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Eur Rev Med Pharmacol Sci ; 19(19): 3593-604, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26502849

RESUMEN

OBJECTIVE: MicroRNAs (miRNAs) function as negative regulators for the expression of genes involved in cancer metastasis. The aim of this study was to investigate the potential role of miR-98 in gliomas and validate its regulatory mechanism. PATIENTS AND METHODS: Cell viability assays are used to measure proliferation of cell. mRNA expression is measured by qRT-PCR. Western blot analysis is used to measure protein expression. RESULTS: Functional studies showed that miR-98 overexpression inhibited glioma migration and invasion, but had no effect on the cell viability. An enhanced green fluorescent protein reporter assay, quantitative RT-PCR, and a western blot analysis confirmed that miR-98 suppressed the expression of IκB kinase (IKKε) by directly targeting its 3'-untranslated region, also, the NF-κB p65 nuclear translocation and matrix metalloproteinase (MMP)-9 expression were significantly arrested in glioma cells treated with miR-98 mimics. Accordingly, the overexpression of IKKε or NF-κB p65 can restore cell migration and invasion after being inhibited by miR-98, and can restore NF-κB p65 nuclear translocation as well as increase MMP-9 expression. CONCLUSIONS: These findings demonstrated that miR-98 functions as a tumor suppressor in gliomas. Furthermore, miR-98 may act as a potential therapeutic biomarker for glioma patients.


Asunto(s)
Neoplasias Encefálicas/metabolismo , Regulación hacia Abajo/fisiología , Regulación Neoplásica de la Expresión Génica , Glioma/metabolismo , Quinasa I-kappa B/metabolismo , MicroARNs/biosíntesis , Adulto , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/patología , Línea Celular Tumoral , Movimiento Celular/genética , Supervivencia Celular/genética , Femenino , Glioma/genética , Glioma/patología , Humanos , Quinasa I-kappa B/genética , Masculino , MicroARNs/genética , Persona de Mediana Edad , Invasividad Neoplásica/genética
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