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1.
Proc Natl Acad Sci U S A ; 120(6): e2212003120, 2023 02 07.
Artículo en Inglés | MEDLINE | ID: mdl-36719915

RESUMEN

While establishing an invasive infection, the dormant conidia of Aspergillus fumigatus transit through swollen and germinating stages, to form hyphae. During this morphotype transition, the conidial cell wall undergoes dynamic remodeling, which poses challenges to the host immune system and antifungal drugs. However, such cell wall reorganization during conidial germination has not been studied so far. Here, we explored the molecular rearrangement of Aspergillus fumigatus cell wall polysaccharides during different stages of germination. We took advantage of magic-angle spinning NMR to investigate the cell wall polysaccharides, without employing any destructive method for sample preparation. The breaking of dormancy was associated with a significant change in the molar ratio between the major polysaccharides ß-1,3-glucan and α-1,3-glucan, while chitin remained equally abundant. The use of various polarization transfers allowed the detection of rigid and mobile polysaccharides; the appearance of mobile galactosaminogalactan was a molecular hallmark of germinating conidia. We also report for the first time highly abundant triglyceride lipids in the mobile matrix of conidial cell walls. Water to polysaccharides polarization transfers revealed an increased surface exposure of glucans during germination, while chitin remained embedded deeper in the cell wall, suggesting a molecular compensation mechanism to keep the cell wall rigidity. We complement the NMR analysis with confocal and atomic force microscopies to explore the role of melanin and RodA hydrophobin on the dormant conidial surface. Exemplified here using Aspergillus fumigatus as a model, our approach provides a powerful tool to decipher the molecular remodeling of fungal cell walls during their morphotype switching.


Asunto(s)
Aspergillus fumigatus , Proteínas Fúngicas , Aspergillus fumigatus/metabolismo , Esporas Fúngicas/metabolismo , Proteínas Fúngicas/metabolismo , Polisacáridos/metabolismo , Quitina/metabolismo , Glucanos/metabolismo , Pared Celular/metabolismo
2.
J Cell Sci ; 135(5)2022 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-34878112

RESUMEN

Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (DHA-PLs) in physiology. Here, we investigated the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) triggered by RhoA inhibition-associated cell spreading. Lipidomic analyses showed that human umbilical vein endothelial cells (HUVECs) subjected to a DHA diet undergo a 6-fold enrichment in DHA-PLs at the plasma membrane (PM) at the expense of monounsaturated oleic acid-containing PLs (OA-PLs). Consequently, DHA-PL enrichment at the PM induces a reduction in cell thickness and shifts cellular membranes towards a permissive mode of membrane fusion for transcellular tunnel initiation. We provide evidence that a global homeostatic control of membrane tension and cell cortex rigidity minimizes overall changes of TEM area through a decrease of TEM size and lifetime. Conversely, low DHA-PL levels at the PM lead to the opening of unstable and wider TEMs. Together, this provides evidence that variations of DHA-PL levels in membranes affect cell biomechanical properties.


Asunto(s)
Ácidos Docosahexaenoicos , Fosfolípidos , Animales , Membrana Celular/metabolismo , Ácidos Docosahexaenoicos/metabolismo , Ácidos Docosahexaenoicos/farmacología , Células Endoteliales/metabolismo , Humanos , Fusión de Membrana , Fosfolípidos/metabolismo
3.
Int J Mol Sci ; 24(3)2023 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-36768366

RESUMEN

Mechanical properties of healthy and Dupuytren fibroblasts were investigated by atomic force microscopy (AFM). In addition to standard force curves, rheological properties were assessed using an oscillatory testing methodology, in which the frequency was swept from 1 Hz to 1 kHz, and data were analyzed using the structural damping model. Dupuytren fibroblasts showed larger apparent Young's modulus values than healthy ones, which is in agreement with previous results. Moreover, cell mechanics were compared before and after ML-7 treatment, which is a myosin light chain kinase inhibitor (MLCK) that reduces myosin activity and hence cell contraction. We employed two different concentrations of ML-7 inhibitor and could observe distinct cell reactions. At 1 µM, healthy and scar fibroblasts did not show measurable changes in stiffness, but Dupuytren fibroblasts displayed a softening and recovery after some time. When increasing ML-7 concentration (3 µM), the majority of cells reacted, Dupuytren fibroblasts were the most susceptible, not being able to recover from the drug and dying. These results suggested that ML-7 is a potent inhibitor for MLCK and that myosin II is essential for cytoskeleton stabilization and cell survival.


Asunto(s)
Citoesqueleto , Contractura de Dupuytren , Fibroblastos , Microscopía de Fuerza Atómica , Contracción Muscular , Cadenas Ligeras de Miosina , Humanos , Citoesqueleto/efectos de los fármacos , Citoesqueleto/metabolismo , Citoesqueleto/fisiología , Citoesqueleto/ultraestructura , Contractura de Dupuytren/tratamiento farmacológico , Contractura de Dupuytren/metabolismo , Contractura de Dupuytren/patología , Fibroblastos/efectos de los fármacos , Fibroblastos/metabolismo , Fenómenos Mecánicos , Cadenas Ligeras de Miosina/metabolismo , Quinasa de Cadena Ligera de Miosina/farmacología , Quinasa de Cadena Ligera de Miosina/uso terapéutico , Contracción Muscular/efectos de los fármacos , Contracción Muscular/fisiología
4.
EMBO J ; 37(21)2018 11 02.
Artículo en Inglés | MEDLINE | ID: mdl-30314966

RESUMEN

Although lysosomes perform a number of essential cellular functions, damaged lysosomes represent a potential hazard to the cell. Such lysosomes are therefore engulfed by autophagic membranes in the process known as lysophagy, which is initiated by recognition of luminal glycoprotein domains by cytosolic lectins such as Galectin-3. Here, we show that, under various conditions that cause injury to the lysosome membrane, components of the endosomal sorting complex required for transport (ESCRT)-I, ESCRT-II, and ESCRT-III are recruited. This recruitment occurs before that of Galectin-3 and the lysophagy machinery. Subunits of the ESCRT-III complex show a particularly prominent recruitment, which depends on the ESCRT-I component TSG101 and the TSG101- and ESCRT-III-binding protein ALIX Interference with ESCRT recruitment abolishes lysosome repair and causes otherwise reversible lysosome damage to become cell lethal. Vacuoles containing the intracellular pathogen Coxiella burnetii show reversible ESCRT recruitment, and interference with this recruitment reduces intravacuolar bacterial replication. We conclude that the cell is equipped with an endogenous mechanism for lysosome repair which protects against lysosomal damage-induced cell death but which also provides a potential advantage for intracellular pathogens.


Asunto(s)
Coxiella burnetii/metabolismo , Complejos de Clasificación Endosomal Requeridos para el Transporte/metabolismo , Lisosomas/metabolismo , Fiebre Q/metabolismo , Proteínas Sanguíneas , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Complejos de Clasificación Endosomal Requeridos para el Transporte/genética , Galectina 3/genética , Galectina 3/metabolismo , Galectinas , Células HeLa , Humanos , Lisosomas/genética , Lisosomas/patología , Fiebre Q/genética , Fiebre Q/patología
5.
PLoS Pathog ; 16(5): e1008106, 2020 05.
Artículo en Inglés | MEDLINE | ID: mdl-32463830

RESUMEN

Toxoplasma gondii possesses an armada of secreted virulent factors that enable parasite invasion and survival into host cells. These factors are contained in specific secretory organelles, the rhoptries, micronemes and dense granules that release their content upon host cell recognition. Dense granules are secreted in a constitutive manner during parasite replication and play a crucial role in modulating host metabolic and immune responses. While the molecular mechanisms triggering rhoptry and microneme release upon host cell adhesion have been well studied, constitutive secretion remains a poorly explored aspect of T. gondii vesicular trafficking. Here, we investigated the role of the small GTPase Rab11A, a known regulator of exocytosis in eukaryotic cells. Our data revealed an essential role of Rab11A in promoting the cytoskeleton driven transport of dense granules and the release of their content into the vacuolar space. Rab11A also regulates transmembrane protein trafficking and localization during parasite replication, indicating a broader role of Rab11A in cargo exocytosis at the plasma membrane. Moreover, we found that Rab11A also regulates extracellular parasite motility and adhesion to host cells. In line with these findings, MIC2 secretion was altered in Rab11A-defective parasites, which also exhibited severe morphological defects. Strikingly, by live imaging we observed a polarized accumulation of Rab11A-positive vesicles and dense granules at the apical pole of extracellular motile and invading parasites suggesting that apically polarized Rab11A-dependent delivery of cargo regulates early secretory events during parasite entry into host cells.


Asunto(s)
Vesículas Transportadoras/metabolismo , Vacuolas/metabolismo , Proteínas de Unión al GTP rab/metabolismo , Animales , Adhesión Celular , Línea Celular , Membrana Celular/metabolismo , Citoesqueleto/metabolismo , Interacciones Huésped-Parásitos/fisiología , Humanos , Proteínas de la Membrana/metabolismo , Microtúbulos/metabolismo , Parásitos/metabolismo , Transporte de Proteínas , Proteínas Protozoarias , Toxoplasma/metabolismo , Toxoplasmosis/metabolismo , Proteínas de Unión al GTP rab/fisiología
6.
Cell Microbiol ; 21(5): e12994, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-30552790

RESUMEN

If the mycelium of Aspergillus fumigatus is very short-lived in the laboratory, conidia can survive for years. This survival capacity and extreme resistance to environmental insults is a major biological characteristic of this fungal species. Moreover, conidia, which easily reach the host alveola, are the infective propagules. Earlier studies have shown the role of some molecules of the outer conidial layer in protecting the fungus against the host defense. The outer layer of the conidial cell wall, directly in contact with the host cells, consists of α-(1,3)-glucan, melanin, and proteinaceous rodlets. This study is focused on the global importance of this outer layer. Single and multiple mutants without one to three major components of the outer layer were constructed and studied. The results showed that the absence of the target molecules resulting from multiple gene deletions led to unexpected phenotypes without any logical additivity. Unexpected compensatory cell wall surface modifications were indeed observed, such as the synthesis of the mycelial virulence factor galactosaminogalactan, the increase in chitin and glycoprotein concentration or particular changes in permeability. However, sensitivity of the multiple mutants to killing by phagocytic host cells confirmed the major importance of melanin in protecting conidia.


Asunto(s)
Aspergillus fumigatus/metabolismo , Pared Celular/metabolismo , Melaninas/metabolismo , Esporas Fúngicas/metabolismo , Aspergilosis/inmunología , Aspergillus fumigatus/efectos de los fármacos , Aspergillus fumigatus/genética , Aspergillus fumigatus/patogenicidad , Azoles/farmacología , Bencenosulfonatos/farmacología , Caspofungina/farmacología , Pared Celular/efectos de los fármacos , Pared Celular/genética , Quitina/metabolismo , Rojo Congo/farmacología , Proteínas Fúngicas/metabolismo , Glucanos/genética , Glucanos/metabolismo , Glicoproteínas/genética , Glicoproteínas/metabolismo , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Melaninas/genética , Melaninas/fisiología , Monocitos/inmunología , Micelio/metabolismo , Fagocitos/metabolismo , Polisacáridos/metabolismo , Piocianina/farmacología , Esporas Fúngicas/citología , Esporas Fúngicas/genética , Factores de Virulencia/metabolismo
7.
EMBO Rep ; 19(1): 29-42, 2018 01.
Artículo en Inglés | MEDLINE | ID: mdl-29141986

RESUMEN

The interaction of Mycobacterium tuberculosis (Mtb) with pulmonary epithelial cells is critical for early stages of bacillus colonization and during the progression of tuberculosis. Entry of Mtb into epithelial cells has been shown to depend on F-actin polymerization, though the molecular mechanisms are still unclear. Here, we demonstrate that mycobacterial uptake into epithelial cells requires rearrangements of the actin cytoskeleton, which are regulated by ADP-ribosylation factor 1 (Arf1) and phospholipase D1 (PLD1), and is dependent on the M3 muscarinic receptor (M3R). We show that this pathway is controlled by Arf GTPase-activating protein 1 (ArfGAP1), as its silencing has an impact on actin cytoskeleton reorganization leading to uncontrolled uptake and replication of Mtb. Furthermore, we provide evidence that this pathway is critical for mycobacterial entry, while the cellular infection with other pathogens, such as Shigella flexneri and Yersinia pseudotuberculosis, is not affected. Altogether, these results reveal how cortical actin plays the role of a barrier to prevent mycobacterial entry into epithelial cells and indicate a novel role for ArfGAP1 as a restriction factor of host-pathogen interactions.


Asunto(s)
Citoesqueleto de Actina/metabolismo , Actinas/genética , Proteínas Activadoras de GTPasa/genética , Interacciones Huésped-Patógeno , Mycobacterium tuberculosis/patogenicidad , Alveolos Pulmonares/metabolismo , Células A549 , Factor 1 de Ribosilacion-ADP/genética , Factor 1 de Ribosilacion-ADP/metabolismo , Citoesqueleto de Actina/microbiología , Citoesqueleto de Actina/ultraestructura , Actinas/metabolismo , Proteínas Activadoras de GTPasa/antagonistas & inhibidores , Proteínas Activadoras de GTPasa/metabolismo , Regulación de la Expresión Génica , Humanos , Mycobacterium tuberculosis/fisiología , Fosfolipasa D/genética , Fosfolipasa D/metabolismo , Polimerizacion , Alveolos Pulmonares/microbiología , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/metabolismo , Receptor Muscarínico M3/genética , Receptor Muscarínico M3/metabolismo , Shigella flexneri/fisiología , Transducción de Señal , Especificidad de la Especie , Yersinia pseudotuberculosis/fisiología
8.
Biol Cell ; 111(3): 67-77, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30680759

RESUMEN

BACKGROUND: Bacterial invasion covers two steps: adhesion and entry per se. The cell signalling response is triggered upon pathogen interaction at the cell surface. This response continues when the pathogen is internalised. It is likely that these two steps activate different molecular machineries. So far, it has not been possible to easily follow in physiological conditions these events separately. We thus developed an approach to uncouple adhesion from entry using atomic force microscopy (AFM)-driven force and fluorescence measurements. RESULTS: We report nanometric-scale, high-resolution, functional dynamic measurements of bacterial interaction with the host cell surface using photonic and adhesion force analyses. We describe how to achieve a precise monitoring of iterative cell-bacterium interactions to analyse host cell signalling responses to infection. By applying this method to Yersinia pseudotuberculosis, we first unveil glycosylphosphatidylinositol-anchored protein domains recruitment to the bacterium cell surface binding site and concomitant cytoskeleton rearrangements using super-resolution fluorescence microscopy. Second, we demonstrate the feasibility of monitoring post-translationally modified proteins, for example, via ubiquitylation, during the first step of infection. CONCLUSION: We provide an approach to discriminate between cellular signalling response activated at the plasma membrane during host-pathogen interaction and that is triggered during the internalisation of the pathogen within the cell. SIGNIFICANCE: This approach adds to the technological arsenal to better understand and fight against pathogens and beyond the scope of microbiology to address conceptual issues of cell surface signalling.


Asunto(s)
Membrana Celular/metabolismo , Citosol/metabolismo , Yersinia pseudotuberculosis/metabolismo , Actinas/metabolismo , Adsorción , Sitios de Unión , Adhesión Celular , Glicosilfosfatidilinositoles/metabolismo , Proteínas Fluorescentes Verdes/metabolismo , Células HeLa , Humanos , Polimerizacion , Procesamiento Proteico-Postraduccional , Factor 6 Asociado a Receptor de TNF/metabolismo , Ubiquitinación
9.
J Cell Sci ; 130(18): 3009-3022, 2017 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-28743738

RESUMEN

Nonsense-mutation-containing messenger ribonucleoprotein particles (mRNPs) transit through cytoplasmic foci called P-bodies before undergoing nonsense-mediated mRNA decay (NMD), a cytoplasmic mRNA surveillance mechanism. This study shows that the cytoskeleton modulates transport of nonsense-mutation-containing mRNPs to and from P-bodies. Impairing the integrity of cytoskeleton causes inhibition of NMD. The cytoskeleton thus plays a crucial role in NMD. Interestingly, disruption of actin filaments results in both inhibition of NMD and activation of premature termination codon (PTC) readthrough, while disruption of microtubules causes only NMD inhibition. Activation of PTC readthrough occurs concomitantly with the appearance of cytoplasmic foci containing UPF proteins and mRNAs with nonsense mutations but lacking the P-body marker DCP1a. These findings demonstrate that in human cells, PTC readthrough occurs in novel 'readthrough bodies' and requires the presence of UPF proteins.


Asunto(s)
Codón sin Sentido/genética , Citoplasma/metabolismo , ARN Helicasas/metabolismo , Citoesqueleto de Actina/efectos de los fármacos , Citoesqueleto de Actina/metabolismo , Línea Celular , Citocalasina D/farmacología , Citoplasma/efectos de los fármacos , Citoesqueleto/efectos de los fármacos , Citoesqueleto/metabolismo , Depsipéptidos/farmacología , Regulación hacia Abajo/efectos de los fármacos , Regulación hacia Abajo/genética , Humanos , Degradación de ARNm Mediada por Codón sin Sentido/efectos de los fármacos , Degradación de ARNm Mediada por Codón sin Sentido/genética , Biosíntesis de Proteínas/efectos de los fármacos , Ribonucleoproteínas/metabolismo
10.
J Mol Recognit ; 32(3): e2773, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-30565321

RESUMEN

AFMBioMed is the founding name under which international conferences and summer schools are organized around the application of atomic force microscopy in life sciences and nanomedicine. From its inception at the Atomic Energy Commission in Marcoule near 2004 to its creation in 2007 and to its 10th anniversary conference in Krakow, a brief narrative history of its birth and rise will demonstrate how and what such an organization brings to laboratories and the AFM community. With the current planning of the next AFMBioMed conference in Münster in 2019, it will be 15 years of commitment to these events.


Asunto(s)
Microscopía de Fuerza Atómica , Publicaciones Periódicas como Asunto/historia , Congresos como Asunto , Historia del Siglo XX
11.
PLoS Pathog ; 13(4): e1006331, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-28430827

RESUMEN

Toxoplasma gondii possesses a highly polarized secretory system, which efficiently assembles de novo micronemes and rhoptries during parasite replication. These apical secretory organelles release their contents into host cells promoting parasite invasion and survival. Using a CreLox-based inducible knock-out strategy and the ddFKBP over-expression system, we unraveled novel functions of the clathrin adaptor complex TgAP1. First, our data indicate that AP1 in T. gondii likely functions as a conserved heterotetrameric complex composed of the four subunits γ, ß, µ1, σ1 and interacts with known regulators of clathrin-mediated vesicular budding such as the unique ENTH-domain containing protein, which we named Epsin-like protein (TgEpsL). Disruption of the µ1 subunit resulted in the mis-sorting of microneme proteins at the level of the Trans-Golgi-Network (TGN). Furthermore, we demonstrated that TgAP1 regulates rhoptry biogenesis by activating rhoptry protein exit from the TGN, but also participates in the post-Golgi maturation process of preROP compartments into apically anchored club-shaped mature organelles. For this latter activity, our data indicate a specific functional relationship between TgAP1 and the Rab5A-positive endosome-like compartment. In addition, we unraveled an original role for TgAP1 in the regulation of parasite division. APµ1-depleted parasites undergo normal daughter cell budding and basal complex assembly but fail to segregate at the end of cytokinesis.


Asunto(s)
Complejo 1 de Proteína Adaptadora/metabolismo , Proteínas Protozoarias/metabolismo , Toxoplasma/metabolismo , Complejo 1 de Proteína Adaptadora/genética , Animales , División Celular , Clatrina/genética , Clatrina/metabolismo , Citocinesis , Endosomas/metabolismo , Expresión Génica , Técnicas de Inactivación de Genes , Aparato de Golgi/metabolismo , Espectrometría de Masas , Modelos Biológicos , Orgánulos/metabolismo , Transporte de Proteínas , Proteínas Protozoarias/genética , Toxoplasma/genética , Toxoplasma/ultraestructura , Red trans-Golgi/metabolismo
12.
Semin Cell Dev Biol ; 60: 155-167, 2016 12.
Artículo en Inglés | MEDLINE | ID: mdl-27448494

RESUMEN

Bacterial pathogens display an impressive arsenal of molecular mechanisms that allow survival in diverse host niches. Subversion of plasma membrane and cytoskeletal functions are common themes associated to infection by both extracellular and intracellular pathogens. Moreover, intracellular pathogens modify the structure/stability of their membrane-bound compartments and escape degradation from phagocytic or autophagic pathways. Here, we review the manipulation of host membranes by Listeria monocytogenes, Francisella tularensis, Shigella flexneri and Yersinia spp. These four bacterial model pathogens exemplify generalized strategies as well as specific features observed during bacterial infection processes.


Asunto(s)
Membrana Celular/microbiología , Francisella/fisiología , Interacciones Huésped-Patógeno/fisiología , Listeria/fisiología , Shigella/fisiología , Yersinia/fisiología , Animales , Humanos
13.
Int J Mol Sci ; 19(6)2018 Jun 05.
Artículo en Inglés | MEDLINE | ID: mdl-29874861

RESUMEN

Heparin-binding haemagglutinin (HBHA) is a surface-exposed virulence factor of Mycobacterium tuberculosis and is involved in the binding of mycobacteria to non-phagocytic cells, allowing for extra-pulmonary dissemination of the bacilli. Despite its surface exposure, HBHA is not produced as a pre-protein containing a typical cleavable N-terminal signal peptide and is thus likely secreted by a Sec-independent, as of yet unknown mechanism. Here, we used the bacterial adenylate cyclase two-hybrid system to identify the proteins encoded by rv0613c and mmpL14 as being able to interact with HBHA. Our study was focused on Rv0613c, as it showed more consistent interactions with HBHA than MmpL14. Deletion of its orthologous gene MSMEG_1285 in recombinant Mycobacterium smegmatis producing HBHA from M. tuberculosis resulted in the loss of proper surface exposure of HBHA, as evidenced by atomic force microscopy. Furthermore, the lack of MSMEG_1285 also abolished the clumping phenotype and rough colony morphology of the recombinant M. smegmatis and reduced its adherence to A549 epithelial cells. These phenotypes have previously been associated with surface-exposed HBHA. Thus, MSMEG_1285 is directly involved in the proper cell-surface exposure of HBHA. These observations identify MSMEG_1285/Rv0613c as the first accessory protein involved in the cell surface exposure of HBHA.


Asunto(s)
Proteínas Bacterianas/genética , Proteínas de la Membrana/genética , Mycobacterium tuberculosis/genética , Tuberculosis/genética , Células A549 , Secuencia de Aminoácidos/genética , Membrana Celular/genética , Células Epiteliales/metabolismo , Humanos , Mycobacterium smegmatis/genética , Mycobacterium smegmatis/patogenicidad , Mycobacterium tuberculosis/patogenicidad , Tuberculosis/microbiología , Factores de Virulencia/genética
14.
Cytometry A ; 91(10): 983-994, 2017 10.
Artículo en Inglés | MEDLINE | ID: mdl-28544095

RESUMEN

Tuberculosis (TB) is still a major global threat, killing more than one million persons each year. With the constant increase of Mycobacterium tuberculosis strains resistant to first- and second-line drugs, there is an urgent need for the development of new drugs to control the propagation of TB. Although screenings of small molecules on axenic M. tuberculosis cultures were successful for the identification of novel putative anti-TB drugs, new drugs in the development pipeline remains scarce. Host-directed therapy may represent an alternative for drug development against TB. Indeed, M. tuberculosis has multiple specific interactions within host phagocytes, which may be targeted by small molecules. In order to enable drug discovery strategies against microbes residing within host macrophages, we developed multiple fluorescence-based HT/CS phenotypic assays monitoring the intracellular replication of M. tuberculosis as well as its intracellular trafficking. What we propose here is a population-based, multi-parametric analysis pipeline that can be used to monitor the intracellular fate of M. tuberculosis and the dynamics of cellular events such as phagosomal maturation (acidification and permeabilization), zinc poisoning system or lipid body accumulation. Such analysis allows the quantification of biological events considering the host-pathogen interplay and may thus be derived to other intracellular pathogens. © 2017 International Society for Advancement of Cytometry.


Asunto(s)
Mycobacterium tuberculosis/metabolismo , Tuberculosis/microbiología , Animales , Antituberculosos/farmacología , Bioensayo/métodos , Células Cultivadas , Descubrimiento de Drogas/métodos , Fluorescencia , Macrófagos/metabolismo , Macrófagos/microbiología , Masculino , Ratones , Ratones Endogámicos C57BL , Células RAW 264.7 , Tuberculosis/tratamiento farmacológico
15.
Microb Pathog ; 103: 129-134, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27993700

RESUMEN

Staphylococcus aureus (S. aureus) is one of several opportunistic microbial pathogens associated with many healthcare problems. In the present study, S. aureus was assessed for its biofilm-forming ability on materials routinely used in dental offices, including stainless steel (SS), polyethylene (PE), and polyvinyl chloride (PVC). Materials that were tested were characterized for roughness (Ra) and surface free energy (SFE). The adhesion forces exerted by S. aureus to each substratum were investigated using atomic force microscopy (AFM), and biofilm formation was quantitatively assessed by crystal violet staining assay. AFM measurements demonstrated that the strongest adhesion forces (20 nN) were exerted on the PE surfaces (P < 0.05) and depended more on Ra. In addition, the results of biofilm formation capability indicated that S. aureus exhibited more affinity to SS materials when compared to the other materials (P < 0.05). This ability of biofilm formation seems to be more correlated to SFE (R = 0.65). Hence, control of the surface properties of materials used in dental practices is of crucial importance for preventing biofilm formation on dental materials to be used for patients' dental care.


Asunto(s)
Adhesión Bacteriana , Infección Hospitalaria , Consultorios Odontológicos , Infecciones Oportunistas , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/fisiología , Biopelículas , Humanos , Microscopía de Fuerza Atómica , Staphylococcus aureus/ultraestructura , Propiedades de Superficie
16.
Microb Pathog ; 91: 61-7, 2016 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-26620082

RESUMEN

Orthodontic and other oral appliances act as reservoir of opportunistic pathogens that can easily become resistant to antibiotics and cause systemic infections. The aim of this study was to investigate the ability of Staphylococcus aureus strains isolated from healthy patients with orthodontic appliances, to adhere to biotic (HeLa cells) and abiotic surfaces (polystyrene and dental alloy). Adhesive ability to polystyrene was tested by crystal violet staining and quantitative biofilm production on dental alloy surfaces was evaluated by MTT reduction assay. In addition, the presence of icaA and icaD genes was achieved by polymerase chain reaction (PCR). Qualitative biofilm production revealed that 70.6% of strains were slime producers. The metabolic activity of S. aureus biofilms on dental alloy surfaces was high and did not differ between tested strains. Moreover, all the isolates were adhesive to HeLa cells and 94% of them harbor icaA and icaD genes. Considerable adhesion and internalization capacity to the epithelial HeLa cells and strong biofilm production abilities together, with a high genotypic expression of icaA/icaD genes are an important equipment of S. aureus to colonize orthodontic appliances and eventually to disseminate towards other body areas.


Asunto(s)
Adhesión Bacteriana , Contaminación de Equipos/estadística & datos numéricos , Aparatos Ortodóncicos/microbiología , Staphylococcus aureus/fisiología , Adolescente , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Biopelículas , Femenino , Humanos , Masculino , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/clasificación , Staphylococcus aureus/genética , Staphylococcus aureus/aislamiento & purificación , Adulto Joven
17.
Methods ; 75: 61-8, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25667106

RESUMEN

Autophagy is a predominant eukaryotic mechanism for the engulfment of "portions" of cytoplasm allowing their degradation to recycle metabolites. The autophagy is ubiquitous among the life kingdom revealing the importance of this pathway that appears more complex than previously thought. Several reviews have already addressed how to monitor this pathway and have highlighted the existence of new routes such as the LC3-associated phagocytosis (LAP) and the non-canonical autophagy. The principal difference between autophagosomes and LAP vacuoles is that the former has two limiting membranes positives for LC3 whereas the latter has one. Herein, we propose to emphasize the use of correlative light electron microscopy (CLEM) to answer some autophagy's related questions. The structured illumination microscopy (SIM) relatively easy to implement allows to better observe the Atg proteins recruitment and localization during the autophagy process. While LC3 recruitment is performed using light microscopy the ultrastructural morphological analysis of LC3-vacuoles is ascertained by electron microscopy. Hence, these combined and correlated approaches allow to tackle the LAP vs. autophagosome issue.


Asunto(s)
Autofagia/genética , Citoplasma/ultraestructura , Fagosomas/ultraestructura , Citoplasma/metabolismo , Microscopía Electrónica , Proteínas Asociadas a Microtúbulos/metabolismo , Fagosomas/metabolismo , Yersinia
18.
Microb Pathog ; 86: 1-9, 2015 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-26055540

RESUMEN

The oral cavity is regarded as a relevant site for Staphylococcus aureus colonization. However, characterization of virulence mechanisms of oral S. aureus remains to be uncovered. In this study, twenty one S. aureus strains isolated from the oral cavity of Tunisian patients were screened for adherence, invasion and cytotoxicity against HeLa cells. In addition, the presence of adhesins (icaA, icaD, can, fnbA and fnbB) and α-hemolysin (hla) genes in each strain was achieved by polymerase chain reaction (PCR). Our finding revealed that oral S. aureus strains were able to adhere and invade epithelial cells, with variable degrees (P < 0.05). Moreover they exhibited either low (23.8%) or moderate (76.2%) cytotoxic effects. In addition 76.2% of strains were icaA and icaD positive and 90.5% harbor both the fnbA and the fnbB gene. While the cna gene was detected in 12 strains (57.2%). Furthermore, the hla gene encoding the α-toxin was found in 52.4% of the isolates. All these virulence factors give to S. aureus the right qualities to become a redoubtable pathogen associated to oral infections.


Asunto(s)
Adhesión Bacteriana , Endocitosis , Staphylococcus aureus/fisiología , Staphylococcus aureus/patogenicidad , Adhesinas Bacterianas/genética , Toxinas Bacterianas/genética , Supervivencia Celular , Células HeLa , Proteínas Hemolisinas/genética , Humanos , Reacción en Cadena de la Polimerasa , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/citología , Staphylococcus aureus/aislamiento & purificación , Túnez , Virulencia
19.
Microb Biotechnol ; 16(3): 618-631, 2023 03.
Artículo en Inglés | MEDLINE | ID: mdl-36541039

RESUMEN

Yarrowia lipolytica is a dimorphic oleaginous non-conventional yeast widely used as a powerful host for expressing heterologous proteins, as well as a promising source of engineered cell factories for various applications. This microorganism has a documented use in Feed and Food and a GRAS (generally recognized as safe) status. Moreover, in vivo studies demonstrated a beneficial effect of this yeast on animal health. However, despite the focus on Y. lipolytica for the industrial manufacturing of heterologous proteins and for probiotic effects, its potential for oral delivery of recombinant therapeutic proteins has seldom been evaluated in mammals. As the first steps towards this aim, we engineered two Y. lipolytica strains, a dairy strain and a laboratory strain, to produce the model fluorescent protein mCherry. We demonstrated that both Y. lipolytica strains transiently persisted for at least 1 week after four daily oral administrations and they maintained the active expression of mCherry in the mouse intestine. We used confocal microscopy to image individual Y. lipolytica cells of freshly collected intestinal tissues. They were found essentially in the lumen and they were rarely in contact with epithelial cells while transiting through the ileum, caecum and colon of mice. Taken as a whole, our results have shown that fluorescent Y. lipolytica strains constitute novel tools to study the persistence and dynamics of orally administered yeasts which could be used in the future as oral delivery vectors for the secretion of active therapeutic proteins in the gut.


Asunto(s)
Yarrowia , Animales , Ratones , Yarrowia/genética , Proteínas Recombinantes/genética , Imagen Óptica , Intestinos , Ingeniería Metabólica/métodos , Mamíferos/metabolismo
20.
Nanoscale ; 15(29): 12255-12269, 2023 Jul 27.
Artículo en Inglés | MEDLINE | ID: mdl-37378568

RESUMEN

Monocytes activated by pro-inflammatory signals adhere to the vascular endothelium and migrate from the bloodstream to the tissue ultimately differentiating into macrophages. Cell mechanics and adhesion play a crucial role in macrophage functions during this inflammatory process. However, how monocytes change their adhesion and mechanical properties upon differentiation into macrophages is still not well understood. In this work, we used various tools to quantify the morphology, adhesion, and viscoelasticity of monocytes and differentiatted macrophages. Combination of atomic force microscopy (AFM) high resolution viscoelastic mapping with interference contrast microscopy (ICM) at the single-cell level revealed viscoelasticity and adhesion hallmarks during monocyte differentiation into macrophages. Quantitative holographic tomography imaging revealed a dramatic increase in cell volume and surface area during monocyte differentiation and the emergence of round and spread macrophage subpopulations. AFM viscoelastic mapping showed important stiffening (increase of the apparent Young's modulus, E0) and solidification (decrease of cell fluidity, ß) on differentiated cells that correlated with increased adhesion area. These changes were enhanced in macrophages with a spread phenotype. Remarkably, when adhesion was perturbed, differentiated macrophages remained stiffer and more solid-like than monocytes, suggesting a permanent reorganization of the cytoskeleton. We speculate that the stiffer and more solid-like microvilli and lamellipodia might help macrophages to minimize energy dissipation during mechanosensitive activities. Thus, our results revealed viscoelastic and adhesion hallmarks of monocyte differentiation that may be important for biological function.


Asunto(s)
Microscopía , Monocitos , Monocitos/metabolismo , Macrófagos/metabolismo , Módulo de Elasticidad , Diferenciación Celular , Adhesión Celular
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