Prolongation of electrode lifetime in biofuel cells by periodic enzyme renewal.

Enzymatically catalyzed biofuel cells show unique specificity and promise high power densities, but suffer from a limited lifetime due to enzyme deactivation. In the present work, we demonstrate a novel concept to extend the lifetime of a laccase-catalyzed oxygen reduction cathode in which we decouple the electrode lifetime from the limited enzyme lifetime by a regular resupply of fresh enzymes. Thereto, the adsorption behavior of laccase from Trametes versicolor to buckypaper electrode material, as well as its time-dependent deactivation characteristics, has been investigated. Laccase shows a Langmuir-type adsorption to the carbon nanotube-based buckypaper electrodes, with a mean residence time of 2 days per molecule. In a citrate buffer of pH 5, laccase does not show any deactivation at room temperature for 2 days and exhibits a half-life of 9 days. In a long-term experiment, the laccase electrodes were operated at a constant galvanostatic load. The laccase-containing catholyte was periodically exchanged against a freshly prepared one every second day to provide sufficient active enzymes in the catholyte for the replacement of desorbed inactive enzymes. Compared to a corresponding control experiment without catholyte exchange, this procedure resulted in a 2.5 times longer cathode lifetime of 19 ± 9 days in which the electrode showed a potential above 0.744 V vs. normal hydrogen electrode at 110 µA cm(-2). This clearly indicates the successful exchange of molecules by desorption and re-adsorption and is a first step toward the realization of a self-regenerating enzymatic biofuel cell in which enzyme-producing microorganisms are integrated into the electrode to continuously resupply fresh enzymes.

Photo-onycholysis from minocycline. Side effects of minocycline therapy.

Minocycline, prescribed for a patient with acne, produced a photosensitivity reaction (photo-onycholysis) involving the fingernails. The onset of this reaction coincided with an increase in sun exposure and cleared several months later, when the drug was discontinued. This type of reaction has been reported to occur in patients taking tetracycline or a member of the tetracycline group of antibiotics, of which minocycline is a member. This appears to be first report of photo-onycholysis due to minocycline.

[Technical reference: the oblique topogram as an adjustment aid]. / Technischer Hinweis: des schräge Topogramm als Einstellhilfe.

The article points to the possibility of producing a topogram with the gantry in oblique position. Although this yields a distorted image, it enables accurate planning of the section and also a localization of objects in slanted position, for example, the intervertebral disks of the lower lumbar vertebral column, thus saving the patient time, material and radiation exposure.

Assessment of coronary artery bypass grafts: value of different breath-hold MR imaging techniques.

OBJECTIVE: Our aim was to evaluate the patency of coronary artery bypass grafts and to detect graft stenosis using different breath-hold MR imaging techniques. SUBJECTS AND METHODS: Twenty-two patients with 59 grafts (14 internal mammary artery grafts and 45 saphenous vein grafts) and 76 distal anastomoses (singular and sequential grafts) were studied using a 1.5-T scanner. A two-dimensional T2-weighted breath-hold half-Fourier acquisition single-shot turbo spin echo (HASTE) sequence and a three-dimensional breath-hold contrast-enhanced MR angiography sequence (fast imaging with steady-state free precession) were performed. For MR angiography, a bolus of 20 ml of gadopentetate dimeglumine was used. Time delay for contrast injection was calculated by a test bolus. The gold standard was coronary angiography performed within 14 days of MR imaging. All images were evaluated independently by two radiologists. RESULTS: With the HASTE sequence, 95% of the patent grafts were recognized (42/44); specificity was 93% (14/15). MR angiography had both a sensitivity (41/44) and specificity (14/15) of 93%. Interobserver agreement for both sequences was good (Cohen's kappa = 87%; McNemar test, p = 56%). Forty-nine (83%) of 59 patent distal graft anastomoses were revealed with the HASTE sequence; 38 (64%) of 59 were seen on contrast-enhanced angiography. With HASTE imaging, only two of eight hemodynamically significant graft stenoses were detected. MR angiography revealed only four of eight significant graft stenoses. CONCLUSION: The HASTE sequence and three-dimensional MR angiography proved to be useful MR techniques when evaluating the patency of coronary artery bypass grafts. However, reliable detection of graft stenosis does not yet seem possible with these imaging techniques.