have used atomic force microscopy imaging to examine directly at the single-molecule level the interaction of ligands with G-quadruplexes generated during transcription of a plasmid containing a G-rich insert. We show that the structures produced during transcription are decorated specifically by the single-chain antibody HF1 and by the nuclear protein PARP-1, both of which are known to recognize G-quadruplexes. Our results provide clear structural evidence of G-quadruplex formation in a transcription-dependent case and demonstrate directly how small-molecule stabilizers and destabilizers can manipulate these structures in a biochemically functional system.


Asunto(s)
G-Cuádruplex/efectos de los fármacos , Microscopía de Fuerza Atómica , Plásmidos/química , Plásmidos/metabolismo , Aminoquinolinas/farmacología , Animales , Secuencia de Bases , Humanos , Ligandos , Ratones , Ácidos Picolínicos/farmacología , Plásmidos/genética , Poli(ADP-Ribosa) Polimerasa-1 , Poli(ADP-Ribosa) Polimerasas/metabolismo , Anticuerpos de Cadena Única/metabolismo , Transcripción Genética/efectos de los fármacos
8.
Biophys J ; 101(12): 2992-8, 2011 Dec 21.
Artículo en Inglés | MEDLINE | ID: mdl-22208198

RESUMEN

Many DNA regulatory factors require communication between distantly separated DNA sites for their activity. The type IIF restriction enzyme SfiI is often used as a model system of site communication. Here, we used fast-scanning atomic force microscopy to monitor the DNA cleavage process with SfiI and the changes in the single SfiI-DNA complex in the presence of either Mg²âº or Ca²âº at a scan rate of 1-2 fps. The increased time resolution allowed us to visualize the concerted cleavage of the protein at two cognate sites. The four termini generated by the cleavage were released in a multistep manner. The high temporal resolution enabled us to visualize the translocation of a DNA strand on a looped complex and intersegmental transfer of the SfiI protein in which swapping of the site is performed without protein dissociation. On the basis of our results, we propose that the SfiI tetramer can remain bound to one of the sites even after cleavage, allowing the other site on the DNA molecule to fill the empty DNA-binding cleft by combining a one-dimensional diffusion-mediated sliding and a segment transfer mechanism.


Asunto(s)
ADN/química , ADN/ultraestructura , Desoxirribonucleasas de Localización Especificada Tipo II/química , Desoxirribonucleasas de Localización Especificada Tipo II/ultraestructura , Modelos Moleculares , Sitios de Unión , Simulación por Computador , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/ultraestructura , Activación Enzimática , Cinética , Modelos Químicos , Unión Proteica , Especificidad por Sustrato
9.
Nucleic Acids Res ; 37(18): 6269-75, 2009 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-19696072

RESUMEN

The formation of G-quadruplexes in G-rich regions of DNA is believed to affect DNA transcription and replication. However, it is currently unclear how this formation occurs in the presence of a complementary strand. We have used atomic force microscopy (AFM) to image stable RNA/DNA hybrid loops generated by transcription of the plasmid pPH600, which contains a 604-bp fragment of the murine immunoglobulin Sgamma3 switch region. We show that the non-RNA-containing portion folds into G-quadruplexes, consistent with computational predictions. We also show that hybrid formation prevents further transcription from occurring, implying a regulatory role. After in vitro transcription, almost all (93%) of the plasmids had an asymmetric loop, a large asymmetric blob or a spur-like projection at the appropriate position on the DNA contour. The loops disappeared following treatment of the transcribed plasmid with RNase H, which removes mRNA hybridized with the template strand. Replacement of K+ in the transcription buffer with either Na+ or Li+ caused a reduction in the percentage of plasmids containing loops, blobs or spurs, consistent with the known effects of monovalent cations on G-quadruplex stability. The minimal sample preparation required for AFM imaging has permitted direct observation of the structural changes resulting from G-quadruplex formation.


Asunto(s)
ADN/química , G-Cuádruplex , ADN/ultraestructura , Microscopía de Fuerza Atómica , ARN/química , Transcripción Genética
10.
Nucleic Acids Res ; 37(6): 2053-63, 2009 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-19223329

RESUMEN

Atomic force microscopy (AFM) allows the study of single protein-DNA interactions such as those observed with the Type I Restriction-Modification systems. The mechanisms employed by these systems are complicated and understanding them has proved problematic. It has been known for years that these enzymes translocate DNA during the restriction reaction, but more recent AFM work suggested that the archetypal EcoKI protein went through an additional dimerization stage before the onset of translocation. The results presented here extend earlier findings confirming the dimerization. Dimerization is particularly common if the DNA molecule contains two EcoKI recognition sites. DNA loops with dimers at their apex form if the DNA is sufficiently long, and also form in the presence of ATPgammaS, a non-hydrolysable analogue of the ATP required for translocation, indicating that the looping is on the reaction pathway of the enzyme. Visualization of specific DNA loops in the protein-DNA constructs was achieved by improved sample preparation and analysis techniques. The reported dimerization and looping mechanism is unlikely to be exclusive to EcoKI, and offers greater insight into the detailed functioning of this and other higher order assemblies of proteins operating by bringing distant sites on DNA into close proximity via DNA looping.


Asunto(s)
Enzimas de Restricción del ADN/ultraestructura , ADN/ultraestructura , Adenosina Trifosfato/análogos & derivados , Adenosina Trifosfato/metabolismo , Sitios de Unión , ADN/química , ADN/metabolismo , Enzimas de Restricción del ADN/metabolismo , Interpretación Estadística de Datos , Dimerización , Microscopía de Fuerza Atómica , Unión Proteica , Multimerización de Proteína , Metiltransferasa de ADN de Sitio Específico (Adenina Especifica)/metabolismo , Metiltransferasa de ADN de Sitio Específico (Adenina Especifica)/ultraestructura
11.
Biophys J ; 98(7): 1182-91, 2010 Apr 07.
Artículo en Inglés | MEDLINE | ID: mdl-20371317

RESUMEN

The sigma-1 receptor is a widely expressed protein that interacts with a variety of ion channels, including the acid-sensing ion channel (ASIC) 1a. Here we used atomic force microscopy to determine the architecture of the ASIC1a/sigma-1 receptor complex. When isolated His(8)-tagged ASIC1a was imaged in complex with anti-His(6) antibodies, the angle between pairs of bound antibodies was 135 degrees , consistent with the known trimeric structure of the channel. When ASIC1a was coexpressed with FLAG/His(6)-tagged sigma-1 receptor, ASIC1a became decorated with small particles, and pairs of these particles bound at an angle of 131 degrees . When these complexes were incubated with anti-FLAG antibodies, pairs of antibodies bound at an angle of 134 degrees , confirming that the small particles were sigma-1 receptors. Of interest, we found that the sigma-1 receptor ligand haloperidol caused an approximately 50% reduction in ASIC1a/sigma-receptor binding, suggesting a way in which sigma-1 ligands might modulate channel properties. For the first time, to our knowledge, we have resolved the structure of a complex between the sigma-1 receptor and a target ion channel, and demonstrated that the stoichiometry of the interaction is 1 sigma-1 receptor/1 ASIC1a subunit.


Asunto(s)
Proteínas del Tejido Nervioso/química , Proteínas del Tejido Nervioso/metabolismo , Receptores sigma/metabolismo , Canales de Sodio/química , Canales de Sodio/metabolismo , Canales Iónicos Sensibles al Ácido , Línea Celular , Haloperidol/química , Histidina/química , Humanos , Iones , Ligandos , Microscopía de Fuerza Atómica/métodos , Microscopía Fluorescente/métodos , Modelos Biológicos , Transfección , Receptor Sigma-1
12.
Biophys J ; 99(8): 2550-8, 2010 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-20959096

RESUMEN

Synaptotagmin I is the major Ca²(+) sensor for membrane fusion during neurotransmitter release. The cytoplasmic domain of synaptotagmin consists of two C2 domains, C2A and C2B. On binding Ca²(+), the tips of the two C2 domains rapidly and synchronously penetrate lipid bilayers. We investigated the forces of interaction between synaptotagmin and lipid bilayers using single-molecule force spectroscopy. Glutathione-S-transferase-tagged proteins were attached to an atomic force microscope cantilever via a glutathione-derivatized polyethylene glycol linker. With wild-type C2AB, the force profile for a bilayer containing phosphatidylserine had both Ca²(+)-dependent and Ca²(+)-independent components. No force was detected when the bilayer lacked phosphatidylserine, even in the presence of Ca²(+). The binding characteristics of C2A and C2B indicated that the two C2 domains cooperate in binding synaptotagmin to the bilayer, and that the relatively weak Ca²(+)-independent force depends only on C2A. When the lysine residues K189-192 and K326, 327 were mutated to alanine, the strong Ca²(+)-dependent binding interaction was either absent or greatly reduced. We conclude that synaptotagmin binds to the bilayer via C2A even in absence of Ca²(+), and also that positively charged regions of both C2A and C2B are essential for the strong Ca²(+)-dependent binding of synaptotagmin to the bilayer.


Asunto(s)
Membrana Dobles de Lípidos/metabolismo , Microscopía de Fuerza Atómica , Sinaptotagminas/metabolismo , Calcio/metabolismo , Membrana Celular/química , Membrana Celular/metabolismo , Citoplasma/metabolismo , Membrana Dobles de Lípidos/química , Mutación , Unión Proteica , Estructura Terciaria de Proteína , Sinaptotagminas/química , Sinaptotagminas/genética
13.
Open Biol ; 9(10): 190094, 2019 10 31.
Artículo en Inglés | MEDLINE | ID: mdl-31615332

RESUMEN

The synaptonemal complex (SC) keeps homologous chromosomes in close alignment during meiotic recombination. A hallmark of the SC is the presence of its constituent protein SYCP3 on the chromosome axis. During SC assembly, SYCP3 is deposited on both axes of the homologue pair, forming axial elements that fuse into the lateral element (LE) in the tripartite structure of the mature SC. We have used cryo-electron tomography and atomic force microscopy to study the mechanism of assembly and DNA binding of the SYCP3 fibre. We find that the three-dimensional architecture of the fibre is built on a highly irregular arrangement of SYCP3 molecules displaying very limited local geometry. Interaction between SYCP3 molecules is driven by the intrinsically disordered tails of the protein, with no contact between the helical cores, resulting in a flexible fibre assembly. We demonstrate that the SYCP3 fibre can engage in extensive interactions with DNA, indicative of an efficient mechanism for incorporation of DNA within the fibre. Our findings suggest that SYCP3 deposition on the chromosome axis might take place by polymerization into a fibre that is fastened to the chromosome surface via DNA binding.


Asunto(s)
Proteínas de Ciclo Celular/química , Cromosomas/ultraestructura , Proteínas de Unión al ADN/química , Sitios de Unión , Proteínas de Ciclo Celular/metabolismo , Cromosomas/química , Cromosomas/metabolismo , ADN/química , ADN/metabolismo , Proteínas de Unión al ADN/metabolismo , Humanos , Unión Proteica , Multimerización de Proteína
14.
Mol Pharmacol ; 73(3): 960-7, 2008 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-18079275

RESUMEN

The GABA(A) receptor is a chloride-selective ligand-gated ion channel of the Cys-loop superfamily. The receptor consists of five subunits arranged pseudosymmetrically around a central pore. The predominant form of the receptor in the brain contains alpha(1)-, beta(2)-, and gamma(2)-subunits in the arrangement alphabetaalphagammabeta, counter-clockwise around the pore. GABA(A) receptors containing delta-instead of gamma-subunits, although a minor component of the total receptor population, have interesting properties, such as an extrasynaptic location, high sensitivity to GABA, and potential association with conditions such as epilepsy. They are therefore attractive targets for drug development. Here we addressed the subunit arrangement within the alpha(4)beta(3)delta form of the receptor. Different epitope tags were engineered onto the three subunits, and complexes between receptors and anti-epitope antibodies were imaged by atomic force microscopy. Determination of the numbers of receptors doubly decorated by each of the three antibodies revealed a subunit stoichiometry of 2alpha:2beta:1delta. The distributions of angles between pairs of antibodies against the alpha- and beta-subunits both had peaks at around 144 degrees , indicating that these pairs of subunits were nonadjacent. Decoration of the receptor with ligands that bind to the extracellular domain (i.e., the lectin concanavalin A and an antibody that recognizes the beta-subunit N-terminal sequence) showed that the receptor preferentially binds to the mica extracellular face down. Given this orientation, the geometry of complexes of receptors with both an antibody against the delta-subunit and Fab fragments against the alpha-subunits indicates a predominant subunit arrangement of alphabetaalphadeltabeta, counter-clockwise around the pore when viewed from the extracellular space.


Asunto(s)
Microscopía de Fuerza Atómica , Subunidades de Proteína/química , Receptores de GABA-A/química , Receptores de GABA-A/ultraestructura , Animales , Anticuerpos Monoclonales/metabolismo , Complejo Antígeno-Anticuerpo/química , Antígenos Transformadores de Poliomavirus/genética , Antígenos Transformadores de Poliomavirus/metabolismo , Línea Celular , Células Clonales , ADN Complementario/química , Técnica del Anticuerpo Fluorescente Indirecta , Histidina/química , Peroxidasa de Rábano Silvestre/metabolismo , Humanos , Riñón/citología , Ratas , Receptores de GABA-A/aislamiento & purificación , Receptores de GABA-A/metabolismo , Virus 40 de los Simios/fisiología , Solubilidad , Transfección
15.
Biochem Biophys Res Commun ; 372(4): 752-5, 2008 Aug 08.
Artículo en Inglés | MEDLINE | ID: mdl-18514062

RESUMEN

There has been confusion about the subunit stoichiometry of the degenerin family of ion channels. Recently, a crystal structure of acid-sensing ion channel (ASIC) 1a revealed that it assembles as a trimer. Here, we used atomic force microscopy (AFM) to image unprocessed ASIC1a bound to mica. We detected a mixture of subunit monomers, dimers and trimers. In some cases, triple-subunit clusters were clearly visible, confirming the trimeric structure of the channel, and indicating that the trimer sometimes disaggregated after adhesion to the mica surface. This AFM-based technique will now enable us to determine the subunit arrangement within heteromeric ASICs.


Asunto(s)
Microscopía de Fuerza Atómica , Proteínas del Tejido Nervioso/química , Proteínas del Tejido Nervioso/ultraestructura , Canales de Sodio/química , Canales de Sodio/ultraestructura , Canales Iónicos Sensibles al Ácido , Silicatos de Aluminio/química , Humanos , Subunidades de Proteína/química
16.
Micron ; 39(2): 101-10, 2008.
Artículo en Inglés | MEDLINE | ID: mdl-17296302

RESUMEN

Fast neurotransmission involves the operation of ionotropic receptors, which are multi-subunit proteins that respond to activation by opening an integral ion channel. Examples of such channels include the GABA(A) receptor, the 5-HT(3) receptor and the P2X receptor for ATP. These receptors contain more than one type of subunit, although the exact subunit stoichiometry and arrangement around the receptor rosette is often unknown. We are using atomic force microscopy (AFM) of purified receptors to address these issues. Measurement of the molecular volume of the receptor permits the determination of the number of subunits that it contains. Furthermore, analysis of the geometry of complexes between receptors and subunit-specific antibodies reveals the subunit arrangement. Our AFM-based approach has so far been dependent on manual data processing, which is both time-consuming and prone to operator bias. In this study, we set out to develop a novel method capable of automatic segmentation and quantitative analysis of both single receptor particles and receptor-antibody complexes. The method was validated using images of wild type and mutant forms of the P2X(6) receptor. We suggest that the automated method will greatly facilitate further progress in the use of AFM for the determination of receptor and multi-protein architecture.


Asunto(s)
Complejo Antígeno-Anticuerpo/ultraestructura , Microscopía de Fuerza Atómica/métodos , Receptores Purinérgicos P2/química , Receptores Purinérgicos P2/ultraestructura , Animales , Complejo Antígeno-Anticuerpo/química , Automatización , Línea Celular , Complejos Multiproteicos/química , Complejos Multiproteicos/ultraestructura , Subunidades de Proteína/química
17.
J Mol Biol ; 345(4): 745-58, 2005 Jan 28.
Artículo en Inglés | MEDLINE | ID: mdl-15588823

RESUMEN

Atomic force microscopy (AFM) has been used to examine the conformational effects of echinomycin, a DNA bis-intercalating antibiotic, on linear and circular DNA. Four different 398 bp DNA fragments were synthesized, comprising a combination of normal and/or modified bases including 2,6-diaminopurine and inosine (which are the corresponding analogues of adenine and guanosine in which the 2-amino group that is crucial for echinomycin binding has been added or removed, respectively). Analysis of AFM images provided contour lengths, which were used as a direct measure of bis-intercalation. About 66 echinomycin molecules are able to bind to each fragment, corresponding to a site size of six base-pairs. The presence of base-modified nucleotides affects DNA conformation, as determined by the helical rise per base-pair. At the same time, the values obtained for the dissociation constant correlate with the types of preferred binding site available among the different DNA fragments; echinomycin binds to TpD sites much more tightly than to CpG sites. The structural perturbations induced when echinomycin binds to closed circular duplex pBR322 DNA were also investigated and a method for quantification of the structural changes is presented. In the presence of increasing echinomycin concentration, the plasmid can be seen to proceed through a series of transitions in which its supercoiling decreases, relaxes, and then increases.


Asunto(s)
2-Aminopurina/análogos & derivados , ADN/metabolismo , ADN/ultraestructura , Equinomicina/metabolismo , Equinomicina/farmacología , Microscopía de Fuerza Atómica , 2-Aminopurina/farmacología , ADN/química , ADN Circular/química , ADN Circular/metabolismo , ADN Circular/ultraestructura , Equinomicina/química , Enlace de Hidrógeno/efectos de los fármacos , Sustancias Intercalantes/química , Sustancias Intercalantes/metabolismo , Sustancias Intercalantes/farmacología , Ligandos , Conformación de Ácido Nucleico/efectos de los fármacos , Plásmidos/química , Plásmidos/metabolismo , Plásmidos/ultraestructura
18.
Nucleic Acids Res ; 30(13): 2980-6, 2002 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-12087184

RESUMEN

Atomic force microscopy (AFM) has been used to examine perturbations in the tertiary structure of DNA induced by the binding of ditercalinium, a DNA bis-intercalator with strong anti-tumour properties. We report AFM images of plasmid DNA of both circular and linearised forms showing a difference in the formation of supercoils and plectonemic coils caused at least in part by alterations in the superhelical stress upon bis-intercalation. A further investigation of the effects of drug binding performed with 292 bp mixed-sequence DNA fragments, and using increment in contour length as a reliable measure of intercalation, revealed saturation occurring at a point where sufficient drug was present to interact with every other available binding site. Moment analysis based on the distribution of angles between segments along single DNA molecules showed that at this level of bis-intercalation, the apparent persistence length of the molecules was 91.7 +/- 5.7 nm, approximately twice as long as that of naked DNA. We conclude that images of single molecules generated using AFM provide a valuable supplement to solution-based techniques for evaluation of physical properties of biological macromolecules.


Asunto(s)
Carbazoles/química , ADN/química , Microscopía de Fuerza Atómica/métodos , ADN/genética , ADN/ultraestructura , Etidio/química , Conformación de Ácido Nucleico , Plásmidos/química , Plásmidos/genética
19.
Biochem J ; 381(Pt 3): 911-7, 2004 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-15128269

RESUMEN

The Helicobacter pylori vacuolating toxin VacA causes several effects on mammalian cells in vitro, including intracellular vacuolation, formation of pores in the plasma membrane and apoptosis. When added to cells, VacA becomes associated with detergent-resistant membranes, indicating that it binds preferentially to lipid rafts. In the present study, we have used atomic force microscopy to examine directly the association of VacA with lipid domains in supported lipid bilayers. VacA did not bind to lipid bilayers at pH 7.6. In contrast, at pH 4.0, VacA associated with the bilayers in the form of 26-nm oligomeric complexes. VacA bound to bilayers produced from either brain lipids or SM (sphingomyelin) plus cholesterol, each of which lacked detectable lipid domains. Bilayers composed of DOPC (dioleoylphosphatidylcholine), SM and cholesterol contained clearly visible raft-like domains, and VacA preferentially associated with these rafts. VacA bound poorly to raft-like domains in DOPC/SM bilayers, indicating that cholesterol is required for efficient association of VacA with lipid domains. When PS (phosphatidylserine), an anionic phospholipid that does not partition significantly into rafts, was added to the mixture of DOPC, SM and cholesterol, VacA was excluded from the rafts, indicating that it binds more avidly to PS than to the raft components. A typical plasma membrane exhibits pronounced lipid asymmetry, with SM enriched in the outer leaflet and PS in the inner leaflet. Therefore it is probable that the association of VacA with rafts in DOPC/SM/cholesterol bilayers represents a useful model for understanding the interactions of VacA with membranes in vivo.


Asunto(s)
Proteínas Bacterianas/metabolismo , Microdominios de Membrana/química , Microscopía de Fuerza Atómica/métodos , Colesterol/química , Concentración de Iones de Hidrógeno , Membrana Dobles de Lípidos/química , Membrana Dobles de Lípidos/metabolismo , Liposomas/química , Microdominios de Membrana/metabolismo , Fosfatidilcolinas/química , Polímeros/química , Polímeros/metabolismo , Esfingomielinas/química
20.
Expert Opin Drug Discov ; 10(3): 221-9, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25549544

RESUMEN

INTRODUCTION: Atomic force microscopy (AFM) is a scanning probe technique that has been in use in biology to generate sub-nanometre resolution images in near-physiological environments for over 20 years. Most AFM work uses instruments that take several minutes to generate each image but instruments that can produce real-time images have recently become available and there is now a reasonable body of work published on this technique. The importance of this high-speed AFM is that dynamic events of individual macromolecules can be studied. AREAS COVERED: This review focuses on specific examples that demonstrate the potential of the technique. It covers four areas in which high-speed AFM has been used to elucidate mechanisms that are either unstudied or not clearly understood. These areas are: protein-protein interactions; DNA-protein interactions; quantification of biological processes; the use of DNA origami scaffolds as nanostructures to build and study dynamic molecular events. EXPERT OPINION: High-speed AFM shares advantages and disadvantages with conventional AFM, but it compares well in quality of data generated and in ease of use with other currently available techniques of high-resolution biological imaging. As the instruments become more widespread, the value of high-speed AFM and its potential to complement other techniques in molecular and cell biology should become more appreciated.


Asunto(s)
Microscopía de Fuerza Atómica/métodos , Nanoestructuras/química , Proteínas/metabolismo , ADN/metabolismo , Humanos , Simulación de Dinámica Molecular
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