Ultrafast, low-power, PCB manufacturable, continuous-flow microdevice for DNA amplification.

The design and fabrication of a continuous-flow µPCR device with very short amplification time and low power consumption are presented. Commercially available, 4-layer printed circuit board (PCB) substrates are employed, with in-house designed yet industrially manufactured embedded Cu micro-resistive heaters lying at very close distance from the microfluidic network, where DNA amplification takes place. The 1.9-m-long microchannel in combination with desirably high flow velocities (for fast amplification) challenged the robustness of the sealing that was overcome with the development of a novel bonding method rendering the microdevice robust even at extreme pressure drops (12 bars). The proposed fabrication methods are PCB compatible, allowing for mass and reliable production of the µPCR device in the established PCB industry. The µPCR chip was successfully validated during the amplification of two different DNA fragments (and with different target DNA copies) corresponding to the exon 20 of the BRCA1 gene, and to the plasmid pBR322, a commonly used cloning vector in E. coli. Successful DNA amplification was demonstrated at total reaction times down to 2 min, with a power consumption of 2.7 W, rendering the presented µPCR one of the fastest and lowest power-consuming devices, suitable for implementation in low-resource settings. Detailed numerical calculations of the DNA residence time distributions, within an acceptable temperature range for denaturation, annealing, and extension, performed for the first time in the literature, provide useful information regarding the actual on-chip PCR protocol and justify the maximum volumetric flow rate for successful DNA amplification. The calculations indicate that the shortest amplification time is achieved when the device is operated at its enzyme kinetic limit (i.e., extension rate). Graphical abstract.

Proposal for the Implementation of Quality Standards in a Medical Unit Through Integration to the Hospital Information System.

Quality standards (QS) support and enhance health care services provided to patients and citizens, especially in sophisticated medical departments, such as Intensive Care Units (ICU). However, ICU staff lag behind in the adoption and compliance of QS protocols. In this paper, QS protocols implemented in the Intensive Care Unit of the Attiko University Hospital, a tertiary teaching hospital of the University of Athens, will be discussed. In this hospital, standardized procedures are implemented through the HIS, facilitating routine administration and services. We are aiming to facilitate educational processes and enhance staff compliance with the protocols by utilizing the Hospital Information System (HIS). In doing this, we propose the application of pop-up windows on the different user (medical or nursing) interfaces of the HIS, inter-connecting every electronic process with the corresponding QS protocol that has been developed in the ICU. This application may prove a valuable educational tool and may reinforce staff training and enhance compliance with the QS protocols.