RESUMEN
The genus Orbivirus of the family Reoviridae comprises 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analysis by electron microscopy and gel electrophoresis, respectively, revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analysis support previous serological findings, which indicate that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, six out of 13 analysed CGLV serotypes showed reassortment of their genome segments.
Asunto(s)
Genoma Viral , Orbivirus/genética , Orbivirus/fisiología , ARN Viral/genética , Análisis de Secuencia de ADN , Animales , Brasil , Análisis por Conglomerados , Electroforesis , Orden Génico , Humanos , Insectos , Microscopía Electrónica , Datos de Secuencia Molecular , Orbivirus/química , Orbivirus/ultraestructura , Filogenia , Proteínas Estructurales Virales/análisis , Virión/ultraestructuraRESUMEN
Globally, yellow fever virus infects nearly 200,000 people, leading to 30,000 deaths annually. Although the virus is endemic to Latin America, only a single genome from this region has been sequenced. Here, we report 12 Brazilian yellow fever virus complete genomes, their genetic traits, phylogenetic characterization, and phylogeographic dynamics. Variable 3' noncoding region (3'NCR) patterns and specific mutations throughout the open reading frame altered predicted secondary structures. Our findings suggest that whereas the introduction of yellow fever virus in Brazil led to genotype I-predominant dispersal throughout South and Central Americas, genotype II remained confined to Bolivia, Peru, and the western Brazilian Amazon.
Asunto(s)
Genoma Viral , Filogenia , Virus de la Fiebre Amarilla/genética , Secuencia de Bases , Brasil , Cartilla de ADN , Glicosilación , Reacción en Cadena de la Polimerasa , Virus de la Fiebre Amarilla/clasificaciónRESUMEN
This is the first announcement of two nearly complete viral genome sequences belonging to the Guama serogroup (genus Orthobunyavirus, family Bunyaviridae) isolated in the Brazilian Amazon region: Mirim virus (MIRV; BEAN7722) and Ananindeua virus (ANUV; BEAN109303).
RESUMEN
We report here nearly complete genome sequence of Inhangapi virus (INHV) strain BEAR177325, which was isolated from a pool of sandflies (Lutzomyia flaviscutellata) in the Utinga neighborhood, Belém (01º28´S 48°27'W), State of Pará, Brazil, in 1969. The genome of this virus showed similarity with members belonging to the family Rhabdoviridae.
RESUMEN
Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi), as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas.
Asunto(s)
Técnicas de Amplificación de Ácido Nucleico/métodos , Proteínas no Estructurales Virales/genética , Virus de la Fiebre Amarilla/aislamiento & purificación , África , Secuencia de Bases , Región del Caribe , América Central , Cartilla de ADN , Humanos , Datos de Secuencia Molecular , Vigilancia de la Población/métodos , América del Sur , Fiebre Amarilla/virologíaAsunto(s)
Farmacorresistencia Viral/genética , Infecciones por VIH/epidemiología , Integrasa de VIH/genética , VIH-1/efectos de los fármacos , VIH-1/enzimología , Adulto , Fármacos Anti-VIH , Brasil/epidemiología , Femenino , Infecciones por VIH/tratamiento farmacológico , Humanos , Masculino , Mutación , Prevalencia , Adulto JovenRESUMEN
We describe here the nearly complete open reading frame (ORF) of five Gamboa virus strains isolated in Panama and Argentina. The viruses with complete ORF showed the regular genome organization observed in other orthobunyaviruses with exception to the presence of NSs protein. All predicted proteins showed homology with viruses belonging to members of the family Bunyaviridae.