RESUMEN
Enamel is a unique tissue in vertebrates, acellular, formed on a labile scaffolding matrix and hypermineralized. The ameloblasts are epithelial cells in charge of amelogenesis. They secrete a number of matrix proteins degraded by enzymes during enamel mineralization. This ordered cellular and extracellular events imply that any genetic or environmental perturbation will produce indelible and recognizable defects. The specificity of defects will indicate the affected cellular process. Thus, depending on the specificity of alterations, the teratogenic event can be retrospectively established. Advances in the field allow to use enamel defects as diagnostic tools for molecular disorders. The multifunctionality of enamel peptides is presently identified from their chemical roles in mineralization to cell signaling, constituting a source of concrete innovations in regenerative medicine.
Asunto(s)
Esmalte Dental/fisiología , Ameloblastos/citología , Ameloblastos/metabolismo , Amelogénesis/fisiología , Animales , Esmalte Dental/química , Esmalte Dental/efectos de los fármacos , Esmalte Dental/ultraestructura , Hipoplasia del Esmalte Dental/genética , Hipoplasia del Esmalte Dental/fisiopatología , Proteínas del Esmalte Dental/fisiología , Durapatita/química , Órgano del Esmalte/fisiología , Fluorosis Dental/etiología , Humanos , Técnicas de Diagnóstico Molecular , Nanosferas , Péptido Hidrolasas/fisiología , Teratógenos/farmacología , Calcificación de Dientes/fisiologíaAsunto(s)
Amelogénesis Imperfecta , Calcificación Fisiológica/genética , Proteínas del Esmalte Dental , Pulpa Dental , Mutación , Nefrocalcinosis , Adolescente , Adulto , Amelogénesis Imperfecta/genética , Amelogénesis Imperfecta/metabolismo , Proteínas del Esmalte Dental/genética , Proteínas del Esmalte Dental/metabolismo , Pulpa Dental/química , Pulpa Dental/metabolismo , Femenino , Humanos , Masculino , Nefrocalcinosis/genética , Nefrocalcinosis/metabolismoRESUMEN
Background and objective:FAM20A gene mutations result in enamel renal syndrome (ERS) associated with amelogenesis imperfecta (AI), nephrocalcinosis, gingival fibromatosis, and impaired tooth eruption. FAM20A would control the phosphorylation of enamel peptides and thus enamel mineralization. Here, we characterized the structure and chemical composition of unerupted tooth enamel from ERS patients and healthy subjects. Methods: Tooth sections were analyzed by Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), X-Ray Diffraction (XRD), and X-Ray Fluorescence (XRF). Results: SEM revealed that prisms were restricted to the inner-most enamel zones. The bulk of the mineralized matter covering the crown was formed by layers with varying electron-densities organized into lamellae and micronodules. Tissue porosity progressively increased at the periphery, ending with loose and unfused nanonodules also observed in the adjoining soft tissues. Thus, the enamel layer covering the dentin in all ERS patients (except a limited layer of enamel at the dentino-enamel junction) displayed an ultrastructural globular pattern similar to one observed in ectopic mineralization of soft tissue, notably in the gingiva of Fam20a knockout mice. XRD analysis confirmed the existence of alterations in crystallinity and composition (vs. sound enamel). XRF identified lower levels of calcium and phosphorus in ERS enamel. Finally, EDS confirmed the reduced amount of calcium in ERS enamel, which appeared similar to dentin. Conclusion: This study suggests that, after an initial normal start to amelogenesis, the bulk of the tissue covering coronal dentin would be formed by different mechanisms based on nano- to micro-nodule aggregation. This evocated ectopic mineralization process is known to intervene in several soft tissues in FAM20A gene mutant.