From pollution to solutions: Insights into the sources, transport and management of plastic debris in pristine and urban rivers.

River systems are important recipients of environmental plastic pollution and have become key pathways for the transfer of mismanaged waste from the land to the ocean. Understanding the sources and fate of plastic debris, including plastic litter (>5 mm) and microplastics (MPs) (<5 mm), entering different riverine systems is essential to mitigate the ongoing environmental plastic pollution crisis. We comprehensively investigated the plastic pollution in the catchments of two rivers in the Yangtze River basin: an urban river, the Suzhou section of the Beijing-Hangzhou Grand Canal (SZ); and a pristine rural river, the Jingmen section of the Hanjiang River (JM). The abundance of plastic pollutants in SZ was significantly higher than in JM: 0.430 ± 0.450 items/m3 and 0.003 ± 0.003 items/m3 of plastic litter in the water; 23.47 ± 25.53 n/m3 and 2.78 ± 1.55 n/m3 MPs in the water; and 218.82 ± 77.40 items/kg and 5.30 ± 1.99 items/kg of MPs in the sediment, respectively. Plastic litter and MPs were closely correlated in abundance and polymer composition. Overall, the polymer type, shape and color of MPs were dominant by polypropylene (42.5%), fragment (60.4%) and transparent (40.0%), respectively. Source tracing analysis revealed that packaging, shipping, and wastewater were the primary sources of plastic pollutants. The mantel analysis indicated that socio-economic and geospatial factors play crucial roles in driving the hotspot formation of plastic pollution in river networks. The composition of the MP communities differed significantly between the sediments and the overlying water. The urban riverbed sediments had a more pronounced pollutant 'sink' effect compared with the pristine rivers. These findings suggested that the modification of natural streams during urbanization may influence the transport and fate of plastic pollutants in them. Our results offer pivotal insights into effective preventive measures.

Controlling Factors of Microplastic Riverine Flux and Implications for Reliable Monitoring Strategy.

A significant proportion of marine plastic debris and microplastics is assumed to be derived from river systems. In order to effectively manage plastic contamination of the marine environment, an accurate quantification of riverine flux of land-based plastics and microplastics is imperative. Rivers not only represent pathways to the ocean, but are also complex ecosystems that support many life processes and ecosystem services. Yet riverine microplastics research is still in its infancy, and many uncertainties still remain. Major barriers exist in two aspects. First, nonharmonized sampling methodologies make it problematic for compiling data across studies to better estimate riverine fluxes of microplastics globally; Second, the significant spatiotemporal variation of microplastics in rivers which was affected by the river characteristics, MPs properties, etc. also have important influence on the estimation of riverine MPs fluxes. In this study, we made a comprehensive review from the above two aspects based on published peer-reviewed studies and provide recommendations and suggestions for a reliable monitoring strategy of riverine MPs, which is beneficial to the further establish sampling methods for rivers in different geographical locations. Besides, methods for achieving a high level of comparability across studies in different geographical contexts are highlighted. Riverine microplastic flux monitoring is another important part of this manuscript. The influential factors and calculation methods of microplastic flux in rivers are also discussed in this paper.

Unpalatable Plastic: Efficient Taste Discrimination of Microplastics in Planktonic Copepods.

Planktonic copepods are the most abundant animals in the ocean and key players in global biochemical processes. Recent modeling suggests that zooplankton ingestion of microplastics (MPs) can disrupt the biological carbon pump and accelerate a global loss of oceanic oxygen. Here we investigate the behavioral responses and ingestion rates of a model feeding-current generating copepod when exposed to microplastics of different characteristics by small-scale video observations and bottle incubations. We found that copepods rejected 80% of the microplastics after touching them with their mouth parts, in essence exhibiting a kind of taste discrimination. High rejection rates of microplastics were independent of polymer type, shape, presence of biofilms, or sorbed pollutant (pyrene), indicating that microplastics are unpalatable for feeding-current feeding copepods and that post-capture taste discrimination is a main sensorial mechanism in the rejection of microplastics. In an ecological context, taking into account the behaviors of planktonic copepods and the concentrations of microplastics found in marine waters, our results suggest a low risk of microplastic ingestion by zooplankton and a low impact of microplastics on the vertical exportation of fecal pellets.

Profiling the Vertical Transport of Microplastics in the West Pacific Ocean and the East Indian Ocean with a Novel in Situ Filtration Technique.

A new technique involving large-volume (10 m3) samples of seawater was used to determine the abundance of microplastics (MPs) in the water column in the West Pacific Ocean and the East Indian Ocean. Compared to the conventional sampling methods based on smaller volumes of water, the new data yielded abundance values for the deep-water column that were at least 1-2 orders of magnitude lower. The data suggested that limited bulk volumes currently used for surface sampling are insufficient to obtain accurate estimates of MP abundance in deep water. Size distribution data indicated that the lateral movement of MPs into the water column contributed to their movement from the surface to the bottom. This study provides a reliable dataset for the water column to enable a better understanding of the transport and fate of plastic contamination in the deep-ocean ecosystem.

An Efficient and Lightweight Convolutional Neural Network for Remote Sensing Image Scene Classification.

Classifying remote sensing images is vital for interpreting image content. Presently, remote sensing image scene classification methods using convolutional neural networks have drawbacks, including excessive parameters and heavy calculation costs. More efficient and lightweight CNNs have fewer parameters and calculations, but their classification performance is generally weaker. We propose a more efficient and lightweight convolutional neural network method to improve classification accuracy with a small training dataset. Inspired by fine-grained visual recognition, this study introduces a bilinear convolutional neural network model for scene classification. First, the lightweight convolutional neural network, MobileNetv2, is used to extract deep and abstract image features. Each feature is then transformed into two features with two different convolutional layers. The transformed features are subjected to Hadamard product operation to obtain an enhanced bilinear feature. Finally, the bilinear feature after pooling and normalization is used for classification. Experiments are performed on three widely used datasets: UC Merced, AID, and NWPU-RESISC45. Compared with other state-of-art methods, the proposed method has fewer parameters and calculations, while achieving higher accuracy. By including feature fusion with bilinear pooling, performance and accuracy for remote scene classification can greatly improve. This could be applied to any remote sensing image classification task.

Consistent Transport of Terrestrial Microplastics to the Ocean through Atmosphere.

Although atmospheric transport and deposition could be an important pathway of terrestrial pollutants to the ocean, little information concerning the presence and distribution of these suspended atmospheric microplastics in marine air is available. We investigated, for the first time, the occurrence and distribution of suspended atmospheric microplastics (SAMPs) in the west Pacific Ocean. In this study, the spatial distribution, morphological appearance, and chemical composition of suspended atmospheric microplastics were studied through continuous sampling during a cruise. SAMPs abundance ranged from 0 to 1.37 n/m3, the median of 0.01 n/m3. Fiber, fragment, and granule SAMPs quantitively constituted 60%, 31%, and 8% of all MPs, respectively. Interestingly, plastic microbeads with numerical proportion of 5% were also observed. A high suspended atmospheric microplastics abundance was found in the coastal area (0.13 ± 0.24 n/m3), while there was less amount detected in the pelagic area (0.01 ± 0.01 n/m3). The amount of suspended atmospheric microplastics collected during the daytime (0.45 ± 0.46 n/m3) was twice the amount collected at night (0.22 ± 0.19 n/m3), on average. Our observations provide field-based evidence that suspended atmospheric microplastics are an important source of microplastics pollution in the ocean, especially the pollution caused by textile microfibers.

The chemokinome superfamily in channel catfish: I. CXC subfamily and their involvement in disease defense and hypoxia responses.

Chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. They are defined by the presence of four conserved cysteine residues and are divided into four subfamilies depending on the arrangement of the first two conserved cysteines residues: CXC, CC, C and CX3C. In this study, a complete set of 17 CXC chemokine ligand (CXCL) genes was systematically identified and characterized from channel catfish genome through data mining of existing genomic resources. Phylogenetic analysis allowed annotation of the 17 CXC chemokines. Extensive comparative genomic analyses supported their annotations and orthologies, revealing the existence of fish-specific CXC chemokines and the expansion of CXC chemokines in the teleost genomes. The analysis of gene expression after bacterial infection indicated the CXC chemokines were expressed in a gene-specific manner. CXCL11.3 and CXCL20.3 were expressed significantly higher in resistant fish than in susceptible fish after ESC infection, while CXCL20.2 were expressed significantly higher in resistant fish than in susceptible fish after columnaris infection. The expression of those CXC chemokines, therefore can be a useful indicator of disease resistance. A similar pattern of expression was observed between resistant and susceptible fish with biotic and abiotic stresses, ESC, columnaris and hypoxia, suggesting that high levels of expression of the majority of CXC chemokines, with exception of CXC11 and CXC20, are detrimental to the host.

Septin genes in channel catfish (Ictalurus punctatus) and their involvement in disease defense responses.

Septins are an evolutionarily conserved family of GTP-binding proteins. They are involved in diverse processes including cytokinesis, apoptosis, infection, neurodegeneration and neoplasia. In this study, through thorough data mining of existed channel catfish genomic resources, we identified a complete set of 15 septin genes. Septins were classified into four subgroups according to phylogenetic analysis. Extensive comparative genomic analysis, including domain and syntenic analysis, supported their annotation and orthologies. The expression patterns of septins in channel catfish were examined in healthy tissues and after infection with two major bacterial pathogens, Edwardsiella ictaluri and Flavobacterium columnare. In healthy channel catfish, most septin genes were ubiquitously expressed and presented diversity patterns in various tissues, especially mucosal tissues, proposing the significant roles septin genes may play in maintaining homeostasis and host immune response activities. After bacterial infections, most septin genes were regulated, but opposite direction in expression profiles were found with the two bacterial pathogens: the differentially expressed septin genes were down-regulated in the intestine after E. ictaluri infection while generally up-regulated in the gill after F. columnare infection, suggesting a pathogen-specific and tissue-specific pattern of regulation. Taken together, these results suggested that septin genes may play complex and important roles in the host immune responses to bacterial pathogens in channel catfish.

A representative retinoid X receptor antagonist UVI3003 induced teratogenesis in zebrafish embryos.

Retinoid X receptor (RXR) interfering activity has been detected in different water resources. To study RXR disruptor-induced toxicological effects on vertebrates, embryos of zebrafish (Danio rerio) were exposed to a representative RXR antagonist UVI3003. Results showed that the teratogenic index (LC50 /EC50 ) of UVI3003 was as high as 5.4. UVI3003 induced multiple malformations of embryos, including deformed fins, reduced brains, small jaws, bent tails and edema in hearts, the degree of which became more severe with increasing exposure concentration. Although no significant difference was observed in the hatching rates between the exposure group and control, the whole body length was significantly reduced by 6.5% and 8.9% when exposed to 200 and 300 µg l(-1) of UVI3003, respectively. The heart rate also significantly decreased by 8.8-50.2% during exposure. Further experiments revealed that the pharyngula stage was the most sensitive development phase in terms of embryo response to UVI3003. The results demonstrated severe teratogenicity of RXR antagonist in zebrafish embryos and provided important data for ecotoxicological evaluation of RXR antagonists.

Quantitative toxicoproteomic analysis of zebrafish embryos exposed to a retinoid X receptor antagonist UVI3003.

Retinoid X receptor (RXR) antagonists, including some environmental endocrine disruptors, have a teratogenic effect on vertebrate embryos. To investigate the toxicological mechanism on the protein expression level, a quantitative proteomic study was conducted to analyze the proteome alterations of zebrafish (Danio rerio) embryos exposed to gradient concentrations of a representative RXR antagonist UVI3003. Using isobaric Tags for Relative and Absolute Quantitation (iTRAQ) labeling coupled nano high-performance liquid chromatography-tandem mass spectrometry (nano HPLC-MS/MS), in total 6592 proteins were identified, among which 195 proteins were found to be differentially expressed by more than a two-fold change in exposed groups compared with the control. Gene ontology analysis showed that these differential proteins were mostly involved in anatomical structure development, biosynthetic process, ion binding and oxidoreductase activity. Moreover, the biological pathways of translation, lipoprotein metabolism, cell survival and gluconeogenesis were intensively inhibited after exposure. Some significantly downregulated proteins such as apolipoprotein A-I and vitellogenin and upregulated proteins such as calcium activated nucleotidase 1b, glutathione S-transferase and glucose 6-dehydrogenases showed a strong dose-dependent response. The results provided new insight into the molecular details of RXR antagonist-induced teratogenicity and added novel information of pathways and potential biomarkers for evaluation of RXR interfering activity.

Combined toxic effects of nanoplastics and norfloxacin on antioxidant and immune genes in mussels.

Nanoplastics (NPs) and antibiotics (ABs) are two of the emerging marine contaminants that have drawn the most attention in recent years. Given the necessity of figuring out the effects of plastic and antibiotic contamination on marine organism life and population in the natural environment, it is essential to apply rapid and effective biological indicators to evaluate their comprehensive toxic effects. In this study, using mussel (Mytilus coruscus) as a model, we investigated the combined toxic effects of NP (80 nm polystyrene beads) and AB (Norfloxacin, NOR) at environmental-relevant concentrations on antioxidant and immune genes. In terms of the antioxidant genes, NPs significantly increased the relative expression of Cytochrome P450 3A-1 (CYP3A-1) under various concentrations of NOR conditions, but they only significantly increased the relative expression of CYP3A-2 in the high concentration (500 µg L-1 NOR) co-exposure group. In the NP-exposure group which exposed to no or low concentrations of NOR, nuclear factor erythroid 2-related factor 2 (Nrf2) was upregulated. In terms of the immune genes, interleukin-1 receptor-associated kinase (IRAK) -1 showed a significant increase in the low-concentration NOR group while a significant inhibition in the high-concentration NOR group. Due to the presence of NPs, exposure to NOR resulted in a significant increase in both IRAK-4 and heat shock protein (HSP) 70. Our findings indicate that polystyrene NPs can exacerbate the effects of NOR on the anti-oxidant and immune defense performance of mussels. This study delves into the toxic effects of NPs and ABs from a molecular perspective. Given the expected increase in environmental pollution due to NPs and ABs, future research is needed to investigate the potential synergistic effect of NPs and ABs on other organisms.

Ingestion and adherence of microplastics by estuarine mysid shrimp.

Microplastics have been reported to be present in zooplankton, yet questions persist regarding their fate and dynamics within biota. We selected the commercial mysid shrimp, Mesopodopsis orientalis, as the focal zooplankton for this study due to their crucial role in our study area, the Inner Gulf of Thailand in January 2022. We investigated the presence of microplastics in mysid bodies and fecal pellets, examining both attached microplastics on external body parts and those ingested. In addition, we conducted microplastic feeding experiments, exposing mysids to various treatments of microplastics. The results of the field investigation indicate that mysids exhibited an average of 0.12 ± 0.03 microplastic items/mysid from whole-body samples. The shape, type, and color of microplastics found in mysids were similar to those present in seawater, with blue PET microfibers being the most prevalent. Our observations on live mysids revealed that microplastics were acquired through ingestion and adherence to appendages and exoskeletons. Microplastics were observed in mysid's fecal pellets at 0.09 ± 0.03 items/mysid, while microplastics adhering to the mysid's body and appendages were observed at 0.10 ± 0.04 items/mysid. The sizes of microplastics extracted from preserved mysids ranged from 58 µm to 4669 µm, with median of 507 µm. The laboratory experiments revealed that the presence of microalgae enhanced microplastic ingestion in mysids; microplastics incubated with a cyanobacterium, Oscillatoria sp., and diatom Navicula sp. significantly increased the number of microplastic particles ingested by mysids. This study showed that microplastics can be more ingested in mysids, especially when food items are present. Microplastic fate in these animals may involve expulsion into the environment or adherence, potentially facilitating their transfer up the marine food web.

Vertical distribution of microplastics in the Western Pacific Warm Pool: In situ results comparison of different sampling method.

Marine microplastics (MPs) are recognized as a growing severe environmental concern. The vertical distribution pattern of MPs in the ocean is still elusive. Meanwhile, different sampling methods have been deployed in previous studies, resulting in difficulties in compiling data. In this study, for the first time, we explored ocean interior MP pollution in the Western Pacific Warm Pool simultaneously using both a CTD (Conductivity-temperature-depth) sampler and a large-volume in-situ filtration system. At the same sampling station, the average abundance of microplastics in the water column obtained by the two sampling methods was 0.37 ± 0.44 n/m3 (in-situ filtration) and 115.12 ± 64.13 n/m3 (CTD), respectively, which showed significant differences. Both methods found that the main chemical composition and shape of MPs were PET and fiber. Ocean current was identified as the dominant factor that impacted the horizontal distribution of MPs in the study area. The abundance of MPs in the surface layer was 5.4-703.8 times higher than that of the water column. The similar physical and chemical properties of MPs in the surface water and water column indicated that MPs in the water column originate from the sustained release from the surface layer.

The combined effects of phenanthrene and micro-/nanoplastics mixtures on the cellular stress responses of the thick-shell mussel Mytilus coruscus.

Pollution with complex mixtures of contaminants including micro- and nano-plastics (MNPs) and organic pollutants like polycyclic aromatic hydrocarbons (PAH) poses a major threat to coastal marine ecosystems. Toxic mechanisms of contaminant mixtures are not well understood in marine organisms. We studied the effects of single and combined exposures to polycyclic aromatic hydrocarbon phenanthrene (Phe) and MNPs mixture with sizes of 70 nm, 5 µm and 100 µm on the immune health and oxidative stress parameters in the thick-shell mussel Mytilus coruscus. Immune cells (hemocytes) were more sensitive to the pollutant-induced oxidative stress than the gills. In hemocytes of co-exposed mussels, elevated mortality, lower lysosomal content, high production of reactive oxygen species (ROS) and decrease mitochondrial were found. Disparate responses of antioxidant enzymes in the hemolymph (e.g. increased superoxide dismutase (SOD) activity without a corresponding increase in catalase (CAT) in Phe exposures and an increase in CAT without a change in SOD in MNPs exposures) suggests misbalance of the antioxidant defense in the pollutant-exposed mussels. Gill lacked pronounced oxidative stress response showing a decline in ROS and antioxidant levels. Tissue-specific single and combined effects of Phe and MNPs suggest variation in bioavailability and/or different sensitivity to these pollutants in the studied tissues. Notably, the combined effects of MNPs and Phe were additive or antagonistic, showing that MNPs do not enhance and occasionally mitigate the toxic effects of Phe on the hemocytes and the gills of the mussels. Overall, our study sheds light on the impact of long-term exposure to MNPs and Phe mixtures on mussels, showing high sensitivity of the immune system and modulation of the Phe toxicity by MNPs co-exposure. These findings that may have implications for understanding the impacts of combined PAH and MNPs pollution on the health of mussel populations from polluted coastal habitats.

Polyamide microplastics can mitigate the effects of pathogenic bacterium on the health of marine mussels.

Vibrio parahaemolyticus and microplastics are prevalent in the ocean. Bacteria attach onto plastic particles, forming harmful biofilms that collectively threaten bivalve health. This study investigates the interaction between polyamide microplastics (PA: particle size 38 ± 12 µm) and V. parahaemolyticus, as well as their combined impact on thick-shelled mussels (Mytilus coruscus). We introduced 1011 CFU/L of V. parahaemolyticus into varying PA concentrations (0, 5, 50, and 500 particles/L) to observe growth over 14 h and biofilm formation after 48 h. Our findings indicate that microplastics suppress biofilm formation and virulence gene expression. Four treatments were established to monitor mussel responses: a control group without PA or V. parahaemolyticus; a group with 50 particles/L PA; a group with 1011 CFU/L V. parahaemolyticus; and a co-exposure group with both 50 particles/L PA and 1011 CFU/L V. parahaemolyticus, over a 14-day experiment. However, combined stress from microplastics and Vibrio led to immune dysregulation in mussels, resulting in intestinal damage and microbiome disruption. Notably, V. parahaemolyticus had a more severe impact on mussels than microplastics alone, yet their coexistence reduced some harmful effects. This study is the first to explore the interaction between microplastics and V. parahaemolyticus, providing important insights for ecological risk assessments.

Microplastics in the seagrass ecosystems: A critical review.

Marine microplastic (MP) pollution represents a global environmental issue that has ignited considerable apprehension within the international community. Seagrass beds, which serve as nearshore marine ecosystems, have emerged as focal points of plastic and MP contamination due to the pronounced density of anthropogenic activities and the hydrological mitigating effects of submerged vegetation. Nevertheless, our comprehension of MPs within seagrass ecosystems remains constrained. In this study, we employed bibliometric analyses and comprehensive data exploration to summarize the historical progression of the development, pivotal areas of interest, and research deficiencies, followed by proposing future research directions for MP pollution in seagrass beds. The 37 selected papers were sourced from the Web of Science Core Collection scientific database as of December 31st, 2022. Based on the current evaluation, MPs are ubiquitously discovered within seagrass canopies, sediments, and marine organisms, while less than 15 % of seagrass species worldwide have been investigated. Moreover, methodological inconsistencies in sampling, processing and visualization between studies hindered the fusion and comparison of data. MPs in upper sediments and seagrass blades were the most widely investigated, with an average abundance of 263.4 ± 309.2 n/kg and 0.09 ± 0.03 n/blade. In all environmental compartments, the prevalent forms of MPs comprise fibrous and fragmented particles, encompassing the dominant polymers such as polypropylene, polyethylene and polyethylene terephthalate. However, the source of MPs in seagrass beds based on MP characteristics and local hydrodynamics has not been comprehensively analyzed in previous studies. The evidence for MPs acting as pollutants and contaminant carries impacting the growth and decline of seagrass is also weak. Currently, the precise implications of MPs on submerged vegetation, organisms, and the broader seagrass ecosystem remain inconclusive. However, considering the persistent accumulation of MPs, it is imperative to explore the ecological hazards they may pose within the foreseeable future.

Degradation of plastic wastes to commercial chemicals and monomers under visible light.

Plastics are playing an incrementally extensive and irreplaceable role in human life, but with alarming cyclic unsustainability. Numerous attempts have been undertaken to recycle plastics, among which chemical recycling from waste plastics back to chemicals and monomers has attracted great attention. Herein, the depolymerization of nine types of plastics to commercial chemicals and monomers was achieved under ambient conditions via synergetic integrated uranyl-photocatalysis, which contains a process for converting five kinds of mixed plastics into a value-added product. The degradation processes were depicted in terms of variation in scanning electron microscopy imaging, distinction in the X-ray diffraction pattern, alteration in water contact angle, and dynamic in molecular weight distribution. Single electron transfer, hydrogen atom transfer, and oxygen atom transfer were synergistically involved in uranyl-photocatalysis, which were substantiated by mechanistic studies. Relying on flow system design, the chemical recycling of plastics was feasible for kilogram-scale degradation of post-consumer-waste polyethylene terephthalate bottles to commercial chemicals, displaying a promising practical application potential in the future.

Monthly variation and transport of microplastics from the Soan River into the Indus River.

The presence of plastic and microplastic pollution in freshwater systems receives extensive concerns for its accumulative trend and potential ecological impacts. This is the first annual study that investigated the monthly profile of plastic pollution in the mouth of the Soan River. Plastic pollutants comprising microplastic content up to 91.7 % were abundantly found during different seasons around the year, ranging from 132.7 items/m3 to 641.3 items/m3. The average abundance of plastics was significantly higher in August (641.3 ± 23.7 items/m3) than in other months. Overall, fibers, large microplastics (L-MPs), and transparent items were dominant by shape (57.7 %), size (61.9 %), and color (24.6 %), respectively. The highest average number of fibers (374.3/m3) and L-MPs (396 items/m3) were recorded during May and remained higher in the surface water from December to May. Fragments (432.3/m3) and S-MPs were observed higher (362.3 items/m3) during the peak rainy month of the summer monsoon season (August). Variations in the abundance and morphotypes were seemingly not only influenced by the seasonal change but also might be due to hydromorphological characteristics of the river, especially riverbed morphology, and the flow of the water. Only 5.2 % of the total items found were identified using µ-FTIR (micro-Fourier Transformed Infrared Spectroscope) which consisted of 70.7 % plastic items. Spectroscopy revealed that polyethylene terephthalate was an abundantly found polymer that largely prevailed in the form of fibers, followed by polypropylene and polyethylene. Most of the fragments, foams, and films were composed of polypropylene, polystyrene, and rayon respectively. Being an urban river, the polymeric profile demonstrated that anthropogenic activities had a significant impact on polluting the river. These findings are a very important source to understand the profile of plastic pollution in the Soan River and also a significant reference for policy-making in controlling plastic pollution among the riverine networks.

Is there a significant difference in microbiota between water and microplastic surfaces in winter? The possibility of spreading offshore into the ocean.

Environmental problems caused by microplastics (MPs) are attracting global attention. The ecological risks of bacteria attached to MPs have not been studied in detail under low temperature conditions. Here, MPs in surface water were sampled in winter from the Changjiang (or Yangtze) River Estuary. The physical and chemical characteristics of the MPs were identified, and the diversity and species composition of bacteria on the surface water MPs were analyzed. Phenotypic prediction analysis was used to analyze the potential risk of bacteria in the biofilm on the surfaces of MPs. The main chemical composition in the MPs in the surface water were PP (polypropylene), PE (polyethylene), PS (polystyrene) and other light weight MPs. Sampling sites played a decisive role in the bacterial species composition. The potential plastic-degrading bacterium Acinetobacter and the potential pathogenic bacterium Pseudomonas showed significant differences across different sampling sites. Microbial communities on the surfaces of MPs in winter were not significantly different from planktonic bacteria in the water body. Phenotypic prediction results showed that bacteria on the surface of MPs had a marked capacity to form biofilms, but a low pathogenicity risk. Based on the results of biodiversity analysis and phenotypic prediction, the potential ecological risk of bacteria in biofilms on MP surfaces is lower at low temperatures. In addition, the numerical simulation results show that the possibility of bacteria attached to MPs from the Changjiang River entering the Pacific Ocean in winter is small. MPs attached bacteria in the Changjiang estuary have low ecological risk to the estuary and the Pacific Ocean in winter.