RESUMEN
Gestational diabetes mellitus (GDM) has become a worldwide problem in recent years. Macrosomia, a primary consequence of GDM, has short-term and life-long consequences in the offspring of mothers with GDM. Our previous study showed that miR-517a was dysregulated in placenta and plasma of fetal growth restriction through inhibiting invasion of trophoblast and might be closely related with the regulation of birth weight by the placenta. To further investigate the mechanism of miR-517a, we conducted genome-wide microarray profile of lncRNAs. lncRNA-SNX17 was found to be significantly upregulated in the placenta of diabetic macrosomia by qRT-PCR, and the expression of miR-517a and IGF-1 were measured by qRT-PCR and Western blot. Interestingly, significant inverse correlations of the miR-517a with both lncRNA-SNX17 and IGF-1 expression were revealed in the placenta of diabetic macrosomia. Bioinformatic prediction also revealed that both lncRNA-SNX17 and IGF-1 possessed binding sites for miR-517a, which were then confirmed by luciferase report assay. LncRNA-SNX17 overexpression reduced the expression of miR-517a and increased the IGF-1 expression in HTR-8/SVneo human trophoblast cell line and thus enhanced the proliferation of HTR-8/SVneo. The enhancement of HTR-8/SVneo proliferation by lncRNA-SXN17 could be nullified by co-transfection of miR-517a mimics. The data suggested that lncRNA-SNX17 might promote the trophoblast proliferation through miR-517a/IGF-1 pathway and might play a role in the placentation of diabetic macrosomia.
Asunto(s)
Diabetes Gestacional/metabolismo , Macrosomía Fetal/etiología , Macrosomía Fetal/metabolismo , Factor I del Crecimiento Similar a la Insulina/metabolismo , MicroARNs/metabolismo , Placenta/metabolismo , Embarazo en Diabéticas/metabolismo , ARN Largo no Codificante/metabolismo , Adulto , Western Blotting , Línea Celular , Femenino , Humanos , Análisis de Secuencia por Matrices de Oligonucleótidos , Embarazo , Reacción en Cadena en Tiempo Real de la Polimerasa , Trofoblastos/metabolismoRESUMEN
A novel and facile approach to produce TiO2-based superhydrophobic-superhydrophilic patterns by UV or solar irradiation without a photomask is presented. The fabricated superhydrophobic-superhydrophilic patterns with excellent mechanical properties have long lifetimes in outdoor applications or other UV environments because of a self-supply of low surface tension material.