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5.
Lymphology ; 55(1): 1-9, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35896110

RESUMEN

Lymphoscintigraphy with single-photon emission computed tomography (SPECT-CT) is useful in diagnosing lymphedema. However, there are multiple timings, techniques, and tracers utilized worldwide without any comparison. We examined and compared the image clarity with two different radiotracers, 99mTc human serum albumin (HSA) and 99mTc phytate (phytate), in the same patients. The study retrospectivity examined 46 limbs of 36 patients who underwent lymphoscintigraphy using HSA and phytate from January 2013 to September 2018. Tracer accumulation in the lymph nodes, linear pattern (LP), and dermal backflow (DBF) were qualitatively analyzed; contrast-to-noise ratios (CNR) of DBF and standardized uptake value ratio (SUVR) of LP were also quantitatively analyzed. Neither lymph node accumulation nor DBF identification showed significant difference. However, a significant difference was observed between the LP identification of the unaffected (p<0.001) and affected sides (p<0.001). On quantitative evaluation, CNR and SUVR of LP was significantly higher with HSA than with phytate (p<0.001). SUVR of LP was also significantly higher with HSA than with phytate in both unaffected (p=0.002) and affected (p=0.005) sides. Overall, images acquired with HSA were clearer than that with phytate, and the identification of LP was particularly better with HSA than with phytate. Thus, lymphoscintigraphy using HSA is preferred over phytate for both diagnosis and evaluation of disease severity and surgical site selection.


Asunto(s)
Linfedema , Linfocintigrafia , Humanos , Extremidad Inferior , Linfedema/diagnóstico por imagen , Ácido Fítico , Agregado de Albúmina Marcado con Tecnecio Tc 99m , Tomografía Computarizada de Emisión de Fotón Único , Tomografía Computarizada por Rayos X
8.
J Org Chem ; 65(11): 3371-8, 2000 Jun 02.
Artículo en Inglés | MEDLINE | ID: mdl-10843619

RESUMEN

Irradiation of various meta- and para-substituted homobenzoquinones with ethyl vinyl ether gave the [2 + 2] photoadducts, tricyclic diones, regio- and endo-selectively and in good yields. The tricyclic skeleton has an anti-form built by the addition of ethyl vinyl ether from the less hindered side of homoquinones. All of the CH(3), Cl, Br, and CH(3)O substituents at the reacting C=C double bond afforded head-to-head (HH) addition predominantly. In the case of CH(3), Cl, and Br, the ethoxy group was oriented in the endo-position, while the CH(3)O substituent led to a 1/5 mixture with the exo-isomer. It was also found that the Br-substituted [2 + 2] adducts undergo a facile skeletal rearrangement, being converted into dihydro-o-benzoquinone monomethide derivatives for para-substitution and dihydrobenzofuran derivatives for meta-substitution, probably under the influence of the in situ generated HBr. Intramolecular [2 + 2] photocycloaddition of an alkenylhomobenzoquinone afforded a tetracyclic dione.

9.
Cortex ; 37(2): 187-95, 2001 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-11394720

RESUMEN

We report a left-handed man who demonstrated a pure agraphia limited to words written in Kana characters (syllabograms) following a right putaminal hemorrhage. Writing words in Kanji characters (logograms) was well preserved. His performance in Kana writing was characterized by intact ability to write single syllables, error increase in the second half of words directly proportional to the word length and correct but slow writing of words using kana blocks. Errors were more prominent in Hiragana words than Katakana words which are usually used to transcribe foreign words. Acoustic-grapheme sequencing per se was not impaired as shown by his correct performance in arranging character blocks. These findings suggest selective damage to the graphemic buffer, a module that temporarily maintains the graphemic representation elaborated in previous stages before it is sent to the peripheral systems for its motor realization.


Asunto(s)
Agrafia/psicología , Hemorragia Cerebral/psicología , Anciano , Agrafia/etiología , Afasia/psicología , Hemorragia Cerebral/complicaciones , Humanos , Masculino , Pruebas Neuropsicológicas , Paresia/etiología , Lectura , Escritura
10.
J Antibiot (Tokyo) ; 48(12): 1430-4, 1995 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-8557599

RESUMEN

YM-47141 and YM-47142 are new elastase inhibitor produced by Flexibacter sp. Q17897. These structures were elucidated by MS and NMR spectral analysis. YM-47141 and YM-47142 were the cyclic peptides containing tricarbonyl moiety hydrated on the center carbonyl carbon in DMSO-d6.


Asunto(s)
Elastasa Pancreática/antagonistas & inhibidores , Péptidos Cíclicos/química , Inhibidores de Serina Proteinasa/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Péptidos Cíclicos/farmacología , Espectrometría de Masa Bombardeada por Átomos Veloces , Estereoisomerismo
11.
ASAIO J ; 40(3): M803-7, 1994.
Artículo en Inglés | MEDLINE | ID: mdl-8555625

RESUMEN

If the oxygen dissolved in seawater could be used for breathing, human beings could spend more time under water, greatly increasing their mobility. As yet, however, sufficient oxygen to enable us to live in the sea for long periods is not available. An artificial gill system has been developed for oxygen uptake from water to deoxygenated air using perfluorooctylbromide (PFOB), which has high oxygen solubility. It was found that oxygen was transferred rapidly from water to PFOB when water flowed outside the hollow fibers and PFOB flowed inside. Oxygen transfer through the membrane from PFOB to air was found to be the rate determining step. Use of PFOB gave a stable supply of oxygen from water to deoxygenated air over long periods. It was found that PFOB acts as a storage medium for oxygen.


Asunto(s)
Órganos Artificiales , Fluorocarburos , Branquias , Oxígeno/metabolismo , Aire , Animales , Ingeniería Biomédica , Estudios de Evaluación como Asunto , Branquias/fisiología , Humanos , Hidrocarburos Bromados , Técnicas In Vitro , Membranas Artificiales , Agua
12.
ASAIO J ; 45(5): 418-23, 1999.
Artículo en Inglés | MEDLINE | ID: mdl-10503618

RESUMEN

A great deal of research has been conducted focusing on membrane materials with reference to their blood compatibility, but blood compatibility is influenced both by the material used in membranes and their structure, and by the flow conditions at the membrane surface. Accordingly, the relationship between membrane surface roughness and hemocompatibility has been evaluated using five types of membranes of differing surface roughness by evaluating the inner surfaces of the hollow fibers by atomic force microscopy (AFM) and by measuring platelet adhesion ratios using bovine blood. The yield stress, which equates to flow characteristics, was also evaluated using a glycerol suspension of polymethylmethacrylate (PMMA), a Bingham fluid. It was found that membranes having rough surfaces had high platelet adhesion ratios and poor hemocompatibility, whereas those with smoother surfaces had lower platelet adhesion ratios and better hemocompatibility. Measurement of the yield stresses for these membranes revealed higher values for those with rough surfaces, and lower values for those with smoother polyethylene glycol (PEG) grafted surfaces. This suggests that flow conditions at the membrane surface differ according to its surface roughness, and that this difference in flow conditions also influences hemocompatibility.


Asunto(s)
Membranas Artificiales , Adhesividad Plaquetaria , Diálisis Renal , Celulosa , Humanos
13.
Kyobu Geka ; 50(3): 251-5, 1997 Mar.
Artículo en Japonés | MEDLINE | ID: mdl-9121035

RESUMEN

A 47-year-old female with localized fibrous pleural mesothelioma arising from the visceral pleura of S2 of rt-upper lobe is reported. We demonstrated an indirect radiographic sign of pedunculation which was the change in position of the mass with the change in patient's position. The tumor was completely extirpated with a partial resection of the adjacent lung tissues through thoracotomy. Histopathologically, the tumor consisted of collagen fibers and small amount of fibroblast. Localized fibrous mesothelioma was recognized as benign with good prognosis. However, a few cases showing recurrence after resection were reported among them. So it was considered that this patient should be followed up carefully.


Asunto(s)
Mesotelioma/patología , Neoplasias Pleurales/patología , Femenino , Humanos , Mesotelioma/diagnóstico por imagen , Mesotelioma/cirugía , Persona de Mediana Edad , Neoplasias Pleurales/diagnóstico por imagen , Neoplasias Pleurales/cirugía , Tomografía Computarizada por Rayos X
14.
Kyobu Geka ; 49(13): 1127-9, 1996 Dec.
Artículo en Japonés | MEDLINE | ID: mdl-8958693

RESUMEN

A 40-year old woman had experienced repeated spontaneous pneumothorax on the right side at the beginning of each menstrual period for one year. The level of serum CA125 was high at 60 ng/ml. Right thoracotomy revealed the presence of multiple darkly-pigmented spots and several pinhole like perforations near the central tendon of the diaphragm. The partial resection of the diaphragm was done, endometrial tissues were detected histologically. Tha patient has been asymptomatic after operation and the level of serum CA125 is normal.


Asunto(s)
Antígeno Ca-125/sangre , Menstruación , Neumotórax/inmunología , Adulto , Endometriosis/complicaciones , Endometriosis/patología , Femenino , Humanos , Neumotórax/cirugía
15.
Oncogene ; 29(22): 3263-75, 2010 Jun 03.
Artículo en Inglés | MEDLINE | ID: mdl-20228841

RESUMEN

HOP homeobox (HOPX) is an unusual homeobox gene encoding three spliced transcript variants, among which the only HOPX-beta promoter harbors CpG islands. The characteristics of its promoter methylation was analyzed using bisulfite sequencing and quantitative-methylation-specific polymerase chain reaction (Q-MSP), and the effects of HOPX expression were also examined. HOPX-beta expression was silenced in all gastric cancer cell lines tested; its expression could be restored by treatment with demethylating agent. On Q-MSP, HOPX-beta hypermethylation (cut-off value of 3.55) was found in 84% (67 out of 80) of primary tumor tissues and 10% (8 out of 80) of the corresponding normal tissues and could discriminate normal from tumor tissues (P<0.0001). The prognosis of the advanced cases with HOPX-beta hypermethylation was as poor as those with stage IV disease when cut-off value was set at 11.28. This finding was validated in an independent cohort of 90 advanced gastric cancers. The HOPX-beta hypermethylation was also an independent prognostic factor (P=0.029) on multivariate analysis. Exogenous HOPX expression significantly inhibited cell proliferation, colony formation and invasion as well as enhanced apoptosis. Taken together, HOPX-beta promoter methylation is a frequent and cancer-specific event in gastric cancer. Quantitative assessment of HOPX-beta methylation has great clinical potential as a marker of tumor aggressiveness.


Asunto(s)
Metilación de ADN , Genes Homeobox , Neoplasias Gástricas/genética , Procesos de Crecimiento Celular/genética , Línea Celular Tumoral , Estudios de Cohortes , Islas de CpG , Regulación Neoplásica de la Expresión Génica , Silenciador del Gen , Proteínas de Homeodominio/genética , Humanos , Inmunohistoquímica , Invasividad Neoplásica , Pronóstico , Regiones Promotoras Genéticas , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patología , Proteínas Supresoras de Tumor/genética
17.
Biosci Biotechnol Biochem ; 63(8): 1353-9, 1999 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10500997

RESUMEN

A new method is reported that can be performed within a single vessel to analyze the composition of aldose, hexosamine, and sialic acid residues of glycoproteins, glycolipids, and oligosaccharides. Glycoconjugates are treated with sialidase or subjected to mild acid hydrolysis, before being treated with N-acetylneuraminic acid aldolase to convert the free sialic acid residues to their corresponding N-acylmannosamines. The reaction mixture is then successively subjected to acid hydrolysis (in order to produce monosaccharides), N-acetylation, and conversion with p-aminobenzoic acid ethyl ester (ABEE). The ABEE-converted monosaccharides are simultaneously determined by reverse-phase high-performance liquid chromatography. Determination of the sugar compositions of bovine fetuin, II3NeuGc alpha-LacCer, and 3'-sialyllactose with this method was found to be highly accurate. Linearity of the peak area vs. the amount of bovine fetuin ranged from 1 to 50 micrograms in all ABEE-converted monosaccharides. With a slight modification to this method, sialic acid residues can be separately determined as NeuAc and NeuGc. This novel method and its modified version are used to demonstrate the sugar compositions of alpha 1-acid glycoproteins from several sources.


Asunto(s)
Glucolípidos/química , Glicoproteínas/química , Hexosaminas/análisis , Monosacáridos/análisis , Ácido N-Acetilneuramínico/análisis , Oligosacáridos/química , Acetilación , Animales , Bovinos , Cromatografía Líquida de Alta Presión , Perros , Humanos , Hidrólisis , Modelos Lineales , Oxo-Ácido-Liasas/química , Ovinos
18.
Org Lett ; 2(13): 1979-1981, 2000 Jun 29.
Artículo en Inglés | MEDLINE | ID: mdl-10891206

RESUMEN

The photoinduced electron transfer reaction of halogenated 1,4-benzoquinones with 2,3-dimethyl-2-butene or 3,4-dimethyl-2-pentene gave the primary, secondary, and tertiary monoallyl ethers of hydroquinones, with the product distributions highly dependent on the steric nature of quinones and olefins.

19.
Nihon Naibunpi Gakkai Zasshi ; 62(5): 662-71, 1986 May 20.
Artículo en Japonés | MEDLINE | ID: mdl-3089848

RESUMEN

The changes in serum prolactin levels during the menstrual cycle have not been clarified yet. The present study was conducted to investigate whether the changes in serum prolactin levels during the menstrual cycle exist in ovulatory infertile patients having high prolactin release due to TRH administration described below (transient hyperprolactinemia) and control cases. Serum prolactin levels in both groups were less than 25 ng/ml at daytime. In the patients with transient hyperprolactinemia, serum prolactin levels showed more than 150 ng/ml at 30 min. after the administration of 500 micrograms of TRH, and those were less than 150 ng/ml in the normal control group. The daily changes of serum FSH, LH, prolactin, estradiol and progesterone levels were determined by radioimmunoassay in 6 cases of transient hyperprolactinemia and 5 controls which showed normal ovulatory cycles in the patterns of the BBT charts and other hormones. An estrogen feedback test was also carried out at the mid-luteal phase in 9 cases of transient hyperprolactinemia and the controls to determine serum levels of FSH, LH, prolactin and estradiol. In the patients with transient hyperprolactinemia, 5 mg of bromocriptine was administered every day for more than 30 days, and the effects of bromocriptine on the changes in serum gonadotropin levels by the estrogen feedback test were also analysed. Serum prolactin levels in the follicular, ovulatory and mid-luteal phases increased significantly in the patients with transient hyperprolactinemia, compared to the controls (p less than 0.005). The pattern of serum prolactin levels in the patients with transient hyperprolactinemia was almost synchronized with that of serum estradiol levels. There was also a significant correlation (r = 0.5782, p less than 0.005) between the prolactin and estradiol levels in the serum of the patients. The obvious change was not noted in serum prolactin levels during the menstrual cycle of the controls. No significant change in other hormones was observed during the cycle between these two groups. After the administration of estradiol benzoate (100 micrograms/kg), serum estradiol levels increased markedly, serum prolactin levels increased with the similar change in serum estradiol levels, and serum prolactin levels in the patients with transient hyperprolactinemia were significantly higher compared to those of the controls (p less than 0.01 approximately 0.005). The responses of serum gonadotropin levels by the administration of estradiol benzoate had good positive and negative feedback effects in the patients with transient hyperprolactinemia as well as those of the control group.(ABSTRACT TRUNCATED AT 400 WORDS)


Asunto(s)
Hiperprolactinemia/sangre , Infertilidad Femenina/sangre , Prolactina/metabolismo , Adulto , Estradiol/sangre , Femenino , Hormona Folículo Estimulante/sangre , Humanos , Fase Luteínica , Hormona Luteinizante/sangre , Ciclo Menstrual , Ovulación , Progesterona/sangre
20.
Nihon Naibunpi Gakkai Zasshi ; 62(2): 117-25, 1986 Feb 20.
Artículo en Japonés | MEDLINE | ID: mdl-3084307

RESUMEN

It is well known that the luteal function in the patients with hyperprolactinemia is much suppressed by high level of serum prolactin. Present study was performed to investigate whether the luteal function in the patients with transient or occulted hyperprolactinemia was affected by the transient increase of serum prolactin level. The circadian changes of serum FSH, LH, prolactin, estrone, estradiol and progesterone levels were examined in seven cases of the transient or occulted hyperprolactinemia whose BBT charts showed biphasic patterns. Serum prolactin levels of these patients were less than 25 ng/ml at daytime and more than 150 ng/ml at 30 minutes after the administration of 500 micrograms of TRH. Blood samplings were taken every two hours through an intravenous indwelling catheter without any disturbances. All of the patients had their breakfast at 7 to 8, lunch at 11 to 12 and dinner at 17 to 18 o'clock and slept from 22 until 6 in the next morning. Serum FSH, LH, prolactin, estrone, estradiol and progesterone levels were determined by RIA and the circadian changes of these hormones were analysed. Then, 5 mg of bromocriptine was administered every day to these patients for more than 30 days and the duration of the luteal phase and the mid-luteal serum estradiol and progesterone levels for the indicators of the luteal function were examined before and after the administration of bromocriptine. The circadian changes of serum prolactin levels in the patients showed significant increase during both daytime and night compared to those of the control (p less than 0.005, p less than 0.005).(ABSTRACT TRUNCATED AT 250 WORDS)


Asunto(s)
Cuerpo Lúteo/fisiopatología , Hiperprolactinemia/fisiopatología , Adulto , Bromocriptina/farmacología , Ritmo Circadiano , Estradiol/sangre , Estrona/sangre , Femenino , Hormona Folículo Estimulante/sangre , Humanos , Hiperprolactinemia/sangre , Progesterona/sangre , Prolactina/sangre
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