The Arsenite Oxidation Potential of Native Microbial Communities from Arsenic-Rich Freshwaters.

Microorganisms play an important role in speciation and mobility of arsenic in the environment, by mediating redox transformations of both inorganic and organic species. Since arsenite [As(III)] is more toxic than arsenate [As(V)] to the biota, the microbial driven processes of As(V) reduction and As(III) oxidation may play a prominent role in mediating the environmental impact of arsenic contamination. However, little is known about the ecology and dynamics of As(III)-oxidizing populations within native microbial communities exposed to natural high levels of As. In this study, two techniques for single cell quantification (i.e., flow cytometry, CARD-FISH) were used to analyze the structure of aquatic microbial communities across a gradient of arsenic (As) contamination in different freshwater environments (i.e., groundwaters, surface and thermal waters). Moreover, we followed the structural evolution of these communities and their capacity to oxidize arsenite, when experimentally exposed to high As(III) concentrations in experimental microcosms. Betaproteobacteria and Deltaproteobacteria were the main groups retrieved in groundwaters and surface waters, while Beta and Gammaproteobacteria dominated the bacteria community in thermal waters. At the end of microcosm incubations, the communities were able to oxidize up to 95 % of arsenite, with an increase of Alphaproteobacteria in most of the experimental conditions. Finally, heterotrophic As(III)-oxidizing strains (one Alphaproteobacteria and two Gammaproteobacteria) were isolated from As rich waters. Our findings underlined that native microbial communities from different arsenic-contaminated freshwaters can efficiently perform arsenite oxidation, thus contributing to reduce the overall As toxicity to the aquatic biota.

Probabilistic Contaminant Source Assessment-Getting the Most Out of Field Measurements.

This paper describes a methodology for undertaking probabilistic investigations into the locations at which contaminants have leaked into a groundwater system. The methodology is built with highly parameterized, stochastic history-matching in mind. It is able to reduce uncertainties associated with estimates of subsurface hydraulic properties at the same time as it reduces uncertainties associated with inferences of contaminant sources. Particles are used to simulate contaminant movement. This reduces simulator execution time while increasing simulator stability. Borehole measurements of groundwater chemistry are endowed with a binary classification that indicates the presence, or otherwise, of a contaminant plume. This classification is transferred to passing particles as a detect or nondetect status awarded to their trajectories. Because this status is continuous with respect to model parameters, the latter can be adjusted in order to ensure that the same trajectory cannot possess both a detect status and a nondetect status. Particle trajectory statuses can be assigned to model cells from which they are released. By calculating cell statistics using a large number of history-match-constrained, stochastic parameter fields, probability maps can be drawn. We illustrate two of these. The first maps the probability that a contaminant sourced at a particular location will go undetected by the current observation network. The second maps the probability that a contaminant source cannot exist at a particular location. The method is extended to examine the worth of supplementing an existing observation network with new wells.

Sustainable Yield of a Hydrothermal Area: From Theoretical Concepts to the Practical Approach.

Sustainable use of groundwater in the hydrothermal area of Viterbo (Central Italy) was analyzed. In this area, multipurposes utilization of groundwater coexists: several thermal springs and wells supply spas and public pools, cold and fresh water is used for irrigation and drinking-water. Starting from theoretical concepts, a management plan has been developed to ensure groundwater sustainability in response to the increased demand of withdrawal from thermal wells, by integrating previous hydrogeological studies, new investigations and a new finite-difference model. The most stringent constraints considered are: to maintain the quality of thermal and fresh waters, to limit the effects on the hydraulic equilibrium existing between overlapping aquifers, to ensure a significant flow to the natural thermal springs and the quality and flow rate of the spring used for drinking purposes. The practical approach included identification of the maximum pumping rate from the wells of the spas, analysis of the response time of the system under development and drafting of a safeguard and monitoring plan. The case examined takes into account the complexity of the task in defining practical measures for groundwater management on the basis of theoretical concepts of its sustainable use. A participative approach among the different water decision-makers and adaptive management in the use of groundwater resources with different quality represent the key points to overcome conflicts between different users, with the awareness of the ineludible uncertainties of the hydrogeological model.