Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4.

The inorganic selenium is absorbed and utilized inefficiently, and the range between toxicity and demand is narrow, so the application is strictly limited. Selenium nanoparticles have higher bioactivity and biosafety properties, including increased antioxidant and anticancer properties. Thus, producing and applying eco-friendly, non-toxic selenium nanoparticles in feed additives is crucial. Bacillus paralicheniformis Y4 was investigated for its potential ability to produce selenium nanoparticles and the activity of carboxymethyl cellulases. The selenium nanoparticles were characterized using zeta potential analyses, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). Additionally, evaluations of the anti-α-glucosidase activity and the antioxidant activity of the selenium nanoparticles and the ethyl acetate extracts of Y4 were conducted. B. paralicheniformis Y4 exhibited high selenite tolerance of 400 mM and the selenium nanoparticles had an average particle size of 80 nm with a zeta potential value of -35.8 mV at a pH of 7.0, suggesting that the particles are relatively stable against aggregation. After 72 h of incubation with 5 mM selenite, B. paralicheniformis Y4 was able to reduce it by 76.4%, yielding red spherical bio-derived selenium nanoparticles and increasing the carboxymethyl cellulase activity by 1.49 times to 8.96 U/mL. For the first time, this study reports that the carboxymethyl cellulase activity of Bacillus paralicheniforis was greatly enhanced by selenite. The results also indicated that B. paralicheniformis Y4 could be capable of ecologically removing selenite from contaminated sites and has great potential for producing selenium nanoparticles as feed additives to enhance the added value of agricultural products.

Development of CRISPR-Cas9 genome editing system in Talaromyces marneffei.

Talaromyces marneffei is an important pathogenic thermally dimorphic fungus causing systemic talaromycosis mainly prevalent in Southeast Asia. The dimorphic transition between mycelium and yeast is considered crucial for the pathogenicity of T. marneffei. However, the lack of genetic toolbox has been a major impediment for understanding its pathogenicity. Here a CRISPR-Cas9 system was developed to facilitate genetic manipulations in this organism. In this study, the CRISPR-Cas9 gene editing system uses a native U6 snRNA promoter from T. marneffei to drive the expression of sgRNA. Employing this system and PEG-mediated protoplast transformation, the sakA gene was mutated. Sanger sequencing confirmed nearly 40% site-directed mutation rate. The phenotype analysis confirmed the sakA gene function in T. marneffei dimorphic transition. Our study provided a powerful genome-manipulating tool, which could accelerate studies on T. marneffei for further revealing the mechanisms of its pathogenicity.

Outcomes of secondary autologus dermo-fat orbital implants in anophthalmic sockets.

OBJECTIVES: To evaluate the outcomes of secondary autologous dermis-fat graft as an orbital implant in anophthalmic sockets. METHODS: In this prospective study, which was conducted at Jinnah Post Graduate Medical Centre, Karachi, between January 2015 and January 2020, we evaluated 12 patients between the ages of four and 60 years. Most of the adults were victims of trauma, whereas children were known cases of retinoblastoma or trauma and all underwent enucleation. All of them were primarily treated elsewhere and not offered primary orbital implants. We performed autologous dermis-fat graft as an orbital implant in these patients harvesting graft from gluteal region and followed them up to look for complications. RESULTS: Out of 12 patients two went into failure, while rest of the patients showed successful outcome. All patients underwent successful surgery. Initially, a silicon conformer was placed, which was later on replaced with artificial prosthetic eye. CONCLUSION: Regardless of the small sample size, this procedure proved to be a safe and effective method for augmenting orbital volume in anophthalmic sockets in children and adults.

RNA interference-mediated knockdown of a cytochrome P450 gene enhanced the toxicity of α-cypermethrin in xanthotoxin-fed larvae of Spodoptera exigua (Hübner).

The beet armyworm (Spodoptera exigua) is a highly polyphagous agricultural pest that is distributed worldwide. However, the adaptive mechanisms of S. exigua for various insecticides and defensive substances in host plants are unknown. Insect P450 monooxygenases play an important role in the detoxification of plant toxins and insecticides, leading to insecticides resistance. We investigated the induced effects of xanthotoxin exposure on detoxification enzyme activity and larval tolerance to α-cypermethrin in S. exigua. Our results showed that the lethal concentration (LC50) of α-cypermethrin for xanthotoxin-exposed larvae was 2.1-fold higher than in the control. Moreover, cytochrome P450 enzyme activity was significantly elevated by upregulation of P450 genes in treated larvae. RT-qPCR results showed that CYP9A10 expression level was significantly increased in all treatments, while maximal expression level was observed in xanthotoxin+α-cypermethrin-fed larvae. RNAi-mediated silencing of CYP9A10 further increased mortality by 18%, 26% and 35% at 48 h and by 27%, 43% and 55% at 72 h when larvae were exposed to diets containing chemicals as compared to the control. The results show that CYP9A10 might play an important role in xanthotoxin and α-cypermethrin detoxification in S. exigua. RNAi-mediated silencing could provide an effective synergistic agent for pest control or insecticide resistance management.

Interaction-Dependent Interfacial Charge-Transfer Behavior in Solar Water-Splitting Systems.

Dual-band-gap systems are promising for solar water splitting due to their excellent light-harvesting capability and high charge-separation efficiency. However, a fundamental understanding of interfacial charge-transfer behavior in the dual-band-gap configuration is still incomplete. Taking CdS/reduced graphene oxide (CdS/RGO) nanoheterojunctions as a model solar water splitting system, we attempt here to highlight the interaction-dependent interfacial charge-transfer behavior based on both experimental observations and theoretical calculations. Experimental evidence points to charge transfer at the CdS-RGO interface playing a dominant role in the photocatalytic hydrogen production activity. By tuning the degree of reduction of RGO, the interfacial interaction, and, thereby, the charge transfer can be controlled at the CdS-RGO interface. This observation is supported by theoretical analysis, where we find that the interfacial charge transfer is a balance between the effective single-electron- and hole-transfer probability and the surface free electron and hole concentration, both of which are related to the surface potential and tailored by interfacial interaction. This mechanism is applicable to all systems for solar water splitting, providing a useful guidance for the design and study of heterointerfaces for high-efficiency energy conversion.

Enhanced effects of dietary tannic acid with chlorantraniliprole on life table parameters and nutritional physiology of Spodoptera exigua (Hübner).

The beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) is a highly polyphagous pest which causes considerable economic losses to cotton and many vegetable crops. Tannins are among the most important secondary metabolites in cotton plants. We show that tannic acid enhances the toxic effect of chlorantraniliprole on S. exigua when presented in combination. Bioassays using third-instar S. exigua larvae on an artificial diet showed that consumption of tannic acid with chlorantraniliprole at the concentration of (2 mg/g and LC50 0.018 mg/L) had higher toxicity when compared to either chlorantraniliprole or tannic acid alone (LC50 0.027 mg/L). The diet containing tannic acid with chlorantraniliprole significantly prolonged larval and pupal developmental time and extended mean generation time and total pre-oviposition period compared to either chemical alone. Moreover, fecundity, survival rate, reproductive value, intrinsic rate of increase, finite rate of increase and net reproduction rate declined significantly when exposed to the combined treatment. No difference was observed between tannic acid and the control. Meanwhile, tannic acid with chlorantraniliprole had markedly antifeedant effects; causing significant decline in the relative growth rate (RGR), the relative consumption rate (RCR), the efficiency of conversion of ingested food (ECI), the efficiency of conversion of digested food and an increase in the approximate digestibility (AD) compared to either chemical alone. Tannic acid with chlorantraniliprole also decreased the insect's carbohydrate, lipid and protein contents significantly. The results showed that the interaction between tannic acid and chlorantraniliprole on the growth inhibition of larvae was additive and tannic acid increased the toxicity of chlorantraniliprole to insects. The results of this study provide information useful in integrated pest management programs for S. exigua and show that tannic acid combined with chlorantraniliprole may be a route to reducing the use of synthetic pesticides.

Facile Integration between Si and Catalyst for High-Performance Photoanodes by a Multifunctional Bridging Layer.

Designing high-quality interfaces is crucial for high-performance photoelectrochemical (PEC) water-splitting devices. Here, we demonstrate a facile integration between polycrystalline n+p-Si and NiFe-layered double hydroxide (LDH) nanosheet array by a partially activated Ni (Ni/NiOx) bridging layer for the excellent PEC water oxidation. In this model system, the thermally deposited Ni interlayer protects Si against corrosion and makes good contact with Si, and NiOx has a high capacity of hole accumulation and strong bonding with the electrodeposited NiFe-LDH due to the similarity in material composition and structure, facilitating transfer of accumulated holes to the catalyst. In addition, the back illumination configuration makes NiFe-LDH sufficiently thick for more catalytically active sites without compromising Si light absorption. This earth-abundant multicomponent photoanode affords the PEC performance with an onset potential of ∼0.78 V versus reversible hydrogen electrode (RHE), a photocurrent density of ∼37 mA cm-2 at 1.23 V versus RHE, and retains good stability in 1.0 M KOH, the highest water oxidation activity so far reported for the crystalline Si-based photoanodes. This bridging layer strategy is efficient and simple to smooth charge transfer and make robust contact at the semiconductor/electrocatalyst interface in the solar water-splitting systems.

Vertically Aligned Porous Organic Semiconductor Nanorod Array Photoanodes for Efficient Charge Utilization.

Because of inefficient charge utilization caused by localized π-electron conjugation and large exciton binding energy, the photoelectrochemical water-splitting efficiency of organic polymers is seriously limited. Taking the graphitic carbon nitride (g-CN) polymer as an example, we report a novel photoanode based on a vertically aligned g-CN porous nanorod (PNR) array prepared in situ, using a thermal polycondensation approach, with anodic aluminum oxide as the template. The g-CN PNR array exhibits an excellent photocurrent density of 120.5 µA cm-2 at 1.23 VRHE under one sun illumination, the highest reported incident photon-to-current efficiency of ∼15% at 360 nm, and an outstanding oxygen evolution reaction stability in 0.1 M Na2SO4 aqueous solution, which constitutes a benchmark performance among the reported g-CN-based polymer photoanodes without any sacrificial reagents. When compared with its planar counterpart, the enhanced performance of the PNR array results principally from its unique structure that enables a high degree of aromatic ring π-electron conjugation for higher mobility of charge carriers, provides a direct pathway for the electron transport to the substrate, produces a large portion of hole-accepting defect sites and space charge region to promote exciton dissociation, and also withstands more strain at the interface to ensure intimate contact with the substrate. This work opens a new avenue to develop nanostructured organic semiconductors for large-scale application of solar energy conversion devices.

Knock-Down of Gossypol-Inducing Cytochrome P450 Genes Reduced Deltamethrin Sensitivity in Spodoptera exigua (Hübner).

Plants employ an intricate and dynamic defense system that includes physiological, biochemical, and molecular mechanisms to counteract the effects of herbivorous attacks. In addition to their tolerance to phytotoxins, beet armyworm has quickly developed resistance to deltamethrin; a widely used pyrethroid insecticide in cotton fields. The lethal concentration (LC50) required to kill 50% of the population of deltamethrin to gossypol-fed Spodoptera exigua larvae was 2.34-fold higher than the control group, suggesting a reduced sensitivity as a consequence of the gossypol diet. Piperonyl butoxide (PBO) treatment was found to synergize with deltamethrin in gossypol-fed S. exigua larvae. To counteract these defensive plant secondary metabolites, beet armyworm elevates their production of detoxification enzymes, including cytochrome P450 monooxygenases (P450s). Gossypol-fed beet armyworm larvae showed higher 7-ethoxycoumarin-O-deethylase (ECOD) activities and exhibited enhanced tolerance to deltamethrin after 48 and 72 h when compared to the control. Moreover, gossypol pretreated S. exigua larvae showed faster weight gain than the control group after transferring to a deltamethrin-supplemented diet. Meanwhile, gossypol-induced P450s exhibited high divergence in the expression level of two P450 genes: CYP6AB14 and CYP9A98 in the midgut and fat bodies contributed to beet armyworm tolerance to deltamethrin. Knocking down of CYP6AB14 and CYP9A98, via double-stranded RNAs (dsRNA) in a controlled diet, rendered the larvae more sensitive to the insecticide. These data demonstrate that generalist insects can exploit secondary metabolites from host plants to enhance their defense systems against other toxic chemicals. Impairing this defense pathway by RNA interference (RNAi) holds a potential to eliminate the pest's tolerance to insecticides and, therefore, reduce the required dosages of agrochemicals in pest control.

Microscopic cuticle structure comparison of pupal melanic and wild strain of Spodoptera exigua and their gene expression profiles in three time points.

The beet armyworm, Spodoptera exigua (Hubner), is one of the major crop pests and is a target for current pest control approaches using insecticides. S. exigua melanic mutants (SEM) spontaneously occurred in the S. exigua wild type (SEW) strain and have been maintained under laboratory conditions on an artificial diet. Scanning electron microscopy showed that the inner cuticle of the SEM had a denser and less orderly structure. We investigated the cuticle protein genes using RNA-seq at three different developmental stages of both SEM and SEW. Comparison of cDNA libraries showed that 7257 CPs were significantly up-regulated and 664 genes were significantly downregulated in SEM at the developmental stage of 46-h in the fifth instar. In addition, 460 genes were significantly up-regulated and 439 genes were significantly down-regulated in the SEM at the development stage of 4-h before pupation. Moreover, 162 genes were significantly up-regulated and 293 genes were significantly downregulated in the SEM, just after pupation. Two genes CPR63 and CPR97 were identified from RNA sequences to verify the differentially expressed gene (DEG) results through quantitative real-time PCR (qRT-PCR). The results show that expression of both CPR63 and CPR97 structural cuticular proteins were significantly different between SEM and SEW. This functional analysis may help in understanding the role that these genes play in the cuticle pattern of the SEM.

Outcomes of Propeller Flap in Eyelid Reconstruction.

The purpose of this case report is to demonstrate the outcomes of reconstruction in small-to-moderate-sized benign tumors of eyelids by using the propeller flap technique. Propeller flaps have been used by plastic surgeons for facial reconstruction and other body parts. However, very few oculoplastic surgeons have utilized this technique in eyelid reconstructive surgeries. We have substantiated this technique and its outcomes in this case report. It is a case series consisted of two patients reporting in the orbit-oculoplastics clinic with suspicious basal cell carcinoma of eyelids, covering less than half of the eyelid. Both the patients underwent tumor excision and reconstruction, with a cutaneous propeller flap supplied by a pedicle. Both the patients recovered well and without any complication. The specimens removed from both the patients were sent for histopathology and the biopsy results revealed both lesions were margin-free basal cell carcinoma. The reconstruction of eyelid defects is challenging due to the small area and cosmetic appearance. Propeller flaps are a reasonable choice of treatment in small to moderately large-sized defects with minimal complications, yet better cosmetic appearance.

Harmony but Not Uniformity: Role of Strigolactone in Plants.

Strigolactones (SLs) represent an important new plant hormone class marked by their multifunctional roles in plants and rhizosphere interactions, which stimulate hyphal branching in arbuscular mycorrhizal fungi (AMF) and seed germination of root parasitic plants. SLs have been broadly implicated in regulating root growth, shoot architecture, leaf senescence, nodulation, and legume-symbionts interaction, as well as a response to various external stimuli, such as abiotic and biotic stresses. These functional properties of SLs enable the genetic engineering of crop plants to improve crop yield and productivity. In this review, the conservation and divergence of SL pathways and its biological processes in multiple plant species have been extensively discussed with a particular emphasis on its interactions with other different phytohormones. These interactions may shed further light on the regulatory networks underlying plant growth, development, and stress responses, ultimately providing certain strategies for promoting crop yield and productivity with the challenges of global climate and environmental changes.

Selective Growth of Stacking Fault Free ⟨100⟩ Nanowires on a Polycrystalline Substrate for Energy Conversion Application.

Cubic semiconductor nanowires grown along ⟨100⟩ directions have been reported to be promising for optoelectronics and energy conversion applications, owing to their pure zinc-blende structure without any stacking fault. But, until date, only limited success has been achieved in growing ⟨100⟩ oriented nanowires. Here we report the selective growth of stacking fault free ⟨100⟩ nanowires on a commercial transparent conductive polycrystalline fluorine-doped SnO2 (FTO) glass substrate via a simple and cost-effective chemical vapor deposition (CVD) method. By means of crystallographic analysis and density functional theory calculation, we prove that the orientation relationship between the Au catalyst and the FTO substrate play a vital role in inducing the selective growth of ⟨100⟩ nanowires, which opens a new pathway for controlling the growth directions of nanowires via the elaborate selection of the catalyst and substrate couples during the vapor-solid-liquid (VLS) growth process. The ZnSe nanowires grown on the FTO substrate are further applied as a photoanode in photoelectrochemical (PEC) water splitting. It exhibits a higher photocurrent than the ZnSe nanowires do without preferential orientations on a Sn-doped In2O3 (ITO) glass substrate, which we believe to be correlated with the smooth transport of charge carriers in ZnSe ⟨100⟩ nanowires with pure zinc-blende structures, in distinct contrast with the severe electron scattering happened at the stacking faults in ZnSe nanowires on the ITO substrate, as well as the efficient charge transfer across the intensively interacting nanowire-substrate interfaces.

Expression and functional analysis of P450 gene induced tolerance/resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hübner).

Beet armyworm, Spodoptera exigua (Hübner) is an agronomical important and most devastating polyphagous pest that damages a variety of crops around the globe including China. Quercetin is one of the abundant dietary flavonoids and the important defense allelochemicals in plants. Therefore, the changes in insect detoxification enzymes activities in response to plants allelochemicals may result increased the sensitivity to insecticides. In this study, we examined the induced effect of quercetin on larval tolerance to lambda-cyhalothrin in S. exigua. Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly synergized the lambda-cyhalothrin toxicity in quercetin-fed S. exigua larvae. Moreover, larval weight significantly reduced in quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin treatment. Furthermore, our results showed that the P450 detoxification enzyme effectively increased in all treatments as compared to the control. Quantitative Real-time PCR analysis revealed that expression level of CYP6AE10 significantly upregulated in larvae treated with quercetin, lambda-cyhalothrin and quercetin + lambda-cyhalothrin in the midgut and fat body respectively. In addition, RNAi mediated knockdown of CYP6AE10 in S. exigua larvae significantly decreased the transcription level of target cytochrome P450 gene followed by the exposure with quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin. Similarly, the knockdown of CYP6AE10 by the injection of dsRNA led to increased mortality after the treatment with respective chemicals. Overall, these data showed that P450s might possibly play an important role in the metabolic adaptation of S. exigua larvae to its host plant defense allelochemicals as well as insecticides. In conclusion, S. exigua can take benefit from its host plant's secondary metabolites to elaborate its defense against synthetic insecticides.

Characterization of mycotoxins from entomopathogenic fungi (Cordyceps fumosorosea) and their toxic effects to the development of asian citrus psyllid reared on healthy and diseased citrus plants.

Entomopathogenic fungi (EPF) produce multiple mycotoxins, which play an essential role in improving fungal pathogenesis and virulence. To characterize various mycotoxins from the crude methanol extract of Cordyceps fumosorosea, a major EPF against various insect pests, we performed ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometer (UPLC-QTOF MS) technique, and all compounds were identified through molecular mass and formulae. Bassianolide was assessed against the nymphs and adults of Diaphorina citri reared on healthy and Huánglóngbìng (HLB)-diseased Citrus spp. Plants under laboratory conditions. Overall, 17 compounds were identified from the fungal extract and categorized into three groups, i.e. (1) alkaloids (Isariotins A-C), (2) peptides (Bassianolide, Beauverolides, Beauvericin A, Isaridins and Destruxin E) and (3) polyketide (Tenuipyrone). The detected beauverolides (B, C, F, I, Ja) from C. fumosorosea were novel mycotoxins, and their detection intensity was the highest in the fungal extract. Furthermore, bassianolide caused more than 70% and 80% mortality of D. citri nymphs and adults after two days of application, respectively. After three days of chemical application, all nymphal and adult populations of D. citri were killed by bassianolide. However, the mortality rates of both populations, nymphs and adults, were higher on HLB-diseased plants as compared to healthy plants.

Sub-lethal effects of lufenuron exposure on spotted bollworm Earias vittella (Fab): key biological traits and detoxification enzymes activity.

Spotted bollworm, Earias vittella, is one of the most serious and devastating insect pests of vegetables and cotton. Currently, insecticides are necessary for its control in nearly all crop systems. In this paper, we evaluate the sub-lethal effects of lufenuron on biological traits and activity of detoxification enzymes: cytochrome P450 monooxygenases, esterase, and glutathione S-transeferase (GST) in second instar larvae of E. vittella. Results showed that sub-lethal concentrations (LC15 and LC40 of lufenuron), prolonged larval period (at LC40 = 13.86 ± 1.22 day, LC15 = 13.14 ± 1.15 day, control = 12.28 ± 0.7), pupal duration (LC40 = 11.1 ± day, LC15 = 11.8 ± 0.28 day, control = 9.40 ± 0.52), and extended mean generation time (LC40 = 27.3 ± 0.43 LC15 = 29.0 ± 1.19 day, control = 26.0 ± 0.65). Sub-lethal exposure significantly prolonged the pre-adult stage, decreased pupal weight, and reduced adult longevity in the parent (F0) and F1 generation. Moreover, the fecundity and egg viability were significantly lowered in parental and F1 generations at both sub-lethal concentrations compared to the control. While no significant effects were noted on reproductive parameters such as the intrinsic rate of increase (r), finite rate of increase (λ), and net reproduction rate (R0) of F1 generation when compared to the control. Only mean generation time (T) in F1 at LC15 was significantly longer compared to the LC40 and control (LC40 = 3.79 ± 0.37, LC15 = 32.28 ± 1.55 day, control = 29.79 ± 0.55). Comparatively, the activities of cytochrome P450 monooxygenases and esterase were higher than GST in treated populations. The increase in resistance development against insecticides may possibly because of elevated activity of detoxification enzymes. These results provide useful information for monitoring resistance in integrated pest management (IPM) programs for E. vittella.

Gossypol-induced fitness gain and increased resistance to deltamethrin in beet armyworm, Spodoptera exigua (Hübner).

BACKGROUND: In plant-insect interactions, phytotoxins such as gossypol, exert a defensive role on behalf of the plant by interfering with the essential metabolic, biochemical and physiological pathways of herbivorous insects. The beet armyworm, Spodoptera exigua (Hübner), is a key pest for many important crops including a range of vegetables, ornamentals, and cotton. In this study, we investigated how resistance to deltamethrin relates to enzyme activity in gossypol-pretreated larvae of S. exigua. RESULTS: Following selection with deltamethrin insecticides on gossypol-pretreated larvae for 10 generations, the Gos-SEL population developed a 113.29-fold resistance. Under the same conditions, the Delta-SEL selected population showed a 69.76-fold increase in resistance along with corresponding levels of xenobiotic defense enzyme activity. Similarly, the fecundity of the Delta-SEL population together with male and female longevity were found to be significantly lower when compared with the Gos-SEL population and the laboratory susceptible-strain group (SS-Strain). In addition, the activities of cytochrome P450s in S. exigua were significantly enhanced when the insects were fed on a deltamethrin and gossypol-pretreated diet compared with being fed on deltamethrin alone. CONCLUSION: The reproductive capacity of S. exigua is significantly reduced in Delta-SEL and Gos-SEL populations compared with the control group (SS-Strain). Elevation of the major detoxification enzyme cytochrome P450 monooxygenase and esterase might have an important role in inducing tolerance to deltamethrin in gossypol-fed S. exigua populations. This study enhances our understanding of detoxification enzyme pathways for S. exigua gene expression and their role in responses to insecticides and plant secondary metabolites. © 2018 Society of Chemical Industry.

Antibacterial Carbon-Based Nanomaterials.

The emergence and global spread of bacterial resistance to currently available antibiotics underscore the urgent need for new alternative antibacterial agents. Recent studies on the application of nanomaterials as antibacterial agents have demonstrated their great potential for management of infectious diseases. Among these antibacterial nanomaterials, carbon-based nanomaterials (CNMs) have attracted much attention due to their unique physicochemical properties and relatively higher biosafety. Here, a comprehensive review of the recent research progress on antibacterial CNMs is provided, starting with a brief description of the different kinds of CNMs with respect to their physicochemical characteristics. Then, a detailed introduction to the various mechanisms underlying antibacterial activity in these materials is given, including physical/mechanical damage, oxidative stress, photothermal/photocatalytic effect, lipid extraction, inhibition of bacterial metabolism, isolation by wrapping, and the synergistic effect when CNMs are used in combination with other antibacterial materials, followed by a summary of the influence of the physicochemical properties of CNMs on their antibacterial activity. Finally, the current challenges and an outlook for the development of more effective and safer antibacterial CNMs are discussed.

Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026.

Huperzine A (HupA) isolated from Huperzia serrata is an important compound used to treat Alzheimer's disease (AD). Recently, HupA was reported in various endophytic fungi, with Colletotrichum gloeosporioides ES026 previously isolated from H. serrata shown to produce HupA. In this study, we performed next-generation sequencing and de novo RNA sequencing of C. gloeosporioides ES026 to elucidate the molecular functions, biological processes, and biochemical pathways of these unique sequences. Gene ontology and Kyoto Encyclopedia of Genes and Genomes assignments allowed annotation of lysine decarboxylase (LDC) and copper amine oxidase (CAO) for their conversion of L-lysine to 5-aminopentanal during HupA biosynthesis. Additionally, we constructed a stable, high-yielding HupA-expression system resulting from the overexpression of CgLDC and CgCAO from the HupA-producing endophytic fungus C. gloeosporioides ES026 in Escherichia coli. Quantitative reverse transcription polymerase chain reaction analysis confirmed CgLDC and CgCAO expression, and quantitative determination of HupA levels was assessed by liquid chromatography high-resolution mass spectrometry, which revealed that elevated expression of CgLDC and CgCAO produced higher yields of HupA than those derived from C. gloeosporioides ES026. These results revealed CgLDC and CgCAO involvement in HupA biosynthesis and their key role in regulating HupA content in C. gloeosporioides ES026.

Isolation and functional identification of three cuticle protein genes during metamorphosis of the beet armyworm, Spodoptera exigua.

The beet armyworm, Spodoptera exigua (Hubner), is one of the major crop pests and is a target for current pest control approaches using insecticides. In this study three cuticular protein genes CPG316, CPG860 and CPG4855 have been cloned from 0 h pupal integument of S. exigua through race PCR Strategy. The deduced amino acid sequences were found to contain the RR-2 consensus region of other insect cuticular proteins and construct phylogenetic trees for each protein. Using quantitative RT-PCR, the developmental expression of the three genes through several larval and the early pupal stages was studied. All three genes contribute to the endocuticle although CPG316 may have a different role from the other two genes. All three newly isolated genes were analyzed and their functions were determined by using direct injection of the dsRNA into early 5th instar larvae. All genes are expressed in the larvae and early pupae but in different patterns. Furthermore, phenotypic results show that these genes have differing effects on the development of cuticle, its flexibility and a big role in metamorphosis in both larval and pupal stages.