Function and regulation of miR-186-5p, miR-125b-5p and miR-1260a in chordoma.

BACKGROUND: The function and regulation of miRNAs in progression of chordoma were unclear. METHODS: Five miRNAs were identified by the machine learning method from the miRNA expression array. CCk-8 assay, EDU assay, wound healing migration assay, and trans-well assay were used to reveal the effect of the miRNAs in chordoma cell lines. Moreover, bioinformation analysis and the mRNA expression array between the primary chordomas and recurrent chordomas were used to find the target protein genes of miRNAs. Furthermore, qRT-PCR and luciferase reporter assay were used to verify the result. RESULTS: miR-186-5p, miR-30c-5p, miR-151b, and miR-125b-5p could inhibit proliferation, migration, and invasion of chordoma while miR-1260a enhances proliferation, migration, and invasion of chordoma. Recurrent chordoma has a worse disease-free outcome than the primary chordoma patients. AMOT, NPTX1, RYR3, and P2RX5 were the target protein mRNAs of miR-186-5p; NPTX1 was the target protein mRNAs of miR-125b-5p; and AMOT and TNFSF14 were the target protein mRNAs of miR-1260a. CONCLUSIONS: miR-186-5p, miR-125b-5p, miR-1260a, and their target protein mRNAs including AMOT, NPTX1, RYR3, P2RX5, TNFSF14 may be the basement of chordoma research.

An ab initio study on the electronic excited states and photodissociation mechanism of bromocarbene molecule.

We used the internally contracted explicitly correlated multireference configuration interaction (icMRCI-F12) method combined with Davidson correction to conduct a high-precision ab initio study of CHBr. The spin-orbit coupling (SOC) is incorporated into the calculation. The 21 spin-free states split into 53 spin-coupled states of CHBr. The vertical transition energies and oscillator strengths are obtained of these states. The SOC effect on the equilibrium structures and the harmonic vibrational frequencies of the ground state X1A', the lowest triplet state a3A'' and the first excited singlet state A1A'' is investigated. The results reveal a significant effect of the SOC on the bond angle and the frequency of the bending mode of a3A''. The potential energy curves of electronic states of CHBr as functions of the H-C-Br bond angle, C-H bond length, and C-Br bond length, respectively, are also investigated. Based on the calculated results, the interactions between electronic states and photodissociation mechanism involved in CHBr in the ultraviolet region are explored. Our theoretical studies will shed light on the complicated interactions and dynamics of the electronic states of bromocarbenes.

Microglial activation and pyroptosis induced by nano-TiO2 in marine medaka brain.

Recently, nano-titanium dioxide (nano-TiO2) has been widely distributed over surface water. However, there are few reports on its effects on the central nervous system of fish. In this study, we investigated whether nano-TiO2 enters the medaka brain after exposure and its effect on the brain. Marine medaka brains were examined after exposure to 0.01 g/L nano-TiO2 for 3, 10, and 20 d. Nano-TiO2-like particles were found in the telencephalon of treated fish. There was no obvious brain histopathological injury. The number of irregular mitochondria with absent cristae increased. Gene expression of the apoptosis-related genes, casp8, bcl2b, and bax, decreased significantly in the nano-TiO2 group at 3 d. In contrast, the pyroptosis-related genes, gsdmeb and casp1, and inflammation-related factor, il18, increased significantly. As an activated microglia marker, mRNA expression of cd68 increased significantly in the nano-TiO2 treated group. Moreover, CD68 protein expression also increased significantly at 10 d. Altogether, we show that nano-TiO2 can alter mitochondrial morphology in the telencephalon of medaka, leading to microglial activation and pyroptosis.

Magnetization reversal of perpendicular magnetic anisotropy regulated by ferroelectric polarization in CoFe3N/BaTiO3 heterostructures: first-principles calculations.

Exploring the electric-field switching of perpendicular magnetic anisotropy (PMA) in multiferroic heterostructures has important physical significance, which attracts great interest due to its promising application for energy-efficient information storage. Herewith, we investigate the effect of ferroelectric polarization on magnetic anisotropy in CoFe3N/BaTiO3 heterostructures using first-principles calculations. The calculations reveal that the magnetic anisotropy of CoFe3N can be regulated by ferroelectric polarization of BaTiO3. When the ferroelectric polarization reverses, the PMA of FeCo-TiO2 and FeN-BaO configurations remains, but in the FeN-TiO2 and FeCo-BaO cases, magnetic anisotropy inverses between out-of-plane and in-plane direction. Further orbital-resolved analysis indicates that the transition of magnetic anisotropy is mainly attributed to the orbital hybridization of interfacial Fe/Co atoms with O atoms induced by the magnetoelectric effect. This study may open an effective approach toward modulating PMA and lays a foundation to the development of low energy consumption memory devices.

Flexible and Giant Terahertz Modulation Based on Ultra-Strain-Sensitive Conductive Polymer Composites.

Dynamic tuning of terahertz (THz) wave has a great potential application as smart THz devices, such as switches, modulators, sensors, and so on. However, the realization of flexible THz modulation with high efficiency is rarely observed, which is nearly absent from the booming development and demands on flexible electronics. Here, we report a flexible THz modulation based on conductive polymer composites composed of thermoplastic polyurethane (TPU) and conductive particles (Ni). By designing the additive content of Ni particles, such a flexible layer exhibits resistivity change of 6-7 orders under tensile strain due to the formation of an electron-transport channel provided by the in situ evolution of the Ni network. It could be used to dynamically control the THz transmission with a giant modulation depth of around 96%, at a high strain operation (up to around 58.5%). Moreover, these characteristics are demonstrated to be available for highly tension sensitive THz spectroscopy and imaging. This work opens up a connection between flexible polymer-based composites and THz dynamic devices. It proposes an unprecedented flexible THz modulation with giant tuning efficiency and provides a scheme for contactless and passive tension sensors.

Orbital Redistribution Enhanced Perpendicular Magnetic Anisotropy of CoFe3N Nitrides by Adsorbing Organic Molecules.

Organic/ferromagnetic spinterface plays a significant role in organic spintronics and the manipulation of spinterface will help to optimize the performance of molecular devices. Here, we systematically investigate how the magnetic anisotropy can be tailed by adsorbing different organic molecules on CoFe3N surface. It is found that the adsorption of C6H6, C6F6, and SC4H4 molecules on the FeCo-hollow site enhances the perpendicular magnetic anisotropy (PMA) of CoFe3N. The redistribution of Fe/Co d-orbitals near the Fermi level has an important effect on the modulation of PMA. Asymmetric SC4H4 adsorbed system has a larger PMA than symmetric C6H6 and its halide due to the hybridization between S p z and Fe d z2 orbitals instead of C atom. Our results indicate that appropriate organic molecule adsorption can improve the magnetic properties of ferromagnets, which benefits organic spintronic devices.

Interfacial exchange coupling induced anomalous anisotropic magnetoresistance in epitaxial γ'-Fe4N/CoN bilayers.

Anisotropic magnetoresistance (AMR) of the facing-target reactively sputtered epitaxial γ'-Fe4N/CoN bilayers is investigated. The phase shift and rectangular-like AMR appears at low temperatures, which can be ascribed to the interfacial exchange coupling. The phase shift comes from the exchange bias (EB) that makes the magnetization lag behind a small field. When the γ'-Fe4N thickness increases, the rectangular-like AMR appears. The rectangular-like AMR should be from the combined contributions including the EB-induced unidirectional anisotropy, intrinsic AMR of γ'-Fe4N layer and interfacial spin scattering.