RESUMEN
Long noncoding RNA (lncRNA) (GABPB1-IT1) has been reported to be downregulated in lung cancer, while its expression and function in other cancers are unknown. In this study, the expression levels of GABPB1-IT1 in tissue samples from 62 ccRCC patients were measured by performing RT-qPCR. Potential base pairing formed between GABPB1-IT1 and miR-21 was explored using the online program IntaRNA 2.0 and further confirmed by Dual-luciferase activity assay and RNA pulldown assay. The role of GABPB1-IT1 and miR-21 in regulating the expression of PTEN was evaluated by RT-qPCR and Western blot. The role of GABPB1-IT1, miR-21, and PTEN in regulating the proliferation of Caki-2 cells was explored by CCK-8 assay. It was observed that GABPB1-IT1 was downregulated in ccRCC and predicted poor survival. GABPB1-IT1 directly interacted with miR-21, while it did not regulate the expression of each other. Moreover, upregulation of PTEN, which is a target of miR-21, was observed in ccRCC cells with overexpression of GABPB1-IT1. Overexpression of GABPB1-IT1 and PTEN decreased the proliferation rates of ccRCC cells. In addition, overexpression of GABPB1-IT1 reduced the enhancing effects of miR-21 on cell proliferation. Therefore, GABPB1-IT1 may upregulate PTEN by sponging miR-21 in ccRCC to inhibit cancer cell proliferation. Our study characterized a novel GABPB1-IT1/miR-21/PTEN axis in ccRCC.
Asunto(s)
Carcinoma de Células Renales , Neoplasias Renales , MicroARNs , ARN Largo no Codificante , Humanos , Carcinoma de Células Renales/metabolismo , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , MicroARNs/genética , MicroARNs/metabolismo , Línea Celular Tumoral , Neoplasias Renales/genética , Neoplasias Renales/metabolismo , Transformación Celular Neoplásica , Carcinogénesis/genética , Proliferación Celular/genética , Regulación Neoplásica de la Expresión Génica , Fosfohidrolasa PTEN/genética , Fosfohidrolasa PTEN/metabolismo , Factor de Transcripción de la Proteína de Unión a GA/genética , Factor de Transcripción de la Proteína de Unión a GA/metabolismoRESUMEN
OBJECTIVE: To establish a predictive model of systemic inflammatory response syndrome (SIRS) after percutaneous nephrolithotomy (PCNL) for kidney calculi based on logistic regression. METHODS: The data of 148 patients with unilateral kidney calculi treated in Xi'an International Medical Center Hospital from October 2019 to September 2022 were analyzed retrospectively. According to development of SIRS after PCNL, the patients were divided into one group with SIRS after operation (occurrence group, n = 19) and another group without SIRS after operation (non-occurrence group, n = 129). The clinical data of patients were collected, and risk factors for SIRS after PCNL in patients with unilateral kidney calculi were analyzed by logistic regression. RESULTS: Gender, body mass index (BMI), hypertension, diabetes mellitus (DM), calculi size ≥ 30 mm, renal insufficiency, and hydronephrosis were risk factors for postoperative SIRS (P < 0.05). According to multivariate logistic regression analysis, BMI, DM, hypertension, calculi size ≥ 30 mm, and hydronephrosis were independent risk factors for SIRS (P < 0.05). Based on the regression coefficient, a predictive model was established. The occurrence group had a higher risk score than the non-occurrence group (P < 0.05). According to receiver operating characteristic (ROC) curve-based analysis, the area under the curve of risk score for predicting SIRS in patients was 0.898. CONCLUSION: Patients with BMI ≥ 25 kg/m2, DM, hypertension, calculi ≥ 30 mm, and/or hydronephrosis are more likely to suffer SIRS after PCNL. The risk score has high clinical value in the prediction of SIRS.