[Experimental base for internal connection dental implants for two-step implantation].

The article contains selected results of comprehensive studies on osseointegration of small diameter internal connection dental implants made of nanostructured titanium. Fatigue tests were carried out by means of new osseointegrated metal etching method.

[Chromogenic in situ hybridization in diagnostics of Epstein-Barr-virus infection in chronic tonsillitis].

In the issue we demonstrate results of Epstein-Barr virus (EBV) detection by chromogenic in situ hybridization (CISH) in epithelial and lymphoid cells of the palatine tonsil in patients with chronic tonsillitis. Virus genome detections were performed using RNA-probes with digoxigenin-labeled oligonucleotides which target EBV RNA, notably RNA-transcripts of virus genomic DNA. The obtained data confirm the virus lymphotropism and also tropism to epithelial cells of both surface and cryptal epithelium of the palatine tonsil. CISH method in combination with immunohistochemical identification of virus protein products opens new possibilities for clinicopathological monitoring of the different clinical forms of the chronic tonsillitis, as well as new horizon for understanding intrinsic role of EBV in tonsillar pathology.

[The application of immunoglobulins for intravenous administration for the combined treatment of paratonsillar abscesses].

The objective of the present study was to evaluate the possibility of using immunoglobilins for intravenous administration (IVIG) to treat the patients presenting with paratonsillar abscesses (PTA). The comparative assessment of the clinical and immunological status of 77 patients with PTA depending on the type of therapy (traditional or combined with the use of IVIG) and the occurrence of tonsillitis in the medical history (the newly diagnosed disease, frequent tonsillitis, relapses of PTA). The level s of immunoglobulins (sIgA, IgA, IgG and its subclasses, IgM, and IgE) in mixed saliva samples were determined by the immunoenzyme assay and the albumin level by the bromcresol method with the counting of relative secretion coefficients (RSC). Palatine tonsil (PT) biopsies were used to determine the absolute content of lymphocytes (Lf) that were classified into differentiation clusters (CD3, CD4, CD8, CD16, CD25, CD71, CD95, HLA-DR). The patients presenting with paratonsillar abscesses were characterized by impaired immune responsiveness of palatine tonsils manifest as reduced production of IgG (at the expense of IgG2). This reduction was especially pronounced in the cases with the burdened medical history (recurring tonsillitis and relapses of PTA). In such patients, traditional therapy resulted in suppression of the cellular component of immunity and IgG production. The treatment with IVIG increased the production of IgG antibodies in the patients with newly diagnosed tonsillitis; moreover it stimulates production of IgG and activated the T-cell component. At the same time, the administration of IVIG reduced the duration of hospital stay from 9.29±0.31 to 7.06±25 days and the number of PTA relapses. It is concluded that the low IgG content in the saliva may be an indication for tonsillectomy.

[Immunologic correctors in combined treatment of paratonsillitis]. / Immunokorrektory v kompleksnom lechenii paratonzillita.

As shown by immunological examination of 47 healthy subjects living in polluted territories and 120 patients with paratonsillitis (PT), PT arises in T-cell and humoral immunodeficiency of the palatine tonsils. Conventional treatment fails to eliminate initial immunodeficiency, contributes to development of chronic inflammation and complications. Adjuvant treatment with local polyvalent pyobacteriophage and parenteral placental albumin improves local and systemic immunity, promotes a favourable outcome of acute inflammation in the tonsils.

[Validation of therapeutical policy choice in exudative otitis media]. / Obosnovanie vybora taktiki lecheniia ékssudativnogo srednego ukha.

132 patients with diagnosis acute or subacute exudative otitis media were treated conservatively (n = 79) or surgically (n = 53). Conservative treatment was effective in 41% of the cases, surgical treatment produced positive results in 62.3%. The operative treatment combined elements of endoscopic nasal surgery and methods used in tympanoplasty, plastic treatment of the auditory tube, surgery of the internal ear.