Preparation of Zinc Oxide with Core-Shell Structure and Its Application in Rubber Products.

Zinc oxide is a crucial component in rubber products, but its excessive usage can lead to environmental damage. As a result, reducing the amount of zinc oxide in products has become a critical issue that many researchers aim to address. This study employs a wet precipitation method to prepare ZnO particles with different nucleoplasmic materials, resulting in ZnO with a core-shell structure. The prepared ZnO underwent XRD, SEM, and TEM analysis, indicating that some of the ZnO particles were loaded onto the nucleosomal materials. Specifically, ZnO with a silica core-shell structure demonstrated 11.9% higher tensile strength, 17.2% higher elongation at break, and 6.9% higher tear strength compared to the indirect method of ZnO preparation. The core-shell structure of ZnO also helps reduce its application in rubber products, thereby achieving the dual objective of protecting the environment and improving the economic efficiency of rubber products.

TiO2-modified MoS2 monolayer films enable sensitive NH3 sensing at room temperature.

The research and development of high-performance NH3 sensors are of great significance for environment monitoring and disease diagnosis applications. Two-dimensional (2D) MoS2 nanomaterials have exhibited great potential for building room-temperature (RT) NH3 sensors but still suffer from relatively low sensitivity. Herein, the TiO2-modified monolayer MoS2 films with controllable TiO2 loading contents are fabricated by a facile approach. A remarkable enhancement in the RT NH3 sensing performance is achieved after the n-n hetero-compositing of the TiO2/MoS2 system. The device with 95% surface coverage of TiO2 shows enhanced sensor response, low detection limit (0.5 ppm), wide detection range (0.5-1000 ppm), good repeatability, and superior selectivity against other gases. In situ Kelvin potential force microscopy results revealed that the TiO2 modification not only improved the surface reactivity of the sensing layers but also contributed to the NH3 sensing performance by serving as the "gas-gating" layers that modulated the electron depletion layer and the conductivity of the MoS2 films. Such an n-n hetero-compositing strategy can provide a simple and cost-effective approach for developing high-performance NH3 sensors based on 2D semiconductors.

The impact of criminal law regulation-based business environment optimization on entrepreneurial spirit and enterprise development.

The purpose is to explore the impact of the business environment optimization by Criminal Law Regulation (CLR) on Entrepreneurial Spirit (ES) and Enterprise Development (ED) and to provide a reference for subsequent related research. Based on this, this work first makes a detailed analysis of the business environment and CLR. Second, the research hypotheses are put forward, and the conceptual model is proposed. At the same time, a Questionnaire Survey (QS) is designed to analyze the business environment, ES, and ED, and their relationships. Finally, a Structural Equation Model (SEM) is constructed and the CLR-optimized business environment is used as the intermediary variable to explore the impact of the business environment on ES and ED. Then, 200 QSs (recovering 192 valid ones) are distributed to investigate entrepreneurs' attitudes toward ES and ED in different regions. Statistical analysis and independent t-tests are performed on the survey results to judge the relationship between variables. The results of empirical analysis show that (1) The significance coefficient P of ES and ED is 0.005 < 0.01, and the scores of ES and ED of large enterprises are 132.7864 and 142.3243, respectively, which are the highest. Therefore, CLR-optimized business regulation has a significant positive impact on the ED. (2) The influence coefficient of CLR-optimized business regulation and ES is 0.60, and the influence coefficient of CLR-optimized business regulation and ED is 0.75. Therefore, CLR-optimized business regulation plays a positive role in the development of ES. CLR-optimized business regulation plays a regulating role between ES and ED. (3) CLR-optimized business regulation has a significant positive impact on the formation of ES. The policy enlightenment of this work mainly has three points. First, optimizing the business environment can stimulate and protect ES, thus improving the quality of economic growth. All regions should promote "mass entrepreneurship and innovation" and high-quality economic development by improving the convergence of economic policies and building a legal and market-oriented business environment. Second, all regions should implement dynamic and differentiated policies to optimize the business environment's spatial pattern in Chinese cities. Third, there is a need to further strengthen the construction of new infrastructure through cutting-edge information technologies, such as Fifth Generation (5G) mobile communication, Big Data, and Artificial Intelligence (AI).

Experiment and observation on invasion of brain glioma in vivo.

Research on invasion and metastasis of glioma in vivo was performed by implanting C6 glioma cells transfected with enhanced green fluorescent protein (EGFP) gene into the brain of SD rats. Firstly, C6 glioma cells were transfected with a plasmid vector (pEGFP-N3) containing the EGFP gene. Stable EGFP-expressing clones were isolated and examination for these cells by flow cytometry and electron microscope was done. Secondly, EGFP-expressing cells were stereotactically injected into the brain parenchyma of SD rats to establish xenotransplanted tumor. Four weeks later rats were killed and continuous brain sections were examined using fluorescence microscopy after adjacent sections were examined by immunohistochemistry or routine hematoxylin and eosin staining for the visualization and detection of tumor cell invasion. Xenotransplanted tumor was primarily cultured to determine the storage of EGFP gene in vivo. The results showed that EGFP-transfected C6 glioma cells maintained stable high-level EGFP expression in the central nervous system during their growth in vivo. EGFP fluorescence clearly demarcated the primary tumor margin and readily allowed for the visualization of distant micrometastasis and invasion on the single-cell level. Small locally invasive foci, including those immediately adjacent to the leading invasive edge of the tumor, were virtually undetectable by routine hematoxylin and eosin staining and immunohistochemistry. These results suggested that EGFP-transfected C6 cells can be visualized by fluorescence microscopy after intracranial implantation. This model is an excellent experimental animal model in research on invasion and metastasis of brain glioma in vivo.

[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo].

OBJECTIVE: To investigate the effects of recombinant human bone morphogenic protein-2 (rhBMP-2) and hyaluronic acid (HA) on the invasion and metastasis of rat brain glioma in vivo. METHODS: C6 rat glioma cells were transfected with the plasmid vector named pEGFP-N3 which contained an enhanced green fluorescent protein (EGFP) gene. The stable cell clones which expressed EGFP protein were stereotactically injected into the brain parenchyma of SD rats in order to establish a xenotransplanted tumor model. After rhBMP-2 and HA were administered in the xenotransplanted tumor model at different dosages, their effects on glioma invasion and metastasis were observed by pathology, flow cytometry fluoresclnec, and electronmicvoscopy. RESULTS: EGFP-transfected C6 glioma cells gave off green fluorescence in vivo and in vitro. Area with tumor and area without tumor could be easily distinguished by fluorescence microscopy. Even distant micrometastasis and invasion at single-cell level could be detected. 10 microliter rhBMP-2 of the concentration of 5 microgram/ml inhibited C6 tumor invasion, while 10 microliter hyaluronic acid of the concentration of 100 microgram/ml evidently promoted the tumor invasion. CONCLUSION: EGFP-transfected C6 cells can be transplanted into rats' brain so as to establish an excellent experimental animal model in the research on invasion and metastasis of brain glioma in vivo. RhBMP-2 inhibits tumor invasion, while HA evidently promotes its invasion.

[Effects of hypertonic saline on erythrocyte adherence function and bacterial infection of hemorrhagic shock rabbits].

AIM: To explore the effect of hypertonic saline on the erythrocyte adherence function and bacterial infection of hemorrhagic shock rabbits. METHODS: 60 Japanese rabbits were randomly divided into 6 groups, 10 for each group. Artery catheterization and heparin were given to the rabbits in group 1 (sham shock group). Hemorrhagic shock model was set up by bleeding resulting from carotid artery catheter in group 2 (normal saline group )and group 3 (hypertonic saline group). 30 minutes after shock, the rabbits in group 1 and group 2 were treated with normal saline and balanced salt solution containing 1 x 10(9)/kg E.coli, respectively. And the rabbits in group 3 were treated with 75 g/L NaCl solution and balanced salt solution containing 1 x 10(9)/kg E.coli. Then the survival rates of the rabbits in group 1-3 were observed. Rabbits in group 4-6 were same treatment as received, group 1-3, respectively, except that there was no E.coli in balanced salt solution. The erythrocyte immune adherence function of rabbits in group 4-6 were detected 5 hours after shock by RBC-C3bR and RBC-IC rosette forming assays. RESULTS: The survival rate of rabbits in hypertonic saline group was significantly higher than that in normal saline group. The RBC-C3bR rosette forming rate of the normal saline treated rabbits were pronouncedly decreased, while RBC-IC rosette forming rate was notably elevated, as compared with those of either sham shock group or hypertonic saline group(P<0.01). Hypertonic saline markedly increased RBC C3bR rosette forming rate. CONCLUSION: The above findings suggest that hypertonic salt solution can remarkably improve the depressed erythrocyte immune adherence function and enhance the rabbit's resistance to E.coli challenge after hemorrhagic shock.