Bio-electrospraying 3-D Organotypic Human Skin Cultures.

Organotypic 3D tissue models have greatly contributed to understand a wide range of molecular and cellular characteristics within a functional or diseased tissue. Human skin reconstructs which act as models are most useful for a wide range of investigations, ranging from tissue engineering and regenerative medicine, drug development, screening, and discovery to name a few. There are many approaches for reconstructing 3D skin tissue models, however, to date there have been very few that are able to generate organotypic 3D constructs with a single technology having minimal processing steps to finally scalability. The many manifestations of 3D bioprinting have contributed to this endeavor, having said that, the technology's limitations have tempered those reconstructed models, as they are known to contain low cell numbers/concentrations to those having damaged/dead molecules/cells within the reconstructed tissue, which are not desirable, for exploring as tissues models. Contrary to 3D bioprinting approaches, bio-electrosprays have been demonstrated to possess the ability to handle large concentrations of cells and molecules to whole fertilized embryos without damaging them from a molecular level upwards. Consequently, this article demonstrates, for the first time, bio-electrospray's capacity to reconstruct skin-like structures in vitro and its potential in reconstructing full-thickness 3D organotypic human skin tissues.

Unidirectional ring laser operation and tunable single-frequency emission using differential parametric gain.

In this Letter, a novel approach for unidirectional operation of a 1064 nm solid-state ring laser is demonstrated based on difference frequency mixing. Unidirectional operation is achieved exploiting the directional parametric gain from a single-pass diode laser, facilitated through a periodically poled LiNbO3 crystal. In addition to achieving unidirectional operation, the nonlinear process further enables the generation of single-frequency mid-infrared light. Using a single-pass tapered diode laser, tunable in the range from 780 to 815 nm, the generated mid-infrared signal covers the 2.9 to 3.5 µm range while optimizing the phase-match condition of the difference frequency generation process.

Synchronous mode-locking of solid-state lasers by difference frequency generation.

This Letter introduces a novel, to the best of our knowledge, method for achieving mode-locking and synchronization of mode-locked output pulses from two lasers. The proposed technique leverages parametric gain from difference frequency generation. Specifically, a Nd:YAG laser is mode-locked by single-pass mode-locked pulses from a mode-locked Ti:sapphire laser using an intracavity nonlinear crystal. When the continuous-wave laser is not actively pumped, the system functions as a synchronously pumped optical parametric oscillator. This novel approach has the potential to enable new devices, especially for pump-probe applications or for generation of mode-locked pulses in spectral regions where conventional mode-locked devices are typically not available.

Probing cortical excitability using rapid frequency tagging.

Frequency tagging has been widely used to study the role of visual selective attention. Presenting a visual stimulus flickering at a specific frequency generates so-called steady-state visually evoked responses. However, frequency tagging is mostly done at lower frequencies (<30 Hz). This produces a visible flicker, potentially interfering with both perception and neuronal oscillations in the theta, alpha and beta band. To overcome these problems, we used a newly developed projector with a 1440 Hz refresh rate allowing for frequency tagging at higher frequencies. We asked participants to perform a cued spatial attention task in which imperative pictorial stimuli were presented at 63 Hz or 78 Hz while measuring whole-head magnetoencephalography (MEG). We found posterior sensors to show a strong response at the tagged frequency. Importantly, this response was enhanced by spatial attention. Furthermore, we reproduced the typical modulations of alpha band oscillations, i.e., decrease in the alpha power contralateral to the attentional cue. The decrease in alpha power and increase in frequency tagged signal with attention correlated over subjects. We hereby provide proof-of-principle for the use of high-frequency tagging to study sensory processing and neuronal excitability associated with attention.

Coherent combining of high brightness tapered amplifiers for efficient non-linear conversion.

We report on a coherent beam combination of three high-brightness tapered amplifiers, which are seeded by a single-frequency laser at λ = 976 nm in a simple architecture with efficiently cooled emitters. The maximal combined power of 12.9 W is achieved at a combining efficiency of > 65%, which is limited by the amplifiers' intrinsic beam quality. The coherent combination cleans up the spatial profile, as the central lobe's power content increases by up to 86%. This high-brightness infrared beam is converted into the visible by second harmonic generation. This results in a high non-linear conversion efficiency of 4.5%/W and a maximum power over 2 W at 488 nm, which is limited by thermal effects in the periodically poled lithium niobate (PPLN).

Dispersion of Echinococcus granulosus eggs from infected dogs under natural conditions in Patagonia, Argentina.

Cystic echinococcosis caused by Echinococcus granulosus is a major zoonosis of public health significance in the Patagonian region of Argentina. This investigation sought to test the hypothesis that the persistence and dispersion of the parasite eggs can be explained by physical and meteorological parameters along with final host infection and behaviour. This observational study was carried out over a five-year period within an enclosure where two dogs harbouring a worm burden ranging from 100 to 1000 mature adult E. granulosus, as well as two uninfected dogs, had previously been kept for six months. Environmental canine faeces, topsoil, pond water, and sediment samples were examined to control for the presence of eggs and coproantigens of the parasite using microscope-based techniques and copro-ELISA plus copro-Western Blot tests. The parasite eggs were detected up to 41 months later in faeces from infected dogs, soil and sediment, and coproantigen tests remained positive for up to 70 months in faeces. Overall, parasite eggs were found within a maximum distance of 115 m from the contaminated dog faeces deposition site. Our findings indicate that under Patagonian environmental conditions, egg persistence and dispersion seem to be related to the worm burden and habits of the infected dog, to prevailing wind direction and to the existence of low bushes as well as natural bodies of water. The present study is the first to provide direct evidence of interaction between bioclimatic conditions and E. granulosus egg dispersion under Patagonian field conditions.

Diminished modulation of preparatory sensorimotor mu rhythm predicts attention-deficit/hyperactivity disorder severity.

BACKGROUND: Attention-deficit/hyperactivity disorder (ADHD) is characterized by problems in regulating attention and in suppressing disruptive motor activity, i.e. hyperactivity and impulsivity. We recently found evidence that aberrant distribution of posterior α band oscillations (8-12 Hz) is associated with attentional problems in ADHD. The sensorimotor cortex also produces strong 8-12 Hz band oscillations, namely the µ rhythm, and is thought to have a similar inhibitory function. Here, we now investigate whether problems in distributing α band oscillations in ADHD generalize to the µ rhythm in the sensorimotor domain. METHOD: In a group of adult ADHD (n = 17) and healthy control subjects (n = 18; aged 21-40 years) oscillatory brain activity was recorded using magnetoencephalography during a visuo-spatial attention task. Subjects had to anticipate a target with unpredictable timing and respond by pressing a button. RESULTS: Preparing a motor response, the ADHD group failed to increase hemispheric µ lateralization with relatively higher µ power in sensorimotor regions not engaged in the task, as the controls did (F 1,33 = 8.70, p = 0.006). Moreover, the ADHD group pre-response µ lateralization not only correlated positively with accuracy (r s = 0.64, p = 0.0052) and negatively with intra-individual reaction time variability (r s = -0.52, p = 0.033), but it also correlated negatively with the score on an ADHD rating scale (r s = -0.53, p = 0.028). CONCLUSIONS: We suggest that ADHD is associated with an inability to sufficiently inhibit task-irrelevant sensorimotor areas by means of modulating µ oscillatory activity. This could explain disruptive motor activity in ADHD. These results provide further evidence that impaired modulation of α band oscillations is involved in the pathogenesis of ADHD.

Decoding of task-relevant and task-irrelevant intracranial EEG representations.

Natural stimuli consist of multiple properties. However, not all of these properties are equally relevant in a given situation. In this study, we applied multivariate classification algorithms to intracranial electroencephalography data of human epilepsy patients performing an auditory Stroop task. This allowed us to identify neuronal representations of task-relevant and irrelevant pitch and semantic information of spoken words in a subset of patients. When properties were relevant, representations could be detected after about 350ms after stimulus onset. When irrelevant, the association with gamma power differed for these properties. Patients with more reliable representations of irrelevant pitch showed increased gamma band activity (35-64Hz), suggesting that attentional resources allow an increase in gamma power in some but not all patients. This effect was not observed for irrelevant semantics, possibly because the more automatic processing of this property allowed for less variation in free resources. Processing of different properties of the same stimulus seems therefore to be dependent on the characteristics of the property.

Serology and longevity of immunity against Echinococcus granulosus in sheep and llama induced by an oil-based EG95 vaccine.

An oil-based formulation of the EG95 vaccine to protect grazing animals against infection with Echinococcus granulosus was formulated in Argentina. The efficacy of the vaccine was monitored by serology in sheep and llama (Lama glama) and was compared to the serology in sheep previously published using a QuilA-adjuvanted vaccine. Long-term efficacy was also tested in sheep by challenging with E. granulosus eggs of the G1 strain 4 years after the beginning of the trial. The serological results for both sheep and llama were similar to those described previously, except that there was a more rapid response after the first vaccination. A third vaccination given after 1 year resulted in a transient boost in serology that lasted for about 12 months, which was similar to results previously described. Sheep challenged after 4 years with three vaccinations presented 84·2% reduction of live cysts counts compared with control group, and after a fourth vaccination prior to challenge, this reduction was 94·7%. The oil-based vaccine appeared to be bio-equivalent to the QuilA vaccine.

Efficient generation of 1.9 W yellow light by cascaded frequency doubling of a distributed Bragg reflector tapered diode.

Watt-level yellow emitting lasers are interesting for medical applications, due to their high hemoglobin absorption, and for efficient detection of certain fluorophores. In this paper, we demonstrate a compact and robust diode-based laser system in the yellow spectral range. The system generates 1.9 W of single-frequency light at 562.4 nm by cascaded single-pass frequency doubling of the 1124.8 nm emission from a distributed Bragg reflector (DBR) tapered laser diode. The absence of a free-space cavity makes the system stable over a base-plate temperature range of 30 K. At the same time, the use of a laser diode enables the modulation of the pump wavelength by controlling the drive current. This is utilized to achieve a power modulation depth above 90% for the second harmonic light, with a rise time below 40 µs.

Concept for power scaling second harmonic generation using a cascade of nonlinear crystals.

Within the field of high-power second harmonic generation (SHG), power scaling is often hindered by adverse crystal effects such as thermal dephasing arising from the second harmonic (SH) light, which imposes limits on the power that can be generated in many crystals. Here we demonstrate a concept for efficient power scaling of single-pass SHG beyond such limits using a cascade of nonlinear crystals, in which the first crystal is chosen for high nonlinear efficiency and the subsequent crystal(s) are chosen for power handling ability. Using this highly efficient single-pass concept, we generate 3.7 W of continuous-wave diffraction-limited (M(2)=1.25) light at 532 nm from 9.5 W of non-diffraction-limited (M(2)=7.7) light from a tapered laser diode, while avoiding significant thermal effects. Besides constituting the highest SH power yet achieved using a laser diode, this demonstrates that the concept successfully combines the high efficiency of the first stage with the good power handling properties of the subsequent stages. The concept is generally applicable and can be expanded with more stages to obtain even higher efficiency, and extends also to other combinations of nonlinear media suitable for other wavelengths.

Environmental factors related to the abundance and activity of Ochlerotatus albifasciatus (Diptera: Culicidae) in an agricultural landscape of steppe arid climate.

Ochlerotatus albifasciatus (Macquart) is a flood water mosquito whose highest density has been found associated both with natural landscapes (prairies or grazing fields) in temperate and subtropical regions and with rainfall events. In the current study, we aimed to find out how the marked differences between environmental factors of agricultural landscape patches in a steppe arid region affect the relative abundance of this species. In wetland patches, the high activity of adults was closely associated with the flood irrigation system, suggesting that the agricultural activity contributes to the proliferation of this mosquito. The steppe patches would constitute an adverse environment reflected by the abrupt decrease in abundance. Multiple linear regression showed that some explanatory variables, such as wetland patches and moment of the day (midday), did not contribute significantly to the relative abundance variation. In contrast, temperature, wind, and cloud cover seemed to regulate the biting activity of females. Temperature affected the activity of mosquitoes in the steppe but seemed to have no effect in wetland patches, where the activity of mosquitoes was permanent and more stable against changes in temperature. In the steppe, which presents low levels of humidity, scarce vegetation, and greater wind exposure, the activity seemed to be unstable against small thermal variations. The variability of the relative abundance of Oc. albifasciatus in an agricultural landscape was widely explained by temperature in combination with the microenvironment type, wind speed, and cloud cover and indirectly by human activity.

Reorganization of oscillatory activity in human parietal cortex during spatial updating.

Single-neuron recordings have shown that the posterior parietal cortex (PPC) processes spatial information in many frames of reference, including gaze-centered, head-centered, body-centered, and intermediate coding frames. At the population level, rhythmic neuronal synchronization may provide a mechanism by which PPC could selectively emphasize the task-relevant reference frame in spatial processing. Using magnetoencephalography, we tested this hypothesis by studying the modulations in oscillatory activity in a spatial updating task. Human subjects had to remember the location of a target, briefly flashed left or right of central fixation. Next, they refixated and then, after a further memory delay, made a saccade to the memorized target location. We observed gamma-band (>40 Hz) synchronization and alpha-band (8-12 Hz) desychronization in contralateral occipital and parietal areas, both showing updating in a gaze-centered reference frame but with fast and slow time courses, respectively. Furthermore, after updating, ipsilateral areas showed less alpha desynchronization when they had been contralateral to the target before updating. Taken together, our results suggest that power in the gamma band is instantly reorganized to encode task-relevant visuomotor space in a gaze-centered reference frame, while power in the alpha band reflects a regulatory mechanism actively facilitating the gating of the saccade target and inhibiting the original stimulus representation.

'I take for granted that patients know' - oral health professionals' strategies, considerations and methods when teaching patients how to use fluoride toothpaste.

OBJECTIVES: The aim of this study was to explore the oral health professionals' (OHPs') perspectives regarding their strategies, considerations and methods when teaching their patients the most effective way of toothbrushing with fluoride (F) toothpaste. METHODS: A qualitative research method was used to collect data. To stimulate interactivity among the participants, interviews were performed in focus groups. Five groups of OHPs, including dentists, dental hygienists and dental nurses, were interviewed a total of 23 individuals. The interviews were analysed using manifest and latent qualitative content analysis. Data were systematically condensed and coded to the relevant phrases that identified their content. RESULTS: Three categories were identified in the manifest and latent content analysis: (i) strategies and intentions, (ii) providing oral hygiene information and instruction and (iii) barriers to optimal oral healthcare education. Health promotion and seeing to the patients' best interest were driving forces among the OHPs as well as personal success in their preventive work. They focused on toothbrushing techniques more than on how to use F toothpaste. Barriers to oral health information were cost to the patients and, to some extent, the opinion of the OHPs that some patients were impossible to motivate or that patients already know what to do. CONCLUSION: The OHPs described toothbrushing with F toothpaste as very important, although the plaque removal perspective dominated. They did not focus on how to use F toothpaste, because they believed that knowledge about and appropriate behaviour concerning F toothpaste were already familiar to their patients.

MEG-based decoding of the spatiotemporal dynamics of visual category perception.

Visual processing is a complex task which is best investigated using sensitive multivariate analysis methods that can capture representation-specific brain activity over both time and space. In this study, we applied a multivariate decoding algorithm to MEG data of subjects engaged in passive viewing of images of faces, scenes, bodies and tools. We used reconstructed source-space time courses as input to the algorithm in order to localize brain regions involved in optimal image discrimination. Applying this method to the interval of 115 to 315 ms after stimulus onset, we show a focal localization of regression coefficients in the inferior occipital, middle occipital, and lingual gyrus that drive decoding of the different perceived image categories. Classifier accuracy was highest (over 90% correctly classified trials, compared to a chance level accuracy of 50%) when dissociating the perception of faces from perception of other object categories. Furthermore, we applied this method to each single time point to extract the temporal evolution of visual perception. This allowed for the detection of differences in visual category perception as early as 85 ms after stimulus onset. Furthermore, localizing the corresponding regression coefficients of each time point allowed us to capture the spatiotemporal dynamics of visual category perception. This revealed initial involvement of sources in the inferior occipital, inferior temporal and superior occipital gyrus. During sustained stimulation additional sources in the anterior inferior temporal gyrus and superior parietal gyrus became involved. We conclude that decoding of source-space MEG data provides a suitable method to investigate the spatiotemporal dynamics of ongoing cognitive processing.

Simultaneous dual wavelength eye-tracked ultrahigh resolution retinal and choroidal optical coherence tomography.

We demonstrate an optical coherence tomography device that simultaneously combines different novel ultrabroad bandwidth light sources centered in the 800 and 1060 nm regions, operating at 66 kHz depth scan rate, and a confocal laser scanning ophthalmoscope-based eye tracker to permit motion-artifact-free, ultrahigh resolution and high contrast retinal and choroidal imaging. The two wavelengths of the device provide the complementary information needed for diagnosis of subtle retinal changes, while also increasing visibility of deeper-lying layers to image pathologies that include opaque media in the anterior eye segment or eyes with increased choroidal thickness.

A biomechanical model of anther opening reveals the roles of dehydration and secondary thickening.

Understanding the processes that underlie pollen release is a prime target for controlling fertility to enable selective breeding and the efficient production of hybrid crops. Pollen release requires anther opening, which involves changes in the biomechanical properties of the anther wall. In this research, we develop and use a mathematical model to understand how these biomechanical processes lead to anther opening. Our mathematical model describing the biomechanics of anther opening incorporates the bilayer structure of the mature anther wall, which comprises the outer epidermal cell layer, whose turgor pressure is related to its hydration, and the endothecial layer, whose walls contain helical secondary thickening, which resists stretching and bending. The model describes how epidermal dehydration, in association with the thickened endothecial layer, creates forces within the anther wall causing it to bend outwards, resulting in anther opening and pollen release. The model demonstrates that epidermal dehydration can drive anther opening, and suggests why endothecial secondary thickening is essential for this process (explaining the phenotypes presented in the myb26 and nst1nst2 mutants). The research hypothesizes and demonstrates a biomechanical mechanism for anther opening, which appears to be conserved in many other biological situations where tissue movement occurs.

A model of crosslink kinetics in the expanding plant cell wall: yield stress and enzyme action.

The plant primary cell wall is a composite material containing stiff cellulose microfibrils that are embedded within a pectin matrix and crosslinked through a network of hemicellulose polymers. This microstructure endows the wall with nonlinear anisotropic mechanical properties and allows enzymatic regulation of expansive cell growth. We present a mathematical model of hemicellulose crosslink dynamics in an expanding cell wall incorporating strain-enhanced breakage and enzyme-mediated crosslink kinetics. The model predicts the characteristic yielding behaviour in the relationship between stress and strain-rate seen experimentally, and suggests how the effective yield and extensibility of the wall depend on microstructural parameters and on the action of enzymes of the XTH and expansin families. The model suggests that the yielding behaviour encapsulated in the classical Lockhart equation can be explained by the strongly nonlinear dependence of crosslink breakage rate on crosslink elongation. The model also demonstrates how enzymes that target crosslink binding can be effective in softening the wall in its pre-yield state, whereas its post-yield extensibility is determined primarily by the pectin matrix.

Drones can reliably, accurately and with high levels of precision, collect large volume water samples and physio-chemical data from lakes.

The rapid development and application of drone technology has included water sampling and collection of physiochemical data from lakes. Previous research has demonstrated the significant potential of drones to play a future pivotal role in the collection of such data from lakes that fulfil requirements of large-scale monitoring programmes. However, currently the utilisation of drone technology for water quality monitoring is hindered by a number of important limitations: i) the low rate of successful sample captured; ii) the relatively low volume of water sample retrieved for analyses of multiple water chemistry parameters; and critically iii) differences between water chemistry parameters when using a drone versus samples collected by boat. Here we present results comparing the water chemistry results of a large number of parameters (pH, dissolved oxygen concentration, temperature, conductivity, alkalinity, hardness, true colour, chloride, silica, ammonia, total oxidised nitrogen, nitrite, nitrate, ortho-phosphate, total phosphorous and chlorophyll) sampled via drone with samples collected by boat in a number of lakes. The drone water sampling method used here is the first to collect a sufficiently large volume of water to meet the monitoring requirements of large scale water monitoring programmes, 2 L, at a 100% success rate and most crucially, with water chemistry variables that are not significantly different to those taken using traditional boat water sampling. This study therefore shows that drone technology can be utilised to collect water chemistry data and samples from lakes in a reliable, more rapid and cost effective manner than traditional sampling using boats, that is safer for personnel and poses less of a biosecurity risk.

Quenching of spectroscopic factors for proton removal in oxygen isotopes.

We present microscopic coupled-cluster calculations of the spectroscopic factors for proton removal from the closed-shell oxygen isotopes (14,16,22,24,28)O with a chiral nucleon-nucleon interaction at next-to-next-to-next-to-leading order. We include coupling-to-continuum degrees of freedom by using a Hartree-Fock basis built from a Woods-Saxon single-particle basis. This basis treats bound and continuum states on an equal footing. We find a significant quenching of spectroscopic factors in the neutron-rich oxygen isotopes, pointing to enhanced many-body correlations induced by strong coupling to the scattering continuum above the neutron emission thresholds.