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Adipic acid (AA) is an important feedstock for nylon polymers and is industrially produced from fossil-derived aromatics via thermocatalysis. However, this process consumes explosive H2 and corrosive HNO3 as reductants and oxidants, respectively. Here, we report the direct synthesis of AA from lignin-derived phenolic compounds via paired electrolysis using bimetallic cooperative catalysts. At the cathode, phenol is hydrogenated on PtAu catalysts to form ketone-alcohol (KA) oil with 92% yield and 43% Faradaic efficiency (FE). At the anode, KA is electrooxidized into AA on CuCo2O4 catalysts, achieving a maximum of 85% yield and 84% FE. Experimental and theoretical studies reveal that the excellent catalytic activity can be ascribed to the enhanced absorption and activation capability of reactants on the bimetallic cooperative catalysts. A two-electrode flow electrolyzer for AA synthesis realizes a stable electrolysis at 2.5 A for over 200 h as well as 38.5% yield and 70.2% selectivity. This study offers a green and sustainable route for AA synthesis from lignin via paired electrolysis.
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Although laser technology brings convenience to production and daily life, it also causes high-energy damage. Therefore, there is an urgent need to develop optical limiting materials for laser protection. In this study, a novel nonlinear optical material, red/black phosphorus lateral heterojunction, is successfully prepared through solvothermal and ultrasonic treatment. Using the Z-scan method, the nonlinear optical properties of the red/black phosphorus heterojunction are determined at wavelengths of 532 and 1064 nm. These results indicate that the red/black phosphorus heterojunction exhibits reverse saturable absorption properties in 1.2.3-glycerol. Interestingly, the red/black phosphorus heterojunction shows an enhanced performance over red phosphorus by introducing the black phosphorus phase. Moreover, the red/black phosphorus heterojunction is doped into organically modified silicate gel glass with excellent broadband optical limiting performance. This study highlights the promising prospect of the red/black phosphorus heterojunction in the nonlinear optical and optical limiting fields.
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Polylactic acid (PLA) has attracted increasing interest as a sustainable plastic because it can be degraded into CO2 and H2O in nature. However, this process is sluggish, and even worse, it is a CO2-emitting and carbon resource waste process. Therefore, it is highly urgent to develop a novel strategy for recycling post-consumer PLA to achieve a circular plastic economy. Herein, we report a one-pot photoreforming route for the efficient and selective amination of PLA waste into value-added alanine using CoP/CdS catalysts under mild conditions. Results show the alanine production rate can reach up to 2.4â mmol gcat -1 h-1, with a high selectivity (>75 %) and excellent stability. Time-resolved transient absorption spectra (TAS) reveal that CoP can rapidly extract photogenerated electrons from CdS to accelerate proton reduction, favoring hole-dominated PLA oxidation to coproduce alanine. This study offers an appealing way for upcycling PLA waste and creates new opportunities for green synthesis of amino acids.
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Increasing atmospheric CO2 concentrations accompanied by intensifying drought markedly impact plant growth and physiology. This study aimed to explore the role of abscisic acid (ABA) in mediating the response of stomata to elevated CO2 (e[CO2]) and drought. Tomato plants with different endogenous ABA concentrations [Ailsa Craig (AC), the ABA-deficient mutant flacca, and ABA-overproducing transgenic tomato SP5] were grown in ambient (a[CO2], 400 µmol mol-1) and elevated (e[CO2],800 µmol mol-1) CO2 environments and subjected to progressive soil drying. Compared with a[CO2] plants, e[CO2] plants had significantly lower stomatal conductance in AC and SP5 but not in flacca. Under drought, e[CO2] plants had better water status and higher water use efficiency. e[CO2] promoted the accumulation of ABA in leaves of plants subjected to drought, which coincided with the up-regulation of ABA biosynthetic genes and down-regulation of ABA metabolic genes. Although the increase of ABA induced by drought in flacca was much less than in AC and SP5, flacca accumulated large amounts of ethylene, suggesting that in plants with ABA deficiency, ethylene might play a compensatory role in inducing stomatal closure during soil drying. Collectively, these findings improve our understanding of plant performance in a future drier and higher-CO2 environment.
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Abscisic acid (ABA) plays a vital role in the induction of low temperature tolerance in plants. To understand the molecular basis of this phenomenon, we performed a proteomic analysis on an ABA-deficit mutant barley (Az34) and its wild type (cv Steptoe) under control conditions (25/18 °C) and after exposure to 0 °C for 24 h. Most of the differentially abundant proteins were involved in the processes of photosynthesis and metabolisms of starch, sucrose, carbon, and glutathione. The chloroplasts in Az34 leaves were more severely damaged, and the decrease in Fv/Fm was larger in Az34 plants compared with WT under low temperature. Under low temperature, Az34 plants possessed significantly higher activities of ADP-glucose pyrophosphorylase, fructokinase, monodehydroascorbate reductase, and three invertases, but lower UDP-glucose pyrophosphorylase activity than WT. In addition, concentrations of proline and soluble protein were lower, while concentration of H2O2 was higher in Az34 plants compared to WT under low temperature. Collectively, the results indicated that ABA deficiency induced modifications in starch and sucrose biosynthesis and sucrolytic pathway and overaccumulation of reactive oxygen species were the main reason for depressed low temperature tolerance in barley, which provide novel insights to the response of barley to low temperature under future climate change.
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Ácido Abscísico , Hordeum , Ácido Abscísico/metabolismo , Hordeum/metabolismo , Proteómica , Peróxido de Hidrógeno/metabolismo , Fotosíntesis , Almidón/metabolismo , Sacarosa/metabolismo , Oxidación-Reducción , Homeostasis , Regulación de la Expresión Génica de las PlantasRESUMEN
Electro-reforming of Polyethylene-terephthalate-derived (PET-derived) ethylene glycol (EG) into fine chemicals and H2 is an ideal solution to address severe plastic pollution. Here, we report the electrooxidation of EG to glycolic acid (GA) with a high Faraday efficiency and selectivity (>85 %) even at an industry-level current density (600â mA cm-2 at 1.15â V vs. RHE) over a Pd-Ni(OH)2 catalyst. Notably, stable electrolysis over 200â h can be achieved, outperforming all available Pd-based catalysts. Combined experimental and theoretical results reveal that 1)â the OH* generation promoted by Ni(OH)2 plays a critical role in facilitating EG-to-GA oxidation and removing poisonous carbonyl species, thereby achieving high activity and stability; 2)â Pd with a downshifted d-band center and the oxophilic Ni can synergistically facilitate the rapid desorption and transfer of GA from the active Pd sites to the inactive Ni sites, avoiding over-oxidation and thus achieving high selectivity.
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Two wheat genotypes forming high and low biomass (HB and LB), exhibiting differential expression of an isoflavone reductase-like (IRL) gene, and resulting in contrasting grain yield under heat stress field conditions, were analyzed in detail for their responses under controlled heat and elevated CO2 conditions. Significant differences in IRL expression between the two lines were hypothesized to be the basis of their differential performance under the tested conditions and their stress tolerance potential. By a holistic approach integrating advanced cell physiological phenotyping of the antioxidative and phytohormone system in spikes and leaves with measurements of ecophysiological and agronomic traits, the genetic differences of the genotypes in IRL expression were assessed. In response to heat and elevated CO2, the two genotypes showed opposite regulation of IRL expression, which was associated with cytokinin concentration, total flavonoid contents, activity of superoxide dismutase, antioxidant capacity and photosynthetic rate in leaves and cytokinin concentration and ascorbate peroxidase activity in spikes. Our study showed that IRL expression is associated with wheat yield performance under heat stress at anthesis, mediated by diverse physiological mechanisms. Hence, based on our results, the IRL gene is a promising candidate for developing genetic markers for breeding heat-tolerant wheat.
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With increasing plastic production and consumption, large amounts of polystyrene nanoplastics are accumulated in soil due to improper disposal causing pollution and deleterious effects to environment. However, little information is available about how to alleviate the adverse impacts of nanoplastics on crops. In this study, the involvement of melatonin in modulating nanoplastic uptake, translocation, and toxicity in wheat plant was investigated. The results demonstrated that exogenous melatonin application reduced the nanoplastic uptake by roots and their translocation to shoots via regulating the expression of genes associated with aquaporin, including the upregulation of the TIP2-9, PIP2, PIP3, and PIP1.2 in leaves and TIP2-9, PIP1-5, PIP2, and PIP1.2 in roots. Melatonin activated the ROS scavenging system to maintain a better redox homeostasis and ameliorated the negative effects of nanoplastics on carbohydrate metabolism, hence ameliorated the plant growth and enhanced the tolerance to nanoplastics toxicity. This process was closely related to the exogenous melatonin application induced melatonin accumulation in leave. These results suggest that melatonin could alleviate the adverse effects of nanoplastics on wheat, and exogenous melatonin application might be used as a promising management strategy to sustain crop production in the nanoplastic-polluted soils.
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Melatonina , Triticum , Melatonina/farmacología , Microplásticos , Hojas de la Planta , PoliestirenosRESUMEN
Although domestic wastewater and its reclaimed water are alternative water resources in arid region, investigation of their negative effect must be done to prevent environmental pollution. In this paper, a short-term column experiment was conducted to simulate the infiltration process of wastewater in desert soil. Alfalfa was planted and irrigated with fresh water for control (CK), tertiary treated domestic wastewater (TTW), secondary treated domestic wastewater (STW) and raw domestic wastewater untreated (RW). The effect of wastewater application on desert soil, drainage and plant properties was evaluated. Experimental results demonstrated that the tested desert soil has no soil structure, organic matter, nor microbial community while possess high infiltration rate. The use of wastewater significantly improved plant growth, and the biomass of TTW, RW, STW were 5.5, 4.3, 2.9 times of CK. The infiltration rate of water in bare soil was high (high to low: TTW, CK, RW, STW), while plant growth reduced infiltration rate (ca. 40% with TTW and RW). Wastewater irrigation and plant growth decreased soil zeta potential, while increased formation of aggregates and bacterial abundance and diversity in soil. Top soil (0-30 cm) accumulation of nitrogen (N), phosphorus (P), organic matter and E. coli was evidenced and all could go down to deep soil and drainage with constant wastewater use. It was concluded that domestic wastewater had big potential in desert soil vegetation recovering and function restoration. Nevertheless, the N, salt, P and organic matter and E. coli in wastewater could give rise to desert soil and groundwater contamination if improper treatment was used.
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Riego Agrícola/métodos , Eliminación de Residuos Líquidos/métodos , Aguas Residuales/análisis , Bacterias , Escherichia coli , Agua Dulce , Microbiota , Plantas , Arena , Suelo/química , Microbiología del SueloRESUMEN
BACKGROUND: To improve our understanding about the physiological mechanism of grain yield reduction at anthesis, three spring wheat genotypes [L1 (advanced line), L2 (Vorobey) and L3 (Punjab-11)] having contrasting yield potential under drought in field were investigated under controlled greenhouse conditions, drought stress was imposed at anthesis stage by withholding irrigation until all plant available water was depleted, while well-watered control plants were kept at 95% pot water holding capacity. RESULTS: Compared to genotype L1 and L2, pronounced decrease in grain number (NGS), grain yield (GY) and harvest index (HI) were found in genotype L3, mainly due to its greater kernel abortion (KA) under drought. A significant positive correlation of leaf monodehydroascorbate reductase (MDHAR) with both NGS and HI was observed. In contrast, significant negative correlations of glutathione S-transferase (GST) and vacuolar invertase (vacInv) both within source and sink were found with NGS and HI. Likewise, a significant negative correlation of leaf abscisic acid (ABA) with NGS was noticed. Moreover, leaf aldolase and cell wall peroxidase (cwPOX) activities were significantly and positively associated with thousand kernel weight (TKW). CONCLUSION: Distinct physiological markers correlating with yield traits and higher activity of leaf aldolase and cwPOX may be chosen as predictive biomarkers for higher TKW. Also, higher activity of MDHAR within the leaf can be selected as a predictive biomarker for higher NGS in wheat under drought. Whereas, lower activity of vacInv and GST both within leaf and spike can be selected as biomarkers for higher NGS and HI. The results highlighted the role of antioxidant and carbohydrate-metabolic enzymes in the modulation of source-sink balance in wheat crops, which could be used as bio-signatures for breeding and selection of drought-resilient wheat genotypes for a future drier climate.
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Antioxidantes , Metabolismo de los Hidratos de Carbono , Sequías , Triticum/enzimología , Genotipo , Inflorescencia/enzimología , Inflorescencia/crecimiento & desarrollo , Hojas de la Planta/enzimología , Hojas de la Planta/crecimiento & desarrollo , Triticum/genética , Triticum/crecimiento & desarrolloRESUMEN
Solar-to-hydrogen (H2 ) conversion has been regarded as a sustainable and renewable technique to address aggravated environmental pollution and global energy crisis. The most critical aspect in this technology is to develop highly efficient and stable photocatalysts, especially metal-free photocatalysts. Recently, black phosphorus (BP), as a rising star 2D nanomaterial, has captured enormous attention in photocatalytic water splitting owing to its widespread optical absorption, adjustable direct band gap, and superior carrier migration characteristics. However, the rapid charge recombination of pristine BP has seriously limited its practical application as photocatalyst. The construction of BP-based semiconductor heterojunctions has been proven to be an effective strategy for enhancing the separation of photogenerated carriers. This Minireview attempts to summarize the recent progress in BP-based semiconductor heterojunctions for photocatalytic water splitting, including type-I and type-II heterojunctions, Z-Scheme systems, and multicomponent heterojunctions. Finally, a brief summary and perspective on the challenges and future directions in this field are also provided.
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Terminal drought stress poses a big challenge to sustain wheat grain production in rain-fed environments. This study aimed to utilize the genetically diverse pre-breeding lines for identification of genomic regions associated with agro-physiological traits at terminal stage drought stress in wheat. A total of 339 pre-breeding lines panel derived from three-way crosses of 'exotics × elite × elite' lines were evaluated in field conditions at Obregon, Mexico for two years under well irrigated as well as drought stress environments. Drought stress was imposed at flowering by skipping the irrigations at pre and post anthesis stage. Results revealed that drought significantly reduced grain yield (Y), spike length (SL), number of grains spikes-1 (NGS) and thousand kernel weight (TKW), while kernel abortion (KA) was increased. Population structure analysis in this panel uncovered three sub-populations. Genome wide linkage disequilibrium (LD) decay was observed at 2.5 centimorgan (cM). The haplotypes-based genome wide association study (GWAS) identified significant associations of Y, SL, and TKW on three chromosomes; 4A (HB10.7), 2D (HB6.10) and 3B (HB8.12), respectively. Likewise, associations on chromosomes 6B (HB17.1) and 3A (HB7.11) were found for NGS while on chromosome 3A (HB7.12) for KA. The genomic analysis information generated in the study can be efficiently utilized to improve Y and/or related parameters under terminal stage drought stress through marker-assisted breeding.
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Adaptación Biológica , Sequías , Carácter Cuantitativo Heredable , Estrés Fisiológico , Triticum/fisiología , Mapeo Cromosómico , Grano Comestible/fisiología , Marcadores Genéticos , Estudio de Asociación del Genoma Completo , Patrón de Herencia , Fitomejoramiento , Sitios de Carácter CuantitativoRESUMEN
The applications of ZnO nanoparticles in agriculture have largely contributed to crop growth regulation, quality enhancement, and induction of stress tolerance, while the underlying mechanisms remain elusive. Herein, the involvement of melatonin synthesis and metabolism in the process of nano-ZnO induced drought tolerance was investigated in maize. Drought stress resulted in the changes of subcellular ultrastructure, the accumulation of malondialdehyde and osmolytes in leaf. The nano-ZnO (100 mg L-1) application promoted the melatonin synthesis and activated the antioxidant enzyme system, which alleviated drought-induced damage to mitochondria and chloroplast. These changes were associated with upregulation of the relative transcript abundance of Fe/Mn SOD, Cu/Zn SOD, APX, CAT, TDC, SNAT, COMT, and ASMT induced by nano-ZnO application. It was suggested that modifications in endogenous melatonin synthesis were involved in the nano-ZnO induced drought tolerance in maize.
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Adaptación Biológica , Sequías , Melatonina/biosíntesis , Nanopartículas , Zea mays/efectos de los fármacos , Zea mays/fisiología , Óxido de Zinc/farmacología , Antioxidantes/metabolismo , Cloroplastos/metabolismo , Cloroplastos/ultraestructura , Metabolismo Energético , Regulación Enzimológica de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Mitocondrias/metabolismo , Mitocondrias/ultraestructura , Nanopartículas/química , Serotonina/biosíntesis , Óxido de Zinc/químicaRESUMEN
Many technologies have been developed to control agricultural non-point-source pollution (ANPSP). However, most reduce pollution from only a single source instead of considering an entire region with multiple pollution sources as a control unit. A pollutant reduction system for controlling ANPSP at a regional scale could be built by integrating technologies and the reuse of treated wastewater (TWR) and nutrients (NR) to protect the environment and achieve agricultural sustainability. The present study proposes four systematic schemes involving TWR for irrigation and NR in a region with three sources of ANPSP (crop farming, livestock and aquaculture). Subsequently, a multi-objective evaluation model is established based on the analytical hierarchy process (AHP) combined with grey relational analysis (GRA) to identify the optimal scheme considering six indices, namely, pollutant reductions (total nitrogen, TN; total phosphorous, TP; ammonium-nitrogen, NH4+-N; and chemical oxygen demand, COD) and costs (construction and operational costs). The Taihu Lake Basin suffers from some of the worst ANPSP in China, and a case study was conducted in a town with three ANPSP sources. Four systems were developed on the basis of suggested technologies and the scenarios of TWR and NR (Scenario I: no reuse, Scenario II: reuse of all livestock wastewater and manure, Scenario III: reuse of some aquaculture wastewater, and Scenario IV: reuse of all livestock wastewater and manure and some aquaculture wastewater). Pollutant reductions were calculated based on removal efficiency and pollutant loads, which were estimated from the local pollutant export coefficients and agricultural information (crop farming, livestock, and aquaculture). The costs were determined on the basis of the total pollutant reductions and unit cost. The results showed that the optimal system was the Scenario IV because it had the highest grey correlation degree among the four proposed systems. The optimal system met the irrigation water demand in Xinjian. In the optimal system, the removal efficiencies of the pollutants TN, TP, NH4+-N, and COD were 84.3%, 94.2%, 89.6% and 94.0%, respectively. In addition, the implementation of NR in the optimal system reduced the use of chemical fertilizers by nearly 81.7â¯kgâ¯N ha-1 and 39.9â¯kgâ¯P ha-1. The proposed methods provide a reference for the construction of a pollutant reduction system for controlling ANPSP in a multi-source region.
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Contaminantes Ambientales , Contaminantes Químicos del Agua , Agricultura , Animales , China , Monitoreo del Ambiente , Nitrógeno , FósforoRESUMEN
Black phosphorus (BP) has recently drawn attention in photocatalysis for its optical properties. However, limited by the rapid recombination of photogenerated carriers, the use of BP for photocatalytic water splitting still remains a huge challenge. Herein, we prepare a black/red phosphorus (BP/RP) hetero-phase junction photocatalyst by a wet-chemistry method to promote the interfacial charge separation and thus achieve Z-scheme photocatalytic water splitting without using sacrificial agents. The Z-scheme mechanism was confirmed by time-resolved transient absorption spectroscopy. This work provides a novel insight into the interface design of hetero-phase junction with atomic precision.
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Melatonin is involved in the regulation of carbohydrate metabolism and induction of cold tolerance in plants. The objective of this study was to investigate the roles of melatonin in modulation of carbon assimilation of wild-type wheat and the Chl b-deficient mutant ANK32B in response to elevated CO2 concentration ([CO2 ]) and the transgenerational effects of application of exogenous melatonin (hereafter identified as melatonin priming) on the cold tolerance in offspring. The results showed that the melatonin priming enhanced the carbon assimilation in ANK32B under elevated [CO2 ], via boosting the activities of ATPase and sucrose synthesis and maintaining a relatively higher level of total chlorophyll concentration in leaves. In addition, melatonin priming in maternal plants at grain filling promoted the seed germination in offspring by accelerating the starch degradation and improved the cold tolerance of seedlings through activating the antioxidant enzymes and enhancing the photosynthetic electron transport efficiency. These findings suggest the important roles of melatonin in plant response to future climate change, indicating that the melatonin priming at grain filling in maternal plants could be an effective approach to improve cold tolerance of wheat offspring at seedling stage.
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Clorofila/síntesis química , Clorofila/deficiencia , Melatonina/farmacología , Triticum/metabolismo , Carbono/metabolismo , Dióxido de Carbono/metabolismo , Clorofila/metabolismo , Triticum/efectos de los fármacosRESUMEN
Cold priming can alleviate the effects of subsequent cold stress on wheat plant growth. Melatonin plays a key role in cold stress response in plants. In this study, the effects of foliar melatonin application during recovery on the cold tolerance of cold primed wheat plants were investigated. It was found that both melatonin and cold priming increased the photosynthetic rate and stomatal conductance, enhanced the activities of antioxidant enzymes, and altered the related gene expressions in wheat under cold stress. Melatonin application is helpful for the photosynthetic carbon assimilation and membrane stability of the cold primed plants under cold stress. These results suggested that foliar melatonin application during recovery enhanced the cold priming induced tolerance to subsequent low temperature stress in wheat.
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Melatonina/farmacología , Termotolerancia/efectos de los fármacos , Triticum/efectos de los fármacos , Frío/efectos adversos , Respuesta al Choque por Frío/efectos de los fármacos , Triticum/crecimiento & desarrollo , Triticum/fisiologíaRESUMEN
Melatonin is involved in multiple plant developmental processes and various stress responses. To explore the roles of melatonin played as well as its association with abscisic acid (ABA) in a process of drought priming-induced cold tolerance (DPICT), a wild-type barley and its ABA-deficient mutant Az34 counterpart were selected for comparison, in which the effects of melatonin application (either foliarly or rhizospherically) and/or drought priming on the cold tolerance of both types of barleys were systematically investigated. It was demonstrated that the early drought priming induced an increase of endogenous melatonin production, which is not ABA dependent. In addition, exogenously applied melatonin resulted in higher ABA concentration in the drought-primed plants than in the nonprimed plants when exposed to cold stress, indicating that ABA responded in a drought-dependent manner. The interplay of melatonin and ABA leads to plants maintaining better water status. Drought priming-induced melatonin accumulation enhanced the antioxidant capacity in both chloroplasts and mitochondria, which sustained the photosynthetic electron transport in photosynthetic apparatus of the plants under cold stress. These results suggest that the exogenous melatonin application enhances the DPICT by modulating subcellular antioxidant systems and ABA levels in barley.
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Ácido Abscísico/genética , Frío , Respuesta al Choque por Frío/efectos de los fármacos , Hordeum/metabolismo , Melatonina/farmacología , Mutación , Ácido Abscísico/metabolismo , Respuesta al Choque por Frío/genética , Hordeum/genéticaRESUMEN
Effects of the arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis on plant growth, carbon (C) and nitrogen (N) accumulation, and partitioning was investigated in Triticum aestivum L. plants grown under elevated CO2 in a pot experiment. Wheat plants inoculated or not inoculated with the AM fungus were grown in two glasshouse cells with different CO2 concentrations (400 and 700 ppm) for 10 weeks. A (15)N isotope labeling technique was used to trace plant N uptake. Results showed that elevated CO2 increased AM fungal colonization. Under CO2 elevation, AM plants had higher C concentration and higher plant biomass than the non-AM plants. CO2 elevation did not affect C and N partitioning in plant organs, while AM symbiosis increased C and N allocation into the roots. In addition, plant C and N accumulation, (15)N recovery rate, and N use efficiency (NUE) were significantly higher in AM plants than in non-AM controls under CO2 enrichment. It is concluded that AM symbiosis favors C and N partitioning in roots, increases C accumulation and N uptake, and leads to greater NUE in wheat plants grown at elevated CO2.
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Dióxido de Carbono/metabolismo , Glomeromycota/fisiología , Micorrizas/fisiología , Nitrógeno/metabolismo , Simbiosis/efectos de los fármacos , Triticum/crecimiento & desarrollo , Triticum/metabolismo , Biomasa , Carbono/análisis , Glomeromycota/efectos de los fármacos , Marcaje Isotópico , Micorrizas/efectos de los fármacos , Triticum/microbiologíaRESUMEN
BACKGROUND: Mechanical wounding can cause morphological and developmental changes in plants, which may affect the responses to abiotic stresses. However, the mechano-stimulation triggered regulation network remains elusive. Here, the mechano-stimulation was applied at two different times during the growth period of wheat before exposing the plants to cold stress (5.6 °C lower temperature than the ambient temperature, viz., 5.0 °C) at the jointing stage. RESULTS: Results showed that mechano-stimulation at the Zadoks growth stage 26 activated the antioxidant system, and substantially, maintained the homeostasis of reactive oxygen species. In turn, the stimulation improved the electron transport and photosynthetic rate of wheat plants exposed to cold stress at the jointing stage. Proteomic and transcriptional analyses revealed that the oxidative stress defense, ATP synthesis, and photosynthesis-related proteins and genes were similarly modulated by mechano-stimulation and the cold stress. CONCLUSIONS: It was concluded that mechano-stimulated modifications of the chloroplast antioxidant system and proteome changes are related to cold tolerance in wheat. The findings might provide deeper insights into roles of reactive oxygen species in mechano-stimulated cold tolerance of photosynthetic apparatus, and be helpful to explore novel approaches to mitigate the impacts of low temperature occurring at critical developmental stages.