Phytosynthesis of zinc oxide nanoparticles for enhanced antioxidant, antibacterial, and photocatalytic properties: A greener approach to environmental sustainability.

Multifunctional nanoparticles (NPs) production from phytochemicals is a sustainable process and an eco-friendly method, and this technique has a variety of uses. To accomplish this, we developed zinc oxide nanoparticles (ZnONPs) using the medicinal plant Tinospora cordifolia (TC). Instruments such as UV-Vis, XRD, FTIR, FE-SEM with EDX, and high-resolution TEM were applied to characterize the biosynthesized TC-ZnONPs. According to the UV-vis spectra, the synthesized TC-ZnONPs absorb at a wavelength centered at 374 nm, which corresponds to a 3.2 eV band gap. HRTEM was used to observe the morphology of the particle surface and the actual size of the nanostructures. TC-ZnONPs mostly exhibit the shapes of rectangles and triangles with a median size of 21 nm. The XRD data of the synthesized ZnONPs exhibited a number of peaks in the 2θ range, implying their crystalline nature. TC-ZnONPs proved remarkable free radical scavenging capacity on DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), and NO (Nitric Oxide). TC-ZnONPs exhibited dynamic anti-bacterial activity through the formation of inhibition zones against Pseudomonas aeruginosa (18 ± 1.5 mm), Escherichia coli (18 ± 1.0 mm), Bacillus cereus (19 ± 0.5 mm), and Staphylococcus aureus (13 ± 1.1 mm). Additionally, when exposed to sunlight, TC-ZnONPs show excellent photocatalytic ability towards the degradation of methylene blue (MB) dye. These findings suggest that TC-ZnONPs are potential antioxidant, antibacterial, and photocatalytic agents.

Acaricidal, pediculicidal and larvicidal activity of synthesized ZnO nanoparticles using Momordica charantia leaf extract against blood feeding parasites.

The aim of the present study was to evaluate the acaricidal, pediculicidal and larvicidal effect of synthesized zinc oxide nanoparticles (ZnO NPs) using Momordica charantia leaf extract against the larvae of Rhipicephalus (Boophilus) microplus, adult of Pediculus humanus capitis, and the larvae of Anopheles stephensi, Culex quinquefasciatus. The ZnO NPs were characterized by using UV, XRD, FTIR and SEM-EDX. The SEM image confirms that the synthesized nanoparticles were spherical in shape with a size of 21.32 nm. The results of GC-MS analysis indicates the presence of the major compound of Nonacosane (C29H60) in the M. charantia leaf extract. Cattle tick, head lice and mosquito larvae were exposed to a varying concentrations of the synthesized ZnO NPs and M. charantia leaf extract for 24 h. Compared to the leaf aqueous extract, biosynthesized ZnO NPs showed higher toxicity against R. microplus, P. humanus capitis, An. stephensi, and Cx. Quinquefasciatus with the LC50 values of 6.87, 14.38, 5.42, and 4.87 mg/L, respectively. The findings revealed that synthesized ZnO NPs possess excellent anti-parasitic activity. These results suggest that the green synthesized ZnO NPs has the potential to be used as an ideal ecofriendly approach for the control of R. microplus, P. humanus capitis and the mosquito larvae of An. Stephensi and Cx. quinquefasciatus.

GC-MS analysis of bioactive components and biosynthesis of silver nanoparticles using Hybanthus enneaspermus at room temperature evaluation of their stability and its larvicidal activity.

Green synthesis of silver nanoparticles (AgNPs) using Hybanthus enneaspermus extract at room temperature that act as a reducing agent as well as capping agent has been investigated. The synthesized AgNPs were characterized by UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR), zeta potential, and dynamic light scattering (DLS) transmission electron microscopy (TEM) and energy-dispersive X-ray (EDX). The silver surface plasmon resonance was observed at 420 nm in the UV-visible spectrum. XRD peaks were observed at 2θ values in 38.20°, 44.40°, 64.60°, and 77.50° which are indexed as (111), (200), (220), and (311) bands of face-centered cubic (fcc) structures of silver. FTIR revealed the AgNPs were capped with plant compounds of alcohol, phenols, carbonyl, amines, and amide functional groups. TEM image shows that the particles were of spherical, hexagonal, and triangular in shape, and the size range was 16-26 nm. Further, DLS exhibits the average size of 25.2 nm and the zeta values were measured (-27.1 mV) which proves the stability of the AgNPs. The conversion of Ag(+) ions into Ag(0) was calculated using inductively coupled plasma atomic emission spectroscopy (ICP-MS) and was found to be 96 %. The biosynthesized AgNPs showed the larvicidal activity with the LC50 values of 17.24 and 13.12 mg/L against the fourth-instar larvae of Anopheles subpictus and Culex quinquefasciatus, respectively. The GC-MS analysis of the plant extract showed that 39 bioactive phytochemical compounds have been found to possess a wide range of activities, which may help in the protection against incurable diseases.

Novel microbial route to synthesize ZnO nanoparticles using Aeromonas hydrophila and their activity against pathogenic bacteria and fungi.

In the present work, we describe a low-cost, unreported and simple procedure for biosynthesis of zinc oxide nanoparticles (ZnO NPs) using reproducible bacteria, Aeromonas hydrophila as eco-friendly reducing and capping agent. UV-vis spectroscopy, XRD, FTIR, AFM, NC-AFM and FESEM with EDX analyses were performed to ascertain the formation and characterization of ZnO NPs. The synthesized ZnO NPs were characterized by a peak at 374 nm in the UV-vis spectrum. XRD confirmed the crystalline nature of the nanoparticles and AFM showed the morphology of the nanoparticle to be spherical, oval with an average size of 57.72 nm. Synthesized ZnO NPs showed the XRD peaks at 31.75°, 34.37°, 47.60°, 56.52°, 66.02° and 75.16° were identified as (100), (002), (101), (102), (110), (112) and (202) reflections, respectively. Rietveld analysis to the X-ray data indicated that ZnO NPs have hexagonal unit cell at crystalline level. The size and topological structure of the ZnO NPs was measured by NC-AFM. The morphological characterization of synthesized nanoparticles was analyzed by FESEM and chemical composition by EDX. The antibacterial and antifungal activity was ended with corresponding well diffusion and minimum inhibitory concentration. The maximum zone of inhibition was observed in the ZnO NPs (25 µg/mL) against Pseudomonas aeruginosa (22±1.8 mm) and Aspergillus flavus (19±1.0 mm). Bacteria-mediated ZnO NPs were synthesized and proved to be a good novel antimicrobial material for the first time in this study.

Effects of parathyroid hormone on blood flow in different regional circulations.

Parathyroid hormone and, in particular, its 1-34 aminoterminal fragment, PTH-(1-34), are potent vasodilators of the coronary circulation. In addition the hormone exerts a powerful hypotensive effect in a variety of animals, suggesting that the polypeptide hormone is vasoactive in peripheral regional circulations as well. The purpose of this study was to determine the regional circulations that were responsive and the relative sensitivity of each to the vasoactive properties of the hormone fragment. An anesthetized instrumented open-chest and/or open-abdomen dog model was used. Blood flow was continuously monitored in left circumflex, left pulmonary, right renal, celiac, and superior mesenteric arteries. Doses of synthetic human PTH-(1-34) ranging from 0.00024 to 0.24 nmol/kg were administered by bolus intra-arterial injections. Although PTH-(1-34) produced a dose-related vasodilation in each of the above regional vascular beds, marked differences in sensitivity were observed. The peptide elicited large increases in coronary (178%) and celiac (162%) blood flow. Maximal renal and pulmonary responses were relatively small (increases of 30 and 23%, respectively). Compared with control values, blood flow in the superior mesenteric circulation increased only at the highest dose tested. Thus sensitivity to the vascular effects of the hormone fragment differs markedly between the regional vascular beds of the dog.