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Anthropogenic climate change has resulted in warming temperatures and reduced oxygen concentrations in the global oceans. Much remains unknown on the impacts of reduced oxygen concentrations on the biology and distribution of marine fishes. In the Southern California Channel Islands, visual fish surveys were conducted frequently in a manned submersible at three rocky reefs between 1995 and 2009. This area is characterized by a steep bathymetric gradient, with the surveyed sites Anacapa Passage, Footprint and Piggy Bank corresponding to depths near 50, 150 and 300 m. Poisson models were developed for each fish species observed consistently in this network of rocky reefs to determine the impact of depth and year on fish peak distribution. The interaction of depth and year was significant in 23 fish types, with 19 of the modelled peak distributions shifting to a shallower depth over the surveyed time period. Across the 23 fish types, the peak distribution shoaled at an average rate of 8.7 m of vertical depth per decade. Many of the species included in the study, including California sheephead, copper rockfish and blue rockfish, are targeted by commercial and recreational fisheries. CalCOFI hydrographic samples are used to demonstrate significant declines in dissolved oxygen at stations near the survey sites which are forced by a combination of natural multidecadal oscillations and anthropogenic climate change. This study demonstrates in situ fish depth distribution shifts over a 15-year period concurrent with oxygen decline. Climate-driven distribution shifts in response to deoxygenation have important implications for fisheries management, including habitat reduction, habitat compression, novel trophic dynamics and reduced body condition. Continued efforts to predict the formation and severity of hypoxic zones and their impact on fisheries dynamics will be essential to guiding effective placement of protected areas and fisheries regulations.
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Peces , Oxígeno , Animales , Arrecifes de Coral , Ecosistema , Explotaciones Pesqueras , Océanos y MaresRESUMEN
There are currently thousands of offshore platforms in place for oil and gas extraction worldwide, and decommissioning efforts over the next three decades are estimated to cost more than US$200 billion. As platforms reach the end of their useful lifetime, operators and regulatory agencies will assess the environmental impact of potential decommissioning strategies. Among the many factors that will be weighed in preparation for these major economic and engineering challenges is the fate of the fish and invertebrate communities that inhabit the structures underwater. Offshore platforms act as inadvertent artificial reefs, and some are recognized among the most productive fish habitats in the global oceans. We present a model for forecasting changes to fish communities surrounding offshore installations following a series of decommissioning alternatives. Using 24 platforms off southern California, we estimate fish biomass and somatic production under three possible decommissioning scenarios: leave in place, partial removal at 26-m depth, and complete removal of the platform and underlying shell mound. We used fish density and size data from scuba and submersible surveys of the platforms from 1995-2013 to estimate biomass and annual somatic production. Bottom trawl surveys were used to characterize future fish assemblages at platform sites under the complete-removal decommissioning scenario. Based on a conservatively modeled extrapolation of the survey data, we found that complete removal of a platform resulted in 95% or more reduction in the average fish biomass and annual somatic production at the site, while partial removal resulted in far smaller losses, averaging 10% or less. In the event that all surveyed platforms are completely removed, we estimated a total loss of more than 28,000 kg of fish biomass in the Southern California Bight. Platform habitats, which attract reef-dwelling fish species, had minimal overlap in community composition with the surrounding soft-bottom habitat. To best serve the wide range of stakeholder interests, the site-specific biomass, productivity and species composition information provided in this study should be incorporated into strategic decommissioning planning. This approach could be used as a model for informing "rigs to reefs" discussions occurring worldwide.
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Peces , Yacimiento de Petróleo y Gas , Animales , Ecosistema , Ambiente , InvertebradosRESUMEN
Foundation species define the ecosystems they live in, but ecologists have often characterized dominant plants as foundational without supporting evidence. Giant kelp has long been considered a marine foundation species due to its complex structure and high productivity; however, there is little quantitative evidence to evaluate this. Here, we apply structural equation modelling to a 15-year time series of reef community data to evaluate how giant kelp affects the reef community. Although species richness was positively associated with giant kelp biomass, most direct paths did not involve giant kelp. Instead, the foundational qualities of giant kelp were driven mostly by indirect effects attributed to its dominant physical structure and associated engineering influence on the ecosystem, rather than by its use as food by invertebrates and fishes. Giant kelp structure has indirect effects because it shades out understorey algae that compete with sessile invertebrates. When released from competition, sessile species in turn increase the diversity of mobile predators. Sea urchin grazing effects could have been misinterpreted as kelp effects, because sea urchins can overgraze giant kelp, understorey algae and sessile invertebrates alike. Our results confirm the high diversity and biomass associated with kelp forests, but highlight how species interactions and habitat attributes can be misconstrued as direct consequences of a foundation species like giant kelp.
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Biodiversidad , Ecosistema , Peces/fisiología , Invertebrados/fisiología , Macrocystis/fisiología , Animales , Cadena AlimentariaRESUMEN
Identifying spatial scales of variation in natural communities and the processes driving them is critical for obtaining a predictive understanding of biodiversity. In this study, we focused on diverse communities inhabiting productive kelp forests on shallow subtidal rocky reefs in southern California, USA. We combined long-term community surveys from 86 sites with detailed environmental data to determine what structures assemblages of fishes, invertebrates and algae at multiple spatial scales. We identified the spatial scales of variation in species composition using a hierarchical analysis based on eigenfunctions, and assessed how sea surface temperature (SST), water column chlorophyll, giant kelp biomass, wave exposure and potential propagule delivery strength contributed to community variation at each scale. Spatial effects occurring at multiple scales explained 60% of the variation in fish assemblages and 52% of the variation in the assemblages of invertebrates and algae. Most variation occurred over broad spatial scales (> 200 km) consistent with spatial heterogeneity in SST and potential propagule delivery strength, while the latter also explained community variation at medium scales (65-200 km). Small scale (1-65 km) community variation was substantial but not linked to any of the measured drivers. Conclusions were consistent for both reef fishes and benthic invertebrates and algae, despite sharp differences in their adult mobility. Our results demonstrate the scale dependence of environmental drivers on kelp forest communities, showing that most species were strongly sorted along oceanographic conditions over various spatial scales. Such spatial effects must be integrated into models assessing the response of marine ecosystems to climate change.
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Kelp , Animales , Biodiversidad , California , Ecosistema , BosquesRESUMEN
OBJECTIVE: To determine the frequency distribution and antibiotic resistance of pathogens isolated from the cerebrospinal fluid samples of children with bacterial meningitis (BM) and to provide a basis for the timely and effective treatment of childhood BM. METHODS: Retrospective analysis was performed on pathogens isolated from 5097 cerebrospinal fluid samples collected from children in Kunming Children's Hospital between January 2008 and June 2012, as well as drug sensitivity test results. Kirby-Bauer antibiotic testing was used to analyze the sensitivity of these pathogens to commonly used antibiotics. RESULTS: A total of 116 pathogen strains were detected from the 5097 cerebrospinal fluid samples, including 77 (66.4%) Gram-positive strains, 30 (25.9%) Gram-negative strains, and 9 (7.8%) fungal strains, with a positive rate of 2.28%. The six most frequently isolated pathogens were Staphylococcus epidermidis (32 strains, 27.6%), Streptococcus pneumoniae (15 strains, 12.9%), Escherichia coli (15 strains, 12.9%), Staphylococcus haemolyticus (9 strains, 7.8%), Cryptococcus neoformans (8 strains, 6.9%) and Staphylococcus aureus (6 strains, 5.2%). Coagulase-negative staphylococci was the predominant pathogen in neonates and young infants with BM, and its sensitivity rates to penicillin, erythromycin and clindamycin were lower than 40%. Streptococcus pneumoniae had a penicillin sensitivity rate of 13.4%, while sensitivity rates to erythromycin and clindamycin reached 60.0%. No Staphylococcus and Streptococcus pneumoniae pathogens resistant to vancomycin were found. Gram-negative bacilli had relatively high sensitivity rates to imipenem, meropenem, cefoperazone/sulbactam and cefepime. CONCLUSIONS: Gram-positive cocci are the predominant pathogens for childhood BM over the past five years. The detected pathogens develop high resistance to commonly used antibiotics. To prevent misdiagnosis, careful attention should be paid to BM caused by Cryptococcus neoformans.
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Meningitis Bacterianas/tratamiento farmacológico , Niño , Preescolar , Farmacorresistencia Bacteriana , Femenino , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Gramnegativas/aislamiento & purificación , Cocos Grampositivos/efectos de los fármacos , Cocos Grampositivos/aislamiento & purificación , Humanos , Lactante , Recién Nacido , Masculino , Meningitis Bacterianas/líquido cefalorraquídeo , Meningitis Bacterianas/microbiología , Estudios RetrospectivosRESUMEN
The severity of marine heatwaves (MHWs) that are increasingly impacting ocean ecosystems, including vulnerable coral reefs, has primarily been assessed using remotely sensed sea-surface temperatures (SSTs), without information relevant to heating across ecosystem depths. Here, using a rare combination of SST, high-resolution in-situ temperatures, and sea level anomalies observed over 15 years near Moorea, French Polynesia, we document subsurface MHWs that have been paradoxical in comparison to SST metrics and associated with unexpected coral bleaching across depths. Variations in the depth range and severity of MHWs was driven by mesoscale (10s to 100s of km) eddies that altered sea levels and thermocline depths and decreased (2007, 2017 and 2019) or increased (2012, 2015, 2016) internal-wave cooling. Pronounced eddy-induced reductions in internal waves during early 2019 contributed to a prolonged subsurface MHW and unexpectedly severe coral bleaching, with subsequent mortality offsetting almost a decade of coral recovery. Variability in mesoscale eddy fields, and thus thermocline depths, is expected to increase with climate change, which, along with strengthening and deepening stratification, could increase the occurrence of subsurface MHWs over ecosystems historically insulated from surface ocean heating by the cooling effects of internal waves.
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Antozoos , Ecosistema , Animales , Blanqueamiento de los Corales , Agua de Mar , Arrecifes de CoralRESUMEN
OBJECTIVE: To analyze 43 leukemia genes in children with acute lymphoblastic leukemia (ALL) in Yunnan province, and provide the basis for the diagnosis and treatment of children with ALL in this area. METHODS: The clinical data of 428 children with newly diagnosed ALL in Yunnan area from January 2015 to December 2020 were retrospectively analyzed. Multiple nested PCR technology was used to detect 43 common leukemia genes. RESULTS: Among the 428 children with ALL, 159 were positive for leukemia genes, with a positive rate of 37.15% (159/428), and a total of 15 leukemia genes were detected. Among the 159 leukemia gene-positive children, ETV6-RUNX1+ accounted for 25.79% (41/159), followed by E2A-PBX1+ and BCR-ABL+, accounting for 24.53% (39/159) and 23.27% (37/159) respectively. MLL+ accounted for 6.29% (10/159), WT1+ accounted for 4.40% (7/159), IKZF1 gene deletion and CRLF2+ accounted for 3.77% (6/159) respectively. The positive rate of MLL (46.15%) was the highest in ï¼1-year old group, the positive rate of ETV6-RUNX1 (10.56%) was the highest in 1-10-year old group, and BCR-ABL+ rate (23.65%) was the highest in >10-year old group. The distribution of leukemia genes in different age groups was statistically significant (P <0.05). CONCLUSION: The most common fusion gene of children with ALL in Yunnan is ETV6-RUNX1, followed by E2A-PBX1 and BCR-ABL.
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Proteínas de Fusión Oncogénica , Leucemia-Linfoma Linfoblástico de Células Precursoras , Niño , Humanos , Lactante , Preescolar , Proteínas de Fusión Oncogénica/genética , Proteínas de Fusión bcr-abl/genética , Subunidad alfa 2 del Factor de Unión al Sitio Principal/genética , Estudios Retrospectivos , China , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , GenotipoRESUMEN
Data support knowledge development and theory advances in ecology and evolution. We are increasingly reusing data within our teams and projects and through the global, openly archived datasets of others. Metadata can be challenging to write and interpret, but it is always crucial for reuse. The value metadata cannot be overstated-even as a relatively independent research object because it describes the work that has been done in a structured format. We advance a new perspective and classify methods for metadata curation and development with tables. Tables with templates can be effectively used to capture all components of an experiment or project in a single, easy-to-read file familiar to most scientists. If coupled with the R programming language, metadata from tables can then be rapidly and reproducibly converted to publication formats including extensible markup language files suitable for data repositories. Tables can also be used to summarize existing metadata and store metadata across many datasets. A case study is provided and the added benefits of tables for metadata, a priori, are developed to ensure a more streamlined publishing process for many data repositories used in ecology, evolution, and the environmental sciences. In ecology and evolution, researchers are often highly tabular thinkers from experimental data collection in the lab and/or field, and representations of metadata as a table will provide novel research and reuse insights.
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Patients with metastatic renal cell cancer (mRCC) for whom surgery is ineffective may experience a poor prognosis. The different sites where cancer has spread, and the different ways to treat it in the immune checkpoint inhibitors era could help clinical decision-making. In this study, individuals with mRCC were selected from the SEER database between 2015 and 2016 based on the Food and Drug Administration (FDA) approval of ICIs. A total of 4011 mRCC patients were studied (2239 with lung metastasis vs. 797 with liver metastasis in the immune checkpoint inhibitors period). The age ≤ 64 years and male were the majority in all cases of mRCC. When the two groups (lung metastasis and liver metastasis) were compared, the liver metastasis group had more bone metastasis than the lung metastasis group (41.8% vs. 34.1%, p < 0.001), but the lung metastasis group had more brain metastasis (8.9% vs. 11.5%) (p = 0.023). In a study of overall survival (OS) in the ICI era for mRCC, we found that lung metastasis was significantly associated with improved survival compared to liver metastasis (p < 0.001: 7 months vs. 4 months). This survival advantage restricted in lung metastasis group of mRCC after adjusting age, sex, race, marital status, histological type, metastasis to bone, and brain, origin, radiotherapy record chemotherapy record, surgery on multivariable using Cox proportional hazard model (HR = 1.407; 95% CI = 1. 269-1.560; p < 0.001). The overall survival difference between the variables of the lung metastasis and liver metastasis was noted among most of the variables, with survival benefits restricted to patients in lung metastasis in the ICI era. Patients who had undergone chemotherapy and surgery were strongly positive predictors for better OS (HR = 0.427; 95% CI = 0.379-0.481; p < 0.001) (HR = 0.371; 95% CI = 0.311-0.444; p=< 0.001), and (HR = 0.313; 95% CI = 0.264-0.372; p < 0.001), (HR = 0.427; 95% CI = 0.320-0.568; p < 0.001) in lung metastasis group and liver metastasis group. The c-index of the prognostic nomogram for OS prediction was 0.74 and 0.73. This study found that patients with lung metastasis who received ICI had better survival than those with liver metastasis. Chemotherapy and surgery enhanced survival in kidney cancer patients, whereas radiation had little impact. We developed a complete and realistic nomogram for mRCC patients based on distant metastases to the lung and liver.
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Offshore oil and gas platforms have a finite life of production operations. Once production ceases, decommissioning options for the platform are assessed. The role that a platform's jacket plays as fish habitat can inform the decommissioning decision. In this study, conducted along the crossbeams of a California platform jacket and using an ROV, we compared estimates of fish diversity and densities determined from a targeted "biological" survey with those from a replicated "structural" survey. We found that the water column fish species assemblages characterized by the two methods were similar. By contrast, the two survey methods yielded different species assemblages inhabiting the crossbeam at the platform jacket base. This difference occurred because, at least off California, the platform jacket base species diversity tends to be highest where the bottom crossbeam is undercut, creating sheltering sites for many species. Because the structural method inadequately imaged the seafloor-crossbeam interface, particularly where a gap occurred between crossbeam and seafloor, substantial numbers of fishes were not visible. While we cannot extrapolate from this study to all platforms' worldwide, it is clear that routine platform structural integrity surveys may be a valuable source for opportunistic marine community surveys. Intentional planning of the structural survey to incorporate relatively minor variations (e.g., maintaining fixed ROV distance from the infrastructure and consistent 90° camera angle) coupled with a deliberate consideration of the platform ecology (e.g., positioning the ROV to capture the seafloor-crossbeam interface) can substantially improve the effects on fish assemblage assessments from routine structural surveys without compromising the integrity assessment. We suggest that these biases should be both acknowledged and, understood when using routine structural surveys to inform platform ecology assessment. Additional consideration may be given to structural surveys that incorporate incremental adjustments to provide better data applicability to biological assessments.
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Monitoreo del Ambiente/métodos , Robótica/métodos , Animales , Biodiversidad , California , Ecosistema , Peces/clasificación , Yacimiento de Petróleo y Gas , Densidad de Población , Encuestas y Cuestionarios/estadística & datos numéricos , AguaRESUMEN
OBJECTIVE: To investigate the epidemic situation and its epidemic characteristics of malaria in Qingdao City from 2012 to 2017, so as to provide the evidence for the prevention and control of malaria in this city. METHODS: The data of cases and epidemiological characteristics of malaria in Qingdao City, 2012-2017 were collected from the China's disease prevention and control system infectious disease report information management system and parasitic disease prevention and control information management system, and analyzed. RESULTS: A total of 103 cases of malaria were reported in Qingdao City from 2012 to 2017, and all of them were imported. Among them, 98 (95.15%) were imported from Africa and 5 (4.85%) from Asia. There were 92 laboratory confirmed cases (89.32%) and 11 (10.68%) clinically diagnosed cases. The highest incidence occurred in 2016 (0.28/105) . According to the classification of cases, there were 87 falciparum malaria cases (84.47%), 13 vivax malaria cases (12.62%), and 3 ovale malaria cases (2.91%) . These patients were mainly young men, and the male to female ratio was 19.6â¶1. There were malaria case reports in each month, and the peak onset season was not obvious. Newly diagnosed and confirmed units were mainly municipal medical institutions; the median of onset-definite diagnosis was 4 days. There was one death case, but there were no recurrence, reburning, and imported secondary cases. CONCLUSIONS: There were no local malaria cases in Qingdao City from 2012 to 2017. The prevention and control of imported malaria is the focus of the future work.
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Malaria , Plasmodium , África , Asia , China/epidemiología , Enfermedades Transmisibles Importadas/epidemiología , Enfermedades Transmisibles Importadas/parasitología , Femenino , Humanos , Malaria/epidemiología , Malaria/parasitología , Malaria/prevención & control , MasculinoRESUMEN
This study investigated the effects of chitooligosaccharide on lipid metabolism, immune organ development, and lymphocyte apoptosis in broiler chickens. A total of 480 one-day-old broiler chickens (Arbor Acres) were randomly and evenly assigned to control group and experimental groups I, II, and III. The control group was given a basic diet, while experimental groups I, II, and III were given basic diets for 42 days, supplemented with 50 mg/kg chlortetracycline, 20, and 40 mg/kg chitooligosaccharide, respectively. We found levels of serum triglycerides (TG) and low density lipoprotein cholesterol (LDL-C) to be significantly reduced in experimental group II after 21 days, while the periarterial lymphatic sheath area of the spleens and the average number of bursa of Fabricius nodes were markedly increased. The serum total protein (TP) and high density lipoprotein cholesterol (HDL-C) levels, bursa of Fabricius index, and bursa of Fabricius lobule areas were additionally increased in experimental group III. After 42 days, the serum TP content had also increased and the bursa of Fabricius lobule area was augmented as well in experimental group II. Moreover, the splenic periarterial lymphatic sheat areas and the average numbers of bursa of Fabricius nodes were significantly increased in experimental group III. At both 21 and 42 days, numbers of Caspase 3-positive cells in spleen and bursa of Fabricius were significantly decreased in experimental groups II and III. Our results show that appropriate supplementation of chitooligosaccharide may improve lipid metabolism, promote immune organ development, and inhibit lymphocyte apoptosis in broilers.
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Bolsa de Fabricio/efectos de los fármacos , Quitina/análogos & derivados , Lípido A/sangre , Metabolismo de los Lípidos/efectos de los fármacos , Bazo/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Bolsa de Fabricio/crecimiento & desarrollo , Pollos , Quitina/farmacología , Quitosano , Clortetraciclina/farmacología , Linfocitos/efectos de los fármacos , Oligosacáridos , Bazo/crecimiento & desarrolloAsunto(s)
COVID-19 , Educación Médica , Estudiantes de Medicina , Empatía , Humanos , Pandemias , SARS-CoV-2RESUMEN
AIM: Mast cells are important in experimental diabetes. Plasma levels of immunoglobulin E (IgE), tryptases, and chymases are inflammatory markers of human diabetes. Whether they also correlate with the risk of pre-diabetes, however, remains unknown. METHODS AND RESULTS: A total of 260 subjects 55-75 years of age were grouped as normal glucose tolerance (NGT), isolated impaired fasting glucose (I-IFG), isolated impaired glucose tolerance (I-IGT), and mixed IFG/IGT. There were significant differences in plasma levels of high-sensitivity C-reactive protein (hsCRP) (P < 0.001) and IgE (P = 0.003) among all subgroups of pre-diabetes, and chymase in I-IGT (P = 0.043) and mixed IFG/IGT (P = 0.037) subgroups compared with NGT group. High-sensitivity CRP was a risk factor in all subgroups of pre-diabetes; IgE was a risk factor of mixed IFG/IGT; and chymase was a risk factor of I-IGT and mixed IFG/IGT. Interactions between hsCRP and high waist circumference (WC), waist-to-hip ratio (WHR), or HOMA-ß index, and interactions between IgE and high WC or tryptase levels all increased further the risk of developing I-IFG, I-IGT, or mixed IFG/IGT. CONCLUSION: Plasma hsCRP, IgE, and chymase levels associate with pre-diabetes status. While hsCRP, IgE, and chymase are individual risk factors of pre-diabetes, interactions with metabolic parameters increased further the risk of pre-diabetes.
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Quimasas/sangre , Prueba de Tolerancia a la Glucosa , Inmunoglobulina E/sangre , Estado Prediabético/sangre , Triptasas/sangre , Anciano , Glucemia/análisis , Proteína C-Reactiva/análisis , Ayuno , Femenino , Homeostasis , Humanos , Hipertensión/epidemiología , Células Secretoras de Insulina/fisiología , Modelos Logísticos , Masculino , Persona de Mediana Edad , Factores de Riesgo , Factores Sexuales , Circunferencia de la Cintura , Relación Cintura-CaderaRESUMEN
BACKGROUND: Recent studies have suggested that mast-cell activation and inflammation are important in obesity and diabetes. Plasma levels of mast cell proteases and the mast cell activator immunoglobulin E (IgE) may serve as novel inflammatory markers that associate with the risk of pre-diabetes and diabetes mellitus. METHODS AND RESULTS: A total of 340 subjects 55 to 75 years of age were grouped according to the American Diabetes Association 2003 criteria of normal glucose tolerance, pre-diabetes, and diabetes mellitus. The Kruskal-Wallis test demonstrated significant differences in plasma IgE levels (Pâ=â0.008) among groups with different glucose tolerance status. Linear regression analysis revealed significant correlations between plasma levels of chymase (Pâ=â0.030) or IgE (Pâ=â0.022) and diabetes mellitus. Ordinal logistic regression analysis showed that IgE was a significant risk factor of pre-diabetes and diabetes mellitus (odds ratio [OR]: 1.674, Pâ=â0.034). After adjustment for common diabetes risk factors, including age, sex, hypertension, body-mass index, cholesterol, homeostatic model assessment (HOMA) index, high-sensitivity C-reactive protein (hs-CRP), and mast cell chymase and tryptase, IgE remained a significant risk factor (OR: 1.866, Pâ=â0.015). Two-variable ordinal logistic analysis indicated that interactions between hs-CRP and IgE, or between IgE and chymase, increased further the risks of developing pre-diabetes and diabetes mellitus before (OR: 2.204, Pâ=â0.044; OR: 2.479, Pâ=â0.033) and after (OR: 2.251, Pâ=â0.040; OR: 2.594, Pâ=â0.026) adjustment for common diabetes risk factors. CONCLUSIONS: Both IgE and chymase associate with diabetes status. While IgE and hs-CRP are individual risk factors of pre-diabetes and diabetes mellitus, interactions of IgE with hs-CRP or with chymase further increased the risk of pre-diabetes and diabetes mellitus.
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Quimasas/sangre , Diabetes Mellitus/sangre , Diabetes Mellitus/enzimología , Inmunoglobulina E/sangre , Estado Prediabético/sangre , Estado Prediabético/enzimología , Triptasas/sangre , Anciano , Antropometría , Proteína C-Reactiva/metabolismo , Quimasas/metabolismo , Diabetes Mellitus/inmunología , Femenino , Humanos , Modelos Logísticos , Masculino , Mastocitos/enzimología , Persona de Mediana Edad , Estado Prediabético/inmunología , Factores de Riesgo , Triptasas/metabolismoRESUMEN
AIM: To examine the effects of different temperature protection on measures on preservation damages in liquid blood and explore the corresponding. METHODS: Take equal half blood samples from 10 healthy blood donors and divided each sample into two groups, put the fresh blood into CP2D-A solution at 0 degrees C and 4 degrees C, respectively and take the samples 21 days and 42 days, later and then measured the contents of membrane phospholipids with shafig-UR-rehman method, CaM with purification PED test, LPO with spectrophotometry. RESULTS: At the same temperature, when the preservation time was prolonged, peroxidation was increased, the preservation damages were also augmented; the damages were declined when the temperature was lower during the same period, the aging of blood was more evident at 4 degrees C. CONCLUSION: Blood peroxidation temperature is lower. The author pointed out the questions and prospects of blood preservation.
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Conservación de la Sangre/métodos , Membrana Eritrocítica , Temperatura , Adulto , Donantes de Sangre , Deformación Eritrocítica , Femenino , Humanos , Masculino , Factores de TiempoRESUMEN
AIM: To explore the effects of different temperature and time on preservation damages in liquid blood. METHODS: Take blood sample from 10 healthy blood donors, put the fresh blood into CP2D-A liquid at 0 degrees C and 4 degrees C, and take the samples after 1 week, 2 weeks and 3 weeks, and then measured the contents of GSH-Px, TSH, LPO, the contracting protein of RBC, and membrane fluidity. RESULTS: At the same temperature, when the preservation time is prolonged, peroxidation is increased, the preservation damages are also augmented; the damages are declined when the temperature is lower during the same period, the aging of blood was more evident at 4 degrees C. CONCLUSION: Blood peroxidation temperature is lower. The 0 degrees C group is better than 4 degrees C group.