Klebsiella aerogenes exacerbates colon tumorigenesis in the AOM/DSS-induced C57BL/6J mouse.

Klebsiella aerogenes (K. aerogenes, KA) is a gram-negative opportunistic pathogen from the Klebsiella species and the Enterobacteriaceae family. However, the impact of K. aerogenes on colorectal cancer (CRC) remains uncertain. A colitis-associated tumorigenesis animal model was established by administering azoxymethane (AOM) and dextran sulfate sodium (DSS) to C57BL/6J mice. The concentration of K. aerogenes gavage in mice was 109 cfu. The study measured the following parameters: tumor formation (number and size), intestinal permeability (MUC2, ZO-1, and Occludin), colonic inflammation (TNF-α, IL-1ß, IL-6, and IL-10), proliferation and the fluctuation of the intestinal flora. Under the AOM/DSS-treated setting, K. aerogenes colonization worsened colitis by exacerbating intestinal inflammatory reaction and destroying the mucosal barrier. The intervention markedly augmented the quantity and dimensions of neoplasm in the AOM/DSS mice, stimulated cellular growth, and impeded cellular programmed cell death. In addition, K. aerogenes exacerbated the imbalance of the intestinal microbiota by elevating the abundance of Pseudomonas, Erysipelatoclostridium, Turicibacter, Rikenella, and Muribaculum and leading to a reduction in the abundance of Odoribacter, Alloprevotella, Roseburia, and Lachnospiraceae_NK4A136_group. The presence of K. aerogenes in AOM/DSS-treated mice promoted tumorigenesis, worsened intestinal inflammation, disrupted the intestinal barrier, and caused disturbance to the gut microbiota.

Wastewater treatment: A universal, scalable and recyclable catalyst with adjustable activity for diverse dyes degradation.

The growing concern over water shortage and pollution is propelling and accelerating the development of sewage treatment technologies. Among them, the catalytic hydrogenation method is highly recommended from a sustainable perspective, because it can turn toxic pollutants into valuable raw materials. The catalyst with excellent activity and stability plays a critical role in this "trash to treasure" approach. Herein, we proposed a novel economical, scalable and recyclable candidate catalyst, i.e., the copper nanoparticles supported on zinc oxide nanowire array (Cu-ZnO NWA), for realizing efficient and stable dye wastewater treatment. The salix argyracea-shaped Cu-ZnO NWA displays very outstanding universality and controllability towards the catalytic hydrogenation reactions of diverse dyes, owing to the fact that ZnO nanowire array not only offers a platform to realize stable and homogeneous dispersion of Cu nanoparticles, but also provides a large quantity of catalytically active sites. More attractively, its synthetic method can be facilely extended to various conductive substrates through combined electrodeposition and hydrothermal technique, showing its general applicability for the surface assembly of sewage treatment facilities. Benefiting from above advantages, this proposal offers an attractive approach for large-scale and continuous decolorization of dye wastewater, and presents a broad application prospect in the textile printing industry.

Review of the emGekko/em (emJaponigekko/em) emsubpalmatus/em complex (Squamata, Sauria, Gekkonidae), with description of a new species from China.

The gecko species Gekko (Japonigekko) subpalmatus was previously recorded with a relatively wide distribution from eastern, southern, and southeastern China. However, the populations in southern China are currently recognized as another valid species G. (J.) melli. In this study, we conduct a detailed morphological examination and phylogenetic analysis of the populations currently treated as G. (J.) subpalmatus or G. (J.) melli, which are collectively designated as the G. (J.) subpalmatus complex. Our results reveal that the G. (J.) subpalmatus complex comprises three evolutionarily independent taxa. The populations from Zhejiang, eastern China are G. (J.) subpalmatus, those from southern China are G. (J.) melli, while those from the Sichuan Basin, southwestern China represent a cryptic species, Gekko (Japonigekko) cib sp. nov.. Gekko (Japonigekko) cib sp. nov. can be distinguished from all congeners, by its divergence from other complex members in the CYTB and 16S genes, and a combination of morphological characteristics, especially in hemipenial morphology. Historic records of G. (J.) subpalmatus complex are also reviewed.

Re-examination of the Chinese record of Opisthotropis maculosa (Squamata, Natricidae), resulting in the first national record of O. haihaensis and description of a new species.

The taxonomic status of the previous record of Opisthotropis maculosa Stuart & Chuaynkern, 2007 from Guangdong and Guangxi, southern China, is revised based on the comparison of morphological and molecular data collected from the Chinese specimens and the holotype of O. maculosa from Thailand and O. haihaensis Ziegler, Pham, Nguyen, Nguyen, Wang, Wang, Stuart & Le, 2019 from Vietnam. Results reveal that the population from Shiwandashan Nature Reserve in southern Guangxi, China belongs to O. haihaensis, and represents the first national record for China; the populations from western Guangdong and southeastern Guangxi are described as a new species, Opisthotropis hungtai sp. nov. We suggest that O. maculosa should be removed from the Chinese herpetofauna checklist. The new national record of O. haihaensis and the description of the new species bring the total number of Opisthotropis to 13 in China.

A new species of the genus Achalinus from southwestern Guangdong Province, China (Squamata: Xenodermatidae).

A new species of xenodermatid snake, Achalinus yunkaiensis J. Wang, Y. Li Y.Y. Wang, sp. nov. was described based on a series of specimens collected from Dawuling Forestry Station located in the Yunkai Mountains of western Guangdong Province. It can be distinguished from known congeners by a significant genetic divergence at the mitochondrial CO1 gene fragment examined (p-distance ≥ 12.0%) and the following combination of characters: (1) dorsal scales strongly keeled, in 23 rows throughout the body, the most outer row on each side smooth and significantly enlarged; (2) tail relatively shorter, TaL/TL ratio 18.5-20.0%; (3) maxillary teeth 20-22; (4) length of suture between internasals subequal to that between the prefrontals; (5) nasal divided into two sections by nasal cleft, posterior one half as long as anterior; (6) loreal elongated, nearly twice as wide as high; (7) supralabials six; (8) infralabials six; (9) temporals 2+2+3 (rarely 2+2+4), the two anterior temporals in contact with eye; (10) ventrals 151-162, subcaudals 49-56 arranged in single row, not paired; (11) cloacal entire; (12) uniform brown (in adults) or black (in juveniles) above, tinged weakly iridescent, with a longitudinal dark-colored vertebral line; (13) light brown (in adults) or greyish white (in juveniles) beneath; and (14) dorsum with a longitudinal dark brown vertebral stripe from posterior margin of parietals to tail tip. Currently, 13 species are known in the genus Achalinus, with seven from mainland China.

Outcome of hypospadias reoperation based on preoperative antimicrobial prophylaxis.

From August 1981 to June 2004, we retrospectively reviewed 66 hypospadias, treated with 123 operations, to analyze the outcome of reoperation based on preoperative antimicrobial prophylaxis. All patients received primary treatment in our hospital, and all had postoperative antimicrobial prophylaxis in all surgical procedures. We define a hypopadias repair as "corrected without fistula" if no urethrocutaneous fistula formation, or complete wound disruption is found within at least a 2-week follow-up period. Of the 123 operations, there were 66 primary repairs and 57 reoperations. The mean age at primary repair was 4.33 years. The overall rate of correction without fistula was 53.0% (35/66), with a mean of 14 months, follow-up. A total of 88 failure repairs resulted from 67 (76.0%) fistulas and 21 (24.0%) disruptions. A higher rate of correction without fistula (81.0%) was related to subcoronal hypospadias (p = 0.020) in three types. The rate of correction without fistula of two-staged repair was lowest in primary operations (12.5%), but these cases had a significantly higher rate of correction without fistula in following reoperations (66.7%, p = 0.043). Of the 123 repairs, only 46 (37%) had preoperative antibiotic prophylaxis, and these had lower early complication rates, both in primary and reoperation groups (42.3% vs 52.5% and 40.0% vs 54.1%, respectively). The differences, however, were not statistically significant (p = 0.231 and p = 0.289, respectively). In terms of rate of correction without fistula, a higher rate of correction without fistula was observed in reoperations with preoperative antibiotic prophylaxis (57.1%), compared to those without prophylaxis (23.1%). This difference was statistically significant (p = 0.031). The data from this study suggest that a lower early complication rate and a significantly higher rate of correction without fistula are related to the hypospadias reoperations with preoperative antimicrobial prophylaxis. The use of broad-spectrum antibiotics before surgery is recommended for these cases. However, more precise and prospective studies are warranted.

Feminizing adrenocortical adenoma presenting as heterosexual precocious puberty: report of one case.

We report on a case of a 2 2/12-year-old boy with heterosexual precocious puberty secondary to a feminizing adrenocortical adenoma. The boy, with no previous history of disease or treatment, presented with bilateral gynecomastia and pubic hair development (Tanner III breasts and Tanner II pubic hair). Plasma estradiol and testosterone were 410.9 pg/ml and 126.2 ng/dl respectively. Basal plasma LH and FSH levels were within the normal range. Bolus i.v. injection of GnRH showed unresponsiveness of LH and FSH. Abdominal echography and abdominal magnetic resonance imaging revealed a well-defined mass at the left suprarenal region (measuring 4.0 x 2.7 x 3.6 cm in size). After removal of the adrenal tumor, the estradiol and testosterone levels fell to normal in 2 weeks. The gynecomastia and pubic hair regressed with time. The pathology of the tumor showed compact pattern with polygonal cells containing moderate eosinophilic cytoplasm without mitotic figure. These findings were consistent with an adrenocortical adenoma secreting estradiol and testosterone as the cause of the patient's heterosexual precocious puberty.

Impact of the lips for biometrics.

In this paper, the impact of the lips for identity recognition is investigated. In fact, it is a challenging issue for identity recognition solely by the lips. In the first stage of the proposed system, a fast box filtering is proposed to generate a noise-free source with high processing efficiency. Afterward, five various mouth corners are detected through the proposed system, in which it is also able to resist shadow, beard, and rotation problems. For the feature extraction, two geometric ratios and ten parabolic-related parameters are adopted for further recognition through the support vector machine. Experimental results demonstrate that, when the number of subjects is fewer or equal to 29, the correct accept rate (CAR) is greater than 98%, and the false accept rate (FAR) is smaller than 0.066%. (CAR > 95.02%, FAR < 0.095% # Subjects ≤ 57). Moreover, the processing speed of the overall system achieves 34.43 frames per second, which meets the real-time requirement. Thus, the proposed system can be an effective candidate for facial biometrics applications when other facial organs are covered or when it is applied for an access control system.

Amphetamine inhibits the N-methyl-D-aspartate receptor-mediated responses by directly interacting with the receptor/channel complex.

Amphetamine (AMPH) induces behavioral sensitization and neurotoxicity primarily by enhancing the dopamine-mediated neurotransmission. However, the involvement of the N-methyl-D-aspartate (NMDA) receptor in AMPH-induced neuropathology is also known. Recent investigation has found that high concentration of dopamine could inhibit NMDA receptor-mediated responses by blocking the NMDA receptor channel. By virtue of the structure similarity between dopamine and AMPH, we determined whether d-AMPH and its analogs, l-AMPH and methamphetamine (MAMH), could affect the NMDA receptor-mediated [3H]N-[1-(2-thienyl)cyclohexyl] piperidine ([3H]TCP) binding in rat cortical membrane preparations and intracellular 45Ca2+ accumulation and cell death in the rat primary cortical cell cultures. AMPH concentration-dependently inhibited NMDA- and glycine-stimulated [3H]TCP binding and intracellular 45Ca2+ accumulation with two distinct potencies; a minor inhibition with high potency and a major inhibition with low potency. [3H]TCP binding suggested that the high-potency inhibition was produced by decreasing agonist-induced activation of the NMDA receptor channel. On the other hand, the low-potency inhibition was produced by competing with [3H]TCP binding in the NMDA receptor channel, like the action of noncompetitive antagonist of the NMDA receptor. However, AMPH analogs were less potent in inhibiting NMDA- and glycine-induced cultured cell death. Thus, this result indicates that AMPH could antagonize the NMDA receptor-mediated responses in vitro by two different mechanisms, probably, through directly interacting with two distinct sites on this receptor/channel complex.