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1.
J Photochem Photobiol B ; 104(1-2): 285-91, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-21393013

RESUMEN

The effective fluorescence cross-section of photosystem 2 (PS2) was defined by measurements of chlorophyll a fluorescence induction curves for the wild type of the unicellular cyanobacterium Synechocystis sp. PCC6803, C-phycocyanin deficient mutant (CK), and mutant that totally lacks phycobilisomes (PAL). It was shown that mutations lead to a strong decrease of the PS2 effective fluorescence cross-section. For instance, the effective fluorescence cross-section of PS2 for wild type, CK and PAL mutants excited at λ(ex)=655 nm were found to be 896, 220 and 83 Å(2) respectively. Here we present an estimation of energy transfer efficiency from phycobilisomes to the pigment-protein complexes of PS2. It was shown that the PS2 fluorescence enhancement coefficient reaches a maximum value of 10.7 due to the energy migration from phycobilisomes. The rate constant of energy migration was found to be equal to 1.04 × 10(10) s(-1).


Asunto(s)
Complejo de Proteína del Fotosistema II/metabolismo , Ficocianina/metabolismo , Synechocystis/metabolismo , Clorofila/química , Clorofila/metabolismo , Clorofila A , Transferencia de Energía , Mutación , Complejo de Proteína del Fotosistema II/química , Ficobilisomas/metabolismo , Ficocianina/genética , Espectrometría de Fluorescencia , Synechocystis/enzimología , Synechocystis/genética
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