Nonlinear 3D Ligand-Based Metal-Organic Framework for Thermodynamic-Kinetic Synergistic Splitting of Mono-/Dibranched Hexane Isomers.

The selective splitting of hexane isomers without the use of energy-intensive phase-change processes is essential for the low-carbon production of clean fuels and also very challenging. Here, we demonstrate a strategy to achieve a complete splitting of the high-RON dibranched isomer from the monobranched and linear isomers, by using a nonlinear 3D ligand to form pillar-layered MOFs with delicate pore architecture and chemistry. Compared with its isoreticular MOFs with the same ted pillar but different linear 3D or linear 2D in-layer ligands, the new MOF constructed in this work, Cu(bhdc)(ted)0.5 (ZUL-C5), exhibited an interesting "channel switch" effect which creates pore space with reduced window size and channel dimensionality together with unevenly distributed alkyl-rich adsorption sites, contributing to a greatly enhanced ability to discriminate between mono- and dibranched isomers. Evidenced by a series of studies including adsorption equilibrium/kinetics/breakthrough tests, guest-loaded single-crystal/powder XRD measurement, and DFT-D modeling, a thermodynamic-kinetic synergistic mechanism in the separation was proposed, resulting in a record production time for high-purity 2,2-dimethylbutane along with a high yield.

Clarifying the mechanism of apigenin against blood-brain barrier disruption in ischemic stroke using systems pharmacology.

Currently, recombinant tissue plasminogen activator (rtPA) is an effective therapy for ischemic stroke (IS). However, blood-brain barrier (BBB) disruption is a serious side effect of rtPA therapy and may lead to patients' death. The natural polyphenol apigenin has a good therapeutic effect on IS. Apigenin has potential BBB protection, but the mechanism by which it protects the BBB integrity is not clear. In this study, we used network pharmacology, bioinformatics, molecular docking and molecular dynamics simulation to reveal the mechanisms by which apigenin protects the BBB. Among the 146 targets of apigenin for the treatment of IS, 20 proteins were identified as core targets (e.g., MMP-9, TLR4, STAT3). Apigenin protects BBB integrity by inhibiting the activity of MMPs through anti-inflammation and anti-oxidative stress. These mechanisms included JAK/STAT, the toll-like receptor signaling pathway, and Nitrogen metabolism signaling pathways. The findings of this study contribute to a more comprehensive understanding of the mechanism of apigenin in the treatment of BBB disruption and provide ideas for the development of drugs to treat IS.

Microfluidic Chip with Fiber-Tip Sensors for Synchronously Monitoring Concentration and Temperature of Glucose Solutions.

Monitoring the properties of fluids in microfluidic chips often requires complex open-space optics technology and expensive equipment. In this work, we introduce dual-parameter optical sensors with fiber tips into the microfluidic chip. Multiple sensors were distributed in each channel of the chip, which enabled the real-time monitoring of the concentration and temperature of the microfluidics. The temperature sensitivity and glucose concentration sensitivity could reach 314 pm/°C and -0.678 dB/(g/L), respectively. The hemispherical probe hardly affected the microfluidic flow field. The integrated technology combined the optical fiber sensor with the microfluidic chip and was low cost with high performance. Therefore, we believe that the proposed microfluidic chip integrated with the optical sensor is beneficial for drug discovery, pathological research and material science investigation. The integrated technology has great application potential for micro total analysis systems (µ-TAS).

In vivo confocal microscopy of sub-basal corneal nerves and corneal densitometry after three kinds of refractive procedures for high myopia.

PURPOSE: To observe corneal nerve fibers and densitometry after small incision lenticule extraction (SMILE), femtosecond laser-assisted laser in situ keratomileusis (FS-LASIK) and laser-assisted subepithelial keratomileusis (LASEK) for high myopia. METHODS: This is a prospective, cross-sectional research study. Patients with high myopia (equivalent spherical lens: -6.00 and -11.00D) who underwent laser corneal refractive surgery were divided into three groups: SMILE, FS-LASIK and LASEK. Scheimpflug imaging of corneal nerves in five areas was observed by confocal microscopy before and 6, 12 months after surgery. Corneal densitometry was measured by Pentacam anterior segment analysis system. RESULTS: Overall, 59 patients were enrolled. The nerve density in the central area did not recover to the preoperative level in three groups until 12 months. The density and length of corneal nerves in central and lower area were better in the SMILE group 6 months postoperatively (p = 0.01), while nerve density did not differ significantly among three groups 12 months postoperatively (p = 0.18). Nerve fibers in central and temporal region were wider in LASEK than that in other two groups at 6- and 12-month follow-up. Corneal densitometry in the central 6 mm diameter was significantly higher in the LASEK group compared with other two groups 6 months postoperatively (p = 0.04). Twelve months postoperatively, corneal densitometry in range of all zone was lower in SMILE than in FS-LASIK and LASEK (p = 0.01, 0.03, 0.04). CONCLUSIONS: Compared with FS-LASIK and LASEK, SMILE-treated eyes with high myopia had certain advantages in nerve density, length and nerve connection way and had better corneal transparency after operation.

Dual-parameter demodulated torsion sensor based on the Lyot filter with a twisted polarization-maintaining fiber.

We proposed a novel torsion sensor based on the Lyot filter with the twisted polarization-maintaining fiber (PMF) acting as the birefringence medium. Lyot filter is formed by two linear polarizers and a piece of PMF. Based on the high birefringence of the PMF, the output polarization rotates with a rate equal to the twisting rate applied on the PMF, and the sensor realizes a high sensitivity of 90.072 dB/rad. The proposed sensor also demonstrated a low strain sensitivity of 2.32 ×10 - 6 rad/µÉ›. On the other hand, based on the phase hits of the polarization interference, the wavelength sensitivity reaches 15.477 nm/rad. The monitoring range of the wavelength demodulation is complementary with the intensity demodulation in one cycle, making the valid sensing range of the proposed sensor expand. The proposed highly sensitive compact torsion sensor, with large sensing range and low crosstalk, has potential applications in many fields such as manufacturing industry, civil engineering, aerospace industry and modern smart structure monitoring.

Bovine serum albumin label-free concentration sensor based on silica corrosion quantitative monitoring system.

Bovine serum albumin (BSA) label-free concentration sensor based on silica corrosion quantitative monitoring system (SCQMS) has been proposed. Anti-resonance of hollow cylindrical waveguide (HCW) in SCQMS is simulated and investigated for monitoring corrosion rate quantitatively. Hydrofluoric acid (HF) samples with different concentrations are studied respectively, and the corrosion rate is obtained by demodulating the corresponding anti-resonance dips shift and free spectral range (FSR). Therefore, a high-precision SQCMS was prepared successfully. On this basis, a highly sensitive concentration sensor based on hole-assisted dual-core fiber (HADF) is prepared. The BSA samples with concentration from 0.2 mg/mL to 0.7 mg/mL are detected. The sensor has a high sensitivity of 30.04 nm/(mg/mL) and ultra-low limit of detection (LOD) of 0.05 mg/mL for the assisted core exposed to the target solution directly. We have demonstrated the SCQMS that can be a feasible tool for precise and quantitative corrosion of silicon structure safely. In addition, the concentration sensor structure has a wide application for ultra-low LOD, simple preparation process and high integration.

High-temperature-sensitive and spectrum-contrast-enhanced sensor using a bullet-shaped fiber cavity filled with PDMS.

Low temperature sensitivity and low spectral contrast are serious but common issues for most Fabry Perot (FP) sensors with an air cavity. In this paper, a high-temperature-sensitive and spectrum-contrast-enhanced Fabry Perot interferometer (FPI) is proposed and experimentally demonstrated. The device is composed of a hollow cylindrical waveguide (HCW) filled with polydimethylsiloxane (PDMS) and a semi-elliptic PDMS end face. The semi-elliptic PDMS end face increases the spectral contrast significantly due to the focusing effect. Experimentally, the spectral contrast is 11.97 dB, which is two times higher than the sensor without semi-elliptic PDMS end face. Ultra-high temperature sensitivity of 3.1501 nm/°C was demonstrated. The proposed sensor exhibits excellent structural stability, high spectral contrast and high temperature sensitivity, showing great potential in biomedicine, industrial manufacturing, agricultural production and other applications.

Secondary organic aerosol formation and aging from ambient air in an oxidation flow reactor during wintertime in Beijing, China.

Secondary organic aerosols (SOA) constitute a large fraction of atmospheric aerosols, yet our knowledge of the formation and aging processes of SOA in megacities of China is still limited. In this work, the formation and aging processes of SOA in winter in Beijing was investigated using a high-resolution aerosol mass spectrometer (AMS) and an oxidation flow reactor (OFR). Our results showed that the OA enhancement from OH aging peaked at ∼3.9 equivalent days with an average enhancement of 0.9 (±0.3) µg m-3. Positive matrix factorization analysis of AMS-OFR data identified three primary OA (POA) and two SOA factors. While the concentrations of POA factors decreased as a function of photochemical age, the two SOA factors showed clear enhancements by 2.5 and 4.3 µg m-3 at ∼3.9 and ∼2.6 days of equivalent photochemical age, respectively. The average contribution of SOA to the total OA was 47% in ambient air and 87% in OFR-oxidized ambient air. The elevated oxygen-to-carbon (O/C) ratio from 0.49 to 0.77-0.82 and the decreased hydrogen-to-carbon (H/C) from 1.37 to ∼1.1 highlighted the formation of more oxidized SOA during photochemical aging in winter in Beijing. The ubiquitous SOA enhancement as a function of OA levels indicated the significant formation potential of SOA in winter, and it varied differently among different episodes. In particular, we observed a maximum SOA enhancement of 38.6 µg m-3 during a biomass burning event. This result demonstrates that photochemical oxidation of ubiquitous biomass burning emissions can be a large source of SOA in winter in North China Plain.

Approach to reduce light field sampling redundancy for flame temperature reconstruction.

Flame temperature measurement through a light field camera shows an attractive research interest due to its capabilities of obtaining spatial and angular rays' information by a single exposure. However, the sampling information collected by the light field camera is vast and most of them are redundant. The reconstruction process occupies a larger computing memory and time-consuming. We propose a novel approach i.e., feature rays under-sampling (FRUS) to reduce the light field sampling redundancy and thus improve the reconstruction efficiency. The proposed approach is evaluated through numerical and experimental studies. Effects of under-sampling methods, flame dividing voxels, noise levels and light field camera parameters are investigated. It has been observed that the proposed approach provides better anti-noise ability and reconstruction efficiency. It can be valuable not only for the flame temperature reconstruction but also for other applications such as particle image velocimetry and light field microscope.

Simultaneous Determination of Aerosol Inorganic and Organic Nitrogen by Thermal Evolution and Chemiluminescence Detection.

Inorganic nitrogen (IN) and organic nitrogen (ON) molecules constitute a significant part of atmospheric aerosol. Unlike IN, the total ON quantity remains largely unquantified due to a lack of a simple and direct measurement method. This analytical deficiency hinders the quantitative assessment of the various environmental and health effect impacts by aerosol ON. In this work, we developed an analyzer system that utilizes programmed thermal evolution of carbonaceous and nitrogenous aerosols and chemiluminescence detection coupled with the multivariate curve resolution data treatment to achieve simultaneous quantification of IN and ON. The system is capable of detecting IN and ON as low as 96 ng N per sample on a small filter aliquot (1 cm2) without any pretreatment. This method breakthrough opens the door to quantifying an important pool of aerosol N that was analytically inaccessible in the past and holds the promise to quantifying IN and ON in other environmental samples. As a demonstration, quantification of aerosol ON at an urban site in Hong Kong, China, in samples spanning over a year reveals ON constituting a significant fraction (9-52%) of the total aerosol nitrogen and having major source origins in both secondary formation and primary emissions.

Artisan versus Artiflex phakic intraocular lens implantation in the treatment of moderate to high myopia: meta-analysis.

BACKGROUND: To compare the postoperative safety, efficacy, predictability, visual quality and biomechanics after implantation of Artisan vs. Artiflex phakic intraocular lenses (PIOLs). METHODS: Pubmed, Embase, Cochrane Library were conducted up from January 2000 to February 2020. Comparative clinical studies reporting in accordance with the eligibility criteria were included in this meta-analysis. The pooled weighted mean differences (WMDs) and odds ratios (ORs) with corresponding 95% confidence intervals were calculated. RESULTS: Comparative trials with myopia patients were selected in this review. The pooled WMD and OR estimates statistical significance in terms of postoperative best corrected visual acuity (BCVA), efficacy, postoperative spherical equivalence (SE), predictability, contrast sensitivity and mean intraocular higher-order aberrations (HOA) (mm) for a 6-mm pupil, manifesting that Artiflex PIOL showed evident beneficial effect for correcting myopia compared to Artisan PIOL. There was no significant difference in the incidence of complications between the two groups. CONCLUSION: Both of two techniques were safe and effective for myopia and compared to Artisan PIOL, Artiflex PIOL had significant improvement in efficacy, predictability, contrast sensitivityand HOA, except safety and complications in the treatment of moderate to high myopia.

Design, Synthesis, and Evaluation of Dihydropyranopyrazole Derivatives as Novel PDE2 Inhibitors for the Treatment of Alzheimer's Disease.

Phosphodiesterase 2 (PDE2) has been regarded as a novel target for the treatment of Alzheimer's disease (AD). In this study, we obtained (R)-LZ77 as a hit compound with moderate PDE2 inhibitory activity (IC50 = 261.3 nM) using a high-throughput virtual screening method based on molecular dynamics. Then, we designed and synthesized 28 dihydropyranopyrazole derivatives as PDE2 inhibitors. Among them, compound (+)-11h was the most potent PDE2 inhibitor, with an IC50 value of 41.5 nM. The molecular docking of PDE2-(+)-11h reveals that the 4-(trifluoromethyl)benzyl)oxyl side chain of the compound enters the H-pocket and forms strong hydrophobic interactions with L770/L809/F862, which improves inhibitory activity. The above results may provide insight for further structural optimization of highly potent PDE2 inhibitors and may lay the foundation for their use in the treatment of AD.

The effect of astaxanthin on inflammation in hyperosmolarity of experimental dry eye model in vitro and in vivo.

Hyperosmolarity is pro-inflammatory stress to the ocular surface epithelium associated with dry eye disease (DED). Astaxanthin (AST) is a kind of carotene, which exists in seafood and plays important roles in the amelioration of inflammatory diseases like arteriosclerosis, inflammatory bowel disease, sepsis, rheumatoid arthritis, gastric inflammation, brain inflammatory diseases. The aim of this study was to characterize the protective effect and potential mechanism of AST on DED in vitro and in vivo. Mouse models and human corneal epithelial cell (HCEC) cultures were exposed to hyperosmotic saline solution (HOSS) in in vitro and in vivo experiments, respectively. Experimental subjects were first pretreated with AST, and then the effect of the compound was assessed with clinical evaluation, real-time PCR (RT-PCR), western blot and immunofluorescent staining. We further investigated the possible mechanism of AST in DED by pre-treating with phosphoinositide 3-kinase inhibitor (LY294002). The addition of AST significantly reduced the expression of High-mobility group box 1 (HMGB1), as well as significantly inhibited the increases of TNF-α, IL-1ß in a dose-dependent manner, but promoted the expression of phospho-Akt (p-Akt). BALB/c mice in DE group pretreated with AST showed significantly decreased corneal fluorescein staining scores. Moreover, pretreatment with LY294002 could eliminate the effects of AST preconditioning on the decrease of HMGB1. Our study provides evidence that AST could ameliorate DED which may be related to the inhibition of HMGB1, TNF-α, IL-1ß, while PI3K/Akt signaling pathway may be involved in the expression of HMGB1 and the protective effect of AST preconditioning.

Passively Q-switched Nd-doped fiber laser based on PbS/CdS core/shell quantum dots as a saturable absorber.

In our work, PbS/CdS core/shell quantum dots with an absorption peak of 1043 nm were successfully employed as a modulator for achieving a passively Q-switched Nd-doped fiber laser. The saturation intensity and modulation depth of the film-type modulator were 7.6 MW/cm2 and 4.1%, respectively. Due to the protection of the CdS shell, the PbS core exhibited good photo-chemical stability, which led to the generation of stable passively Q-switched operation. The maximum average output power was 7.88 mW with a minimum pulse width of 235.7 ns. To our knowledge, this was the first demonstration focusing on the combination of quantum dot and Nd-doped fiber laser; in addition, 235.7 ns was the narrowest pulse width of a passively Q-switched Nd-doped fiber laser. Our results highlighted the excellent nonlinear absorption properties of PbS/CdS core/shell quantum dots and give significant guidance for future optical applications of quantum dots and demonstrations of pulsed Nd-doped fiber lasers.

Redistributing the energy flow of a tightly focused radially polarized optical field by designing phase masks.

Redistributing the transverse energy flow in the focal plane of a tightly focused radially polarized optical field is described. We develop from theory a generalized analytical model for calculating the distributions of the electromagnetic field and the Poynting vector for a tightly focused radially polarized laser beam superposed with an optical vortex. We further explore the redistribution of the energy flow by designing phase masks, including traditional and annular vortex phase masks. Flexible control of the transverse energy flow rings is obtained with these phase masks. They provide a simple solution to transport absorptive particles along certain paths and therefore might be help in optical tweezer manipulations.

Focusing properties of arbitrary optical fields combining spiral phase and cylindrically symmetric state of polarization.

The tight focusing properties of optical fields combining a spiral phase and cylindrically symmetric state of polarization are presented. First, we theoretically analyze the mathematical characterization, Stokes parameters, and Poincaré sphere representations of arbitrary cylindrical vector (CV) vortex beams. Then, based on the vector diffraction theory, we derive and build an integrated analytical model to calculate the electromagnetic field and Poynting vector distributions of the input CV vortex beams. The calculations reveal that a generalized CV vortex beam can generate a sharper focal spot than that of a radially polarized (RP) plane beam in the focal plane. Besides, the focal size decrease accompanies its elongation along the optical axis. Hence, it seems that there is a trade-off between the transverse and axial resolutions. In addition, under the precondition that the absolute values between polarization order and topological charge are equal, a higher-order CV vortex can also achieve a smaller focal size than an RP plane beam. Further, the intensity for the sidelobe admits a significant suppression. To give a deep understanding of the peculiar focusing properties, the magnetic field and Poynting vector distributions are also demonstrated in detail. These properties may be helpful in applications such as optical trapping and manipulation of particles and superresolution microscopy imaging.

Focus shaping by tailoring arbitrary hybrid polarization states that have a combination of orthogonal linear polarization bases.

We report a focus shaping method by tailoring hybrid states of polarization of arbitrary polarized beams that have a combination of orthogonal linear polarization bases. Such hybridly polarized beams comprising linear, elliptical, and circular polarizations in the beam cross section, have completely different optical properties compared to the scalar and locally linear-polarized vector beams. We demonstrate that, apart from the orientation of the local polarization state, another two degrees of freedom including the local ellipticity and the handedness in the beam cross section can be used in focus shaping. Square-shaped patterns, multiple foci, three-dimensional optical cages, optical needles, and channels can be obtained due to the increased control without any additional phase or amplitude modulations.

Optical cage generated by azimuthal- and radial-variant vector beams.

We propose a method to generate an optical cage using azimuthal- and radial-variant vector beams in a high numerical aperture optical system. A new kind of vector beam that has azimuthal- and radial-variant polarization states is proposed and demonstrated theoretically. Then, an integrated analytical model to calculate the electromagnetic field and Poynting vector distributions of the input azimuthal- and radial-variant vector beams is derived and built based on the vector diffraction theory of Richards and Wolf. From calculations, a full polarization-controlled optical cage is obtained by simply tailoring the radial index of the polarization, the uniformity U of which is up to 0.7748, and the cleanness C is zero. Additionally, a perfect optical cage can be achieved with U=1, and C=0 by introducing an amplitude modulation; its magnetic field and energy flow are also demonstrated in detail. Such optical cages may be helpful in applications such as optical trapping and high-resolution imaging.

A Cooperative Traffic Control of Vehicle-Intersection (CTCVI) for the Reduction of Traffic Delays and Fuel Consumption.

The problem of reducing traffic delays and decreasing fuel consumption simultaneously in a network of intersections without traffic lights is solved by a cooperative traffic control algorithm, where the cooperation is executed based on the connection of Vehicle-to-Infrastructure (V2I). This resolution of the problem contains two main steps. The first step concerns the itinerary of which intersections are chosen by vehicles to arrive at their destination from their starting point. Based on the principle of minimal travel distance, each vehicle chooses its itinerary dynamically based on the traffic loads in the adjacent intersections. The second step is related to the following proposed cooperative procedures to allow vehicles to pass through each intersection rapidly and economically: on one hand, according to the real-time information sent by vehicles via V2I in the edge of the communication zone, each intersection applies Dynamic Programming (DP) to cooperatively optimize the vehicle passing sequence with minimal traffic delays so that the vehicles may rapidly pass the intersection under the relevant safety constraints; on the other hand, after receiving this sequence, each vehicle finds the optimal speed profiles with the minimal fuel consumption by an exhaustive search. The simulation results reveal that the proposed algorithm can significantly reduce both travel delays and fuel consumption compared with other papers under different traffic volumes.

Causal relationship between lactate dehydrogenase and risk of developing ischemic stroke: A Mendelian randomized study.

BACKGROUND AND OBJECTIVE: Ischemic stroke (IS) is one of the major global health problems. It is not clear whether there is a causal relationship between lactate dehydrogenase (LDH) and the risk of IS attacks. The purpose of this study was to investigate whether LDH has a causal relationship with the development of IS. METHODS: The genome-wide association data of LDH and IS were obtained through a Mendelian randomization-based platform. Single nucleotide polymorphisms (SNP) that were significantly associated with LDH were identified and used as instrumental variables, and a two-sample Mendelian randomization study was used to examine the causal relationship between LDH and IS. The statistical methods included Inverse-variance weighted approach, MR-Egger regression, and weighted median estimator. RESULTS: We selected 15 SNPs of genome-wide significance from Genome-wide association study database with LDH as instrumental variables. A consistent causal association between LDH and IS was observed by different assessment methods. The results of the inverse-variance weighted method suggested an inverse association between LDH and higher genetic predictability of IS risk (OR, 0.997; 95%CI 0.995-0.999). The weighted median estimate showed consistent results with the MR-Egger method (weighted median estimate: OR, 0.995; 95%CI 0.992-0.999; MR-Egger method: OR, 0.996; 95%CI 0.992-0.999). The inverse-variance weighted method indicates a causal association between LDH and IS (ß = -0.002563, SE = 0.00128, p = .0453). MR-Egger analysis (ß = -0.004498, SE = 0.001877, p = .03) and the weighted median method suggested that LDH and IS also existed causal relationship (ß = -0.004861, SE = 0.001801, p = .00695). CONCLUSIONS: Our Mendelian randomization results suggest that LDH is inversely associated with the risk of developing IS, and are contrary to the results of previous observational studies.