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Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are important metabolizing enzymes functioning by adding a sugar moiety to a small lipophilic substrate molecule and play critical roles in drug/toxin metabolism for all realms of life. In this study, the silkworm Bombyx mori UGT33D1 gene was characterized in detail. UGT33D1 was found localized in the endoplasmic reticulum (ER) compartment just like other animal UGTs and was mainly expressed in the silkworm midgut. We first reported that UGT33D1 was important to BmNPV infection, as silencing UGT33D1 inhibited the BmNPV infection in silkworm BmN cells, while overexpressing the gene promoted viral infection. The molecular pathways regulated by UGT33D1 were analysed via transcriptome sequencing upon UGT33D1 knockdown, highlighting the important role of the gene in maintaining a balanced oxidoreductive state of the organism. In addition, proteins that physically interact with UGT33D1 were identified through immunoprecipitation and mass spectrometry analysis, which includes tubulin, elongation factor, certain ribosomal proteins, histone proteins and zinc finger proteins that had been previously reported for human UGT-interacting proteins. This study provided preliminary but important functional information on UGT33D1 and is hoped to trigger deeper investigations into silkworm UGTs and their functional mechanisms.
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Microalgae are the preferred species for producing astaxanthin because they pose a low toxicity risk than chemical synthesis. Astaxanthin has multiple health benefits and is being used in: medicines, nutraceuticals, cosmetics, and functional foods. Haematococcus pluvialis is a model microalga for astaxanthin biosynthesis; however, its natural astaxanthin content is low. Therefore, it is necessary to develop methods to improve the biosynthesis of astaxanthin to meet industrial demands, making its commercialization cost-effective. Several strategies related to cultivation conditions are employed to enhance the biosynthesis of astaxanthin in H. pluvialis. However, the mechanism of its regulation by transcription factors is unknown. For the first time, this study critically reviewed the studies on identifying transcription factors, progress in H. pluvialis genetic transformation, and use of phytohormones that increase the gene expression related to astaxanthin biosynthesis. In addition, we propose future approaches, including (i) Cloning and characterization of transcription factors, (ii) Transcriptional engineering through overexpression of positive regulators or downregulation/silencing of negative regulators, (iii) Gene editing for enrichment or deletion of transcription factors binding sites, (iv) Hormonal modulation of transcription factors. This review provides considerable knowledge about the molecular regulation of astaxanthin biosynthesis and the existing research gap. Besides, it provides the basis for transcription factors mediated metabolic engineering of astaxanthin biosynthesis in H. pluvialis.
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In this work, a highly sensitive ratiometric homogeneous electroanalysis (HEA) strategy of cadmium(II) (Cd2+) was proposed via a Cd2+-controlled redox reaction and Ru(bpy)32+ (Ru(II)) release from a smart metal-organic framework (MOF) nanomaterial. For achieving this purpose, Ru(II) was entrapped ingeniously into the pores of an MOF material (UiO-66-NH2) and subsequently gated by the double-strand hybrids of a Cd2+-aptamer (Apt) and its complementary sequences (CP) to form a novel smart nanomaterial (denoted as Ru@UiO-66-NH2); meanwhile, Fe(III) was selected as an additional probe present in electrolyte to facilitate the Ru(II) redox reaction: Fe(III) + Ru(II) â Fe(II) + Ru(III). Owing to the strong binding effect of the Cd2+ target to the specific Apt, the Apt-CP hybridization at Ru@UiO-66-NH2 would be destroyed in the presence of Cd2+, and the related Apt was further induced away from the smart nanomaterial, leading to the opening of the gate and release of Ru(II). Meanwhile, the released Ru(II) was quickly oxidized chemically by Fe(III) to Ru(III). On the basis of the generated Ru(III) and consumed Fe(III), the ratio of the reduction currents between Ru(III) and Fe(III) exhibits an enhancement and it is dependent on the level of Cd2+; thus, a novel HEA strategy of Cd2+ was then designed. Under the optimal conditions, the HEA sensor shows a wide linearity ranging from 10.0 pM to 500.0 nM, and the achieved detection limit of Cd2+ is 3.3 pM. The as-designed ratiometric HEA strategy not only offers a unique idea to realize a simple and sensitive assay for Cd2+ but also possesses significant potential as an effective tool to be introduced for other target analysis just via altering the specific Apt.
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Parkinson's disease (PD) is a chronic and progressive movement disorder that is characterized by the loss of dopaminergic neurons in the brain. Animal models of PD have become very popular in the past two decades to understand the etiology, pathology, and molecular and cellular pathways associated with PD. In this study, we report the first neurotoxin-induced silkworm model for PD by chronic feeding with 6-hydroxydopamine (6-OHDA) and explore the possible molecular mechanisms associated with PD using proteomic and targeted metabolomic approaches. Although silkworm is phylogenetically distant from humans and rats, 6-OHDA treatment produced similar PD phenotypes, including motor dysfunction, dopaminergic neuron degeneration, and decreased levels of dopamine. Major neurotransmitters in the silkworm head tissue were profiled, revealing key molecules implicating neurodegenerative disorder. Proteomics analysis revealed a major downregulation of nearly 50 structural proteins constituting cuticles and microfilaments, indicating mechanical damage in the silkworm tissues. The results suggest that 6-OHDA treatment could induce PD-like symptoms in silkworms and activate similar proteomic and metabolic pathways to those in rats or higher animals. This study demonstrates the feasibility and value of the silkworm-based PD model, which may provide important clues for understanding the molecular and cellular mechanisms underlying PD. The mass spectrometry raw files have been deposited to iProx via the project ID IPX0004206000.
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Bombyx , Enfermedad de Parkinson , Animales , Bombyx/genética , Bombyx/metabolismo , Modelos Animales de Enfermedad , Dopamina/metabolismo , Neuronas Dopaminérgicas , Humanos , Oxidopamina/farmacología , Enfermedad de Parkinson/genética , Enfermedad de Parkinson/metabolismo , Proteómica , RatasRESUMEN
Elucidating the mechanism of infection of Bombyx mori nuclear polyhedrosis virus (BmNPV) and host antiviral response remains a major scientific task in sericulture. Virus invasion causes a series of antiviral immune responses in the host, and successful infection leads to massive changes in the host's physiological and biochemical state. Current research mainly focuses on silkworm genes and proteins associated with viral infection and resistance, but little is known regarding the host metabolic pathways that the virus utilizes for optimal replication. In this work, key metabolites involved in viral infection were identified, including trehalose, riboflavin, tryptophan, tyrosine, and phenylalanine. The genes associated with metabolite biosynthesis and catabolism were analyzed, and their expression levels were found to be largely consistent with their respective metabolite levels before and after viral treatment in both strains. The screened metabolites were further investigated for their roles in viral replication using exogenous metabolite addition into the culture medium. The results showed that tryptophan effectively inhibited BmNPV replication, while glutamine promoted viral replication in a dose-dependent manner. Trehalose and riboflavin exhibited a complex effect on BmNPV replication. This study outlines the critical metabolites and metabolic pathways required for BmNPV to proliferate and infect the host, indicting the potential of metabolite-based treatment for viral inhibition.
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Bombyx , Nucleopoliedrovirus , Virosis , Animales , Antivirales/metabolismo , Proteínas de Insectos/metabolismo , Redes y Vías Metabólicas , Nucleopoliedrovirus/fisiología , Riboflavina/metabolismo , Riboflavina/farmacología , Trehalosa/metabolismo , Trehalosa/farmacología , Triptófano/metabolismoRESUMEN
Lipids have been recently proposed as key molecules for virus entry and egress, and lipid biosynthesis and signaling were reported necessary for some viruses during replication and infection. The silkworm Bombyx mori is an important economic insect and a model organism, but its lipid profiles have not been systematically investigated. Most silkworm strains are susceptible to the B. mori nuclear polyhedrovirus (BmNPV), a baculovirus that causes serious loss to the sericulture industry. Previously, our lab has screened a natural mutant of B. mori that is highly resistant to BmNPV. In this study, a comprehensive lipidomic analysis by ultrahigh pressure liquid chromatography-mass spectrometry (UPLC-MS) was carried out on the BmNPV-susceptible strain 306 and resistant strain NB (data deposited in MetaboLight MTBLS2142). Comparisons of the lipid profiles between the two strains reveal that phosphosphingolipids, diacylglycerolipids, ceramides, and quinones were present at notably higher levels in the susceptible strain, while lysophosphocholines were found at a higher level in the resistant strain. BmNPV administration changed the lipid profiles in both strains, revealing key lipids involved in virus infection and immune response. Some key enzymes in the lipid biosynthesis pathway were analyzed for their activities in the two silkworm strains and their virus-administered counterparts, underlining the relation among lipid biosynthesis, viral resistance, and immune response in the host.
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Bombyx , Nucleopoliedrovirus , Virosis , Animales , Cromatografía Liquida , Lipidómica , Espectrometría de Masas en TándemRESUMEN
Most of the oleaginous microorganisms cannot assimilate xylose in the presence of glucose, which is the major bottleneck in the bioconversion of lignocellulose to biodiesel. Our present study revealed that overexpression of xylose isomerase (XI) gene xylA or xylulokinase (XK) gene xks1 increased the xylose consumption by 25 to 37% and enhanced the lipid content by 8 to 28% during co-fermentation of glucose and xylose. In xylA overexpressing strain Mc-XI, the activity of XI was 1.8-fold higher and the mRNA level of xylA at 24 h and 48 h was 11- and 13-fold higher than that of the control, respectively. In xks1 overexpressing strain Mc-XK, the mRNA level of xks1 was 4- to 11-fold of that of the control strain and the highest XK activity of 950 nmol min-1 mg-1 at 72 h which was 2-fold higher than that of the control. Additionally, expression of a translational fusion of xylA and xks1 further enhanced the xylose utilization rate by 45%. Our results indicated that overexpression of xylA and/or xks1 is a promising strategy to improve the xylose and glucose co-utilization, alleviate the glucose repression, and produce lipid from lignocellulosic biomass in the oleaginous fungus M. circinelloides. KEY POINTS: ⢠Overexpressing xylA or xks1 increased the xylose consumption and the lipid content. ⢠The xylose isomerase activity and the xylA mRNA level were enhanced in strain Mc-XI. ⢠Co-expression of xylA and xks1 further enhanced the xylose utilization rate by 45%.
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Glucosa , Xilosa , Isomerasas Aldosa-Cetosa , Fermentación , Mucor/genética , Fosfotransferasas (Aceptor de Grupo Alcohol)RESUMEN
The effects of PMF (5-7 T, 5-30 pulses) on enzyme activity, pH, titratable acidity, soluble solids, color, ascorbic acid, total phenols and antioxidant activity (DPPH radical scavenging activity) of cloudy apple juice were evaluated. PMF inhibited activities of polyphenoloxidase (PPO), peroxidase (POD) and pectinmethylesterase (PME), but PPO was more sensitive to PMF than POD and PME. At the intensity of 6 T with 15 pulses, PPO and POD both exhibited the lowest residual activity (53.22 and 92.96%), while PME showed the lowest residual activity (83.01%) at 7 T with 30 pulses. No significant effect on soluble solids was found under all processing parameters, whereas significant decreases of ascorbic acid were observed at the intensity of 7 T with 5-30 pulses. PMF did not change pH, titratable acidity, color, total phenols and DPPH radical scavenging activity severely. These results suggest PMF can be a potential technology for enzymatic inactivation in apple juice with high retention of quality.
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Microalgae have been widely used for treatment of swine wastewater. However, the research on combined treatment of refractory pollutants ammonia nitrogen, Cu (II) and antibiotics from swine wastewater was still scattered. This study, the growth and removal efficiency of NH4Cl, CuSO4, tetracycline, norfloxacin and sulfadimidine with selected Scenedsmus sp. was investigated by biofilm attached culture. The results showed that low concentration of ammonia nitrogen had little effect on algae growth. The highest biomass productivity was 6.2 g/(m2d) at the concentration of NH4Cl of 50.0 mg/L, which was similar to that of a standard growth medium BG 11. Cu (II) concentration of 1.0 mg/L could accelerate the growth of Scenedsmus sp., and the highest biomass was 57.2 g/m2 in 8 days. Moreover, the highest biomass mean values was 59.5 g/m2, 57.1 g/m2, and 58.1 g/m2, respectively, when tetracycline concentration was 20.0 mg/L, norfloxacin concentration was 100.0 mg/L and sulfadimidine concentration was 10.0 mg/L. The removal efficiency of ammonia nitrogen, copper, tetracycline, norfloxacin and sulfadimidine with Scenedsmus sp. at their optimal initial concentration by biofilm attached culture was 85.2%, 64.6%, 74.6%,71.2%, and 62.3%, respectively. This study provides a theoretical basis for the purification of refractory substances from swine wastewater.
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Contaminantes Ambientales , Microalgas , Scenedesmus , Animales , Biodegradación Ambiental , Biopelículas , Biomasa , Nitrógeno , Fósforo , Porcinos , Aguas ResidualesRESUMEN
OBJECTIVES: To demonstrate the effectiveness of a novel two-stage system coupling hydrolytic acidification with algal microcosms for the treatment of acrylonitrile butadiene styrene (ABS) resin-manufacturing wastewater. RESULTS: After hydrolytic acidification, the BOD5/COD ratio increased from 0.22 to 0.56, showing improved biodegradability of the wastewater. Coupled with hydrolytic acidification, the algal microcosms showed excellent capability of in-depth removal of COD, NH3-N and phosphorus with removal rates 83, 100, and 89%, respectively, and aromatic pollutants, including benzene, were almost completely removed. The biomass concentration of Chlorella sp. increased from 5 × 106 to 2.1 × 107 cells/ml after wastewater treatment. CONCLUSIONS: This two-stage coupling system achieved deep cleaning of the benzene-containing petrochemical wastewater while producing greater algae biomass resources at low cost.
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Resinas Acrílicas/toxicidad , Biodegradación Ambiental , Butadienos/toxicidad , Chlorella/química , Poliestirenos/toxicidad , Aguas Residuales/química , Ácidos/química , Resinas Acrílicas/química , Biomasa , Butadienos/química , Chlorella/metabolismo , Humanos , Hidrólisis , Poliestirenos/química , Eliminación de Residuos Líquidos/métodos , Aguas Residuales/toxicidad , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/toxicidadRESUMEN
Agricultural soils contaminated with heavy metals (HMs) impose a threat to the environmental and to human health. Amendment with biochar could be an eco-friendly and cost-effective option to decrease HMs in contaminated soil. This paper reviews the application of biochar as a soil amendment to immobilise HMs in contaminated soil. We discuss the technologies of its preparation, their specific properties, and effect on the bioavailability of HMs. Biochar stabilises HMs in contaminated soil, enhance the overall quality of the contaminated soil, and significantly reduce HM uptake by plants, making it an option in soil remediation for HM contamination. Biochar enhances the physical (e.g. bulk density, soil structure, water holding capacity), chemical (e.g. cation exchange capacity, pH, nutrient availability, ion exchange, complexes), and biological properties (e.g. microbial abundance, enzymatic activities) of contaminated soil. Biochar also enhances soil fertility, improves plant growth, and reduces the plant availability of HMs. Various field studies have shown that biochar application reduces the bioavailability of HMs from contaminated soil while increasing crop yield. The review highlights the positive effects of biochar by reducing HM bioavailability in contaminated soils. Future work is recommended to ensure that biochars offer a safe and sustainable solution to remediate soils contaminated with HMs.
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Carbón Orgánico , Metales Pesados , Contaminantes del Suelo , Humanos , Suelo/química , Metales Pesados/análisis , Agricultura , PlantasRESUMEN
Urban areas remarkably affect global public health due to their emissions of greenhouse gases and poor air quality. Although urban areas only cover 2% of the Earth's surface, they are responsible for 80% of greenhouse gas emissions. Dense buildings limit vegetation, leading to increased air pollution and disruption of the local and regional carbon cycle. The substitution of urban gray roofs with microalgal green roofs has the potential to improve the carbon cycle by sequestering CO2 from the atmosphere. Microalgae can fix 15-50 times more CO2 than other types of vegetation. Advanced microalgal-based green roof technology may significantly accelerate the reduction of atmospheric CO2 in a more effective way. Microalgal green roofs also enhance air quality, oxygen production, acoustic isolation, sunlight absorption, and biomass production. This endeavor yields the advantage of simultaneously generating protein, lipids, vitamins, and a spectrum of valuable bioactive compounds, including astaxanthin, carotenoids, polysaccharides, and phycocyanin, thus contributing to a green economy. The primary focus of the current work is on analyzing the ecological advantages and CO2 bio-fixation efficiency attained through microalgal cultivation on urban rooftops. This study also briefly examines the idea of green roofs, clarifies the ecological benefits associated with them, discusses the practice of growing microalgae on rooftops, identifies the difficulties involved, and the positive aspects of this novel strategy.
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Gases de Efecto Invernadero , Microalgas , Fotobiorreactores , Dióxido de Carbono/metabolismo , Microalgas/metabolismo , Microclima , BiomasaRESUMEN
BACKGROUND: Catechol (CC), a prevalent phenolic compound, is a byproduct in various agricultural, chemical, and industrial processes. CC detection is crucial for safeguarding water quality and plays a pivotal role in enhancing the overall quality of life of individuals. Electrochemical biosensors exhibit rapid responses, have small sizes, and can be used for real-time monitoring. Therefore, the development of a fast and sensitive electrochemical biosensor for CC detection is crucial. RESULT: In this study, a laccase-based electrochemical biosensor for detection of CC is successfully developed using Fe3O4 nanoparticles as medium and optimized by applying a magnetic field. This research proposes a unique strategy for biosensor enhancement by actively controlling the distribution of magnetic materials on the electrode surface through the application of a magnetic field, resulting in a visibly alternating stripe pattern. This approach effectively disperses magnetic particles, preventing their aggregation and reducing the boundary layer thickness, enhancing the electrochemical response of the biosensor. After fabrication condition optimization, CC is successfully detected using this biosensor. The fabricated sensor exhibits excellent performance with a wide linear detection range of 10-1000 µM, a low detection limit of 1.25 µM, and a sensitivity of 7.9 µA/mM. The fabricated sensor exhibits good selectivity and reliable detection in real water samples. In addition, the laccase-based sensor has the potential for the fast and accurate monitoring of CC in olive oil. SIGNIFICANCE: The magnetic field optimization in this study significantly improved the performance of the electrochemical biosensor for detecting CC in environmental samples. Overall, the sensor developed in this study has the potential for fast and accurate monitoring of CC in environmental samples, highlighting the potential importance of a magnetic field environment in improving the performance of catechol electrochemical biosensors.
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Técnicas Biosensibles , Catecoles , Técnicas Electroquímicas , Lacasa , Catecoles/análisis , Catecoles/química , Lacasa/química , Lacasa/metabolismo , Campos Magnéticos , Nanopartículas de Magnetita/química , Electrodos , Propiedades de Superficie , Límite de Detección , Enzimas Inmovilizadas/química , Enzimas Inmovilizadas/metabolismo , Contaminantes Químicos del Agua/análisisRESUMEN
The utilization of essential oils as natural antioxidants and preservatives is limited by high volatility, poor water solubility, and long-term instability. To address this, a novel ultrasonic-assisted method was used to prepare and stabilize a nanoemulsion of turmeric essential oil-in-water, incorporating bioactive components extracted from Spirulina platensis. Ultrasonic treatment enhanced the extraction efficacy and nanoemulsion stability. Algal biomass subjected to ultrasonic treatment (30 min at 80% amplitude) yielded a dry extract of 73.66 ± 3.05%, with the highest protein, phenolic, phycocyanin, and allophycocyanin content, as well as maximum emulsifying activity. The resulting nanoemulsion (5% oil, 0.3% extract, 10 min ultrasonic treatment) showed reduced particle size (173.31 ± 2.24 nm), zeta potential (-36.33 ± 1.10 mV), low polydispersity index, and enhanced antioxidant and antibacterial properties. Rheology analysis indicated shear-thinning behavior, while microscopy and spectroscopy confirmed structural changes induced by ultrasonic treatment and extract concentration. This initiative developed a novel ultrasonic-assisted algal-based nanoemulsion with antioxidant and antibacterial properties.
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Antibacterianos , Antioxidantes , Curcuma , Emulsiones , Aceites Volátiles , Spirulina , Spirulina/química , Antioxidantes/química , Antioxidantes/farmacología , Antioxidantes/aislamiento & purificación , Emulsiones/química , Aceites Volátiles/química , Aceites Volátiles/farmacología , Curcuma/química , Antibacterianos/farmacología , Antibacterianos/química , Tecnología Química Verde , Ultrasonido , Tamaño de la Partícula , Extractos Vegetales/química , Extractos Vegetales/farmacología , Extractos Vegetales/aislamiento & purificación , Agua/químicaRESUMEN
The augmented prevalence of Se (Se) pollution can be attributed to various human activities, such as mining, coal combustion, oil extraction and refining, and agricultural irrigation. Although Se is vital for animals, humans, and microorganisms, excessive concentrations of this element can give rise to potential hazards. Consequently, numerous approaches have been devised to mitigate Se pollution, encompassing physicochemical techniques and bioremediation. The recognition of Se volatilization as a potential strategy for mitigating Se pollution in contaminated environments is underscored in this review. This study delves into the volatilization mechanisms in various organisms, including plants, microalgae, and microorganisms. By assessing the efficacy of Se removal and identifying the rate-limiting steps associated with volatilization, this paper provides insightful recommendations for Se mitigation. Constructed wetlands are a cost-effective and environmentally friendly alternative in the treatment of Se volatilization. The fate, behavior, bioavailability, and toxicity of Se within complex environmental systems are comprehensively reviewed. This knowledge forms the basis for developing management plans that aimed at mitigating Se contamination in wetlands and protecting the associated ecosystems.
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Rotifer reproduction control in open microalgae cultivation systems poses a significant challenge for large-scale industries. Conventional methods, such as electric, meshing, and chemical techniques, are often expensive, ineffective, and may have adverse environmental-health impacts. This study investigated a promising control technique through light-induced phototaxis to concentrate rotifers in a specific spot, where they were electroshocked by local-limited exposure dose. The results showed that the rotifers had the most pronounced positive and negative phototropism with phototaxis rates of 66.7 % and -78.8 %, respectively, at blue-light irradiation of 30 µmolâm-2âs-1 and red-light irradiation of 22.5 µmolâm-2âs-1 for 20 min. The most effective electroshock configuration employed 1200 V/cm for 15 min with a 1-second cycle time and a 10 % duty cycle, resulting in a 75.0 % rotifer removal rate without impacting microalgae growth. The combination of the two light beams could effectively lead rotifers to designated areas where they were electrocuted successfully.
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Microalgas , Estanques , Fototaxis , Electrochoque , Luz Azul , BiomasaRESUMEN
The design of novel electrode deflector structures (EDSs) introduced a promising strategy for enhancing raceway ponds performance, increasing carbon fixation, and improving microalgal biomass accumulation. The computational fluid dynamics, based flow field principles, proved that the potency of arc-shaped electrode deflector structures (A-EDS) and spiral electrode deflector structures (S-EDS) were optimal. These configurations yielded superior culture effects, notably reducing dead zones by 9.1% and 11.7%, while elevating biomass increments of 14.7% and 11.5% compared to the control, respectively. In comparison to scenarios without electrostatic field application, the A-EDS group demonstrated pronounced post-stimulation growth, exhibiting an additional biomass increase of 11.2%, coupled with a remarkable 23.6% surge in CO2 fixation rate and mixing time reduction by 14.7%. A-EDS and S-EDS, combined with strategic electric field integration, provided a theoretical basis for promoting microalgal biomass production and enhancing carbon fixation in a raceway pond environment to similar production practices.
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Microalgas , Estanques , Biomasa , HidrodinámicaRESUMEN
This study focuses on microbial protein (MP) as a promising food-feed alternative source that may contribute to overcoming the increased food challenge. It analyzes the traditional and advanced MP technologies, their progress, sustainability, and environmental limitations. Traditional MP technologies are reliable for global food-feed supply chains but face higher production costs and negative environmental impacts. Advanced MP systems utilize sustainable sources like food waste, but limited availability and characteristics necessitate pretreatments. Power-to-protein technology looks promising due to its ability to capture CO2 and avoiding external organic carbon addition, although more research is still needed. Cultivating indigenous microorganisms in agricultural wastewater, such as biofloc technology, offer potential for nutrient recovery and reduced environmental impacts. Microalgal biomass is sustainable but faces challenges of low palatability, productivity, and high costs, while ongoing studies try to solve these challenges. This review concludes that the advanced MP technologies are environmentally friendly and promising, while further studies are necessary to enhance performance and commercial implementation.
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Microalgas , Eliminación de Residuos , Alimentos , Aguas Residuales , Ambiente , Biomasa , Tecnología , Microalgas/metabolismoRESUMEN
An efficient strategy for phycobiliprotein extraction from Spirulina platensis dry biomass has been developed by using NaCl as an enhancer. Different sodium ion and chloride ion salts were screened, and NaCl was selected as the most appropriate solvent for phycobiliprotein extraction. The extraction parameters with NaCl were optimized using response surface methodology. Under optimal operating conditions, a phycobiliprotein extraction rate of 74.8 % and a phycocyanin extraction yield of 102.4 mg/g with a purity of 74.0 % were achieved. Adding NaCl resulted in smaller fragments and destroyed the cell integrity of S. platensis, facilitating phycobiliprotein exudation. The secondary structure and antioxidant activity of phycobiliproteins were not affected by NaCl extraction. The stability of the phycobiliproteins was improved by adding NaCl. This study provides a potential method for phycobiliprotein extraction with high efficiency and good quality using an inexpensive extraction enhancer.
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Ficobiliproteínas , Spirulina , Cloruro de Sodio , Biomasa , Spirulina/química , Ficocianina/químicaRESUMEN
In this study, computational fluid dynamics were employed to examined clockwise and anticlockwise vortexes in the rising and down coming sections of novel nested-bottle photobioreactor. The radial velocity was increased by four times which significantly reduced dead zones compared to traditional PBR. The (NB-PBR) comprised of integrated bottles connected by curved tubes (d = 4 cm) that generated dominant vortices as the microalgae solution flows through each section (h = 10 cm). The (NB-PBR) was independent of the inner and outer sections which increased the mixing time and mass-transfer coefficient by 13.33 % and 42.9 %, respectively. Furthermore, the results indicated that the (NB-PBR) showed higher photosynthesis efficiency preventing self-shading and photo-inhibition, resulting in an increase in biomass yield and carbon dioxide fixation by 35 % and 35.9 %, respectively.