[Study on the expression of cytokeratin 19 mRNA in oral squamous cell carcinoma].

OBJECTIVE: To elucidate the possible mechanism of oral carcinogenesis and to explore the value of clinical application of the detection of cytokeratin (CK) 19 for oral squamous cell carcinoma (OSCC) patients. METHODS: The cancerous tissues, para-cancerous tissues and excised lymph nodes were collected from 20 operated patients with OSCC. The patients didn't receive radiotherapy and chemotherapy before hospitalization. The relative expression of CK19 mRNA in those tissues was detected by fluorescent quantitative polymerase chain reaction (FQ-PCR). RESULTS: The expression of CK19 mRNA in the cancerous tissues was 1.85 and 1.66 times higher than that in normal oral mucosa and in para-cancerous tissues, respectively. The expression of CK19 mRNA in lymph nodes from 9 patients with OSCC was positive and the positive rate was 45% (9/20). The positive rate of CK19 mRNA in all lymph nodes from 9 patients with OSCC was 81.8% (18/22), and the positive rate of CK19 mRNA in all lymph nodes from 20 patients with OSCC was 41.9%(18/43). CK19 mRNA level in the cancerous tissues relative to para-cancerous tissues and normal oral mucosa of the patients whose CK19 mRNA expression was positive was lower than that of the patients whose CK19 mRNA expression was negative in lymph nodes, respectively. CONCLUSION: The possible reason that the expression of CK19 mRNA in the cancerous tissues was higher than that in para-cancerous tissues and normal oral mucosa was that the CK19 synthesis in cancerous tissues increased obviously. The detection of CK19 mRNA in lymph nodes was regarded probably as one of the markers for detecting OSCC micrometastasis in lymph nodes. The detection of CK19 mRNA in lymph nodes by FQ-PCR was more sensitive than hematoxylin-eosin staining in diagnosing OSCC micrometastasis.

[Association of muscle segment homeobox gene 1 polymorphisms with nonsyndromic cleft lip with or without cleft palate].

OBJECTIVE: Muscle segment homeobox gene (MSX)1 has been proposed as a gene in which mutations may contribute to nonsyndromic cleft lip with or without cleft palate (NSCL/P). To study MSX1 polymorphisms in NSCL/ P by means of polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP), and investigate the association of MSX1 exons 1 polymorphisms with NSCL/P. METHODS: DNA were extracted from blood samples from NSCL/P and unrelated normal subjects. Genome DNA from peripheral leukocyte with these blood samples were extracted, which was used as template to amplify desired gene fragment of MSX1 exons 1 by means of polymerase chain reaction (PCR). The PCR products were examined by single-strand conformation polymorphism (SSCP). The MSX1 exons 1 polymorphisms were examined by sequencing if mutations were found. RESULTS: MSX1 genes of exon 1 mutation was not been found in the NSCL/P and unrelated normal subjects by SSCP. CONCLUSION: No correlation between MSX1 exon 1 and NSCL/P was found. MSX1 exon 1 may not be a key gene (susceptibility gene) in NSCL/P.

[The expression of Maspin protein in oral squamous cell carcinoma and its significance].

OBJECTIVE: To investigate the role of Maspin protein in the development of oral squamous cell carcinoma (OSCC), with the effort to realize practical diagnosis use of them. METHODS: The expression status of Maspin protein in 45 cases of OSCC, 33 cases of paratumor tissue and 15 cases of normal tissue was examined by immunohistochemical staining. RESULTS: The positive rates of Maspin protein were 86.67% (13/15), 72.73% (24/33) and 37.78% (17/45) in normal tissue, paratumor tissue and OSCC. The expression of Maspin protein increased respectively in OSCC, paratumor tissue and normal tissue. The expression were different between OSCC and normal tissue, OSCC and paratumor tissue, paratumor tissue and normal tissue (P<0.05). The expression of Maspin protein was correlated with lymph node metastasis, histological grade (P<0.05), but not with TNM staging (P>0.05). CONCLUSION: Maspin protein may play an important role in the development of OSCC. The expression level of Maspin protein detected in early time might be a useful prognostic marker for the diagnosis of lymph node metastasis.

[A case report of infantile myofibromatosis of left mandibular angle].

The clinical data of one case of infantile myofibromatosis of left mandibular angle were analyzed, and the clinicopathological characteristics, imaging diagnosis, treatment and prognosis of infantile myofibromatosis were discussed.