Isolation and genome-wide analysis of the novel Acinetobacter baumannii bacteriophage vB_AbaM_AB3P2.

A lytic Acinetobacter baumannii phage, isolate vB_AbaM_AB3P2, was isolated from a sewage treatment plant in China. A. baumannii phage vB_AbaM_AB3P2 has a dsDNA genome that is 44,824 bp in length with a G + C content of 37.75%. Ninety-six open reading frames were identified, and no genes for antibiotic resistance or virulence factors were found. Genomic and phylogenetic analysis of this phage revealed that it represents a new species in the genus Obolenskvirus. Phage vB_AbaM_AB3P2 has a short latent period (10 min) and high stability at 30-70°C and pH 2-10 and is potentially useful for controlling multi-drug-resistant A. baumannii.

How does temperature play a role in the storage of extracellular vesicles?

Extracellular vesicles (EVs) contain specific proteins, lipids, and nucleic acids that can be passed to other cells as signal molecules to alter their function. However, there are many problems and challenges in the conversion and clinical application of EVs. Storage and protection of EVs is one of the issues that need further research. To adapt to potential clinical applications, this type of problem must be solved. This review summarizes the storage practices of EVs in recent years, and explains the impact of temperature on the quality and stability of EVs during storage based on current research, and explains the potential mechanisms involved in this effect as much as possible.

How does social integration work when older migrants obtain health services from community? Evidence from national database in China.

Background: The roles of community are often overlooked when studying the older migrants' health issues, and more importantly, the mediating effect of social integration on the health of older migrants were rarely investigated empirically. Methods: This study developed comprehensive index to explore this relationship. With data from the 2017 China Migrants Dynamic Survey, the study first examined the potential linkage between community-based health services and the health of older migrants. Ordered logit regressions was carried to investigate whether the self-rated health of older migrants is related to health education and health records provided by community, then the Causal Stepwise Regression and bootstrap method was used to looked into the potential mediation effect. Results: The findings showed that older migrants with more community-based health education had higher self-rated health (ß = 0.038, SE = 0.009, p < 0.001). However, the community-based health records were not associated with older migrants' health. Moreover, higher levels of social integration were associated with community health education (ß = 0.142, SE = 0.014, p < 0.001), and social integration was positively associated with older migrants' health (ß = 0.039, SE = 0.002, p = 0.024), indicating the mediation role of social integration. Conclusion: The vital role of community-based health education in improving the health of older migrants was found, and social integration plays a mediating role.

Abnormal expression of key genes and proteins in the canonical Wnt/ß-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis.

To investigate the molecular pathogenesis of the canonical Wnt/ß-catenin pathway in exercise-induced osteoarthritis (OA), 30 male healthy Sprague Dawley rats were divided into three groups (control, normal exercise­induced OA and injured exercise­induced OA groups) in order to establish the exercise­induced OA rat model. The mRNA and protein expression levels of Runx­2, BMP­2, Ctnnb1, Sox­9, collagen Ⅱ, Mmp­13, Wnt­3a and ß­catenin in chondrocytes were detected by reverse transcription­quantitative polymerase chain reaction, western blotting and immunohistochemical staining. The mRNA levels of Runx­2, BMP­2 and Ctnnb1 were upregulated in the normal exercise­induced OA and injured exercise­induced OA groups; while Runx­2 and BMP­2 were upregulated in the injured exercise­induced OA group when compared with the normal exercise­induced OA group. The protein levels of Mmp­13, Wnt­3a and ß­catenin were increased and collagen Ⅱ was reduced in the normal exercise­induced OA and injured exercise­induced OA groups. Ctnnb1, Wnt­3a and ß­catenin, which are key genes and proteins in the canonical Wnt/ß­catenin pathway, were abnormally expressed in chondrocytes of the exercise­induced OA rat model. Ctnnb1, ß­catenin and Wnt­3a were suggested to participate in the pathogenesis of exercise­induced OA by abnormally activating the Wnt/ß­catenin pathway during physical exercise due to excessive pressure. The results of the present study may provide an improved understanding of the pathogenesis of exercise-induced OA.