RESUMEN
The function of nicotinamide adenine dinucleotide (NAD) and adenosine diphosphate (ADP) ribosylation reactions in the mechanism of apoptotic cell death is controversial, although one theory postulates an essential role for NAD depletion by poly-ADP-ribose polymerase. The present study examined the role of intracellular NAD in tumor necrosis factor (TNF) and ultraviolet (UV) light-induced activation of the 24-kD apoptotic protease (AP24) leading to internucleosomal DNA fragmentation and death. Our results demonstrate that nutritional depletion of NAD to undetectable levels in two leukemia lines (U937 and HL-60) renders them completely resistant to apoptosis. This was attributed to a block in the activation of AP24 and subsequent DNA cleavage. Normal cells show an elevation of ADP-ribosyl transferase (ADPRT) in both the cytosol and nucleus after exposure to TNF, but before DNA fragmentation. ADPRT activity as well as cell death was suppressed by an inhibitor specific for mono-ADPRT. Nuclei from NAD-depleted cells were still sensitive to DNA fragmentation induced by exogenous AP24, indicating a selective function for NAD upstream of AP24 activation in the apoptotic pathway. We confirmed a requirement for intracellular NAD, activation of ADPRT, and subsequent NAD depletion during apoptosis in KG1a, YAC-1, and BW1547 leukemia cell lines. However, this mechanism is not universal, since BJAB and Jurkat leukemia cells underwent apoptosis normally, even in the absence of detectable intracellular NAD. We conclude that TNF or UV light-induced apoptotic cell death is not due to NAD depletion in some leukemia cell lines. Rather, NAD-dependent reactions which may involve mono-ADPRT, function in signal transduction leading to activation of AP24, with subsequent DNA fragmentation and cell death.
Asunto(s)
Apoptosis/fisiología , NAD/deficiencia , Poli(ADP-Ribosa) Polimerasas/metabolismo , Serina Endopeptidasas/metabolismo , Transducción de Señal , 3-Yodobencilguanidina , Animales , Apoptosis/efectos de la radiación , Núcleo Celular/enzimología , Citosol/enzimología , Daño del ADN , Relación Dosis-Respuesta a Droga , Activación Enzimática , Humanos , Yodobencenos/farmacología , Ratones , Inhibidores de Poli(ADP-Ribosa) Polimerasas , Inhibidores de Serina Proteinasa/farmacología , Células Tumorales Cultivadas , Factor de Necrosis Tumoral alfa/farmacología , Rayos UltravioletaRESUMEN
We report the purification of a protease from tumor cells undergoing apoptosis that is involved in activating DNA fragmentation. Initial studies revealed that two inhibitors of serine proteases, N-1-tosylamide-2-phenylethylchloromethyl ketone and carbobenzoxy-Ala-Ala-borophe (DK120), suppressed tumor necrosis factor or ultraviolet (UV) light-induced DNA fragmentation in the U937 histiocytic lymphoma as well as UV light-induced DNA fragmentation in the BT-20 breast carcinoma, HL-60 myelocytic leukemia, and 3T3 fibroblasts. The protease was purified by affinity chromatography with DK120 as ligand and showed high activity on a synthetic substrate preferred by elastase-like enzymes (Ala-Ala-Pro-Val p-nitroanilide), but was inactive on the trypsin substrate, N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester, or the chymotrypsin substrate, Ala-Ala-Pro-Phe p-nitroanilide. The activity of the DK120-binding protease purified from U937 cells undergoing apoptosis was increased approximately 10-fold over that recovered from normal cells. Further purification to homogeneity by heparin-Sepharose affinity chromatography followed by reverse phase high-performance liquid chromatography revealed a single band of 24 kD on a silver-stained sodium dodecyl sulfate gel. In addition to protease activity, the purified enzyme induced DNA fragmentation into multiples of 180 basepairs in isolated U937 nuclei. These findings suggest the 24-kD protease is a novel enzyme that activates DNA fragmentation in U937 cells undergoing apoptosis.
Asunto(s)
Apoptosis , Daño del ADN , ADN de Neoplasias/metabolismo , Desoxirribonucleasas/metabolismo , Endopeptidasas/metabolismo , Células 3T3 , Secuencia de Aminoácidos , Animales , Neoplasias de la Mama , Línea Celular , Cromatografía de Afinidad , Desoxirribonucleasas/aislamiento & purificación , Endopeptidasas/aislamiento & purificación , Humanos , Cinética , Leucemia Promielocítica Aguda , Linfoma de Células B Grandes Difuso , Ratones , Datos de Secuencia Molecular , Peso Molecular , Inhibidores de Proteasas/farmacología , Especificidad por Sustrato , Células Tumorales Cultivadas , Factor de Necrosis Tumoral alfa/farmacología , Rayos UltravioletaRESUMEN
The 24-kD apoptotic protease (AP24) is a serine protease that is activated during apoptosis and has the capacity to activate internucleosomal DNA fragmentation in isolated nuclei. This study examined the following: (a) the functional relationship between AP24 and the CPP32-like proteases of the caspase family; and (b) whether activation of CPP32-like proteases is sufficient to commit irreversibly a cell to apoptotic death. In three different leukemia cell lines, we showed that agents that directly (carbobenzoxy-Ala-Ala-borophe (DK120) or indirectly inhibit activation of AP24 (protein kinase inhibitors, basic fibroblast growth factor, tosylphenylalaninechloromethylketone, and caspase inhibitors) protected cells from apoptosis induced by TNF or UV light. Only the caspase inhibitors, however, prevented activation of CPP32-like activity as revealed by cleavage of the synthetic substrate, DEVD-pNa, by cell cytosols, and also by in vivo cleavage of poly (ADP-ribosyl) polymerase, a known substrate of CPP32. Activation of DEVD-pNa cleaving activity without apoptosis was also demonstrated in two variants derived from the U937 monocytic leukemia in the absence of exogenous inhibitors. Cell-permeable peptide inhibitors selective for CPP32-like proteases suppressed AP24 activation and apoptotic death. These findings indicate that CPP32-like activity is one of several upstream signals required for AP24 activation. Furthermore, activation of CPP32-like proteases alone is not sufficient to commit irreversibly a cell to apoptotic death under conditions where activation of AP24 is inhibited.
Asunto(s)
Apoptosis , Caspasas , Cisteína Endopeptidasas/metabolismo , Serina Endopeptidasas/metabolismo , Apoptosis/efectos de los fármacos , Caspasa 1 , Caspasa 3 , Permeabilidad de la Membrana Celular , Inhibidores de Cisteína Proteinasa/farmacología , Fragmentación del ADN , Activación Enzimática , Factor 2 de Crecimiento de Fibroblastos/farmacología , Humanos , Linfoma , Oligopéptidos/farmacología , Poli(ADP-Ribosa) Polimerasas/metabolismo , Inhibidores de Proteínas Quinasas , Inhibidores de Serina Proteinasa/farmacología , Transducción de Señal/fisiología , Clorometilcetona de Tosilfenilalanila/farmacología , Células Tumorales Cultivadas , Factor de Necrosis Tumoral alfa/farmacología , Rayos UltravioletaRESUMEN
Studies on the mechanism of apoptosis in this laboratory support a model in which signal transduction involving caspase 3 leads to activation of a serine protease called Mr 24,000 apoptotic protease (AP24), which then induces internucleosomal DNA fragmentation in the nucleus. This study examined the effect of Bcl-2 overexpression on activation of AP24 and the induction of DNA fragmentation by AP24 in isolated nuclei. It was demonstrated that overexpression of Bcl-2 in either HL-60 or PW leukemia cell lines suppressed activation of AP24 induced by either tumor necrosis factor or UV light and protected cells from apoptosis. Furthermore, nuclei isolated from Bcl-2-overexpressing cells were relatively resistant to internucleosomal DNA fragmentation induced by AP24 isolated from apoptotic cells. Bcl-2-overexpressing cells that were nutritionally depleted of glutathione (GSH) became sensitive to tumor necrosis factor- or UV light-induced activation of AP24 and underwent apoptotic cell death. Moreover, nuclei isolated from Bcl-2-overexpressing cells that were depleted of GSH became sensitive to AP24-induced DNA fragmentation. The addition of exogenous GSH blocked the proteolytic activity of AP24, as well as its ability to induce DNA fragmentation in normal isolated nuclei. These results indicate that Bcl-2 can attenuate at least two events in the AP24 apoptotic pathway: activation of AP24 and induction of DNA fragmentation by activated AP24. Furthermore, agents that deplete intracellular levels of GSH may have therapeutic use in the sensitization of Bcl-2-overexpressing cancer cells to apoptotic cell death.
Asunto(s)
Apoptosis/fisiología , ADN de Neoplasias/metabolismo , Glutatión/farmacología , Proteínas Proto-Oncogénicas c-bcl-2/fisiología , Serina Endopeptidasas/farmacología , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Núcleo Celular/efectos de la radiación , Daño del ADN , ADN de Neoplasias/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Glutatión/metabolismo , Glutatión/fisiología , Células HL-60/efectos de los fármacos , Células HL-60/metabolismo , Células HL-60/efectos de la radiación , Humanos , Nucleosomas/efectos de los fármacos , Nucleosomas/metabolismo , Nucleosomas/efectos de la radiación , Oligopéptidos/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/biosíntesis , Serina Endopeptidasas/fisiología , Factor de Necrosis Tumoral alfa/farmacología , Rayos UltravioletaRESUMEN
A series of 1-acylamino boronic acids (IA-VA), analogues of the amino acids phenylalanine, phenylglycine, alanine, valine, and isoleucine, were prepared as potential transition-state inhibitors of the serine proteases alpha-chymotrypsin and elastase, by a boronate homologation reaction. The corresponding difluoroboranes (IB-VB), produced from the boronic acids by treatment with HF, were more easily purified than the boronic acids. Since the difluoroboranes readily hydrolyze in water, they proved to be convenient precursors for the boronic acids. The phenylalanine and phenylglycine analogues I and II were good competitive inhibitors of alpha-chymotrypsin (Ki = 0.3-8 microM), and the alanine, valine, and isoleucine analogues (III-IV) proved to be good inhibitors of elastase (Ki = 0.1-35 microM). On the basis of their high affinity and the tendency of boronic acids to form borate complexes, these acylamino boronic acids may be behaving as transition-state inhibitors.
Asunto(s)
Aminoácidos , Boranos/farmacología , Ácidos Borónicos/farmacología , Quimotripsina/antagonistas & inhibidores , Elastasa Pancreática/antagonistas & inhibidores , Acilación , Alanina/análogos & derivados , Unión Competitiva , Boranos/síntesis química , Ácidos Borónicos/síntesis química , Fenómenos Químicos , Química , Glicina/análogos & derivados , Hidrólisis , Isoleucina/análogos & derivados , Fenilalanina/análogos & derivados , Relación Estructura-Actividad , Valina/análogos & derivadosRESUMEN
Dihydroorotase (DHO) catalyzes the conversion of carbamyl aspartate (CA) to dihydroorotate (DO) in the de novo pyrimidine biosynthetic pathway. Few effective inhibitors of DHO have been reported, and thus blockade of this reaction has not been widely pursued as a strategy for development of antitumor agents. Utilizing two mechanism-based strategies, we have designed and prepared potential DHO inhibitor analogues of CA. One strategy replaced the gamma-carboxyl moiety of CA with a boronic acid. This substitution yields compounds which form stable charged tetrahedral intermediates and mimic the enzyme-substrate transition state. Preparation of the boronic acid analogues of CA and its carboxylic acid esters focused on a Curtius rearrangement as a key step following a malonic ester synthesis. This was followed by carbamoylation of the free amine under nonaqueous neutral conditions with Si(NCO)4. The ethyl ester was a competitive inhibitor of DHO with an apparent Ki of 5.07 microM, while the nonesterified analogue and the methyl ester were not effective inhibitors. None of the compounds were cytotoxic against L1210 cells in culture. An active-site-directed sulfhydryl-containing zinc chelator was also prepared. This analogue irreversibly inhibited the enzyme, but it also was ineffective in L1210 growth inhibition.
Asunto(s)
Amidohidrolasas/antagonistas & inhibidores , Ácidos Borónicos , Dihidroorotasa/antagonistas & inhibidores , Inhibidores Enzimáticos/síntesis química , Homocisteína , Animales , División Celular/efectos de los fármacos , Quelantes , Diseño de Fármacos , Leucemia L1210 , Células Tumorales Cultivadas/efectos de los fármacos , ZincRESUMEN
A series of chemically reactive derivatives of the nonsteroidal estrogen hexestrol have been synthesized as potential affinity labels for the estrogen receptor or as cytotoxic agents with selective activity against receptor-containing cells. These compounds are hexestrol ethers with halo ketone, halohydrin, or epoxide functions or 4-substituted deoxyhexestrols with halo ketone, benzyl halide, nitro, azide, sulfonyl fluoride, or sulfonyl azide groups. The alkylating activity of the electrophilic derivatives was measured using the colorimetric reagent nitrobenzylpyridine, the bromo derivatives being considerably more reactive than the chloro ones. Their reversible binding to the lamb uterine estrogen receptor was measured by competitive binding assays, and their irreversible reaction with receptor was measured by exchange assays that determine the rate and extent of receptor inactivation. In general, monoetherification of hexestrol or substitution of deoxyhexestrol produces compounds with relatively low affinity for the estrogen receptor (0.3-10% that of estradiol). Most of the electrophilic derivatives are rapid and effective inactivators of receptor (24-70% inactivation within 0.5-5 h at 25 degrees C). Of the photosensitive derivatives, 4-azidodeoxyhexestrol appears to be the most efficient receptor inactivator (49%). The high reactivity of these compounds toward the estrogen receptor and the lack of interference by their reaction with other cellular nucleophiles suggest that these compounds may be useful as affinity-labeling agents or as selective cytotoxic agents in intact systems.
Asunto(s)
Marcadores de Afinidad/farmacología , Alquilantes/farmacología , Estrógenos/farmacología , Receptores de Estrógenos/metabolismo , Alquilantes/síntesis química , Sitios de Unión , Estrógenos/síntesis química , Estrógenos/metabolismo , Hexestrol/síntesis química , Hexestrol/metabolismo , Fotólisis , Receptores de Estrógenos/efectos de los fármacos , Relación Estructura-ActividadRESUMEN
Three hydroxyethylamine analogues of angiotensins II, III, and IV were prepared by solid-phase methods. The resin-bound peptide was alkylated with the iodomethylketone derivative of the N-terminal amino acid, followed by reduction to the alcohol using sodium borohydride. The iodomethylketones can be made in good yields from commercially available N-protected amino acids. The compounds were evaluated for their ability to displace labeled angiotensins from bovine adrenal membranes, and their metabolic stability tested in kidney homogenates and aminopeptidase M preparations. The hydroxyethylamine amide bond replacement reduced the affinity of the analogues; however, they were substantially more stable to enzymatic degradation.
Asunto(s)
Aminoácidos/química , Angiotensina II/análogos & derivados , Hidrocarburos Halogenados/química , Cetonas/química , Glándulas Suprarrenales/efectos de los fármacos , Glándulas Suprarrenales/ultraestructura , Aminopeptidasas/metabolismo , Angiotensina II/síntesis química , Angiotensina II/farmacología , Animales , Automatización , Unión Competitiva , Bovinos , Riñón/efectos de los fármacos , Riñón/ultraestructura , Masculino , Membranas/efectos de los fármacos , Metionil Aminopeptidasas , Ratas , Ratas Sprague-Dawley , Relación Estructura-ActividadRESUMEN
The chemical breakdown of carmethizole [1-methyl-2-methylthio-4,5-bis-(hydroxymethyl)imidazole-4',5'- bis(N-methylcarbamate)hydrochloride] and its pharmacokinetics in the mouse and beagle dog were studied. Carmethizole was relatively unstable in aqueous media, having a half-life of less than or equal to 1 h in 0.9% sodium chloride, human whole blood, human plasma, and dog urine at 37 degrees C. Its major breakdown product in 0.9% sodium chloride and pH 5.0 sodium phosphate buffer was carmethizole diol. When carmethizole was added to pH 7.0 or pH 9.0 sodium phosphate buffer, the major breakdown product was carmethizole diol-4'-monophosphate. Carmethizole reacted directly with glutathione at pH 8.0, forming a glutathione adduct of carmethizole monocarbamate. Elimination of the drug from the plasma of the beagle dog following i.v. bolus doses of 22.4 and 4.3 mg/kg was biphasic. At these doses the terminal half-life was 39 and 46 min, respectively, and the respective total body clearance was 4.6 and 7.7 ml/min per kg. The 22.4 mg/kg dose was lethal to the beagle dog by day 4. Elimination of carmethizole from the plasma of mice following an i.v. bolus dose of 115 mg/kg was monoexponential, with a half-life of 11.6 min and a total body plasma clearance of 43.6 ml/min per kg. When the drug was infused at 230 mg/kg over 8 h into mice, the total body clearance was 40.8 ml/min per kg. Following the i.v. bolus administration of carmethizole to mice, 30% of the total dose was excreted in urine over 3 h as carmethizole diol, 10%, as carmethizole diol-sulfate, 3.4%, as carmethizole 4'-monocarbamate, and 2.4%, as unchanged drug.
Asunto(s)
Antineoplásicos/farmacología , Imidazoles/farmacología , Animales , Antineoplásicos/análisis , Antineoplásicos/farmacocinética , Cromatografía Líquida de Alta Presión , Perros , Interacciones Farmacológicas , Estabilidad de Medicamentos , Glutatión/metabolismo , Concentración de Iones de Hidrógeno , Imidazoles/análisis , Imidazoles/farmacocinética , Espectroscopía de Resonancia Magnética , Masculino , Espectrometría de Masas , Ratones , Ratones Endogámicos , Microsomas Hepáticos/efectos de los fármacos , Microsomas Hepáticos/metabolismo , Ratas , Ratas Endogámicas F344 , Soluciones , Factores de TiempoRESUMEN
We assayed urokinase plasminogen activator (uPA), tissue type plasminogen activator (tPA), and plasminogen activator inhibitor-1 (PAI-1) in 43 human brain tumors (predominantly astrocytic gliomas) and in histologically disease-free brain tissue resected with 21 of the tumors. Levels of uPA, tPA, and PAI-1, measured by enzyme-linked immunosorbent assay, varied widely among individuals in neoplastic and in normal tissue but did not correlate with age or sex. Pairwise comparison of neoplastic and normal tissue from 21 individuals revealed that mean tumor uPA level was elevated 6-fold (P < 0.001). Mean tumor tPA and PAI-1 were 2.5-fold greater than those of normal brain, but these differences were not statistically significant. Tumor uPA was elevated 2- to 30-fold in 16/21 paired samples (76%). In contrast, tumor tPA was elevated 2- to 22-fold in 7/21 (33%) of pairs, whereas tumor PAI-1 was 2- to 13-fold greater in 10/21 (48%) of pairs. Our results demonstrate that elevation of uPA content is frequent in astroglial tumors, as is the case in other major human cancers.
Asunto(s)
Astrocitoma/enzimología , Neoplasias Encefálicas/enzimología , Encéfalo/enzimología , Activador de Plasminógeno de Tipo Uroquinasa/metabolismo , Adolescente , Adulto , Anciano , Niño , Preescolar , Ensayo de Inmunoadsorción Enzimática , Femenino , Glioblastoma/enzimología , Humanos , Masculino , Persona de Mediana Edad , Inhibidor 1 de Activador Plasminogénico/metabolismo , Valores de Referencia , Activador de Tejido Plasminógeno/metabolismoRESUMEN
Brominated and chlorinated haloacetates (HAs) are by-products of drinking water disinfection. Dichloroacetate (DCA) and trichloroacetate (TCA) are hepatocarcinogenic in rodents, but the brominated analogs have received little study. Prior work has indicated that acute doses of the brominated derivatives are more potent inducers of oxidative stress and increase the 8-hydroxydeoxyguanosine (8-OH-dG) content of the nuclear DNA in the liver. Since, DCA and TCA are also known as weak peroxisome proliferators, the present study was intended to determine whether this activity might be exacerbated by peroxisomal proliferation. Classical responses to peroxisome proliferators, cyanide-insensitive acyl-CoA oxidase activity and increased 12-hydroxylation of lauric acid, were elevated in a dose-related manner in mice maintained on TCA and clofibric acid (positive control), but not with DCA, dibromoacetate (DBA) or bromochloroacetate (BCA). Administration of the HAs in drinking water to male B6C3F1 mice for periods from 3 to 10 weeks resulted in dose-related increases in 8-OH-dG in nuclear DNA of the liver with DBA and BCA, but not with TCA or DCA. These findings indicate that oxidative damage induced by the haloacetates is, at least in part, independent of peroxisome proliferation. In addition, these data suggest that oxidative damage to DNA may play a more important role in the chronic toxicology of brominated compared to the chlorinated haloacetates.
Asunto(s)
Acetatos/toxicidad , Sistema Enzimático del Citocromo P-450/metabolismo , Daño del ADN , Hígado/efectos de los fármacos , 8-Hidroxi-2'-Desoxicoguanosina , Acetatos/administración & dosificación , Acil-CoA Oxidasa , Animales , División Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Ácido Clofíbrico/metabolismo , Ácido Clofíbrico/toxicidad , Citocromo P-450 CYP4A , Desoxiguanosina/análogos & derivados , Desoxiguanosina/metabolismo , Ácido Dicloroacético/administración & dosificación , Ácido Dicloroacético/toxicidad , Ingestión de Líquidos , Agua Dulce , Hidroxilación , Ácidos Láuricos/metabolismo , Hígado/citología , Hígado/enzimología , Masculino , Ratones , Microcuerpos/efectos de los fármacos , Microcuerpos/metabolismo , Oxigenasas de Función Mixta/metabolismo , Estrés Oxidativo , Oxidorreductasas/metabolismo , Estándares de Referencia , Contaminantes Químicos del Agua/toxicidadRESUMEN
Di- and tripeptide boro-amino acid analog protease inhibitors with specificity for chymotrypsin and elastase decrease the number of melanotic foci formed in the lungs of mice in the B16BL6 experimental metastatic tumor model. These effects were at significantly lower concentration than leupeptin or other natural chymotrypsin inhibitors previously reported. These results support the involvement of elastase and chymotrypsin in the metastatic process.
Asunto(s)
Ácidos Borónicos/farmacología , Melanoma Experimental/tratamiento farmacológico , Inhibidores de Serina Proteinasa/farmacología , Animales , Ácidos Borónicos/síntesis química , División Celular/efectos de los fármacos , Quimotripsina/antagonistas & inhibidores , Melanoma Experimental/secundario , Ratones , Ratones Endogámicos , Metástasis de la Neoplasia/prevención & control , Elastasa Pancreática/antagonistas & inhibidores , Inhibidores de Serina Proteinasa/síntesis química , Células Tumorales CultivadasRESUMEN
Chlorinated, brominated, and mixed bromochloro acetates are major by-products of water disinfection by chlorine or ozone. The chlorinated acetates, trichloroacetate (TCA) and dichioroacetate (DCA), are carcinogenic in rodents. Brominated analogs of TCA and DCA have received little study. TCA and DCA induce lipid peroxidation in the livers of rodents when administered acutely. Oxidative stress can also result in oxidative damage to DNA, most commonly measured as increases in 8-hydroxydeoxyguanosine (8-OHdG) adducts. In this study, the ability of acute doses of TCA, DCA, dibromoacetate (DBA), bromodichloroacetate (BDCA), and bromochloroacetate (BCA) to induce lipid peroxidation and 8-OHdG formation was examined. Male B6C3F1 mice developed significant increases in 8-OHdG/dG ratios in nuclear DNA isolated from livers when treated with haloacetates. The extent of 8-OHdG formation appeared to be related to the ability to induce thiobarbituric acid-reactive substances (TBARS). The order of potency was DBA = BCA > BDCA > DCA > TCA. The induction of 8-OHdG was found to be generally more sensitive to treatment with haloacetates than the TBARS response. Significantly elevated levels of 8-OHdG were observed at doses of DBA, BCA, and BDCA as low as 30 mg/kg. We suggest that formation of 8-OHdG by brominated haloacetates may contribute to their toxicological effects.
Asunto(s)
Acetatos/toxicidad , Desoxiguanosina/análogos & derivados , Peroxidación de Lípido/efectos de los fármacos , 8-Hidroxi-2'-Desoxicoguanosina , Animales , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Cromatografía Líquida de Alta Presión , ADN/química , ADN/aislamiento & purificación , ADN/metabolismo , Aductos de ADN/química , Desoxiguanosina/metabolismo , Electroquímica , Halógenos , Hígado/química , Hígado/metabolismo , Masculino , Ratones , Ratones Endogámicos , Espectrofotometría Ultravioleta , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
AP24 is a serine protease that is activated during TNF or UV light-induced apoptosis and stimulates DNA fragmentation in isolated nuclei. The present study determined whether apoptosis induced by chemotherapeutic drugs resulted in activation of AP24 and examined the possible relationship to caspase activity. We showed that an inhibitor of AP24, DK120, could block DNA fragmentation induced in three leukemia cell lines (U937, HL-60, and CEM) by various DNA-damaging drugs including etoposide, camptothecin, chlorambucil, and the CC1065-related drug, YW201. Etoposide-induced activation of intracellular DEVD-pNa cleaving activity and apoptosis was suppressed by low micromolar concentrations of cell-permeable inhibitors of caspase-3. Furthermore, these inhibitors also suppressed activation of AP24. In contrast, DK120 did not prevent etoposide activation of DEVD-pNa cleaving activity, nor did it prevent cleavage of poly(ADP-ribose) polymerase. AP24 isolated from apoptotic cells following treatment with etoposide activated DNA fragmentation in isolated normal nuclei and was inhibited by DK120, but not by caspase inhibitors. This evidence shows that activation of caspase 3-like proteases generates signals that contribute to the activation of AP24 which may then induce nuclear DNA fragmentation in chemotherapeutic drug-induced apoptosis.