RESUMEN
OBJECTIVE: To evaluate the clinical use of Homocysteine-Primavette, a new blood collection medium for total homocysteine (tHcy) assay. METHODS: The agreement between baseline tHcy and tHcy in stabilized samples (40 h) was assessed for FPIA, HPLC, GC-MS, LC-MS, and ICL. RESULTS: tHcy concentrations in whole blood were stable for 40 h in Hcy-Primavette tubes. CONCLUSION: Primavette tubes are a good alternative for the accurate tHcy measurement and no readjustment of reference intervals is needed.
Asunto(s)
Recolección de Muestras de Sangre/instrumentación , Homocisteína/sangre , Cromatografía Líquida de Alta Presión , Cromatografía Liquida , Ácido Edético/farmacología , Inmunoensayo de Polarización Fluorescente , Cromatografía de Gases y Espectrometría de Masas , Humanos , Mediciones Luminiscentes , Espectrometría de MasasRESUMEN
A new ß variant was found in a German diabetic patient whose blood samples appeared to contain 45% Hb A(1c) using Bio-Rad Variant V-II A1c-analyzer but 7.6% on boronate affinity chromatography. Structural studies using, HPLC, mass spectrometry, and the genomic DNA analysis revealed a new substitution in which the cysteine residue at position ß93 was replaced by serine. The variant was named Hb Riesa or ß93 (F9) CysâSer and accounted for 54.3% of the total haemoglobin. This suggests that the protein-synthesis processes for the mutant could be slightly more promoted than those of the wild-type. Hb Riesa is clinically and electrophoretically silent.