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1.
Nat Med ; 6(2): 207-10, 2000 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-10655111

RESUMEN

The development of the human immunodeficiency virus-1 (HIV-1)/simian immunodeficiency virus (SIV) chimeric virus macaque model (SHIV) permits the in vivo evaluation of anti-HIV-1 envelope glycoprotein immune responses. Using this model, others, and we have shown that passively infused antibody can protect against an intravenous challenge. However, HIV-1 is most often transmitted across mucosal surfaces and the intravenous challenge model may not accurately predict the role of antibody in protection against mucosal exposure. After controlling the macaque estrous cycle with progesterone, anti-HIV-1 neutralizing monoclonal antibodies 2F5 and 2G12, and HIV immune globulin were tested. Whereas all five control monkeys displayed high plasma viremia and rapid CD4 cell decline, 14 antibody-treated macaques were either completely protected against infection or against pathogenic manifestations of SHIV-infection. Infusion of all three antibodies together provided the greatest amount of protection, but a single monoclonal antibody, with modest virus neutralizing activity, was also protective. Compared with our previous intravenous challenge study with the same virus and antibodies, the data indicated that greater protection was achieved after vaginal challenge. This study demonstrates that antibodies can affect transmission and subsequent disease course after vaginal SHIV-challenge; the data begin to define the type of antibody response that could play a role in protection against mucosal transmission of HIV-1.


Asunto(s)
Anticuerpos Monoclonales/administración & dosificación , VIH-1/inmunología , Síndrome de Inmunodeficiencia Adquirida del Simio/prevención & control , Virus de la Inmunodeficiencia de los Simios/inmunología , Vagina/inmunología , Animales , Quimera , Femenino , Anticuerpos Anti-VIH/análisis , Anticuerpos Anti-VIH/sangre , VIH-1/genética , Inmunidad Mucosa , Inmunización Pasiva , Macaca mulatta , Pruebas de Neutralización , Síndrome de Inmunodeficiencia Adquirida del Simio/inmunología , Síndrome de Inmunodeficiencia Adquirida del Simio/transmisión , Virus de la Inmunodeficiencia de los Simios/genética
2.
Nat Med ; 6(2): 200-6, 2000 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-10655110

RESUMEN

Although maternal human immunodeficiency virus type 1 (HIV-1) transmission occurs during gestation, intrapartum and postpartum (by breast-feeding), 50-70% of all infected children seem to acquire HIV-1 shortly before or during delivery. Epidemiological evidence indicates that mucosal exposure is an important aspect of intrapartum HIV transmission. A simian immunodeficiency virus (SIV) macaque model has been developed that mimics the mucosal exposure that can occur during intrapartum HIV-1 transmission. To develop immunoprophylaxis against intrapartum HIV-1 transmission, we used SHIV-vpu+ (refs. 5,6), a chimeric simian-human virus that encodes the env gene of HIV-IIIB. Several combinations of human monoclonal antibodies against HIV-1 have been identified that neutralize SHIV-vpu+ completely in vitro through synergistic interaction. Here, we treated four pregnant macaques with a triple combination of the human IgG1 monoclonal antibodies F105, 2G12 and 2F5. All four macaques were protected against intravenous SHIV-vpu+ challenge after delivery. The infants received monoclonal antibodies after birth and were challenged orally with SHIV-vpu+ shortly thereafter. We found no evidence of infection in any infant during 6 months of follow-up. This demonstrates that IgG1 monoclonal antibodies protect against mucosal lentivirus challenge in neonates. We conclude that epitopes recognized by the three monoclonal antibodies are important determinants for achieving substantial protection, thus providing a rational basis for AIDS vaccine development.


Asunto(s)
Anticuerpos Monoclonales/inmunología , VIH-1/inmunología , Inmunidad Mucosa , Inmunoglobulina G/inmunología , Síndrome de Inmunodeficiencia Adquirida del Simio/prevención & control , Virus de la Inmunodeficiencia de los Simios/inmunología , Animales , Quimera , Femenino , VIH-1/genética , Transmisión Vertical de Enfermedad Infecciosa , Macaca mulatta , Pruebas de Neutralización , Embarazo , Complicaciones Infecciosas del Embarazo , Síndrome de Inmunodeficiencia Adquirida del Simio/inmunología , Síndrome de Inmunodeficiencia Adquirida del Simio/transmisión , Virus de la Inmunodeficiencia de los Simios/genética
3.
Tuberculosis (Edinb) ; 86(3-4): 236-46, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16677861

RESUMEN

We generated several attenuated recombinant influenza A vectors expressing the Mycobacterium tuberculosis early secretory antigenic target (ESAT-6) protein. The ESAT-6 protein was recently identified as one of the most promising protective antigens for cell-mediated immunity. The obtained vectors appeared to be capable of inducing ESAT-6 specific Th1 immune response in mice after intranasal immunization. We found that double immunization with two influenza vectors of different subtypes provided a significant level of protection in mice, when applied as prophylactic vaccine, as well as substantial therapeutic effect in mice with pre-established tuberculosis infection. Moreover, we found a strong synergistic effect when vaccination with Flu/ESAT-6 vectors was combined with isoniazid treatment, resulting in a dramatic reduction of bacterial load in the lungs of infected mice.


Asunto(s)
Antígenos Bacterianos/inmunología , Proteínas Bacterianas/inmunología , Vacunas contra la Tuberculosis/inmunología , Tuberculosis/prevención & control , Administración Intranasal , Animales , Antígenos Bacterianos/genética , Antígenos Bacterianos/uso terapéutico , Antituberculosos/uso terapéutico , Proteínas Bacterianas/genética , Proteínas Bacterianas/uso terapéutico , Terapia Combinada , Vectores Genéticos/administración & dosificación , Inmunidad Celular , Inmunización/métodos , Virus de la Influenza A/genética , Isoniazida/uso terapéutico , Ratones , Ratones Endogámicos C57BL , Mycobacterium tuberculosis/inmunología , Células TH1/inmunología , Tuberculosis/inmunología , Tuberculosis/terapia , Vacunas contra la Tuberculosis/uso terapéutico
4.
Int J Oncol ; 26(4): 961-70, 2005 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-15753990

RESUMEN

Establishment of tumor cell lines as model systems for studying tumor biology or as a part of immunotherapeutic anti-cancer strategies is of high importance, whereby the highest possible preservation of the original tumor cell phenotype is a prerequisite for these aims. Since overexpression of the catalytic subunit of human telomerase (hTERT) is known to minimally alter the cellular phenotype, we focused on the establishment of cell lines derived from human fibroma from a MEN1 patient by ectopic expression of hTERT. Additionally, a cell line was generated by introduction of the early region of SV40 (SV40 ER). Both approaches resulted in continuous cell lines, and neither T1-LOHG (hTERT) nor SV1-LOHG (SV40 ER) showed a transformed phenotype. While SV40 ER-transfected cells underwent dramatic changes in morphology and growth characteristics, hTERT-expressing cells indeed retained a phenotype highly similar to the parental cells. Nevertheless, hTERT overexpression resulted in increased growth rates after about 70 population doublings (PD) and alterations of mRNA levels of genes associated with tumor pathogenesis. Thus, our data suggest that ectopic hTERT expression leads to immortalization of LOHG-F, sustaining many characteristics of the non-transfected counterparts, but continuous growth in vitro is associated with changes of the cellular phenotype.


Asunto(s)
Antígenos Transformadores de Poliomavirus/inmunología , Proliferación Celular , Fibroma/genética , Fibroma/patología , Neoplasia Endocrina Múltiple Tipo 1/genética , Neoplasia Endocrina Múltiple Tipo 1/patología , Telomerasa/biosíntesis , Telomerasa/farmacología , Células Tumorales Cultivadas , Supervivencia Celular , Proteínas de Unión al ADN , Humanos , Fenotipo , ARN Mensajero/biosíntesis , Transfección
5.
Mol Immunol ; 30(13): 1159-63, 1993 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-8413320

RESUMEN

Based on our findings that HIV-1 gp41 independently of CD4, can bind to the helper T-cell line H9, we characterized putative binding of HIV-1 gp41 to B-cell lines, Raji, Bjab and Ramos. Using fluorescence-activated cell sorter (FACS) we examined the binding of soluble gp41 (sgp41; Env amino acid 539-684) to these B-cell lines. Using sgp41 attached to sepharose beads Raji cell lysates were absorbed. The sgp41-eluate of Raji cell lysates could inhibit the sgp41-binding to Raji cells. By SDS-PAGE of sgp41-eluate of Raji cell lysates four strong protein band, 37, 45, 49 and 62 kD, and a weak band of 92 kD were stained with Coomassie blue. By Western blot (ligand blot) analysis using sgp41 four protein bands, 37, 45, 49 and 62 kD, were observed in sgp41-eluate of Raji cell lysates. To test the individual proteins the five proteins were isolated from the sgp41-eluate of Raji cell lysates. Three proteins, 45, 49 and 62 kD, each could partially inhibit the sgp41-binding to Raji cells. The results suggest that these three proteins in Raji cell lysates are possible candidates for the putative gp41 receptor(s).


Asunto(s)
Linfocitos B/metabolismo , Proteína gp41 de Envoltorio del VIH/metabolismo , VIH-1/metabolismo , Receptores del VIH/metabolismo , Linfocitos B/química , Unión Competitiva , Línea Celular , Cromatografía de Afinidad , Humanos , Fragmentos de Péptidos/metabolismo , Proteínas Recombinantes/metabolismo , Fracciones Subcelulares/metabolismo
6.
Mol Immunol ; 31(13): 977-82, 1994 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-8084338

RESUMEN

Based on our findings, that HIV-1 soluble gp41 could bind to several proteins on the human, T, B and monocyte cells independently of CD4, we examined the effect of HIV-1 soluble gp41 (sgp41;Env amino acids 539-684) on surface expression of MHC I and II, ICAM-1 and CD21 molecules on human Raji cells. Flow cytometry (FACS) analysis demonstrated that sgp41 could selectively enhance MHC class I and II expression on Raji cells, but did not increase expression of other cell surface antigens, such as, CD21 and CD54 (ICAM-1). Soluble gp41 could also enhance MHC class I and II expression on another human B cell line, Bjab. The sgp41-dependent enhancement of the MHC class I and II expression on Raji cells is time- and dose-dependent. The sgp41 enhancement effect on the MHC antigen expression could be inhibited by the sgp41-binding proteins of 45, 49 and 62 kD (isolated from Raji-lysate) which could inhibit the spg41-binding to Raji cells. Interestingly, this sgp41-dependent enhancement of the MHC class I and II expression could also be inhibited by two mAbs to HIV-1 gp41, but not by a third mAb binding to a different site on gp41. These results demonstrate that HIV-1 sgp41 can selectively enhance the human Raji cell MHC class I and II antigen expression and this enhancement effect could be inhibited by the sgp41-binding proteins and anti-gp41 antibodies, and suggest that the sgp41-dependent enhancement is mediated by its binding to Raji membrane proteins of 45, 49 and 62 kD.


Asunto(s)
Proteína gp41 de Envoltorio del VIH/inmunología , VIH-1/inmunología , Antígenos HLA-D/biosíntesis , Antígenos de Histocompatibilidad Clase I/biosíntesis , Anticuerpos Monoclonales/inmunología , Proteínas Portadoras/inmunología , Citometría de Flujo , Anticuerpos Anti-VIH/inmunología , Humanos , Células Tumorales Cultivadas
7.
Mol Immunol ; 30(17): 1583-6, 1993 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-8247028

RESUMEN

Based on our finding that HIV-1 gp41 independently of CD4 can bind to several proteins (gp41 binding protein: GBP) on the human T-cell line H9, B-cell line Raji and monocyte-cell line U937, we examined the effect of mitogens and cytokines on binding of gp41 to H9, Raji and U937 cells. Flow cytometry (FACS) analysis demonstrated that PWM and LPS, IFN-gamma and IL-6, but not Con A, IFN-alpha, -beta, -omega and IL-2, could increase gp41 binding to Raji cells. In controls, none of the regulators (IFN-alpha, -beta, -gamma, -omega, IL-2, IL-6, Con A, PWM, LPS) could modify the binding potential of H9 and U937 cells. Our data suggest that the expression of HIV-1 binding proteins is subject to regulation by PWM, LPS, IFN-gamma and IL-6 in the case of B-cells, while on T-cells and macrophages, the binding proteins may be constitutively expressed.


Asunto(s)
Proteína gp41 de Envoltorio del VIH/metabolismo , Interferón gamma/farmacología , Interleucina-6/farmacología , Lipopolisacáridos/farmacología , Linfocitos/inmunología , Mitógenos de Phytolacca americana/farmacología , Linfocitos B/efectos de los fármacos , Linfocitos B/inmunología , Linfocitos B/metabolismo , Línea Celular , Proteína gp41 de Envoltorio del VIH/efectos de los fármacos , Proteína gp41 de Envoltorio del VIH/inmunología , Humanos , Linfocitos/efectos de los fármacos , Linfocitos/metabolismo , Monocitos/efectos de los fármacos , Monocitos/inmunología , Monocitos/metabolismo , Unión Proteica/efectos de los fármacos , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunología , Linfocitos T/metabolismo
8.
Mol Immunol ; 34(12-13): 855-63, 1997.
Artículo en Inglés | MEDLINE | ID: mdl-9464521

RESUMEN

Adhesion molecules are known to contribute to infectivity of HIV-1. Here we tested whether the complement receptor type 3 (CR3, CD11b), an alpha(m)beta2 integrin, plays an accessory role in the infection process of HIV-1, because ICAM-1, a ligand of CR3, is present on the envelope of HIV-1. In addition, the viral transmembrane protein gp41 shares four regions of homology with the complement component C3, a further CR3 ligand. Infection of PBMCs with HIV-IIIB and primary isolates was partially inhibited by anti-CR3 antibodies. A peptide derived from the complement component C3, covering the CR3-binding site of C3 and sharing strong similarity to the immunosuppressive region of gp41, significantly reduced the HIV-1 titer in infection assays. Recombinant soluble gp41 (rsgp41) and the peptide covering the immunosuppressive domain of gp41 inhibited the rosetting of iC3b-coated sheep erythrocytes with U937 via complement receptors (CRs) with an efficiency comparable to monoclonal anti-CR antibodies. In addition, sub-populations of CD4 + and CD8 + T-cells isolated from HIV-infected individuals were found to upregulate CR3 as determined by FACS analysis and on the mRNA level. Since gp41 has been implicated in viral fusion, an interaction of its C3-homology region in gp41 or an interaction of ICAM on the surface of free virus with CRs might contribute to facilitate viral entry.


Asunto(s)
Anticuerpos Monoclonales/uso terapéutico , Infecciones por VIH/prevención & control , VIH-1 , Proteínas de la Membrana , Receptores de Complemento/inmunología , Fármacos Anti-VIH/uso terapéutico , Anticuerpos Monoclonales/inmunología , Linfocitos T CD4-Positivos/citología , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD8-positivos/citología , Linfocitos T CD8-positivos/inmunología , Separación Celular , Citometría de Flujo , VIH-1/metabolismo , VIH-1/patogenicidad , Humanos , Inmunización Pasiva , Técnicas In Vitro , Leucocitos Mononucleares/inmunología , Leucocitos Mononucleares/virología , Antígeno de Macrófago-1/metabolismo , ARN Mensajero/análisis , Receptores de Complemento/genética , Formación de Roseta
9.
AIDS ; 10(14): 1611-20, 1996 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-8970680

RESUMEN

OBJECTIVE: To determine the acquisition of host cell-membrane-derived molecules by HIV-1 during the budding process, and to investigate whether the uptake of these molecules is cell-type-specific and selective. DESIGN: Virions, propagated by four different cell types were analysed for the presence of adhesion molecules, glycosylphosphatidylinositol (GPI)-anchored proteins and various cell-surface markers. The pattern was compared with the phenotype of the HIV-1-infected cell. METHODS: For phenotypic analysis of virions a two-step assay was used. In the first step, virus was captured with monoclonal antibodies (in some cases polyclonal sera) against different cell-membrane proteins. In a second step, the presence of virus was measured by determining the concentration of the virus-specific p24 core antigen. The expression of surface molecules on uninfected and HIV-1IIIB-infected cells was analysed by FACS. RESULTS: Depending on the cell type used for virus propagation, different cell-membrane molecules were found on the virus surface reflecting the corresponding cell type. The uptake of these molecules was selective to a certain degree. No CD4 and CD87 molecules were detectable on HIV-1, although both molecules were present on uninfected and HIV-1-infected cells. CR3 and CDw108 could not be seen on uninfected cells, but wre detectable on infected cells and virions. CONCLUSIONS: During the budding process HIV-1 acquires a variety of cell-type-specific cell-surface molecules. Certain cell-membrane molecules become upregulated during HIV-1-infection and are then found on virions, whereas other molecules remain on the cell surface and do not become incorporated.


Asunto(s)
Membrana Celular/virología , Infecciones por VIH/metabolismo , VIH-1 , Proteínas de la Membrana/análisis , Línea Celular , Membrana Celular/metabolismo , Citometría de Flujo , Regulación Viral de la Expresión Génica , Humanos , Proteínas de la Membrana/biosíntesis
10.
AIDS ; 10(6): 587-93, 1996 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-8780812

RESUMEN

OBJECTIVE: To investigate whether variations of the conserved gp41 amino-acid sequence ELDKWA affect its binding or neutralization by monoclonal antibody (MAb) 2F5. DESIGN AND METHODS: Neutralization assays were performed with primary isolates from different HIV-1 subtypes and the sequences corresponding to the 2F5 epitope region were analysed. Studies of MAb 2F5 peptide reactivity were performed by spot analysis, using peptides immobilized on cellulose. The frequency of emergence of neutralization-resistant virus variants was determined by immune selection experiments in the presence of MAb 2F5. RESULTS: Primary isolates from clades A, B and E were neutralized by MAb 2F5. Neutralization sensitivity correlated with the presence of the LDKW motif. A K-to-N change in the core sequence was identified in a neutralization-resistant patient isolate. Neutralization resistant virus variants that were selected in the presence of MAb 2F5 were found to contain D-to-N, D-to-E, or K-to-N changes within the LDKW sequence. Neither in natural isolates nor in variants obtained under immune selection conditions in the laboratory were changes in the L and W positions observed. Studies of MAb 2F5 binding to variations of the ELDKWA peptide confirmed that the changes at the first and last positions did not significantly reduce binding capacity, whereas amino-acid changes from D to N, D to E, and K to N almost completely abrogated binding of MAb 2F5. CONCLUSION: Sequence analysis of a variety of primary isolates suggests that the major determinant of MAb 2F5 binding corresponds to the amino-acid sequence LDKW. Naturally occurring and in vitro selected neutralization-resistant viruses contained changes in the D and K positions of the ELDKWA motif.


Asunto(s)
Variación Antigénica , Proteína gp41 de Envoltorio del VIH/inmunología , VIH-1/inmunología , Anticuerpos Monoclonales/inmunología , Humanos , Análisis de Secuencia
11.
AIDS ; 6(6): 533-9, 1992 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-1388873

RESUMEN

OBJECTIVE: To characterize putative binding sites for HIV-1 gp41 to H9 cells. DESIGN: Based on accumulating evidence in the literature that HIV-1 can bind to cell surfaces independent of CD4, we attempted to clarify whether gp41, the transmembrane HIV-1 protein, contributes to CD4-independent binding. We therefore tested binding of gp41 to cells. METHODS: Using fluorescence-activated cell-sorter analysis, we examined the binding of recombinant gp160 (gp160) and soluble gp41 (sgp41; Env amino acids 539-684) to H9 cells, and located the putative binding site(s) of gp41 by inhibition using 16 HIV-1 Env peptides. Putative HIV-1 receptor proteins in H9 cell lysates were Western blotted and precipitated using sgp41. RESULTS: sgp41 bound to the CD4+ H9 cells and rgp160 bound to H9 cells independent of gp120-binding sites on CD4 molecules. Two gp41 peptides (Env amino acids 591-605 and 651-665) inhibited the binding of sgp41 to H9 cells. Four bands, of 37, 40, 55 and 97 kD, were blotted in H9 cell lysates, and three bands, 40, 97 and 108 kD, were observed in the precipitation analysis using lysates of 125I-surface-labelled H9 cells and sgp41 attached to sepharose beads. CONCLUSIONS: HIV-1 gp41 contains two putative binding sites to H9 cells. These sites may be located within Env amino acids 591-605 (ERYLKDQQLLGIWGC) and 651-665 (TLLEESQNQQEKNEQ). Using two different techniques, five proteins (37, 40, 55, 97 and 108 kD) were identified in H9 lysates as possible candidates for gp41 binding.


Asunto(s)
Proteína gp41 de Envoltorio del VIH/metabolismo , VIH-1/metabolismo , Proteínas de la Membrana/metabolismo , Linfocitos T Colaboradores-Inductores/microbiología , Secuencia de Aminoácidos , Sitios de Unión , Antígenos CD4/metabolismo , Línea Celular , Productos del Gen env/metabolismo , Proteína gp120 de Envoltorio del VIH/metabolismo , Proteínas gp160 de Envoltorio del VIH , Proteína gp41 de Envoltorio del VIH/química , Humanos , Proteínas de la Membrana/aislamiento & purificación , Datos de Secuencia Molecular , Precursores de Proteínas/metabolismo , Receptores del VIH/química , Receptores del VIH/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Linfocitos T Colaboradores-Inductores/metabolismo
12.
AIDS ; 7(7): 903-10, 1993 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-7689323

RESUMEN

OBJECTIVE: To analyse the ability of different HIV-1 and HIV-2 isolates to activate the complement system. DESIGN: H9 cells chronically infected with various HIV isolates and the corresponding purified viruses were tested for complement activation. To identify the molecules responsible for complement activation on the surface of infected cells, the expression of complement inhibitors/regulators and viral proteins on the cell surface was analysed. METHODS: C3 deposition on the cell surface and the expression of viral and cellular antigens were determined by flow cytometry analysis. Complement activation by purified viruses was measured using a complement consumption assay and a C1 activation assay. RESULTS: H9 cells infected with different HIV-1 and HIV-2 isolates showed varying degrees of complement activation on the cell surface, ranging from strong activation and deposition of large amounts of C3 to no increased C3 deposition compared to uninfected cells. The C3 deposition was eliminated by EDTA and reduced in the presence of EGTA. In contrast, all purified viral isolates tested activated the complement system in a comparable manner. While the expression of MCP, DAF and CD59 was not modified after infection with different viral isolates, the reaction of the infected cells with a monoclonal antibody (3D6) directed against a gp41 epitope (amino acids 601-620) was found to correlate with the complement activation on the cell surface. CONCLUSIONS: Some HIV-1 as well as HIV-2 isolates activate the complement system on the surface of infected cells independent of anti-HIV antibodies, while other isolates fail to do so. Complement activation on the cell surface is mediated by the alternative and, to a lesser extent, the classical pathway. The differences in complement activation on the cell surface are not caused by a modified expression of membrane-bound complement inhibitors/regulators. C3 deposition on the cell surface correlates with the expression of an epitope lying within the major complement activating domain of gp41 (amino acids 591-620). These results suggest a role of gp41 for complement activation on HIV-infected cells as has been described previously for purified HIV.


Asunto(s)
Activación de Complemento , VIH-1/inmunología , VIH-2/inmunología , Antígenos CD/análisis , Antígenos CD55 , Antígenos CD59 , Membrana Celular/metabolismo , Células Cultivadas , Complemento C3/metabolismo , Proteínas Inactivadoras de Complemento/análisis , Ensayo de Inmunoadsorción Enzimática , Técnica del Anticuerpo Fluorescente , Variación Genética , Proteína gp41 de Envoltorio del VIH/análisis , Humanos , Leucocitos Mononucleares/citología , Proteína Cofactora de Membrana , Glicoproteínas de Membrana/análisis
13.
J Immunol Methods ; 235(1-2): 61-9, 2000 Feb 21.
Artículo en Inglés | MEDLINE | ID: mdl-10675758

RESUMEN

Immunoassays designed to measure low concentrations of staphylococcal protein A (SPA) that have been leached into antibody preparations intended for therapeutic use are subject to differing degrees of interference. Methods established to quantify SPA in murine antibody preparations are not accurate in the presence of human or humanized IgG. We report the development of an enzyme-linked immunosorbent assay (ELISA) for SPA with a detection limit of 7 pg/ml and the optimization of a method that permits complete dissociation of SPA-immunoglobulin-complexes. This assay is a modification of our heat-mediated dissociation (HD-SD) treatment with sodium dodecyl sulfate (SDS) and diethylenetriaminepentacetic acid (DTPA) for total immune-complex dissociation, in which the heat treatment has been prolonged and the diluent is characterized by increased protein content and buffering capacity. The diluent developed contains SDS, DTPA and bovine serum albumin dissolved in a 0.1 M phosphate buffer (pH 7.2). To validate the efficiency of this novel method, a series of samples have been assayed, including samples reconstituted in vitro, samples of purified antibodies, and plasma from patients. The described method has been shown to be generally efficient in quantitating all native and recombinant SPA in samples containing up to 50 mg/ml of human IgG. These data demonstrate the utility of this technique in determining SPA contamination of recombinant immunoglobulin therapeutic products.


Asunto(s)
Ensayo de Inmunoadsorción Enzimática/métodos , Proteína Estafilocócica A/análisis , Anticuerpos/química , Contaminación de Medicamentos , Humanos , Sensibilidad y Especificidad , Proteína Estafilocócica A/sangre
14.
J Immunol Methods ; 217(1-2): 143-51, 1998 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-9776584

RESUMEN

Accuracy of antigen determination in human plasma samples is often adversely affected by immune complex formation between antigens (e.g., HIV-1 p24 protein) and specific antibodies. In this study we describe an optimized method for complete immune complex dissociation (ICD) in plasma. This method is based on heat denaturation of antibodies and utilizes a defined solution of sodium dodecyl sulfate (SDS) and diethylenetriaminepentaacetic acid (DTPA) as diluent. The efficiency of this procedure for ICD was compared with those of published methods, employing heat denaturation alone and acidification. Plasma samples from patients participating in anti-retroviral treatments and samples reconstituted in vitro were treated and analyzed in parallel. HIV-1 p24 antigen was determined by quantitative enzyme-linked immunosorbent assay (ELISA). In 312 samples from 97 patients, antigenemia was found in 44.9% when measured directly and in 87.2% after this treatment. In a subset of 56 samples, 21.4% tested positive prior to treatment, while after either novel treatment, heat denaturation or acidification, these samples tested positive in 80.4%, 62.5% and 60.7%, respectively. In 94% of cases viral RNA was detected. This improved procedure for ICD provides a reliable and convenient method for complete and accurate p24 antigen detection in human plasma and is applicable to commercially available test kits.


Asunto(s)
Complejo Antígeno-Anticuerpo/química , Proteína p24 del Núcleo del VIH/aislamiento & purificación , Infecciones por VIH/virología , VIH-1/aislamiento & purificación , Carga Viral , Viremia/virología , Complejo Antígeno-Anticuerpo/sangre , Ensayo de Inmunoadsorción Enzimática , Anticuerpos Anti-VIH/inmunología , Proteína p24 del Núcleo del VIH/sangre , Proteína p24 del Núcleo del VIH/inmunología , Infecciones por VIH/sangre , VIH-1/genética , VIH-1/inmunología , Calor , Humanos , Ácido Pentético , Plasma , Desnaturalización Proteica , ARN Viral/sangre , Juego de Reactivos para Diagnóstico , Reproducibilidad de los Resultados , Dodecil Sulfato de Sodio
15.
J Immunol Methods ; 106(2): 257-65, 1988 Feb 10.
Artículo en Inglés | MEDLINE | ID: mdl-2828478

RESUMEN

This paper describes the construction of a new heteromyeloma cell line designated CB-F7. The cell line was derived from xenogeneic somatic cell hybridization between normal human B lymphocytes and the murine HAT-sensitive P3X63Ag8/653 cell line. CB-F7 cells were characterized by rapid cell growth (doubling time about 16 h) and high cloning efficiencies in culture medium supplemented with 10% or 5% fetal calf serum, respectively. The karyotype of the cells consists of about 75-78 chromosomes as well as two chromosomal fragments. Fusions of the cells with human peripheral blood cells resulted in approximately 2-6 clones per 10(5) seeded lymphocytes. Furthermore, the cells are ouabain resistant and therefore suitable for fusions with EBV-transformed lymphoblastoid cell lines. Using CB-F7 as the parental cell line a number of specific human mAb producing hybrids were established. For the first time, we describe here the generation of hybrids secreting human monoclonal antibodies to human immunodeficiency virus (HIV). Two monoclonal antibodies of IgG type and one of IgM type reacted with the major core protein p25 and one IgG antibody reacted with the transmembrane protein gp41.


Asunto(s)
Anticuerpos Monoclonales/inmunología , Anticuerpos Antivirales/inmunología , Linfocitos B/inmunología , Línea Celular , VIH/inmunología , Células Híbridas/citología , Hibridomas/citología , Fusión Celular , Transformación Celular Viral , Herpesvirus Humano 4 , Humanos , Inmunoglobulina G/inmunología , Inmunoglobulina M/inmunología , Cariotipificación , Factores de Tiempo
16.
Biotechniques ; 22(4): 730-5, 1997 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-9105625

RESUMEN

Based on the method of direct cloning into the baculovirus genome by linearizing and re-ligation in presence of the target insert, we designed viral constructs that express foreign genes on the surface of baculovirus particles. We chose the glycosylated envelope protein gp41 of human immunodeficiency virus type 1 (HIV-1) as a model for displaying recombinant proteins on budded virus. The ectodomain of the envelope protein gp41 of HIV-1 was being fused to the entire baculovirus major coat protein gp64 (Ac-cops41) and to the membrane anchor sequence of gp64 (Acmars41). Two different promoters, the "very late" polyhedrin promoter (Ac-mars41) and the "early and late" gp64 promoter (Ac-promars41) were compared. The expression of gp41 in infected cells and its presence on viral particles was confirmed by enzyme-linked immunosorbent assay (ELISA), Western blot and electron microscopy.


Asunto(s)
Baculoviridae/genética , Expresión Génica , Proteína gp41 de Envoltorio del VIH/genética , Proteínas Recombinantes de Fusión/genética , Proteínas Virales de Fusión/genética , Animales , Western Blotting , Ensayo de Inmunoadsorción Enzimática , Vectores Genéticos , Proteína gp41 de Envoltorio del VIH/biosíntesis , Humanos , Microscopía Electrónica , Proteínas Recombinantes de Fusión/biosíntesis , Spodoptera/genética
17.
Biotechniques ; 16(1): 140-7, 1994 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-8136128

RESUMEN

A completely automated pilot plant used for fermentation has been employed with direct digital control (DDC) technology for monitoring and regulating growth of human cells. A human hybridoma cell line (3D6) producing anti-human immunodeficiency virus (HIV)-1 antibodies was used as a model for large-scale production (300-liter airlift fermentor) in continuous culture. Parameters controlled were pH, dissolved oxygen, temperature and the flow rate of four gases used in the process. A control strategy was implemented to achieve constant fluid velocity and mixing by maintaining the rate of gas flow at a constant level. Another advantage of this approach was that the total gas flow required for optimal fluid circulation was reduced from 1 volume gas/volume fermenter/hour (vvh) to 0.3 vvh. Use of a low flow rate eliminated the serious problems of foaming, which contributed significantly to cell destruction, shorter filter-life and other considerations. Dilution rate was optimized at laboratory scale for maximum productivity, which results in relatively low viability. At a dilution rate of 0.0076 h-1, a total cell density of 6-7 x 10(5) cells/ml with a viability of approximately 75% was maintained during long-term continuous cultivation. These growth conditions resulted in a product titer stabilized in the range of 35 micrograms IgG/ml. Batchwise purification was achieved with a recovery of more than 50% and a final purification of active monoclonal antibody representing about 99% product. Results from isoelectric focusing and Western blotting demonstrated batch-to-batch consistency of the purified human monoclonal antibody to HIV-1 during the continuous growth process.(ABSTRACT TRUNCATED AT 250 WORDS)


Asunto(s)
Anticuerpos Monoclonales/biosíntesis , Anticuerpos Anti-VIH/biosíntesis , VIH-1/inmunología , Anticuerpos Monoclonales/aislamiento & purificación , Línea Celular , Computadores , Anticuerpos Anti-VIH/aislamiento & purificación , Humanos
18.
Immunol Lett ; 37(1): 41-5, 1993 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-8225405

RESUMEN

Based on our findings that HIV-1 gp41 independently of CD4 can bind to the helper T cell line H9 and B cell line Raji, we characterized the putative binding of HIV-1 gp41 to the monocyte cell lines U937 and HL60. Using flow cytometry (FACS) we examined the binding of soluble gp41 (sgp41; env amino acids 539-684) to these monocyte cell lines. Using sgp41 attached to Sepharose beads, U937 cell lysates were absorbed. The sgp41 eluate of U937 cell lysates could inhibit sgp41 binding to U937 cells. With SDS-PAGE of sgp41 eluate of U937 cell lysates, three strong protein bands, (37, 45 and 62 kDa) and two weak bands (49 and 92 kDa) were stained with Coomassie blue. With Western blot (ligand blot) analysis using sgp41, three strong protein bands (37, 49 and 62 kDa) and a very weak band (42-45 kDa) were observed in sgp41 eluate of U937 cell lysates. The results suggest that the four proteins 37, 42-45, 49 and 62 kDa in U937 cell lysates are possible candidates for the putative gp41 receptor(s). We compared the blocking activities of sgp41 eluates from different cell lysates. Not only U937 and Raji lysate-sgp41 eluates, but also H9 and HL60 lysate-sgp41 eluates could block sgp41 binding to U937 and Raji cells. The results indicate that the sgp41-binding proteins on U937, or Raji (H9 and HL60, respectively) probably could have an identical blocking (or binding) specificity; these cell types carry very similar receptor(s) for HIV-1 gp41 binding.


Asunto(s)
Proteína gp41 de Envoltorio del VIH/metabolismo , VIH-1/metabolismo , Monocitos/metabolismo , Proteínas del Envoltorio Viral/metabolismo , Linfocitos B/metabolismo , Unión Competitiva , Western Blotting , Electroforesis en Gel de Poliacrilamida , Citometría de Flujo , Anticuerpos Anti-VIH/metabolismo , Humanos , Peso Molecular , Receptores del VIH/metabolismo , Células Tumorales Cultivadas
19.
Immunol Lett ; 39(3): 219-22, 1994 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-7518416

RESUMEN

Several examples of molecular mimicry between HIV-1 and human proteins are reported in the literature. Here we report on yet another example. The monoclonal antibody 3D6 recognized a 17-amino-acid region in HIV-1 gp41 (amino acids 602-618) and could bind to the human T-cell lines H9 and Molt4, B cell lines Raji and Bjab, and monocyte cell lines U937 and HL60. By Western blot using 3D6, a strong band of 43 kDa and a very weak band of 80 kDa were detected in lysates of H9, Molt4, Raji and Bjab cell lines, but only a strong band of 43 kDa was observed in case of U937 and HL60 cells, under reducing or non-reducing conditions. The results indicate the presence of a common immunologic determinant between HIV-1 gp41 and membrane proteins of these human T, B and monocyte cells.


Asunto(s)
Linfocitos B/inmunología , Proteína gp41 de Envoltorio del VIH/inmunología , VIH-1/inmunología , Leucocitos/inmunología , Monocitos/inmunología , Linfocitos T/inmunología , Secuencia de Aminoácidos , Anticuerpos Monoclonales , Western Blotting , Línea Celular , Epítopos/inmunología , Citometría de Flujo , Humanos , Datos de Secuencia Molecular
20.
AIDS Res Hum Retroviruses ; 14(13): 1115-28, 1998 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-9737583

RESUMEN

We have stabilized a panel of 33 hybridomas producing human monoclonal antibodies (MAbs) against HIV-1 gp160 and p24. Five of these antibodies were able to neutralize different HIV-1 isolates, and two of them (2F5 and 2G12) revealed remarkable potential to neutralize primary virus isolates of different clades in several in vitro tests. To determine whether a structural basis for neutralization could be identified, we analyzed the antibodies at the molecular level. This study reports the primary nucleotide and deduced amino acid sequences of the rearranged heavy and light chain V segments (VH, Vkappa) of the neutralizing MAbs (1B1, 1F7, 2F5, 2G12, and 3D5) and the nonneutralizing anti-gp41 MAb 3D6. Aligning the V segments with the nearest related germline genes illustrated the occurrence of somatic mutations. The neutralizing MAbs show mutational rates comparable to those of antibodies that appear in patients in whom the immune system is under constant antigenic pressure over a long period of time. In contrast, 3D6, which recognizes the immunodominant region on gp41, displays homologies as high as 97 and 98% compared with its VH and Vkappa germline genes. The diversity segments [D(H)] of 1B1, 1F7, 3D5, and 3D6 were assigned to single D(H) segments on the chromosomal D(H) locus. 2F5 presents a D(H) segment 52 nucleotides in length, which could be explained by fusion of two segments on the immunoglobulin heavy chain locus that have not yet been described as rearranged regions. 2G12 D(H) shows best homologies to a D(H) segment between D3-22 and D4-23. This D(H) segment could be the reason for the rare occurrence of antibodies competing with 2G12. Since this nearest related chromosomal region on the D(H) locus does not display recombination signals at the flanking regions, this segment is normally not taken into consideration as a site for immunoglobulin heavy chain rearrangement.


Asunto(s)
Anticuerpos Monoclonales/genética , Anticuerpos Anti-VIH/genética , VIH-1 , Secuencia de Aminoácidos , Secuencia de Bases , ADN Complementario/análisis , Proteína p24 del Núcleo del VIH/inmunología , Proteínas gp160 de Envoltorio del VIH/inmunología , VIH-1/inmunología , Humanos , Hibridomas , Cadenas Pesadas de Inmunoglobulina/genética , Cadenas Ligeras de Inmunoglobulina/genética , Datos de Secuencia Molecular , Pruebas de Neutralización , Reacción en Cadena de la Polimerasa/métodos , ARN/aislamiento & purificación
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